Restriction map for
pFN21AB8688
(Linear) (Six-base) MAPSORT of: KIBB8688.seq Check: 1600 from: 1 to: 1506
KIBB8688.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 105 1506
Size: 105 1401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1366 1506
Size: 218 1148 140
Fragments arranged by size:
1148 218 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1009 1506
Size: 1009 497
AlwNI CAG_nnn'CTG
Cuts at: 0 1161 1506
Size: 1161 345
ApaLI G'TGCA_C
Cuts at: 0 1044 1506
Size: 1044 462
ApoI r'AATT_y
Cuts at: 0 79 270 897 1396 1506
Size: 79 191 627 499 110
Fragments arranged by size:
627 499 191 110 79
AvaI C'yCGr_G
Cuts at: 0 398 1490 1506
Size: 398 1092 16
Fragments arranged by size:
1092 398 16
BanII G_rGCy'C
Cuts at: 0 938 1506
Size: 938 568
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 737 841 1484 1506
Size: 494 243 104 643 22
Fragments arranged by size:
643 494 243 104 22
BglI GCCn_nnn'nGGC
Cuts at: 0 290 847 1506
Size: 290 557 659
Fragments arranged by size:
659 557 290
Bme1580I G_kGCm'C
Cuts at: 0 1048 1118 1506
Size: 1048 70 388
Fragments arranged by size:
1048 388 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1506
Size: 526 980
BsaBI GATnn'nnATC
Cuts at: 0 461 1506
Size: 461 1045
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 1506
Size: 649 857
BseYI C'CCAG_C
Cuts at: 0 1126 1168 1506
Size: 1126 42 338
Fragments arranged by size:
1126 338 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1506
Size: 41 1465
BsiHKAI G_wGCw'C
Cuts at: 0 856 938 1048 1506
Size: 856 82 110 458
Fragments arranged by size:
856 458 110 82
BsiWI C'GTAC_G
Cuts at: 0 1214 1506
Size: 1214 292
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1183 1506
Size: 1183 323
Bsp1286I G_dGCh'C
Cuts at: 0 856 938 1048 1118 1506
Size: 856 82 110 70 388
Fragments arranged by size:
856 388 110 82 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 780 1506
Size: 129 651 726
Fragments arranged by size:
726 651 129
BsrBI CCG'CTC
Cuts at: 0 228 1176 1506
Size: 228 948 330
Fragments arranged by size:
948 330 228
BsrDI GCAATG_nn'
Cuts at: 0 888 1506
Size: 888 618
BstAPI GCAn_nnn'nTGC
Cuts at: 0 872 1506
Size: 872 634
BstEII G'GTnAC_C
Cuts at: 0 1083 1506
Size: 1083 423
BtgI C'CryG_G
Cuts at: 0 1079 1506
Size: 1079 427
BtsI GCAGTG_nn'
Cuts at: 0 930 1186 1506
Size: 930 256 320
Fragments arranged by size:
930 320 256
EarI CTCTTCn'nnn_
Cuts at: 0 194 527 686 1325 1506
Size: 194 333 159 639 181
Fragments arranged by size:
639 333 194 181 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 1506
Size: 469 1037
EcoICRI GAG'CTC
Cuts at: 0 936 1506
Size: 936 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 526 1366 1506
Size: 218 308 840 140
Fragments arranged by size:
840 308 218 140
EcoO109I rG'GnC_Cy
Cuts at: 0 639 1506
Size: 639 867
EcoRI G'AATT_C
Cuts at: 0 270 897 1506
Size: 270 627 609
Fragments arranged by size:
627 609 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1506
Size: 1042 32 432
Fragments arranged by size:
1042 432 32
HincII GTy'rAC
Cuts at: 0 1200 1506
Size: 1200 306
MslI CAynn'nnrTG
Cuts at: 0 991 1454 1506
Size: 991 463 52
Fragments arranged by size:
991 463 52
MspA1I CmG'CkG
Cuts at: 0 1130 1506
Size: 1130 376
NspI r_CATG'y
Cuts at: 0 1125 1506
Size: 1125 381
PasI CC'CwG_GG
Cuts at: 0 602 1506
Size: 602 904
PflMI CCAn_nnn'nTGG
Cuts at: 0 191 1072 1506
Size: 191 881 434
Fragments arranged by size:
881 434 191
PpuMI rG'GwC_Cy
Cuts at: 0 639 1506
Size: 639 867
PshAI GACnn'nnGTC
Cuts at: 0 1195 1506
Size: 1195 311
PsiI TTA'TAA
Cuts at: 0 703 1506
Size: 703 803
PstI C_TGCA'G
Cuts at: 0 777 1157 1506
Size: 777 380 349
Fragments arranged by size:
777 380 349
PvuII CAG'CTG
Cuts at: 0 1130 1506
Size: 1130 376
SacI G_AGCT'C
Cuts at: 0 938 1506
Size: 938 568
SapI GCTCTTCn'nnn_
Cuts at: 0 527 1506
Size: 527 979
SfcI C'TryA_G
Cuts at: 0 773 1153 1506
Size: 773 380 353
Fragments arranged by size:
773 380 353
SspI AAT'ATT
Cuts at: 0 546 1257 1506
Size: 546 711 249
Fragments arranged by size:
711 546 249
StuI AGG'CCT
Cuts at: 0 1141 1506
Size: 1141 365
StyI C'CwwG_G
Cuts at: 0 650 1506
Size: 650 856
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 931 1506
Size: 7 924 575
Fragments arranged by size:
924 575 7
TatI w'GTAC_w
Cuts at: 0 153 325 1506
Size: 153 172 1181
Fragments arranged by size:
1181 172 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 1506
Size: 223 32 1251
Fragments arranged by size:
1251 223 32
XmnI GAAnn'nnTTC
Cuts at: 0 1309 1506
Size: 1309 197
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BanII
BbsI BglI Bme1580I BpmI BsaBI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstEII BtgI BtsI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HincII MslI MspA1I NspI PasI PflMI
PpuMI PshAI PsiI PstI PvuII SacI SapI SfcI
SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsiEI BsmI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI