(Circular) (Six-base) MAPSORT of: pFN21AB8695.seq Check: 9167 from: 1 to: 6865 pFN21AB8695 6865 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4675 278 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2140 4208 1937 Size: 203 2068 4594 Fragments arranged by size: 4594 2068 203 Acc65I G'GTAC_C Cuts at: 4186 4186 Size: 6865 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3032 3207 3248 4928 6312 6580 1632 Size: 1400 175 41 1680 1384 268 1917 Fragments arranged by size: 1917 1680 1400 1384 268 175 41 AflII C'TTAA_G Cuts at: 820 1017 3626 820 Size: 197 2609 4059 Fragments arranged by size: 4059 2609 197 AflIII A'CryG_T Cuts at: 1203 3677 5764 6741 1203 Size: 2474 2087 977 1327 Fragments arranged by size: 2474 2087 1327 977 AgeI A'CCGG_T Cuts at: 5696 5696 Size: 6865 AhdI GACnn_n'nnGTC Cuts at: 5594 5594 Size: 6865 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6865 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2218 1184 Size: 1034 5831 AlwNI CAG_nnn'CTG Cuts at: 2756 2789 4608 6180 2756 Size: 33 1819 1572 3441 Fragments arranged by size: 3441 1819 1572 33 ApaLI G'TGCA_C Cuts at: 4922 6078 4922 Size: 1156 5709 ApoI r'AATT_y Cuts at: 1493 2406 2600 3179 3282 3333 4174 4400 4552 Size: 913 194 579 103 51 841 226 152 Cuts at: 4552 6661 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 913 841 579 226 194 152 103 51 AseI AT'TA_AT Cuts at: 160 1032 2013 2637 4104 5419 160 Size: 872 981 624 1467 1315 1606 Fragments arranged by size: 1606 1467 1315 981 872 624 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 3093 4190 1870 Size: 73 1150 1097 4545 Fragments arranged by size: 4545 1150 1097 73 BamHI G'GATC_C Cuts at: 3307 4195 4562 3307 Size: 888 367 5610 Fragments arranged by size: 5610 888 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3458 4186 5641 618 Size: 325 217 627 1671 728 1455 1842 Fragments arranged by size: 1842 1671 1455 728 627 325 217 BanII G_rGCy'C Cuts at: 1391 4184 1391 Size: 2793 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5894 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5056 5090 1903 Size: 34 3119 34 3678 Fragments arranged by size: 3678 3119 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3796 4757 5967 3796 Size: 961 1210 4694 Fragments arranged by size: 4694 1210 961 BclI T'GATC_A Cuts at: 1548 2995 1548 Size: 1447 5418 BfrBI ATG'CAT Cuts at: 3593 3739 3593 Size: 146 6719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5476 136 Size: 107 122 71 631 605 3804 1525 Fragments arranged by size: 3804 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2744 6860 2744 Size: 4116 2749 BlpI GC'TnA_GC Cuts at: 4282 4282 Size: 6865 Bme1580I G_kGCm'C Cuts at: 1790 3797 4926 6082 1790 Size: 2007 1129 1156 2573 Fragments arranged by size: 2573 2007 1156 1129 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6865 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4596 5554 442 Size: 416 53 83 399 24 3179 958 1753 Fragments arranged by size: 3179 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 2733 3362 5525 1239 Size: 901 593 629 2163 2579 Fragments arranged by size: 2579 2163 901 629 593 Bpu10I CC'TnA_GC Cuts at: 2109 2109 Size: 6865 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4990 5855 6153 4990 Size: 865 298 5702 Fragments arranged by size: 5702 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5528 882 Size: 617 4029 2219 Fragments arranged by size: 4029 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4656 493 Size: 711 3452 2702 Fragments arranged by size: 3452 2702 711 BsaBI GATnn'nnATC Cuts at: 6859 6859 Size: 6865 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4672 5054 5689 275 Size: 53 83 186 936 3139 382 635 1451 Fragments arranged by size: 3139 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5298 5696 5970 6117 6528 5298 Size: 398 274 147 411 5635 Fragments arranged by size: 5635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2257 2287 3189 3219 1178 Size: 30 165 30 854 30 902 30 4824 Fragments arranged by size: 4824 902 854 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3041 1186 Size: 1855 5010 BseYI C'CCAG_C Cuts at: 6068 6564 6068 Size: 496 6369 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3630 1163 Size: 573 1894 4398 Fragments arranged by size: 4398 1894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4315 5076 6104 1540 Size: 275 186 2314 761 1028 2301 Fragments arranged by size: 2314 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4926 5011 6082 4926 Size: 85 1071 5709 Fragments arranged by size: 5709 1071 85 BsmI GAATG_Cn' Cuts at: 4376 4469 4376 Size: 93 6772 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3797 4184 4926 5011 6082 1391 Size: 399 2007 387 742 85 1071 2174 Fragments arranged by size: 2174 2007 1071 742 399 387 85 BspEI T'CCGG_A Cuts at: 6528 6528 Size: 6865 BspHI T'CATG_A Cuts at: 4754 6484 4754 Size: 1730 5135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4220 844 Size: 3376 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4752 1394 Size: 429 2929 3507 Fragments arranged by size: 3507 2929 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5360 5534 65 Size: 1183 4112 174 1396 Fragments arranged by size: 4112 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5509 5696 1700 Size: 110 3699 187 2869 Fragments arranged by size: 3699 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2791 2896 96 Size: 2695 105 4065 Fragments arranged by size: 4065 2695 105 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 4925 5937 4925 Size: 1012 5853 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6865 BstBI TT'CG_AA Cuts at: 5669 5669 Size: 6865 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6865 BstYI r'GATC_y Cuts at: 1972 2744 2752 3307 3641 4195 4562 4947 4964 Size: 772 8 555 334 554 367 385 17 Cuts at: 4964 6416 6502 6514 6860 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 772 555 554 385 367 346 334 86 17 12 8 Bsu36I CC'TnA_GG Cuts at: 3684 3684 Size: 6865 BtgI C'CryG_G Cuts at: 513 1065 2233 2886 513 Size: 552 1168 653 4492 Fragments arranged by size: 4492 1168 653 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6126 Fragments arranged by size: 6126 683 56 BtsI GCAGTG_nn' Cuts at: 4381 5175 5195 4381 Size: 794 20 6051 Fragments arranged by size: 6051 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2584 2619 3348 3383 529 Size: 35 2020 35 729 35 4011 Fragments arranged by size: 4011 2020 729 35 35 35 DraI TTT'AAA Cuts at: 4169 4169 Size: 6865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5872 809 Size: 507 4556 1802 Fragments arranged by size: 4556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3758 4312 5201 Size: 54 1425 50 275 18 1928 554 889 Cuts at: 5201 6830 8 Size: 1629 43 Fragments arranged by size: 1928 1629 1425 889 554 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4312 1537 Size: 275 2500 4090 Fragments arranged by size: 4090 2500 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 2724 2973 3051 3267 4795 1353 Size: 1161 210 249 78 216 1528 3423 Fragments arranged by size: 3423 1528 1161 249 216 210 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5452 5826 5972 387 Size: 1064 138 3863 374 146 1280 Fragments arranged by size: 3863 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2733 3032 3207 3248 3362 4928 Size: 393 508 593 299 175 41 114 1566 Cuts at: 4928 5525 6312 6580 1239 Size: 597 787 268 1524 Fragments arranged by size: 1566 1524 787 597 593 508 393 299 268 175 114 41 EcoO109I rG'GnC_Cy Cuts at: 3090 3090 Size: 6865 EcoRI G'AATT_C Cuts at: 3333 4174 4552 3333 Size: 841 378 5646 Fragments arranged by size: 5646 841 378 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 6012 1678 Size: 4334 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3154 3186 3189 3221 5506 Size: 32 135 32 1582 32 3 32 2285 Cuts at: 5506 5538 5580 5612 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2285 1582 135 42 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 2901 3841 748 Size: 2153 940 3772 Fragments arranged by size: 3772 2153 940 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KpnI G_GTAC'C Cuts at: 4190 4190 Size: 6865 MfeI C'AATT_G Cuts at: 3342 4464 3342 Size: 1122 5743 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3544 3563 3993 5979 6163 1305 Size: 2239 19 430 1986 184 2007 Fragments arranged by size: 2239 2007 1986 430 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 3760 10 Size: 54 1425 343 1928 3115 Fragments arranged by size: 3115 1928 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 4823 5182 5341 518 Size: 648 1137 2520 359 159 2042 Fragments arranged by size: 2520 2042 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4006 4281 4958 6106 6351 1606 Size: 2400 275 677 1148 245 2120 Fragments arranged by size: 2400 2120 1148 677 275 245 NcoI C'CATG_G Cuts at: 513 1065 2233 513 Size: 552 1168 5145 Fragments arranged by size: 5145 1168 552 NdeI CA'TA_TG Cuts at: 387 3492 387 Size: 3105 3760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6865 NotI GC'GGCC_GC Cuts at: 4312 4312 Size: 6865 NsiI A_TGCA'T Cuts at: 3595 3741 3595 Size: 146 6719 NspI r_CATG'y Cuts at: 1132 1229 3681 4223 5768 1132 Size: 97 2452 542 1545 2229 Fragments arranged by size: 2452 2229 1545 542 97 PasI CC'CwG_GG Cuts at: 1348 3790 1348 Size: 2442 4423 PciI A'CATG_T Cuts at: 3677 5764 3677 Size: 2087 4778 PflMI CCAn_nnn'nTGG Cuts at: 1108 2756 1108 Size: 1648 5217 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 6865 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4934 4966 6447 6479 4934 Size: 32 1481 32 5320 Fragments arranged by size: 5320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3090 3090 Size: 6865 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6865 PsiI TTA'TAA Cuts at: 2772 4435 2772 Size: 1663 5202 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2084 2116 2084 Size: 32 6833 PstI C_TGCA'G Cuts at: 830 3274 4011 4098 4217 830 Size: 2444 737 87 119 3478 Fragments arranged by size: 3478 2444 737 119 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 4006 4006 Size: 6865 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SapI GCTCTTCn'nnn_ Cuts at: 2973 2973 Size: 6865 SbfI CC_TGCA'GG Cuts at: 4217 4217 Size: 6865 ScaI AGT'ACT Cuts at: 2323 5113 6825 2323 Size: 2790 1712 2363 Fragments arranged by size: 2790 2363 1712 SfcI C'TryA_G Cuts at: 826 1045 2022 3270 3879 4007 4094 4213 5348 Size: 219 977 1248 609 128 87 119 1135 Cuts at: 5348 6029 6220 826 Size: 681 191 1471 Fragments arranged by size: 1471 1248 1135 977 681 609 219 191 128 119 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6865 SmaI CCC'GGG Cuts at: 1872 3095 4192 1872 Size: 1223 1097 4545 Fragments arranged by size: 4545 1223 1097 SmlI C'TyrA_G Cuts at: 820 1017 1943 3626 4969 5870 6132 820 Size: 197 926 1683 1343 901 262 1553 Fragments arranged by size: 1683 1553 1343 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4656 493 Size: 4163 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6865 SphI G_CATG'C Cuts at: 1132 4223 1132 Size: 3091 3774 SspI AAT'ATT Cuts at: 5 52 4071 4789 5 Size: 47 4019 718 2081 Fragments arranged by size: 4019 2081 718 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6865 StyI C'CwwG_G Cuts at: 513 1065 2233 2277 3498 4304 513 Size: 552 1168 44 1221 806 3074 Fragments arranged by size: 3074 1221 1168 806 552 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4892 4909 5062 5247 5692 325 Size: 1255 3312 17 153 185 445 1498 Fragments arranged by size: 3312 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2321 2791 2896 Size: 274 80 33 51 1443 344 470 105 Cuts at: 2896 5111 6823 96 Size: 2215 1712 138 Fragments arranged by size: 2215 1712 1443 470 344 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 2257 2289 3183 3215 6825 6857 2173 Size: 32 52 32 894 32 3610 32 2181 Fragments arranged by size: 3610 2181 894 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6865 XbaI T'CTAG_A Cuts at: 3175 3973 4201 3175 Size: 798 228 5839 Fragments arranged by size: 5839 798 228 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6865 XmaI C'CCGG_G Cuts at: 1870 3093 4190 1870 Size: 1223 1097 4545 Fragments arranged by size: 4545 1223 1097 XmnI GAAnn'nnTTC Cuts at: 3261 3621 4143 4994 3261 Size: 360 522 851 5132 Fragments arranged by size: 5132 851 522 360 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4673 276 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI