(Circular) (Six-base) MAPSORT of: pFN21AB8698.seq Check: 4787 from: 1 to: 6460 pFN21AB8698 6460 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3803 1937 Size: 1866 4594 Acc65I G'GTAC_C Cuts at: 3616 3781 3616 Size: 165 6295 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3263 3382 4523 5907 6175 1632 Size: 1631 119 1141 1384 268 1917 Fragments arranged by size: 1917 1631 1384 1141 268 119 AflII C'TTAA_G Cuts at: 820 1017 2119 820 Size: 197 1102 5161 Fragments arranged by size: 5161 1102 197 AflIII A'CryG_T Cuts at: 1203 2417 3253 5359 6336 1203 Size: 1214 836 2106 977 1327 Fragments arranged by size: 2106 1327 1214 977 836 AgeI A'CCGG_T Cuts at: 3586 5291 3586 Size: 1705 4755 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 2018 1166 Size: 852 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AlwNI CAG_nnn'CTG Cuts at: 3052 4203 5775 3052 Size: 1151 1572 3737 Fragments arranged by size: 3737 1572 1151 ApaLI G'TGCA_C Cuts at: 4517 5673 4517 Size: 1156 5304 ApoI r'AATT_y Cuts at: 1493 3503 3769 3995 4147 6256 1493 Size: 2010 266 226 152 2109 1697 Fragments arranged by size: 2109 2010 1697 266 226 152 AseI AT'TA_AT Cuts at: 160 1032 2924 5014 160 Size: 872 1892 2090 1606 Fragments arranged by size: 2090 1892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 3616 3781 5236 618 Size: 325 217 627 758 1071 165 1455 1842 Fragments arranged by size: 1842 1455 1071 758 627 325 217 165 BanII G_rGCy'C Cuts at: 1391 3779 1391 Size: 2388 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5489 BbvCI CC'TCA_GC Cuts at: 2916 3006 2916 Size: 90 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4352 5562 4352 Size: 1210 5250 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5071 136 Size: 107 122 71 631 605 3399 1525 Fragments arranged by size: 3399 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3633 3877 3633 Size: 244 6216 Bme1580I G_kGCm'C Cuts at: 1790 2682 4521 5677 1790 Size: 892 1839 1156 2573 Fragments arranged by size: 2573 1839 1156 892 BmgBI CAC'GTC Cuts at: 1318 3254 1318 Size: 1936 4524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2678 4191 5149 Size: 416 53 83 399 24 1261 1513 958 Cuts at: 5149 442 Size: 1753 Fragments arranged by size: 1753 1513 1261 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 5120 1239 Size: 1023 2858 2579 Fragments arranged by size: 2858 2579 1023 Bpu10I CC'TnA_GC Cuts at: 2916 3006 2916 Size: 90 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3594 4585 5450 5748 3594 Size: 991 865 298 4306 Fragments arranged by size: 4306 991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 2238 3486 5123 882 Size: 617 524 215 1248 1637 2219 Fragments arranged by size: 2219 1637 1248 617 524 215 BsaAI yAC'GTr Cuts at: 493 1204 2420 4251 493 Size: 711 1216 1831 2702 Fragments arranged by size: 2702 1831 1216 711 BsaBI GATnn'nnATC Cuts at: 3237 6454 3237 Size: 3217 3243 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4267 4649 5284 275 Size: 53 83 186 936 2734 382 635 1451 Fragments arranged by size: 2734 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3586 4893 5291 5565 5712 6123 3586 Size: 1307 398 274 147 411 3923 Fragments arranged by size: 3923 1307 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6235 Fragments arranged by size: 6235 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2883 3063 3734 1186 Size: 1697 180 671 3912 Fragments arranged by size: 3912 1697 671 180 BseYI C'CCAG_C Cuts at: 3018 3063 5663 6159 3018 Size: 45 2600 496 3319 Fragments arranged by size: 3319 2600 496 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2715 3053 1163 Size: 573 979 338 4570 Fragments arranged by size: 4570 979 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4521 4606 5677 4521 Size: 85 1071 5304 Fragments arranged by size: 5304 1071 85 BsmI GAATG_Cn' Cuts at: 3971 4064 3971 Size: 93 6367 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 3779 4521 4606 5677 1391 Size: 399 892 1097 742 85 1071 2174 Fragments arranged by size: 2174 1097 1071 892 742 399 85 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 4349 6079 4349 Size: 1730 4730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3815 844 Size: 2971 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3113 4347 1394 Size: 429 1290 1234 3507 Fragments arranged by size: 3507 1290 1234 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2533 2661 2724 2779 4955 5129 65 Size: 1183 1285 128 63 55 2176 174 1396 Fragments arranged by size: 2176 1396 1285 1183 174 128 63 55 BsrFI r'CCGG_y Cuts at: 1700 1810 3586 5104 5291 1700 Size: 110 1776 1518 187 2869 Fragments arranged by size: 2869 1776 1518 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 3052 3184 794 Size: 2258 132 4070 Fragments arranged by size: 4070 2258 132 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 2597 3589 1184 Size: 1413 992 4055 Fragments arranged by size: 4055 1413 992 BstXI CCAn_nnnn'nTGG Cuts at: 2020 2715 3107 2020 Size: 695 392 5373 Fragments arranged by size: 5373 695 392 BstYI r'GATC_y Cuts at: 1972 2164 2423 3519 3790 4157 4542 4559 6011 Size: 192 259 1096 271 367 385 17 1452 Cuts at: 6011 6097 6109 6455 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1096 385 367 346 271 259 192 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5908 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 2619 2653 2698 3124 3418 3976 4770 4790 2619 Size: 34 45 426 294 558 794 20 4289 Fragments arranged by size: 4289 794 558 426 294 45 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2922 2957 529 Size: 35 2358 35 4032 Fragments arranged by size: 4032 2358 35 35 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DraIII CAC_nnn'GTG Cuts at: 3031 3031 Size: 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425 Size: 54 1425 50 275 18 2077 889 1629 Cuts at: 6425 8 Size: 43 Fragments arranged by size: 2077 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3401 3711 4390 1353 Size: 2048 310 679 3423 Fragments arranged by size: 3423 2048 679 310 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 3263 3382 4523 5120 5907 6175 Size: 393 630 1001 119 1141 597 787 268 Cuts at: 6175 1239 Size: 1524 Fragments arranged by size: 1524 1141 1001 787 630 597 393 268 119 EcoO109I rG'GnC_Cy Cuts at: 3002 3002 Size: 6460 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2469 2469 Size: 6460 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 5607 1678 Size: 3929 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5101 5133 5175 5207 1373 Size: 32 135 32 3529 32 42 32 2626 Fragments arranged by size: 3529 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2327 2366 3804 4050 669 Size: 988 281 389 39 1438 246 3079 Fragments arranged by size: 3079 1438 988 389 281 246 39 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KpnI G_GTAC'C Cuts at: 3620 3785 3620 Size: 165 6295 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5574 5758 1305 Size: 4269 184 2007 Fragments arranged by size: 4269 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4638 Fragments arranged by size: 4638 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 3423 4418 4777 4936 518 Size: 648 852 1405 995 359 159 2042 Fragments arranged by size: 2042 1405 995 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2240 2609 3052 3876 4553 5701 5946 1606 Size: 634 369 443 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 634 443 369 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5908 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 2176 3818 5363 1132 Size: 97 947 1642 1545 2229 Fragments arranged by size: 2229 1642 1545 947 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 2198 2360 1108 Size: 1090 162 5208 Fragments arranged by size: 5208 1090 162 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PmlI CAC'GTG Cuts at: 2420 2420 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4529 4561 6042 6074 4529 Size: 32 1481 32 4915 Fragments arranged by size: 4915 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3002 3002 Size: 6460 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 3263 4030 3263 Size: 767 5693 PstI C_TGCA'G Cuts at: 830 2614 3057 3812 830 Size: 1784 443 755 3478 Fragments arranged by size: 3478 1784 755 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 2240 2609 3052 2240 Size: 369 443 5648 Fragments arranged by size: 5648 443 369 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 3325 4708 6420 3325 Size: 1383 1712 3365 Fragments arranged by size: 3365 1712 1383 SfcI C'TryA_G Cuts at: 826 1045 2610 2625 3053 3808 4943 5624 5815 Size: 219 1565 15 428 755 1135 681 191 Cuts at: 5815 826 Size: 1471 Fragments arranged by size: 1565 1471 1135 755 681 428 219 191 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2119 3609 4564 5465 5727 820 Size: 197 926 176 1490 955 901 262 1553 Fragments arranged by size: 1553 1490 955 926 901 262 197 176 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 2830 4384 5 Size: 47 2778 1554 2081 Fragments arranged by size: 2778 2081 1554 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6460 StyI C'CwwG_G Cuts at: 513 1065 2215 2766 3899 513 Size: 552 1150 551 1133 3074 Fragments arranged by size: 3074 1150 1133 552 551 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4487 4504 4657 4842 5287 325 Size: 1255 2907 17 153 185 445 1498 Fragments arranged by size: 2907 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2059 3323 4706 Size: 274 80 33 51 1443 82 1264 1383 Cuts at: 4706 6418 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1383 1264 274 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 6420 6452 2213 Size: 32 4175 32 2221 Fragments arranged by size: 4175 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 2773 Size: 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 4589 4589 Size: 6460 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI