(Circular) (Six-base) MAPSORT of: pFN21AB8697.seq Check: 1053 from: 1 to: 6343 pFN21AB8697 6343 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2691 2691 Size: 6343 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4153 278 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3686 1937 Size: 1749 4594 Acc65I G'GTAC_C Cuts at: 3664 3664 Size: 6343 AclI AA'CG_TT Cuts at: 1563 4470 4843 1563 Size: 2907 373 3063 Fragments arranged by size: 3063 2907 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 4406 5790 6058 1632 Size: 1541 1233 1384 268 1917 Fragments arranged by size: 1917 1541 1384 1233 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6146 AflIII A'CryG_T Cuts at: 1203 5242 6219 1203 Size: 4039 977 1327 Fragments arranged by size: 4039 1327 977 AgeI A'CCGG_T Cuts at: 3300 5174 3300 Size: 1874 4469 AhdI GACnn_n'nnGTC Cuts at: 5072 5072 Size: 6343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3180 1184 Size: 1996 4347 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3191 3223 3191 Size: 32 6311 AlwNI CAG_nnn'CTG Cuts at: 3010 3466 4086 5658 3010 Size: 456 620 1572 3695 Fragments arranged by size: 3695 1572 620 456 ApaI G_GGCC'C Cuts at: 2206 2288 2794 2206 Size: 82 506 5755 Fragments arranged by size: 5755 506 82 ApaLI G'TGCA_C Cuts at: 4400 5556 4400 Size: 1156 5187 ApoI r'AATT_y Cuts at: 1493 2617 3652 3878 4030 6139 1493 Size: 1124 1035 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 1035 226 152 AseI AT'TA_AT Cuts at: 160 1032 4897 160 Size: 872 3865 1606 Fragments arranged by size: 3865 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2793 3668 1870 Size: 73 141 709 875 4545 Fragments arranged by size: 4545 875 709 141 73 BamHI G'GATC_C Cuts at: 2419 3673 4040 2419 Size: 1254 367 4722 Fragments arranged by size: 4722 1254 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3066 3664 5119 618 Size: 325 217 627 1279 598 1455 1842 Fragments arranged by size: 1842 1455 1279 627 598 325 217 BanII G_rGCy'C Cuts at: 1391 2095 2179 2206 2288 2794 3488 3535 3627 Size: 704 84 27 82 506 694 47 92 Cuts at: 3627 3662 1391 Size: 35 4072 Fragments arranged by size: 4072 704 694 506 92 84 82 47 35 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2974 928 Size: 971 351 724 4297 Fragments arranged by size: 4297 971 724 351 BbvCI CC'TCA_GC Cuts at: 3345 3345 Size: 6343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4534 4568 1903 Size: 34 2597 34 3678 Fragments arranged by size: 3678 2597 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4235 5445 4235 Size: 1210 5133 BclI T'GATC_A Cuts at: 1548 1548 Size: 6343 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2189 2711 3523 Size: 107 122 71 631 605 517 522 812 Cuts at: 3523 4954 136 Size: 1431 1525 Fragments arranged by size: 1525 1431 812 631 605 522 517 122 107 71 BglII A'GATC_T Cuts at: 6338 6338 Size: 6343 BlpI GC'TnA_GC Cuts at: 2228 3760 2228 Size: 1532 4811 Bme1580I G_kGCm'C Cuts at: 1790 2206 2288 2305 2794 3424 4404 5560 1790 Size: 416 82 17 489 630 980 1156 2573 Fragments arranged by size: 2573 1156 980 630 489 416 82 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4074 5032 Size: 416 53 83 399 24 2204 453 958 Cuts at: 5032 442 Size: 1753 Fragments arranged by size: 2204 1753 958 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6343 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2842 2905 3646 2842 Size: 63 741 5539 Fragments arranged by size: 5539 741 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 2624 3011 3299 3395 3425 5003 1239 Size: 1128 257 387 288 96 30 1578 2579 Fragments arranged by size: 2579 1578 1128 387 288 257 96 30 Bpu10I CC'TnA_GC Cuts at: 3345 3345 Size: 6343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2163 2747 2753 2915 3356 3519 3603 4468 5333 Size: 584 6 162 441 163 84 865 865 Cuts at: 5333 5631 2163 Size: 298 2875 Fragments arranged by size: 2875 865 865 584 441 298 163 162 84 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2454 2460 3137 5006 882 Size: 617 955 6 677 1869 2219 Fragments arranged by size: 2219 1869 955 677 617 6 BsaAI yAC'GTr Cuts at: 493 1204 4134 493 Size: 711 2930 2702 Fragments arranged by size: 2930 2702 711 BsaBI GATnn'nnATC Cuts at: 6337 6337 Size: 6343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4150 4532 5167 275 Size: 53 83 186 936 2617 382 635 1451 Fragments arranged by size: 2617 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2071 2613 3300 4776 5174 5448 5595 6006 2071 Size: 542 687 1476 398 274 147 411 2408 Fragments arranged by size: 2408 1476 687 542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 2747 2777 3398 Size: 30 165 30 1104 30 210 30 621 Cuts at: 3398 3428 1178 Size: 30 4093 Fragments arranged by size: 4093 1104 621 210 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 2237 2576 2621 2813 2816 2819 2978 Size: 962 89 339 45 192 3 3 159 Cuts at: 2978 3203 3362 3526 1186 Size: 225 159 164 4003 Fragments arranged by size: 4003 962 339 225 192 164 159 159 89 45 3 3 BseYI C'CCAG_C Cuts at: 2105 3542 5546 6042 2105 Size: 1437 2004 496 2406 Fragments arranged by size: 2406 2004 1437 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 1163 Size: 573 1068 4702 Fragments arranged by size: 4702 1068 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3793 4554 5582 1540 Size: 275 186 1792 761 1028 2301 Fragments arranged by size: 2301 1792 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2258 2613 2697 3586 3593 4404 4489 5560 2258 Size: 355 84 889 7 811 85 1071 3041 Fragments arranged by size: 3041 1071 889 811 355 85 84 7 BsmI GAATG_Cn' Cuts at: 3854 3947 3854 Size: 93 6250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2179 2206 2258 2288 2305 2613 Size: 399 305 84 27 52 30 17 308 Cuts at: 2613 2697 2794 3424 3488 3535 3586 3593 3627 Size: 84 97 630 64 47 51 7 34 Cuts at: 3627 3662 4404 4489 5560 1391 Size: 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 630 399 308 305 97 85 84 84 64 52 51 47 35 34 30 27 17 7 BspEI T'CCGG_A Cuts at: 6006 6006 Size: 6343 BspHI T'CATG_A Cuts at: 4232 5962 4232 Size: 1730 4613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 3376 3698 844 Size: 1847 685 322 3489 Fragments arranged by size: 3489 1847 685 322 BsrBI CCG'CTC Cuts at: 1394 1823 2101 2763 3064 4230 1394 Size: 429 278 662 301 1166 3507 Fragments arranged by size: 3507 1166 662 429 301 278 BsrDI GCAATG_nn' Cuts at: 65 1248 3445 4838 5012 65 Size: 1183 2197 1393 174 1396 Fragments arranged by size: 2197 1396 1393 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2314 3300 4987 5174 1700 Size: 110 504 986 1687 187 2869 Fragments arranged by size: 2869 1687 986 504 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6343 BssSI C'ACGA_G Cuts at: 4403 5415 4403 Size: 1012 5331 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6343 BstBI TT'CG_AA Cuts at: 5147 5147 Size: 6343 BstEII G'GTnAC_C Cuts at: 1184 2821 1184 Size: 1637 4706 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2506 Size: 6343 BstYI r'GATC_y Cuts at: 1972 2419 2436 3673 4040 4425 4442 5894 5980 Size: 447 17 1237 367 385 17 1452 86 Cuts at: 5980 5992 6338 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1237 447 385 367 346 86 17 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5791 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5604 Fragments arranged by size: 5604 683 56 BtsI GCAGTG_nn' Cuts at: 3422 3859 4653 4673 3422 Size: 437 794 20 5092 Fragments arranged by size: 5092 794 437 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6343 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6308 DraI TTT'AAA Cuts at: 3647 3647 Size: 6343 DraIII CAC_nnn'GTG Cuts at: 2309 2309 Size: 6343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5350 809 Size: 507 4034 1802 Fragments arranged by size: 4034 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3478 3790 4679 Size: 54 1425 50 275 18 1648 312 889 Cuts at: 4679 6308 8 Size: 1629 43 Fragments arranged by size: 1648 1629 1425 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3790 1537 Size: 275 1978 4090 Fragments arranged by size: 4090 1978 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2427 3054 4273 1353 Size: 1074 627 1219 3423 Fragments arranged by size: 3423 1219 1074 627 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3622 4930 5304 5450 387 Size: 1064 138 2033 1308 374 146 1280 Fragments arranged by size: 2033 1308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2367 2624 3011 3173 3299 3395 3425 Size: 393 735 257 387 162 126 96 30 Cuts at: 3425 4406 5003 5790 6058 1239 Size: 981 597 787 268 1524 Fragments arranged by size: 1524 981 787 735 597 393 387 268 257 162 126 96 30 EcoNI CCTnn'n_nnAGG Cuts at: 3095 3634 3095 Size: 539 5804 EcoO109I rG'GnC_Cy Cuts at: 2081 2138 2202 2203 2284 2285 2995 3328 3379 Size: 57 64 1 81 1 710 333 51 Cuts at: 3379 3463 3524 2081 Size: 84 61 4900 Fragments arranged by size: 4900 710 333 84 81 64 61 57 51 1 1 EcoRI G'AATT_C Cuts at: 2617 3652 4030 2617 Size: 1035 378 4930 Fragments arranged by size: 4930 1035 378 FspI TGC'GCA Cuts at: 4849 4849 Size: 6343 HaeII r_GCGC'y Cuts at: 1678 3569 5490 1678 Size: 1891 1921 2531 Fragments arranged by size: 2531 1921 1891 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3601 3633 4984 5016 5058 Size: 32 135 32 2029 32 1351 32 42 Cuts at: 5058 5090 1373 Size: 32 2626 Fragments arranged by size: 2626 2029 1351 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3687 3933 669 Size: 988 281 1749 246 3079 Fragments arranged by size: 3079 1749 988 281 246 HindIII A'AGCT_T Cuts at: 748 2723 748 Size: 1975 4368 HpaI GTT'AAC Cuts at: 3933 3933 Size: 6343 KpnI G_GTAC'C Cuts at: 3668 3668 Size: 6343 MfeI C'AATT_G Cuts at: 3942 3942 Size: 6343 MluI A'CGCG_T Cuts at: 6219 6219 Size: 6343 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 3421 5457 5641 1305 Size: 1132 984 2036 184 2007 Fragments arranged by size: 2036 2007 1132 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3480 10 Size: 54 1425 343 1648 2873 Fragments arranged by size: 2873 1648 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4301 4660 4819 518 Size: 648 3135 359 159 2042 Fragments arranged by size: 3135 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2662 2752 2788 2860 3010 3073 3759 4436 Size: 1056 90 36 72 150 63 686 677 Cuts at: 4436 5584 5829 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1056 686 677 245 150 90 72 63 36 NaeI GCC'GGC Cuts at: 2316 2316 Size: 6343 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5791 NdeI CA'TA_TG Cuts at: 387 387 Size: 6343 NgoMIV G'CCGG_C Cuts at: 2314 2314 Size: 6343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6343 NotI GC'GGCC_GC Cuts at: 3790 3790 Size: 6343 NspI r_CATG'y Cuts at: 1132 1229 3701 5246 1132 Size: 97 2472 1545 2229 Fragments arranged by size: 2472 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3552 1348 Size: 2204 4139 PciI A'CATG_T Cuts at: 5242 5242 Size: 6343 PflMI CCAn_nnn'nTGG Cuts at: 1108 3403 1108 Size: 2295 4048 PfoI T'CCnGG_A Cuts at: 2342 3360 2342 Size: 1018 5325 PmeI GTTT'AAAC Cuts at: 3647 3647 Size: 6343 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4412 4444 5925 5957 4412 Size: 32 1481 32 4798 Fragments arranged by size: 4798 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2138 2995 3463 2081 Size: 57 857 468 4961 Fragments arranged by size: 4961 857 468 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6343 PsiI TTA'TAA Cuts at: 3913 3913 Size: 6343 PspOMI G'GGCC_C Cuts at: 2202 2284 2790 2202 Size: 82 506 5755 Fragments arranged by size: 5755 506 82 PstI C_TGCA'G Cuts at: 830 2125 2751 2865 3387 3429 3695 830 Size: 1295 626 114 522 42 266 3478 Fragments arranged by size: 3478 1295 626 522 266 114 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6343 PvuII CAG'CTG Cuts at: 2662 2860 3010 2662 Size: 198 150 5995 Fragments arranged by size: 5995 198 150 SalI G'TCGA_C Cuts at: 1936 3685 1936 Size: 1749 4594 SapI GCTCTTCn'nnn_ Cuts at: 3054 3054 Size: 6343 SbfI CC_TGCA'GG Cuts at: 3387 3695 3387 Size: 308 6035 ScaI AGT'ACT Cuts at: 4591 6303 4591 Size: 1712 4631 SfcI C'TryA_G Cuts at: 826 1045 2121 2642 2747 2861 3383 3425 3691 Size: 219 1076 521 105 114 522 42 266 Cuts at: 3691 4826 5507 5698 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1076 681 522 521 266 219 191 114 105 42 SfiI GGCCn_nnn'nGGCC Cuts at: 2711 3523 2711 Size: 812 5531 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6343 SmaI CCC'GGG Cuts at: 1872 2795 3670 1872 Size: 923 875 4545 Fragments arranged by size: 4545 923 875 SmlI C'TyrA_G Cuts at: 820 1017 1943 2178 2726 2768 2930 3335 3534 Size: 197 926 235 548 42 162 405 199 Cuts at: 3534 3618 4447 5348 5610 820 Size: 84 829 901 262 1553 Fragments arranged by size: 1553 926 901 829 548 405 262 235 199 197 162 84 42 SnaBI TAC'GTA Cuts at: 493 4134 493 Size: 3641 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6343 SphI G_CATG'C Cuts at: 1132 3701 1132 Size: 2569 3774 SspI AAT'ATT Cuts at: 5 52 4267 5 Size: 47 4215 2081 Fragments arranged by size: 4215 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6343 StyI C'CwwG_G Cuts at: 513 1065 2412 2499 3782 513 Size: 552 1347 87 1283 3074 Fragments arranged by size: 3074 1347 1283 552 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2478 3556 4370 4387 4540 4725 5170 Size: 1255 898 1078 814 17 153 185 445 Cuts at: 5170 325 Size: 1498 Fragments arranged by size: 1498 1255 1078 898 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4589 6301 96 Size: 274 80 33 51 1443 2612 1712 138 Fragments arranged by size: 2612 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2747 2779 3398 3430 6303 6335 2747 Size: 32 619 32 2873 32 2755 Fragments arranged by size: 2873 2755 619 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 2978 1316 Size: 1137 525 4681 Fragments arranged by size: 4681 1137 525 XbaI T'CTAG_A Cuts at: 3679 3679 Size: 6343 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6343 XmaI C'CCGG_G Cuts at: 1870 2793 3668 1870 Size: 923 875 4545 Fragments arranged by size: 4545 923 875 XmnI GAAnn'nnTTC Cuts at: 4472 4472 Size: 6343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4151 276 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI