(Circular) (Six-base) MAPSORT of: pFN21AB8703.seq Check: 7072 from: 1 to: 6427 pFN21AB8703 6427 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4490 5874 6142 1632 Size: 2858 1384 268 1917 Fragments arranged by size: 2858 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 3228 5326 6303 1203 Size: 2025 2098 977 1327 Fragments arranged by size: 2098 2025 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2541 1184 Size: 1357 5070 AlwNI CAG_nnn'CTG Cuts at: 2159 3260 3597 4170 5742 2159 Size: 1101 337 573 1572 2844 Fragments arranged by size: 2844 1572 1101 573 337 ApaI G_GGCC'C Cuts at: 2661 2661 Size: 6427 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 2972 3736 3962 4114 6223 1493 Size: 1479 764 226 152 2109 1697 Fragments arranged by size: 2109 1697 1479 764 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2571 2716 3752 1870 Size: 73 628 145 1036 4545 Fragments arranged by size: 4545 1036 628 145 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 2539 2788 3748 5203 Size: 325 217 627 673 79 249 960 1455 Cuts at: 5203 618 Size: 1842 Fragments arranged by size: 1842 1455 960 673 627 325 249 217 79 BanII G_rGCy'C Cuts at: 1391 2661 2867 3746 1391 Size: 1270 206 879 4072 Fragments arranged by size: 4072 1270 879 206 BbeI G_GCGC'C Cuts at: 2464 2464 Size: 6427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2090 928 Size: 971 191 5265 Fragments arranged by size: 5265 971 191 BbvCI CC'TCA_GC Cuts at: 2221 2619 3005 2221 Size: 398 386 5643 Fragments arranged by size: 5643 398 386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2349 2383 4618 4652 1903 Size: 34 412 34 2235 34 3678 Fragments arranged by size: 3678 2235 412 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2469 2656 5038 Size: 107 122 71 631 605 797 187 2382 Cuts at: 5038 136 Size: 1525 Fragments arranged by size: 2382 1525 797 631 605 187 122 107 71 BglII A'GATC_T Cuts at: 3690 6422 3690 Size: 2732 3695 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2652 2661 4488 5644 1790 Size: 862 9 1827 1156 2573 Fragments arranged by size: 2573 1827 1156 862 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4158 5116 442 Size: 416 53 83 399 24 2741 958 1753 Fragments arranged by size: 2741 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2655 2655 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3053 3410 5087 1239 Size: 1814 357 1677 2579 Fragments arranged by size: 2579 1814 1677 357 Bpu10I CC'TnA_GC Cuts at: 2043 2221 2619 3005 3480 2043 Size: 178 398 386 475 4990 Fragments arranged by size: 4990 475 398 386 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2062 2114 4552 5417 5715 2062 Size: 52 2438 865 298 2774 Fragments arranged by size: 2774 2438 865 298 52 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2643 3231 5090 882 Size: 617 1144 588 1859 2219 Fragments arranged by size: 2219 1859 1144 617 588 BsaAI yAC'GTr Cuts at: 493 1204 3163 4218 493 Size: 711 1959 1055 2702 Fragments arranged by size: 2702 1959 1055 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2188 2461 2531 4234 Size: 53 83 186 936 655 273 70 1703 Cuts at: 4234 4616 5251 275 Size: 382 635 1451 Fragments arranged by size: 1703 1451 936 655 635 382 273 186 83 70 53 BsaWI w'CCGG_w Cuts at: 2403 4860 5258 5532 5679 6090 2403 Size: 2457 398 274 147 411 2740 Fragments arranged by size: 2740 2457 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3228 3258 1178 Size: 30 165 30 1825 30 4347 Fragments arranged by size: 4347 1825 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2261 2264 2267 2270 2288 2691 2951 Size: 1063 12 3 3 3 18 403 260 Cuts at: 2951 1186 Size: 4662 Fragments arranged by size: 4662 1063 403 260 18 12 3 3 3 BseYI C'CCAG_C Cuts at: 2068 2317 2328 2514 2593 3660 5630 6126 2068 Size: 249 11 186 79 1067 1970 496 2369 Fragments arranged by size: 2369 1970 1067 496 249 186 79 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2845 1163 Size: 573 1109 4745 Fragments arranged by size: 4745 1109 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2611 4488 4573 5644 2611 Size: 1877 85 1071 3394 Fragments arranged by size: 3394 1877 1071 85 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2611 2652 2661 2867 3746 4488 4573 Size: 399 821 41 9 206 879 742 85 Cuts at: 4573 5644 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 879 821 742 399 206 85 41 9 BspEI T'CCGG_A Cuts at: 2403 6090 2403 Size: 3687 2740 BspHI T'CATG_A Cuts at: 4316 6046 4316 Size: 1730 4697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2375 2957 3782 844 Size: 1531 582 825 3489 Fragments arranged by size: 3489 1531 825 582 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2824 3127 4922 5096 65 Size: 1183 1576 303 1795 174 1396 Fragments arranged by size: 1795 1576 1396 1183 303 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2472 2912 5071 5258 1700 Size: 110 662 440 2159 187 2869 Fragments arranged by size: 2869 2159 662 440 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2823 3670 794 Size: 2029 847 3551 Fragments arranged by size: 3551 2029 847 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6427 BstXI CCAn_nnnn'nTGG Cuts at: 3346 3388 3346 Size: 42 6385 BstYI r'GATC_y Cuts at: 1972 2155 3690 3757 4124 4509 4526 5978 6064 Size: 183 1535 67 367 385 17 1452 86 Cuts at: 6064 6076 6422 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1535 1452 385 367 346 183 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2664 3126 3171 513 Size: 552 1599 462 45 3769 Fragments arranged by size: 3769 1599 552 462 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 3709 3943 4737 4757 3709 Size: 234 794 20 5379 Fragments arranged by size: 5379 794 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2119 2154 529 Size: 35 1555 35 4802 Fragments arranged by size: 4802 1555 35 35 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2695 5434 809 Size: 507 1379 2739 1802 Fragments arranged by size: 2739 1802 1379 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2914 3573 3874 Size: 54 1425 50 275 18 1084 659 301 Cuts at: 3874 4763 6392 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1084 889 659 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2238 2265 2880 4357 1353 Size: 873 12 27 615 1477 3423 Fragments arranged by size: 3423 1477 873 615 27 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3053 3410 4490 5087 5874 6142 1239 Size: 393 1421 357 1080 597 787 268 1524 Fragments arranged by size: 1524 1421 1080 787 597 393 357 268 EcoNI CCTnn'n_nnAGG Cuts at: 2163 2933 2163 Size: 770 5657 EcoO109I rG'GnC_Cy Cuts at: 2031 2039 2482 2658 3076 3186 2031 Size: 8 443 176 418 110 5272 Fragments arranged by size: 5272 443 418 176 110 8 EcoRI G'AATT_C Cuts at: 2972 3736 4114 2972 Size: 764 378 5285 Fragments arranged by size: 5285 764 378 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2380 2464 5574 1678 Size: 702 84 3110 2531 Fragments arranged by size: 3110 2531 702 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2699 2731 3228 Size: 32 135 32 575 32 520 32 497 Cuts at: 3228 3260 3420 3452 5068 5100 5142 5174 1373 Size: 32 160 32 1616 32 42 32 2626 Fragments arranged by size: 2626 1616 575 520 497 160 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 748 Size: 1702 4725 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KasI G'GCGC_C Cuts at: 2460 2460 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5541 5725 1305 Size: 4236 184 2007 Fragments arranged by size: 4236 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3575 10 Size: 54 1425 343 1743 2862 Fragments arranged by size: 2862 1743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4385 4744 4903 518 Size: 648 3219 359 159 2042 Fragments arranged by size: 3219 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2317 2332 3091 3109 3474 3843 4520 5668 Size: 711 15 759 18 365 369 677 1148 Cuts at: 5668 5913 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 759 711 677 369 365 245 18 15 NaeI GCC'GGC Cuts at: 2474 2914 2474 Size: 440 5987 NarI GG'CG_CC Cuts at: 2461 2461 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 2664 3126 3171 513 Size: 552 1599 462 45 3769 Fragments arranged by size: 3769 1599 552 462 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NgoMIV G'CCGG_C Cuts at: 2472 2912 2472 Size: 440 5987 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2945 3232 3673 3785 5330 1132 Size: 97 1716 287 441 112 1545 2229 Fragments arranged by size: 2229 1716 1545 441 287 112 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 3228 5326 3228 Size: 2098 4329 PflMI CCAn_nnn'nTGG Cuts at: 1108 2159 1108 Size: 1051 5376 PfoI T'CCnGG_A Cuts at: 2050 3068 2050 Size: 1018 5409 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PmlI CAC'GTG Cuts at: 3163 3163 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2031 3186 2031 Size: 1155 5272 PshAI GACnn'nnGTC Cuts at: 1613 2131 1613 Size: 518 5909 PsiI TTA'TAA Cuts at: 3153 3997 3153 Size: 844 5583 PspOMI G'GGCC_C Cuts at: 2657 2657 Size: 6427 PstI C_TGCA'G Cuts at: 830 3779 830 Size: 2949 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2317 3091 2317 Size: 774 5653 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 3620 4675 6387 3620 Size: 1055 1712 3660 Fragments arranged by size: 3660 1712 1055 SexAI A'CCwGG_T Cuts at: 3216 3240 3216 Size: 24 6403 SfcI C'TryA_G Cuts at: 826 1045 3500 3775 4910 5591 5782 826 Size: 219 2455 275 1135 681 191 1471 Fragments arranged by size: 2455 1471 1135 681 275 219 191 SfoI GGC'GCC Cuts at: 2462 2462 Size: 6427 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 2573 3754 1872 Size: 701 1181 4545 Fragments arranged by size: 4545 1181 701 SmlI C'TyrA_G Cuts at: 820 1017 1943 2077 2129 4531 5432 5694 820 Size: 197 926 134 52 2402 901 262 1553 Fragments arranged by size: 2402 1553 926 901 262 197 134 52 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 2899 1517 Size: 1382 5045 StyI C'CwwG_G Cuts at: 513 1065 2664 3126 3171 3309 3866 513 Size: 552 1599 462 45 138 557 3074 Fragments arranged by size: 3074 1599 557 552 462 138 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3540 3674 4454 4471 4624 4809 5254 Size: 1255 1960 134 780 17 153 185 445 Cuts at: 5254 325 Size: 1498 Fragments arranged by size: 1960 1498 1255 780 445 185 153 134 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2732 3618 4673 Size: 274 80 33 51 1443 755 886 1055 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1055 886 755 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 2029 3242 3254 1316 Size: 713 1213 12 4489 Fragments arranged by size: 4489 1213 713 12 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3178 3076 Size: 102 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 2571 3752 1870 Size: 701 1181 4545 Fragments arranged by size: 4545 1181 701 XmnI GAAnn'nnTTC Cuts at: 2976 4556 2976 Size: 1580 4847 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI