(Circular) (Six-base) MAPSORT of: pFN21AB8691.seq Check: 6701 from: 1 to: 6784 pFN21AB8691 6784 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4594 278 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4127 1937 Size: 2190 4594 Acc65I G'GTAC_C Cuts at: 4105 4105 Size: 6784 AclI AA'CG_TT Cuts at: 1563 4911 5284 1563 Size: 3348 373 3063 Fragments arranged by size: 3348 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3579 4019 4847 6231 6499 1632 Size: 1947 440 828 1384 268 1917 Fragments arranged by size: 1947 1917 1384 828 440 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6587 AflIII A'CryG_T Cuts at: 1203 5683 6660 1203 Size: 4480 977 1327 Fragments arranged by size: 4480 1327 977 AgeI A'CCGG_T Cuts at: 5615 5615 Size: 6784 AhdI GACnn_n'nnGTC Cuts at: 5513 5513 Size: 6784 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6784 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6784 AlwNI CAG_nnn'CTG Cuts at: 2176 2659 3131 4527 6099 2176 Size: 483 472 1396 1572 2861 Fragments arranged by size: 2861 1572 1396 483 472 ApaI G_GGCC'C Cuts at: 2207 2440 2207 Size: 233 6551 ApaLI G'TGCA_C Cuts at: 4841 5997 4841 Size: 1156 5628 ApoI r'AATT_y Cuts at: 1493 2829 4093 4319 4471 6580 1493 Size: 1336 1264 226 152 2109 1697 Fragments arranged by size: 2109 1697 1336 1264 226 152 AseI AT'TA_AT Cuts at: 160 1032 5338 160 Size: 872 4306 1606 Fragments arranged by size: 4306 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2240 2374 4109 1870 Size: 73 197 100 134 1735 4545 Fragments arranged by size: 4545 1735 197 134 100 73 BamHI G'GATC_C Cuts at: 2253 4114 4481 2253 Size: 1861 367 4556 Fragments arranged by size: 4556 1861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2467 3036 4105 5560 Size: 325 217 627 539 141 569 1069 1455 Cuts at: 5560 618 Size: 1842 Fragments arranged by size: 1842 1455 1069 627 569 539 325 217 141 BanII G_rGCy'C Cuts at: 1391 2207 2440 3206 4103 1391 Size: 816 233 766 897 4072 Fragments arranged by size: 4072 897 816 766 233 BbeI G_GCGC'C Cuts at: 2330 2471 2330 Size: 141 6643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 2865 3285 3312 3567 3843 928 Size: 971 856 110 420 27 255 276 3869 Fragments arranged by size: 3869 971 856 420 276 255 110 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4975 5009 1903 Size: 34 3038 34 3678 Fragments arranged by size: 3678 3038 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4676 5886 4676 Size: 1210 5574 BclI T'GATC_A Cuts at: 1548 2841 3270 1548 Size: 1293 429 5062 Fragments arranged by size: 5062 1293 429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5395 136 Size: 107 122 71 631 605 3723 1525 Fragments arranged by size: 3723 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3321 3357 6779 3321 Size: 36 3422 3326 Fragments arranged by size: 3422 3326 36 BlpI GC'TnA_GC Cuts at: 4201 4201 Size: 6784 Bme1580I G_kGCm'C Cuts at: 1790 2207 2440 4845 6001 1790 Size: 417 233 2405 1156 2573 Fragments arranged by size: 2573 2405 1156 417 233 BmgBI CAC'GTC Cuts at: 1318 2852 3871 1318 Size: 1534 1019 4231 Fragments arranged by size: 4231 1534 1019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2210 4515 5473 Size: 416 53 83 399 24 793 2305 958 Cuts at: 5473 442 Size: 1753 Fragments arranged by size: 2305 1753 958 793 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6784 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3901 3901 Size: 6784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2681 2702 2780 3190 5444 1239 Size: 893 549 21 78 410 2254 2579 Fragments arranged by size: 2579 2254 893 549 410 78 21 Bpu10I CC'TnA_GC Cuts at: 2048 2998 2048 Size: 950 5834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4909 5774 6072 2708 Size: 2201 865 298 3420 Fragments arranged by size: 3420 2201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2763 3368 3386 3470 5447 882 Size: 617 1264 605 18 84 1977 2219 Fragments arranged by size: 2219 1977 1264 617 605 84 18 BsaAI yAC'GTr Cuts at: 493 1204 4575 493 Size: 711 3371 2702 Fragments arranged by size: 3371 2702 711 BsaBI GATnn'nnATC Cuts at: 3878 6778 3878 Size: 2900 3884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2327 2468 3282 4591 Size: 53 83 186 936 794 141 814 1309 Cuts at: 4591 4973 5608 275 Size: 382 635 1451 Fragments arranged by size: 1451 1309 936 814 794 635 382 186 141 83 53 BsaWI w'CCGG_w Cuts at: 4079 5217 5615 5889 6036 6447 4079 Size: 1138 398 274 147 411 4416 Fragments arranged by size: 4416 1138 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6559 Fragments arranged by size: 6559 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2258 2569 2599 3980 1186 Size: 910 162 311 30 1381 3990 Fragments arranged by size: 3990 1381 910 311 162 30 BseYI C'CCAG_C Cuts at: 2130 2292 5987 6483 2130 Size: 162 3695 496 2431 Fragments arranged by size: 3695 2431 496 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3555 1163 Size: 573 1819 4392 Fragments arranged by size: 4392 1819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4234 4995 6023 1540 Size: 275 186 2233 761 1028 2301 Fragments arranged by size: 2301 2233 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4845 4930 6001 4845 Size: 85 1071 5628 Fragments arranged by size: 5628 1071 85 BsmI GAATG_Cn' Cuts at: 2810 4295 4388 2810 Size: 1485 93 5206 Fragments arranged by size: 5206 1485 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2726 2726 Size: 6784 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2440 3206 4103 4845 4930 6001 Size: 399 417 233 766 897 742 85 1071 Cuts at: 6001 1391 Size: 2174 Fragments arranged by size: 2174 1071 897 766 742 417 399 233 85 BspEI T'CCGG_A Cuts at: 6447 6447 Size: 6784 BspHI T'CATG_A Cuts at: 3346 4673 6403 3346 Size: 1327 1730 3727 Fragments arranged by size: 3727 1730 1327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2878 4139 844 Size: 2034 1261 3489 Fragments arranged by size: 3489 2034 1261 BsrBI CCG'CTC Cuts at: 1394 1823 2104 2542 2997 4671 1394 Size: 429 281 438 455 1674 3507 Fragments arranged by size: 3507 1674 455 438 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 3775 5279 5453 65 Size: 1183 2527 1504 174 1396 Fragments arranged by size: 2527 1504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3003 5428 5615 1700 Size: 110 1193 2425 187 2869 Fragments arranged by size: 2869 2425 1193 187 110 BsrGI T'GTAC_A Cuts at: 96 2407 96 Size: 2311 4473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6784 BssSI C'ACGA_G Cuts at: 4844 5856 4844 Size: 1012 5772 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6784 BstBI TT'CG_AA Cuts at: 5588 5588 Size: 6784 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6784 BstXI CCAn_nnnn'nTGG Cuts at: 2799 3033 4050 2799 Size: 234 1017 5533 Fragments arranged by size: 5533 1017 234 BstYI r'GATC_y Cuts at: 1972 2253 2677 2896 3321 3357 4114 4481 4866 Size: 281 424 219 425 36 757 367 385 Cuts at: 4866 4883 6335 6421 6433 6779 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 757 425 424 385 367 346 281 219 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2367 2367 Size: 6784 BtgI C'CryG_G Cuts at: 513 1065 2004 2337 2913 3658 513 Size: 552 939 333 576 745 3639 Fragments arranged by size: 3639 939 745 576 552 333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3807 487 Size: 683 56 2581 3464 Fragments arranged by size: 3464 2581 683 56 BtsI GCAGTG_nn' Cuts at: 2463 2694 3306 3760 4300 5094 5114 2463 Size: 231 612 454 540 794 20 4133 Fragments arranged by size: 4133 794 612 540 454 231 20 ClaI AT'CG_AT Cuts at: 1554 2394 1554 Size: 840 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6749 DraI TTT'AAA Cuts at: 4088 4088 Size: 6784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5791 809 Size: 507 4475 1802 Fragments arranged by size: 4475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2178 4231 Size: 54 1425 50 275 18 201 147 2053 Cuts at: 4231 5120 6749 8 Size: 889 1629 43 Fragments arranged by size: 2053 1629 1425 889 275 201 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4231 1537 Size: 275 2419 4090 Fragments arranged by size: 4090 2419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2486 2496 2600 3585 3900 4061 4714 1353 Size: 1133 10 104 985 315 161 653 3423 Fragments arranged by size: 3423 1133 985 653 315 161 104 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2596 5371 5745 5891 387 Size: 1064 138 1007 2775 374 146 1280 Fragments arranged by size: 2775 1280 1064 1007 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2681 2702 2780 3190 3579 4019 Size: 393 500 549 21 78 410 389 440 Cuts at: 4019 4847 5444 6231 6499 1239 Size: 828 597 787 268 1524 Fragments arranged by size: 1524 828 787 597 549 500 440 410 393 389 268 78 21 EcoNI CCTnn'n_nnAGG Cuts at: 3125 3125 Size: 6784 EcoO109I rG'GnC_Cy Cuts at: 2024 2204 2436 2437 3463 4039 2024 Size: 180 232 1 1026 576 4769 Fragments arranged by size: 4769 1026 576 232 180 1 EcoRI G'AATT_C Cuts at: 4093 4471 4093 Size: 378 6406 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 6784 FspI TGC'GCA Cuts at: 5290 5290 Size: 6784 HaeII r_GCGC'y Cuts at: 1678 2140 2330 2471 5931 1678 Size: 462 190 141 3460 2531 Fragments arranged by size: 3460 2531 462 190 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2760 2792 5425 5457 5499 Size: 32 135 32 1188 32 2633 32 42 Cuts at: 5499 5531 1373 Size: 32 2626 Fragments arranged by size: 2633 2626 1188 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 3172 4128 4374 669 Size: 988 281 547 687 956 246 3079 Fragments arranged by size: 3079 988 956 687 547 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6784 HpaI GTT'AAC Cuts at: 3172 4374 3172 Size: 1202 5582 KasI G'GCGC_C Cuts at: 2326 2467 2326 Size: 141 6643 KpnI G_GTAC'C Cuts at: 4109 4109 Size: 6784 MfeI C'AATT_G Cuts at: 4383 4383 Size: 6784 MluI A'CGCG_T Cuts at: 6660 6660 Size: 6784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2134 5898 6082 1305 Size: 829 3764 184 2007 Fragments arranged by size: 3764 2007 829 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4962 Fragments arranged by size: 4962 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2516 2741 2963 4742 5101 5260 518 Size: 648 1350 225 222 1779 359 159 2042 Fragments arranged by size: 2042 1779 1350 648 359 225 222 159 MspA1I CmG'CkG Cuts at: 1606 2119 2674 3033 3102 4200 4877 6025 6270 Size: 513 555 359 69 1098 677 1148 245 Cuts at: 6270 1606 Size: 2120 Fragments arranged by size: 2120 1148 1098 677 555 513 359 245 69 NaeI GCC'GGC Cuts at: 3005 3005 Size: 6784 NarI GG'CG_CC Cuts at: 2327 2468 2327 Size: 141 6643 NcoI C'CATG_G Cuts at: 513 1065 2004 2913 3658 513 Size: 552 939 909 745 3639 Fragments arranged by size: 3639 939 909 745 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6784 NgoMIV G'CCGG_C Cuts at: 3003 3003 Size: 6784 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6784 NotI GC'GGCC_GC Cuts at: 4231 4231 Size: 6784 NspI r_CATG'y Cuts at: 1132 1229 4142 5687 1132 Size: 97 2913 1545 2229 Fragments arranged by size: 2913 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6784 PciI A'CATG_T Cuts at: 5683 5683 Size: 6784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6784 PfoI T'CCnGG_A Cuts at: 2148 2757 3206 2148 Size: 609 449 5726 Fragments arranged by size: 5726 609 449 PmeI GTTT'AAAC Cuts at: 4088 4088 Size: 6784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4853 4885 6366 6398 4853 Size: 32 1481 32 5239 Fragments arranged by size: 5239 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3463 4039 3463 Size: 576 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6784 PsiI TTA'TAA Cuts at: 4354 4354 Size: 6784 PspOMI G'GGCC_C Cuts at: 2203 2436 2203 Size: 233 6551 PstI C_TGCA'G Cuts at: 830 2655 2889 4136 830 Size: 1825 234 1247 3478 Fragments arranged by size: 3478 1825 1247 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6784 PvuII CAG'CTG Cuts at: 2674 3102 2674 Size: 428 6356 SalI G'TCGA_C Cuts at: 1936 4126 1936 Size: 2190 4594 SapI GCTCTTCn'nnn_ Cuts at: 2486 2486 Size: 6784 SbfI CC_TGCA'GG Cuts at: 2655 4136 2655 Size: 1481 5303 ScaI AGT'ACT Cuts at: 3818 5032 6744 3818 Size: 1214 1712 3858 Fragments arranged by size: 3858 1712 1214 SfcI C'TryA_G Cuts at: 826 1045 2449 2651 2885 4132 5267 5948 6139 Size: 219 1404 202 234 1247 1135 681 191 Cuts at: 6139 826 Size: 1471 Fragments arranged by size: 1471 1404 1247 1135 681 234 219 202 191 SfoI GGC'GCC Cuts at: 2328 2469 2328 Size: 141 6643 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6784 SmaI CCC'GGG Cuts at: 1872 4111 1872 Size: 2239 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2723 4888 5789 6051 820 Size: 197 926 297 483 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 483 297 262 197 SnaBI TAC'GTA Cuts at: 493 4575 493 Size: 4082 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6784 SphI G_CATG'C Cuts at: 1132 4142 1132 Size: 3010 3774 SspI AAT'ATT Cuts at: 5 52 4708 5 Size: 47 4656 2081 Fragments arranged by size: 4656 2081 47 StuI AGG'CCT Cuts at: 1517 2372 1517 Size: 855 5929 StyI C'CwwG_G Cuts at: 513 1065 2004 2913 3658 4223 513 Size: 552 939 909 745 565 3074 Fragments arranged by size: 3074 939 909 745 565 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4811 4828 4981 5166 5611 325 Size: 1255 3231 17 153 185 445 1498 Fragments arranged by size: 3231 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2407 2934 3816 Size: 274 80 33 51 1443 430 527 882 Cuts at: 3816 5030 6742 96 Size: 1214 1712 138 Fragments arranged by size: 1712 1443 1214 882 527 430 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6744 6776 6744 Size: 32 6752 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6784 XbaI T'CTAG_A Cuts at: 4120 4120 Size: 6784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 2344 Size: 6784 XhoI C'TCGA_G Cuts at: 1943 2240 1943 Size: 297 6487 XmaI C'CCGG_G Cuts at: 1870 4109 1870 Size: 2239 4545 XmnI GAAnn'nnTTC Cuts at: 4913 4913 Size: 6784 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4592 276 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI