Restriction map for
pFN21AB8689
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI AlwNI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BciVI BclI BfrI BlnI BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 1187 ACGCGT A^C[GA][CT]GT
AgeI 1009 ACCGGT A^CCGGT
Alw44I 252 GTGCAC G^TGCAC
AocI 510 CCTCAGG CC^T[ACGT]AGG
Aor51HI 410 AGCGCT AGC^GCT
ApaLI 252 GTGCAC G^TGCAC
BalI 1082 TGGCCA TGG^CCA
BanII 712 GAGCTC G[GA]GC[CT]^C
BbrPI 805 CACGTG CAC^GTG
BbsI 1135 GTCTTC GAAGAC(2/6)
BbvCI 1000 GCTGAGG CCTCAGC(-5/-2)
BfaI 22 CTAG C^TAG
BglII 428 AGATCT A^GATCT
BlpI 620 GCTCAGC GC^T[ACGT]AGC
BpmI 171 CTGGAG CTGGAG(16/14)
Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC
BpuAI 1135 GTCTTC GAAGAC(2/6)
BsaAI 805 CACGTG [CT]AC^GT[GA]
BsaWI 1009 ACCGGT [AT]^CCGG[AT]
BseRI 1144 CTCCTC GAGGAG(10/8)
BspHI 326 TCATGA T^CATGA
BstXI 836 CCACCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 510 CCTCAGG CC^T[ACGT]AGG
CelII 620 GCTCAGC GC^T[ACGT]AGC
Eco47III 410 AGCGCT AGC^GCT
Eco81I 510 CCTCAGG CC^T[ACGT]AGG
HgaI 1133 GCGTC GACGC(5/10)
MaeI 22 CTAG C^TAG
MluI 1187 ACGCGT A^CGCGT
MluNI 1082 TGGCCA TGG^CCA
MscI 1082 TGGCCA TGG^CCA
MspA1I 623 CAGCGG C[AC]G^C[GT]G
NcoI 309 CCATGG C^CATGG
NspI 884 GCATGC [GA]CATG^[CT]
PflMI 801 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1009 ACCGGT A^CCGGT
PleI 1248 GACTC GAGTC(4/5)
PmaCI 805 CACGTG CAC^GTG
PmlI 805 CACGTG CAC^GTG
PstI 114 CTGCAG CTGCA^G
RcaI 326 TCATGA T^CATGA
SacI 712 GAGCTC GAGCT^C
SapI 444 GAAGAGC GCTCTTC(1/4)
SfaNI 1206 GATGC GCATC(5/9)
SfcI 114 CTGCAG C^T[GA][CT]AG
SgrAI 1008 CACCGGTG C[GA]^CCGG[CT]G
SmlI 151 CTCAAG C^T[CT][GA]AG
SphI 884 GCATGC GCATG^C
TfiI 86 GATTC G^A[AT]TC
Tsp509I 577 AATT ^AATT
Van91I 801 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 257 CGCG CG^CG
1188 CGCG
AcyI 553 GGCGCC G[GA]^CG[CT]C
1309 GGCGCC
AlwI 147 GATCC GGATC(4/5)
813 GATCC
AspHI 252 GTGCAC G[AT]GC[AT]^C
712 GAGCTC
AviII 236 TGCGCA TGC^GCA
377 TGCGCA
BbeI 553 GGCGCC GGCGC^C
1309 GGCGCC
BcgI 219 CGAGTTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
833 GCACCACCATCG
BglI 851 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
944 GCCTGCGGGGC
BsaHI 553 GGCGCC G[GA]^CG[CT]C
1309 GGCGCC
BsaI 1057 GAGACC GGTCTC(1/5)
931 GGTCTC
BsiHKAI 252 GTGCAC G[AT]GC[AT]^C
712 GAGCTC
BsrBI 562 CCGCTC CCGCTC(-3/-3)
1321 CCGCTC
BsrI 917 ACTGG ACTGG(1/-1)
1173 ACTGG
BstEII 283 GGTGACC G^GT[ACGT]ACC
594 GGTCACC
BstPI 283 GGTGACC G^GT[ACGT]ACC
594 GGTCACC
BstUI 257 CGCG CG^CG
1188 CGCG
EarI 493 CTCTTC CTCTTC(1/4)
444 GAAGAG
EcoO65I 283 GGTGACC G^GT[ACGT]ACC
594 GGTCACC
FspI 236 TGCGCA TGC^GCA
377 TGCGCA
Hin1I 553 GGCGCC G[GA]^CG[CT]C
1309 GGCGCC
HinfI 86 GATTC G^A[ACGT]TC
1248 GACTC
KasI 553 GGCGCC G^GCGCC
1309 GGCGCC
Ksp632I 493 CTCTTC CTCTTC(1/4)
444 GAAGAG
MvnI 257 CGCG CG^CG
1188 CGCG
NarI 553 GGCGCC GG^CGCC
1309 GGCGCC
SfoI 553 GGCGCC GGC^GCC
1309 GGCGCC
StuI 983 AGGCCT AGG^CCT
1127 AGGCCT
Tsp45I 284 GTGAC ^GT[GC]AC
595 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BbvI BcnI BmyI BsaJI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI StyI
TaqI TseI TspRI TthHB8I XhoII