Restriction map for
pFN21AB8689
(Linear) (Six-base) MAPSORT of: KIBB8689.seq Check: 5910 from: 1 to: 1350
KIBB8689.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 283 1350
Size: 283 1067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1350
Size: 796 554
AfeI AGC'GCT
Cuts at: 0 412 1350
Size: 412 938
AflIII A'CryG_T
Cuts at: 0 1187 1350
Size: 1187 163
AgeI A'CCGG_T
Cuts at: 0 1009 1350
Size: 1009 341
AleI CACnn'nnGTG
Cuts at: 0 607 841 1350
Size: 607 234 509
Fragments arranged by size:
607 509 234
ApaLI G'TGCA_C
Cuts at: 0 252 1350
Size: 252 1098
AvaI C'yCGr_G
Cuts at: 0 217 424 1054 1250 1350
Size: 217 207 630 196 100
Fragments arranged by size:
630 217 207 196 100
BanI G'GyrC_C
Cuts at: 0 553 832 1006 1012 1309 1350
Size: 553 279 174 6 297 41
Fragments arranged by size:
553 297 279 174 41 6
BanII G_rGCy'C
Cuts at: 0 716 1350
Size: 716 634
BbeI G_GCGC'C
Cuts at: 0 557 1313 1350
Size: 557 756 37
Fragments arranged by size:
756 557 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 1128 1350
Size: 1128 222
BbvCI CC'TCA_GC
Cuts at: 0 1001 1350
Size: 1001 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 822 856 1350
Size: 208 34 580 34 494
Fragments arranged by size:
580 494 208 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 857 950 1350
Size: 857 93 400
Fragments arranged by size:
857 400 93
BglII A'GATC_T
Cuts at: 0 428 1350
Size: 428 922
BlpI GC'TnA_GC
Cuts at: 0 621 1350
Size: 621 729
Bme1580I G_kGCm'C
Cuts at: 0 256 1009 1017 1350
Size: 256 753 8 333
Fragments arranged by size:
753 333 256 8
BmgBI CAC'GTC
Cuts at: 0 182 1161 1350
Size: 182 979 189
Fragments arranged by size:
979 189 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 927 1350
Size: 927 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 1350
Size: 192 1158
Bpu10I CC'TnA_GC
Cuts at: 0 1001 1350
Size: 1001 349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1350
Size: 136 1214
BsaI GGTCTCn'nnnn_
Cuts at: 0 937 1051 1350
Size: 937 114 299
Fragments arranged by size:
937 299 114
BsaAI yAC'GTr
Cuts at: 0 807 1350
Size: 807 543
BsaHI Gr'CG_yC
Cuts at: 0 554 1310 1350
Size: 554 756 40
Fragments arranged by size:
756 554 40
BsaWI w'CCGG_w
Cuts at: 0 1009 1350
Size: 1009 341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 328 358 1102 1132 1350
Size: 328 30 744 30 218
Fragments arranged by size:
744 328 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1135 1350
Size: 1135 215
BseYI C'CCAG_C
Cuts at: 0 198 1350
Size: 198 1152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 568 610 1025 1216 1350
Size: 248 320 42 415 191 134
Fragments arranged by size:
415 320 248 191 134 42
BsiEI CG_ry'CG
Cuts at: 0 305 395 528 722 1350
Size: 305 90 133 194 628
Fragments arranged by size:
628 305 194 133 90
BsiHKAI G_wGCw'C
Cuts at: 0 256 716 1350
Size: 256 460 634
Fragments arranged by size:
634 460 256
Bsp1286I G_dGCh'C
Cuts at: 0 256 716 1009 1017 1350
Size: 256 460 293 8 333
Fragments arranged by size:
460 333 293 256 8
BspHI T'CATG_A
Cuts at: 0 326 1350
Size: 326 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 283 547 582 1265 1350
Size: 283 264 35 683 85
Fragments arranged by size:
683 283 264 85 35
BsrBI CCG'CTC
Cuts at: 0 564 1323 1350
Size: 564 759 27
Fragments arranged by size:
759 564 27
BsrFI r'CCGG_y
Cuts at: 0 390 1009 1104 1350
Size: 390 619 95 246
Fragments arranged by size:
619 390 246 95
BstEII G'GTnAC_C
Cuts at: 0 283 594 1350
Size: 283 311 756
Fragments arranged by size:
756 311 283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1350
Size: 843 507
BstYI r'GATC_y
Cuts at: 0 146 428 812 1350
Size: 146 282 384 538
Fragments arranged by size:
538 384 282 146
Bsu36I CC'TnA_GG
Cuts at: 0 511 1350
Size: 511 839
BtgI C'CryG_G
Cuts at: 0 309 1079 1151 1350
Size: 309 770 72 199
Fragments arranged by size:
770 309 199 72
BtsI GCAGTG_nn'
Cuts at: 0 629 1350
Size: 629 721
EaeI y'GGCC_r
Cuts at: 0 91 302 392 464 559 626 719 1082
Size: 91 211 90 72 95 67 93 363
Cuts at: 1082 1175 1350
Size: 93 175
Fragments arranged by size:
363 211 175 95 93 93 91 90
72 67
EagI C'GGCC_G
Cuts at: 0 302 392 719 1350
Size: 302 90 327 631
Fragments arranged by size:
631 327 302 90
EarI CTCTTCn'nnn_
Cuts at: 0 439 499 1350
Size: 439 60 851
Fragments arranged by size:
851 439 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 276 1350
Size: 276 1074
EcoICRI GAG'CTC
Cuts at: 0 714 1350
Size: 714 636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 796 1350
Size: 192 604 554
Fragments arranged by size:
604 554 192
EcoO109I rG'GnC_Cy
Cuts at: 0 321 501 1113 1254 1350
Size: 321 180 612 141 96
Fragments arranged by size:
612 321 180 141 96
FspI TGC'GCA
Cuts at: 0 238 379 1350
Size: 238 141 971
Fragments arranged by size:
971 238 141
HaeII r_GCGC'y
Cuts at: 0 179 414 557 1313 1350
Size: 179 235 143 756 37
Fragments arranged by size:
756 235 179 143 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 1350
Size: 778 32 540
Fragments arranged by size:
778 540 32
KasI G'GCGC_C
Cuts at: 0 553 1309 1350
Size: 553 756 41
Fragments arranged by size:
756 553 41
MluI A'CGCG_T
Cuts at: 0 1187 1350
Size: 1187 163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 1241 1350
Size: 1172 69 109
Fragments arranged by size:
1172 109 69
MscI TGG'CCA
Cuts at: 0 1084 1350
Size: 1084 266
MslI CAynn'nnrTG
Cuts at: 0 607 841 844 995 1238 1350
Size: 607 234 3 151 243 112
Fragments arranged by size:
607 243 234 151 112 3
MspA1I CmG'CkG
Cuts at: 0 625 1350
Size: 625 725
NarI GG'CG_CC
Cuts at: 0 554 1310 1350
Size: 554 756 40
Fragments arranged by size:
756 554 40
NcoI C'CATG_G
Cuts at: 0 309 1350
Size: 309 1041
NspI r_CATG'y
Cuts at: 0 888 1350
Size: 888 462
PasI CC'CwG_GG
Cuts at: 0 81 1350
Size: 81 1269
PflMI CCAn_nnn'nTGG
Cuts at: 0 807 1350
Size: 807 543
PfoI T'CCnGG_A
Cuts at: 0 668 1350
Size: 668 682
PmlI CAC'GTG
Cuts at: 0 807 1350
Size: 807 543
PpuMI rG'GwC_Cy
Cuts at: 0 321 501 1113 1254 1350
Size: 321 180 612 141 96
Fragments arranged by size:
612 321 180 141 96
PstI C_TGCA'G
Cuts at: 0 118 1350
Size: 118 1232
SacI G_AGCT'C
Cuts at: 0 716 1350
Size: 716 634
SanDI GG'GwC_CC
Cuts at: 0 501 1350
Size: 501 849
SapI GCTCTTCn'nnn_
Cuts at: 0 439 1350
Size: 439 911
SfcI C'TryA_G
Cuts at: 0 114 1350
Size: 114 1236
SfoI GGC'GCC
Cuts at: 0 555 1311 1350
Size: 555 756 39
Fragments arranged by size:
756 555 39
SgrAI Cr'CCGG_yG
Cuts at: 0 1009 1350
Size: 1009 341
SmlI C'TyrA_G
Cuts at: 0 151 1350
Size: 151 1199
SphI G_CATG'C
Cuts at: 0 888 1350
Size: 888 462
StuI AGG'CCT
Cuts at: 0 985 1129 1350
Size: 985 144 221
Fragments arranged by size:
985 221 144
StyI C'CwwG_G
Cuts at: 0 10 309 347 1350
Size: 10 299 38 1003
Fragments arranged by size:
1003 299 38 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1059 1350
Size: 1059 291
TatI w'GTAC_w
Cuts at: 0 824 1118 1350
Size: 824 294 232
Fragments arranged by size:
824 294 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 328 360 1350
Size: 328 32 990
Fragments arranged by size:
990 328 32
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AleI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI MluI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PmlI PpuMI PstI SacI SanDI SapI
SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AlfI AloI
AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BmtI BplI BsaBI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI