(Circular) (Six-base) MAPSORT of: pFN21AB8688.seq Check: 4078 from: 1 to: 6211 pFN21AB8688 6211 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2110 3554 1937 Size: 173 1444 4594 Fragments arranged by size: 4594 1444 173 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 3371 4274 5658 5926 1632 Size: 591 1148 903 1384 268 1917 Fragments arranged by size: 1917 1384 1148 903 591 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3014 1184 Size: 1830 4381 AlwNI CAG_nnn'CTG Cuts at: 3166 3954 5526 3166 Size: 788 1572 3851 Fragments arranged by size: 3851 1572 788 ApaLI G'TGCA_C Cuts at: 3049 4268 5424 3049 Size: 1219 1156 3836 Fragments arranged by size: 3836 1219 1156 ApoI r'AATT_y Cuts at: 1493 2084 2275 2902 3401 3520 3746 3898 6007 Size: 591 191 627 499 119 226 152 2109 Cuts at: 6007 1493 Size: 1697 Fragments arranged by size: 2109 1697 627 591 499 226 191 152 119 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 2403 3495 3536 1870 Size: 73 460 1092 41 4545 Fragments arranged by size: 4545 1092 460 73 41 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3532 4987 618 Size: 325 217 627 1745 1455 1842 Fragments arranged by size: 1842 1745 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2943 3530 1391 Size: 1552 587 4072 Fragments arranged by size: 4072 1552 587 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 2742 2846 3489 928 Size: 971 600 243 104 643 3650 Fragments arranged by size: 3650 971 643 600 243 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4402 4436 1903 Size: 34 2465 34 3678 Fragments arranged by size: 3678 2465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 2852 4822 Size: 107 122 71 631 605 623 557 1970 Cuts at: 4822 136 Size: 1525 Fragments arranged by size: 1970 1525 631 623 605 557 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 3628 3628 Size: 6211 Bme1580I G_kGCm'C Cuts at: 1790 3053 3123 4272 5428 1790 Size: 1263 70 1149 1156 2573 Fragments arranged by size: 2573 1263 1156 1149 70 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3942 4900 442 Size: 416 53 83 399 24 2525 958 1753 Fragments arranged by size: 2525 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 4871 1239 Size: 1292 2340 2579 Fragments arranged by size: 2579 2340 1292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4336 5201 5499 4336 Size: 865 298 5048 Fragments arranged by size: 5048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 2466 6205 2466 Size: 3739 2472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4018 4400 5035 275 Size: 53 83 186 936 2485 382 635 1451 Fragments arranged by size: 2485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3495 3525 1178 Size: 30 165 30 2092 30 3864 Fragments arranged by size: 3864 2092 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 1186 Size: 1468 4743 BseYI C'CCAG_C Cuts at: 3131 3173 5414 5910 3131 Size: 42 2241 496 3432 Fragments arranged by size: 3432 2241 496 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 1163 Size: 573 310 5328 Fragments arranged by size: 5328 573 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 2943 3053 4272 4357 5428 2861 Size: 82 110 1219 85 1071 3644 Fragments arranged by size: 3644 1219 1071 110 85 82 BsiWI C'GTAC_G Cuts at: 3219 3219 Size: 6211 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 BsmBI CGTCTCn'nnnn_ Cuts at: 3188 3188 Size: 6211 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2861 2943 3053 3123 3530 4272 4357 Size: 399 1071 82 110 70 407 742 85 Cuts at: 4357 5428 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1071 742 407 399 110 85 82 70 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 4100 5830 4100 Size: 1730 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 2785 3566 844 Size: 1290 651 781 3489 Fragments arranged by size: 3489 1290 781 651 BsrBI CCG'CTC Cuts at: 1394 1823 2233 3181 4098 1394 Size: 429 410 948 917 3507 Fragments arranged by size: 3507 948 917 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 2893 4706 4880 65 Size: 1183 1645 1813 174 1396 Fragments arranged by size: 1813 1645 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 2877 794 Size: 2083 4128 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 3088 1184 Size: 1904 4307 BstYI r'GATC_y Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206 Size: 1569 367 385 17 1452 86 12 346 Cuts at: 6206 1972 Size: 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3084 513 Size: 552 939 1080 3640 Fragments arranged by size: 3640 1080 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 2935 3191 3727 4521 4541 2935 Size: 256 536 794 20 4605 Fragments arranged by size: 4605 794 536 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3658 4547 6176 Size: 54 1425 50 275 18 1828 889 1629 Cuts at: 6176 8 Size: 43 Fragments arranged by size: 1828 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2532 2691 3330 4141 1353 Size: 846 333 159 639 811 3423 Fragments arranged by size: 3423 846 811 639 333 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2474 4798 5172 5318 387 Size: 1064 138 885 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 885 374 146 138 EcoICRI GAG'CTC Cuts at: 2941 2941 Size: 6211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2531 3371 4274 4871 5658 5926 Size: 393 591 308 840 903 597 787 268 Cuts at: 5926 1239 Size: 1524 Fragments arranged by size: 1524 903 840 787 597 591 393 308 268 EcoO109I rG'GnC_Cy Cuts at: 2644 2644 Size: 6211 EcoRI G'AATT_C Cuts at: 2275 2902 3520 3898 2275 Size: 627 618 378 4588 Fragments arranged by size: 4588 627 618 378 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 5358 1678 Size: 3680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 4852 4884 4926 Size: 32 135 32 1475 32 1773 32 42 Cuts at: 4926 4958 1373 Size: 32 2626 Fragments arranged by size: 2626 1773 1475 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3205 3555 3801 669 Size: 988 281 1267 350 246 3079 Fragments arranged by size: 3079 1267 988 350 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5325 5509 1305 Size: 4020 184 2007 Fragments arranged by size: 4020 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4389 Fragments arranged by size: 4389 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2996 3459 4169 4528 4687 518 Size: 648 1830 463 710 359 159 2042 Fragments arranged by size: 2042 1830 710 648 463 359 159 MspA1I CmG'CkG Cuts at: 1606 3135 3627 4304 5452 5697 1606 Size: 1529 492 677 1148 245 2120 Fragments arranged by size: 2120 1529 1148 677 492 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4720 Fragments arranged by size: 4720 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 3130 3569 5114 1132 Size: 97 1901 439 1545 2229 Fragments arranged by size: 2229 1901 1545 439 97 PasI CC'CwG_GG Cuts at: 1348 2607 1348 Size: 1259 4952 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2196 3077 1108 Size: 1088 881 4242 Fragments arranged by size: 4242 1088 881 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2644 2644 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 3200 1613 Size: 1587 4624 PsiI TTA'TAA Cuts at: 2708 3781 2708 Size: 1073 5138 PstI C_TGCA'G Cuts at: 830 2782 3162 3563 830 Size: 1952 380 401 3478 Fragments arranged by size: 3478 1952 401 380 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 3135 3135 Size: 6211 SacI G_AGCT'C Cuts at: 2943 2943 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2532 Size: 6211 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2778 3158 3559 4694 5375 5566 826 Size: 219 1733 380 401 1135 681 191 1471 Fragments arranged by size: 1733 1471 1135 681 401 380 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4315 5216 5478 820 Size: 197 926 2372 901 262 1553 Fragments arranged by size: 2372 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 2551 3262 4135 5 Size: 47 2499 711 873 2081 Fragments arranged by size: 2499 2081 873 711 47 StuI AGG'CCT Cuts at: 1517 3146 1517 Size: 1629 4582 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 3650 513 Size: 552 939 651 995 3074 Fragments arranged by size: 3074 995 939 651 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2012 2936 4238 4255 4408 4593 5038 Size: 1255 432 924 1302 17 153 185 445 Cuts at: 5038 325 Size: 1498 Fragments arranged by size: 1498 1302 1255 924 445 432 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2158 2330 4457 Size: 274 80 33 51 1443 181 172 2127 Cuts at: 4457 6169 96 Size: 1712 138 Fragments arranged by size: 2127 1712 1443 274 181 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 6171 6203 2228 Size: 32 3911 32 2236 Fragments arranged by size: 3911 2236 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 3314 4340 3314 Size: 1026 5185 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI