(Circular) (Six-base) MAPSORT of: pFN21AB8689.seq Check: 6922 from: 1 to: 6055 pFN21AB8689 6055 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2288 2288 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2801 4118 5502 5770 1632 Size: 1169 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 1169 268 AfeI AGC'GCT Cuts at: 2417 2417 Size: 6055 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 3192 4954 5931 1203 Size: 1989 1762 977 1327 Fragments arranged by size: 1989 1762 1327 977 AgeI A'CCGG_T Cuts at: 3014 4886 3014 Size: 1872 4183 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2612 2846 1166 Size: 1446 234 4375 Fragments arranged by size: 4375 1446 234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 3798 5370 3798 Size: 1572 4483 ApaLI G'TGCA_C Cuts at: 2257 4112 5268 2257 Size: 1855 1156 3044 Fragments arranged by size: 3044 1855 1156 ApoI r'AATT_y Cuts at: 1493 3364 3590 3742 5851 1493 Size: 1871 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2222 2429 3059 3255 3380 1870 Size: 73 279 207 630 196 125 4545 Fragments arranged by size: 4545 630 279 207 196 125 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2558 2837 3011 3017 3314 Size: 325 217 627 771 279 174 6 297 Cuts at: 3314 3376 4831 618 Size: 62 1455 1842 Fragments arranged by size: 1842 1455 771 627 325 297 279 217 174 62 6 BanII G_rGCy'C Cuts at: 1391 2721 3374 1391 Size: 1330 653 4072 Fragments arranged by size: 4072 1330 653 BbeI G_GCGC'C Cuts at: 2562 3318 2562 Size: 756 5299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3133 928 Size: 971 1234 3850 Fragments arranged by size: 3850 1234 971 BbvCI CC'TCA_GC Cuts at: 3006 3006 Size: 6055 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2213 2247 2827 2861 4246 Size: 34 50 34 192 34 580 34 1385 Cuts at: 4246 4280 1903 Size: 34 3678 Fragments arranged by size: 3678 1385 580 192 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2862 2955 4666 Size: 107 122 71 631 605 1190 93 1711 Cuts at: 4666 136 Size: 1525 Fragments arranged by size: 1711 1525 1190 631 605 122 107 93 71 BglII A'GATC_T Cuts at: 2433 6050 2433 Size: 3617 2438 BlpI GC'TnA_GC Cuts at: 2626 3472 2626 Size: 846 5209 Bme1580I G_kGCm'C Cuts at: 1790 2261 3014 3022 4116 5272 1790 Size: 471 753 8 1094 1156 2573 Fragments arranged by size: 2573 1156 1094 753 471 8 BmgBI CAC'GTC Cuts at: 1318 2187 3166 1318 Size: 869 979 4207 Fragments arranged by size: 4207 979 869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2932 3786 4744 Size: 416 53 83 399 24 1515 854 958 Cuts at: 4744 442 Size: 1753 Fragments arranged by size: 1753 1515 958 854 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2197 4715 1239 Size: 958 2518 2579 Fragments arranged by size: 2579 2518 958 Bpu10I CC'TnA_GC Cuts at: 3006 3006 Size: 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 4180 5045 5343 2141 Size: 2039 865 298 2853 Fragments arranged by size: 2853 2039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2942 3056 4718 882 Size: 617 1443 114 1662 2219 Fragments arranged by size: 2219 1662 1443 617 114 BsaAI yAC'GTr Cuts at: 493 1204 2812 3846 493 Size: 711 1608 1034 2702 Fragments arranged by size: 2702 1608 1034 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2559 3315 3862 4244 Size: 53 83 186 936 1026 756 547 382 Cuts at: 4244 4879 275 Size: 635 1451 Fragments arranged by size: 1451 1026 936 756 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3014 4488 4886 5160 5307 5718 3014 Size: 1474 398 274 147 411 3351 Fragments arranged by size: 3351 1474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2333 2363 3107 3137 1178 Size: 30 165 30 930 30 744 30 4096 Fragments arranged by size: 4096 930 744 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3140 1186 Size: 1954 4101 BseYI C'CCAG_C Cuts at: 2203 5258 5754 2203 Size: 3055 496 2504 Fragments arranged by size: 3055 2504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2253 2573 2615 3030 3221 1163 Size: 573 517 320 42 415 191 3997 Fragments arranged by size: 3997 573 517 415 320 191 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2310 2400 2533 2727 3505 4266 Size: 275 186 309 90 133 194 778 761 Cuts at: 4266 5294 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 778 761 309 275 194 186 133 90 BsiHKAI G_wGCw'C Cuts at: 2261 2721 4116 4201 5272 2261 Size: 460 1395 85 1071 3044 Fragments arranged by size: 3044 1395 1071 460 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2721 3014 3022 3374 4116 4201 Size: 399 471 460 293 8 352 742 85 Cuts at: 4201 5272 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 471 460 399 352 293 85 8 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 2331 3944 5674 2331 Size: 1613 1730 2712 Fragments arranged by size: 2712 1730 1613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2288 2552 2587 3270 3410 844 Size: 1444 264 35 683 140 3489 Fragments arranged by size: 3489 1444 683 264 140 35 BsrBI CCG'CTC Cuts at: 1394 1823 2569 3328 3942 1394 Size: 429 746 759 614 3507 Fragments arranged by size: 3507 759 746 614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2395 3014 3109 4699 4886 1700 Size: 110 585 619 95 1590 187 2869 Fragments arranged by size: 2869 1590 619 585 187 110 95 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 2288 2599 1184 Size: 1104 311 4640 Fragments arranged by size: 4640 1104 311 BstXI CCAn_nnnn'nTGG Cuts at: 2848 2848 Size: 6055 BstYI r'GATC_y Cuts at: 1972 2151 2433 2817 3385 3752 4137 4154 5606 Size: 179 282 384 568 367 385 17 1452 Cuts at: 5606 5692 5704 6050 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 568 385 384 367 346 282 179 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2516 2516 Size: 6055 BtgI C'CryG_G Cuts at: 513 1065 2314 3084 3156 513 Size: 552 1249 770 72 3412 Fragments arranged by size: 3412 1249 770 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2634 3571 4365 4385 2634 Size: 937 794 20 4304 Fragments arranged by size: 4304 937 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2307 2397 Size: 54 1425 50 275 18 266 211 90 Cuts at: 2397 2469 2564 2631 2724 3087 3180 3502 4391 Size: 72 95 67 93 363 93 322 889 Cuts at: 4391 6020 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 363 322 275 266 211 95 93 93 90 72 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2307 2397 2724 3502 1537 Size: 275 495 90 327 778 4090 Fragments arranged by size: 4090 778 495 327 275 90 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 2504 3985 1353 Size: 1091 60 1481 3423 Fragments arranged by size: 3423 1481 1091 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2281 4642 5016 5162 387 Size: 1064 138 692 2361 374 146 1280 Fragments arranged by size: 2361 1280 1064 692 374 146 138 EcoICRI GAG'CTC Cuts at: 2719 2719 Size: 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2801 4118 4715 5502 5770 1239 Size: 393 565 604 1317 597 787 268 1524 Fragments arranged by size: 1524 1317 787 604 597 565 393 268 EcoO109I rG'GnC_Cy Cuts at: 2326 2506 3118 3259 2326 Size: 180 612 141 5122 Fragments arranged by size: 5122 612 180 141 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 2243 2384 4561 2243 Size: 141 2177 3737 Fragments arranged by size: 3737 2177 141 HaeII r_GCGC'y Cuts at: 1678 2184 2419 2562 3318 5202 1678 Size: 506 235 143 756 1884 2531 Fragments arranged by size: 2531 1884 756 506 235 143 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 4696 4728 4770 Size: 32 135 32 1211 32 1881 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1881 1211 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2558 3314 2558 Size: 756 5299 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 3192 5931 3192 Size: 2739 3316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3177 3246 5169 5353 1305 Size: 1872 69 1923 184 2007 Fragments arranged by size: 2007 1923 1872 184 69 MscI TGG'CCA Cuts at: 10 64 1489 1832 3089 10 Size: 54 1425 343 1257 2976 Fragments arranged by size: 2976 1425 1257 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 2846 2849 3000 3243 4013 4372 Size: 648 1446 234 3 151 243 770 359 Cuts at: 4372 4531 518 Size: 159 2042 Fragments arranged by size: 2042 1446 770 648 359 243 234 159 151 3 MspA1I CmG'CkG Cuts at: 1606 2630 3471 4148 5296 5541 1606 Size: 1024 841 677 1148 245 2120 Fragments arranged by size: 2120 1148 1024 841 677 245 NarI GG'CG_CC Cuts at: 2559 3315 2559 Size: 756 5299 NcoI C'CATG_G Cuts at: 513 1065 2314 513 Size: 552 1249 4254 Fragments arranged by size: 4254 1249 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2893 3413 4958 1132 Size: 97 1664 520 1545 2229 Fragments arranged by size: 2229 1664 1545 520 97 PasI CC'CwG_GG Cuts at: 1348 2086 1348 Size: 738 5317 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2812 1108 Size: 1704 4351 PfoI T'CCnGG_A Cuts at: 2673 2673 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PmlI CAC'GTG Cuts at: 2812 2812 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2506 3118 3259 2326 Size: 180 612 141 5122 Fragments arranged by size: 5122 612 180 141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PstI C_TGCA'G Cuts at: 830 2123 3407 830 Size: 1293 1284 3478 Fragments arranged by size: 3478 1293 1284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 SacI G_AGCT'C Cuts at: 2721 2721 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SanDI GG'GwC_CC Cuts at: 2506 2506 Size: 6055 SapI GCTCTTCn'nnn_ Cuts at: 2444 2444 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2119 3403 4538 5219 5410 826 Size: 219 1074 1284 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 1074 681 219 191 SfoI GGC'GCC Cuts at: 2560 3316 2560 Size: 756 5299 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 3014 1700 Size: 1314 4741 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 4159 5060 5322 820 Size: 197 926 213 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 262 213 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 2893 3413 1132 Size: 1761 520 3774 Fragments arranged by size: 3774 1761 520 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2990 3134 1517 Size: 1473 144 4438 Fragments arranged by size: 4438 1473 144 StyI C'CwwG_G Cuts at: 513 1065 2015 2314 2352 3494 513 Size: 552 950 299 38 1142 3074 Fragments arranged by size: 3074 1142 950 552 299 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3064 4082 4099 4252 4437 4882 325 Size: 1255 1484 1018 17 153 185 445 1498 Fragments arranged by size: 1498 1484 1255 1018 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 3123 4301 Size: 274 80 33 51 1443 852 294 1178 Cuts at: 4301 6013 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1178 852 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2333 2365 6015 6047 2333 Size: 32 3650 32 2341 Fragments arranged by size: 3650 2341 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI XcmI