(Circular) (Six-base) MAPSORT of: pFN21AB8699.seq Check: 4967 from: 1 to: 6328 pFN21AB8699 6328 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 2111 3649 2111 Size: 1538 4790 AclI AA'CG_TT Cuts at: 1563 3347 4455 4828 1563 Size: 1784 1108 373 3063 Fragments arranged by size: 3063 1784 1108 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 2213 4391 5775 6043 1632 Size: 517 64 2178 1384 268 1917 Fragments arranged by size: 2178 1917 1384 517 268 64 AflII C'TTAA_G Cuts at: 820 1017 2603 820 Size: 197 1586 4545 Fragments arranged by size: 4545 1586 197 AflIII A'CryG_T Cuts at: 1203 3017 5227 6204 1203 Size: 1814 2210 977 1327 Fragments arranged by size: 2210 1814 1327 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6328 AlwNI CAG_nnn'CTG Cuts at: 3530 4071 5643 3530 Size: 541 1572 4215 Fragments arranged by size: 4215 1572 541 ApaLI G'TGCA_C Cuts at: 2351 4385 5541 2351 Size: 2034 1156 3138 Fragments arranged by size: 3138 2034 1156 ApoI r'AATT_y Cuts at: 1493 2730 3211 3377 3455 3560 3637 3863 4015 Size: 1237 481 166 78 105 77 226 152 Cuts at: 4015 6124 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1237 481 226 166 152 105 78 77 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2078 3653 1870 Size: 73 135 1575 4545 Fragments arranged by size: 4545 1575 135 73 BamHI G'GATC_C Cuts at: 3658 4025 3658 Size: 367 5961 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2225 2294 2713 2860 Size: 325 217 627 324 114 69 419 147 Cuts at: 2860 3649 5104 618 Size: 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 419 325 324 217 147 114 69 BanII G_rGCy'C Cuts at: 1391 2079 2543 3647 1391 Size: 688 464 1104 4072 Fragments arranged by size: 4072 1104 688 464 BbeI G_GCGC'C Cuts at: 2229 2864 2229 Size: 635 5693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2356 3235 3609 928 Size: 971 457 879 374 3647 Fragments arranged by size: 3647 971 879 457 374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4519 4553 1903 Size: 34 2582 34 3678 Fragments arranged by size: 3678 2582 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BfrBI ATG'CAT Cuts at: 2747 2747 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 4939 136 Size: 107 122 71 631 605 616 2651 1525 Fragments arranged by size: 2651 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 3745 3745 Size: 6328 Bme1580I G_kGCm'C Cuts at: 1790 2355 2485 3025 4389 5545 1790 Size: 565 130 540 1364 1156 2573 Fragments arranged by size: 2573 1364 1156 565 540 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 2704 4059 Size: 416 53 83 399 24 849 438 1355 Cuts at: 4059 5017 442 Size: 958 1753 Fragments arranged by size: 1753 1355 958 849 438 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3152 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2493 3462 4988 1239 Size: 854 400 969 1526 2579 Fragments arranged by size: 2579 1526 969 854 400 Bpu10I CC'TnA_GC Cuts at: 2529 2529 Size: 6328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4453 5318 5616 4453 Size: 865 298 5165 Fragments arranged by size: 5165 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4991 882 Size: 617 3492 2219 Fragments arranged by size: 3492 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4119 493 Size: 711 2915 2702 Fragments arranged by size: 2915 2702 711 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 2861 4135 4517 Size: 53 83 186 936 693 635 1274 382 Cuts at: 4517 5152 275 Size: 635 1451 Fragments arranged by size: 1451 1274 936 693 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4761 5159 5433 5580 5991 4761 Size: 398 274 147 411 5098 Fragments arranged by size: 5098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 1178 Size: 30 165 30 834 30 5239 Fragments arranged by size: 5239 834 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2380 2395 3237 3240 3294 1186 Size: 1014 180 15 842 3 54 4220 Fragments arranged by size: 4220 1014 842 180 54 15 3 BseYI C'CCAG_C Cuts at: 2048 2709 5531 6027 2048 Size: 661 2822 496 2349 Fragments arranged by size: 2822 2349 661 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 1163 Size: 573 492 5263 Fragments arranged by size: 5263 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 4539 5567 1540 Size: 275 186 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2355 4389 4474 5545 2355 Size: 2034 85 1071 3138 Fragments arranged by size: 3138 2034 1071 85 BsmI GAATG_Cn' Cuts at: 3839 3932 3839 Size: 93 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2355 2485 2543 3025 3647 4389 Size: 399 289 276 130 58 482 622 742 Cuts at: 4389 4474 5545 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 622 482 399 289 276 130 85 58 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 2920 4217 5947 2920 Size: 1297 1730 3301 Fragments arranged by size: 3301 1730 1297 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 3683 844 Size: 1232 1607 3489 Fragments arranged by size: 3489 1607 1232 BsrBI CCG'CTC Cuts at: 1394 1823 2025 4215 1394 Size: 429 202 2190 3507 Fragments arranged by size: 3507 2190 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 4823 4997 65 Size: 1183 3575 174 1396 Fragments arranged by size: 3575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2181 2291 4972 5159 1700 Size: 110 371 110 2681 187 2869 Fragments arranged by size: 2869 2681 371 187 110 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 2354 4388 5400 2354 Size: 2034 1012 3282 Fragments arranged by size: 3282 2034 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 3157 3226 5132 3157 Size: 69 1906 4353 Fragments arranged by size: 4353 1906 69 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstYI r'GATC_y Cuts at: 1972 2019 3658 4025 4410 4427 5879 5965 5977 Size: 47 1639 367 385 17 1452 86 12 Cuts at: 5977 6323 1972 Size: 346 1977 Fragments arranged by size: 1977 1639 1452 385 367 346 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 3385 3385 Size: 6328 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5776 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 2027 2977 3844 4638 4658 2027 Size: 950 867 794 20 3697 Fragments arranged by size: 3697 950 867 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3352 3632 3352 Size: 280 6048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 3775 4664 Size: 54 1425 50 275 18 459 1486 889 Cuts at: 4664 6293 8 Size: 1629 43 Fragments arranged by size: 1629 1486 1425 889 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3775 1537 Size: 275 1963 4090 Fragments arranged by size: 4090 1963 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2943 3055 3121 3138 4258 1353 Size: 1590 112 66 17 1120 3423 Fragments arranged by size: 3423 1590 1120 112 66 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2564 4915 5289 5435 387 Size: 1064 138 975 2351 374 146 1280 Fragments arranged by size: 2351 1280 1064 975 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2149 2213 2493 3462 4391 4988 Size: 393 461 56 64 280 969 929 597 Cuts at: 4988 5775 6043 1239 Size: 787 268 1524 Fragments arranged by size: 1524 969 929 787 597 461 393 280 268 64 56 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 6328 EcoRI G'AATT_C Cuts at: 2730 3637 4015 2730 Size: 907 378 5043 Fragments arranged by size: 5043 907 378 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 2229 2864 5475 1678 Size: 551 635 2611 2531 Fragments arranged by size: 2611 2531 635 551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2572 2604 2966 2998 4969 Size: 32 135 32 1000 32 362 32 1971 Cuts at: 4969 5001 5043 5075 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1971 1000 362 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3672 3918 669 Size: 988 281 1734 246 3079 Fragments arranged by size: 3079 1734 988 281 246 HindIII A'AGCT_T Cuts at: 748 2148 2600 2836 3446 3470 748 Size: 1400 452 236 610 24 3606 Fragments arranged by size: 3606 1400 610 452 236 24 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KasI G'GCGC_C Cuts at: 2225 2860 2225 Size: 635 5693 KpnI G_GTAC'C Cuts at: 2115 3653 2115 Size: 1538 4790 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 3017 6204 3017 Size: 3187 3141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5442 5626 1305 Size: 4137 184 2007 Fragments arranged by size: 4137 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4506 Fragments arranged by size: 4506 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3138 4286 4645 4804 518 Size: 648 1972 1148 359 159 2042 Fragments arranged by size: 2042 1972 1148 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2242 3744 4421 5569 5814 1606 Size: 585 51 1502 677 1148 245 2120 Fragments arranged by size: 2120 1502 1148 677 585 245 51 NaeI GCC'GGC Cuts at: 2293 2293 Size: 6328 NarI GG'CG_CC Cuts at: 2226 2861 2226 Size: 635 5693 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5776 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NgoMIV G'CCGG_C Cuts at: 2291 2291 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NsiI A_TGCA'T Cuts at: 2749 2749 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 2751 3686 5231 1132 Size: 97 1522 935 1545 2229 Fragments arranged by size: 2229 1545 1522 935 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6328 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3444 3476 4397 4429 5910 5942 3444 Size: 32 921 32 1481 32 3830 Fragments arranged by size: 3830 1481 921 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PstI C_TGCA'G Cuts at: 830 3680 830 Size: 2850 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 2191 2191 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SexAI A'CCwGG_T Cuts at: 3090 3090 Size: 6328 SfcI C'TryA_G Cuts at: 826 1045 2186 3676 4811 5492 5683 826 Size: 219 1141 1490 1135 681 191 1471 Fragments arranged by size: 1490 1471 1141 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2288 2288 Size: 6328 SfoI GGC'GCC Cuts at: 2227 2862 2227 Size: 635 5693 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 2080 3655 1872 Size: 208 1575 4545 Fragments arranged by size: 4545 1575 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 4432 5333 5595 820 Size: 197 926 660 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 660 262 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3686 1132 Size: 2554 3774 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6328 StyI C'CwwG_G Cuts at: 513 1065 3767 513 Size: 552 2702 3074 Fragments arranged by size: 3074 2702 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 4355 4372 4525 4710 5155 325 Size: 1255 1121 1654 17 153 185 445 1498 Fragments arranged by size: 1654 1498 1255 1121 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4574 6286 96 Size: 274 80 33 51 1443 2597 1712 138 Fragments arranged by size: 2597 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 6288 6320 2237 Size: 32 4019 32 2245 Fragments arranged by size: 4019 2245 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 2683 3664 2683 Size: 981 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 2078 3653 1870 Size: 208 1575 4545 Fragments arranged by size: 4545 1575 208 XmnI GAAnn'nnTTC Cuts at: 3459 4457 3459 Size: 998 5330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI XcmI