Restriction map for
pFN21AB8703
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MseI MslI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 398 TCCGGA T^CCGGA
AcsI 967 GAATTC [GA]^AATT[CT]
AflIII 1223 ACATGT A^C[GA][CT]GT
ApaI 652 GGGCCC GGGCC^C
ApoI 967 GAATTC [GA]^AATT[CT]
Asp700I 967 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 602 GTGCTC G[AT]GC[AT]^C
BalI 1568 TGGCCA TGG^CCA
BbeI 455 GGCGCC GGCGC^C
BbrPI 1156 CACGTG CAC^GTG
BbsI 92 GTCTTC GAAGAC(2/6)
BcgI 355 CGAAAAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 598 CTAG C^TAG
BglII 1685 AGATCT A^GATCT
BpuAI 92 GTCTTC GAAGAC(2/6)
BsaAI 1156 CACGTG [CT]AC^GT[GA]
BsaWI 398 TCCGGA [AT]^CCGG[AT]
BseAI 398 TCCGGA T^CCGGA
BsgI 819 GTGCAG GTGCAG(16/14)
BsiHKAI 602 GTGCTC G[AT]GC[AT]^C
BspEI 398 TCCGGA T^CCGGA
BspLU11I 1223 ACATGT A^CATGT
DrdI 684 GACTCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 967 GAATTC G^AATTC
HindIII 445 AAGCTT A^AGCTT
KasI 455 GGCGCC G^GCGCC
MaeI 598 CTAG C^TAG
MluNI 1568 TGGCCA TGG^CCA
MroI 398 TCCGGA T^CCGGA
MscI 1568 TGGCCA TGG^CCA
NarI 455 GGCGCC GG^CGCC
PflMI 148 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1156 CACGTG CAC^GTG
PmlI 1156 CACGTG CAC^GTG
PshAI 122 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1613 AGTACT AGT^ACT
SfcI 1495 CTACAG C^T[GA][CT]AG
SfoI 455 GGCGCC GGC^GCC
SmaI 566 CCCGGG CCC^GGG
StuI 892 AGGCCT AGG^CCT
TaqI 354 TCGA T^CGA
Tsp509I 968 AATT ^AATT
TthHB8I 354 TCGA T^CGA
Van91I 148 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 566 CCCGGG C^CCGGG
XmaI 566 CCCGGG C^CCGGG
XmnI 967 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 151 GATCC GGATC(4/5)
1089 GATCC
AvaI 566 CCCGGG C^[CT]CG[GA]G
711 CTCGGG
BanII 652 GGGCCC G[GA]GC[CT]^C
858 GGGCTC
BglI 458 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
645 GCCCTCTGGGC
BpmI 1027 CTGGAG CTGGAG(16/14)
1384 CTGGAG
BsaI 1232 GAGACC GGTCTC(1/5)
632 GGTCTC
BsoBI 566 CCCGGG C^[CT]CG[GA]G
711 CTCGGG
BspMI 361 ACCTGC ACCTGC(4/8)
943 ACCTGC
BsrDI 812 GCAATG GCAATG(2/0)
1115 GCAATG
BsrFI 467 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
BstAPI 812 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1659 GCAGGACATGC
BstXI 1334 CCAAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCATCCACCTGG
BstYI 150 AGATCC [GA]^GATC[CT]
1685 AGATCT
Cfr10I 467 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
EaeI 909 CGGCCA [CT]^GGCC[GA]
1568 TGGCCA
EcoNI 154 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
924 CCTGCGAGAGG
HaeII 371 AGCGCC [GA]GCGC^[CT]
455 GGCGCC
MflI 150 AGATCC [GA]^GATC[CT]
1685 AGATCT
NaeI 467 GCCGGC GCC^GGC
907 GCCGGC
NgoMIV 467 GCCGGC G^CCGGC
907 GCCGGC
PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT]
1180 GGGACCT
PvuII 310 CAGCTG CAG^CTG
1084 CAGCTG
SexAI 1211 ACCAGGT A^CC[AT]GGT
1235 ACCTGGT
SmlI 72 CTCAAG C^T[CT][GA]AG
124 CTCAAG
XcmI 1064 CCAGGAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1166 CCATGGATGACTTGG
XhoII 150 AGATCC [GA]^GATC[CT]
1685 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspI AvaII BanI BbvCI BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
Tth111I