(Circular) (Six-base) MAPSORT of: pFN21AB8686.seq Check: 1072 from: 1 to: 5917 pFN21AB8686 5917 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3120 3980 5364 5632 1632 Size: 1488 860 1384 268 1917 Fragments arranged by size: 1917 1488 1384 860 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2808 4816 5793 1203 Size: 1605 2008 977 1327 Fragments arranged by size: 2008 1605 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2047 3660 5232 2047 Size: 1613 1572 2732 Fragments arranged by size: 2732 1613 1572 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 2827 3070 3226 3452 3604 5713 1493 Size: 1334 243 156 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 243 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 2418 4471 160 Size: 872 1386 2053 1606 Fragments arranged by size: 2053 1606 1386 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2086 3242 1870 Size: 73 143 1156 4545 Fragments arranged by size: 4545 1156 143 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3238 4693 618 Size: 325 217 627 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 627 325 217 BanII G_rGCy'C Cuts at: 1391 2138 3236 1391 Size: 747 1098 4072 Fragments arranged by size: 4072 1098 747 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2756 3195 928 Size: 971 857 439 3650 Fragments arranged by size: 3650 971 857 439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2849 3809 5019 2849 Size: 960 1210 3747 Fragments arranged by size: 3747 1210 960 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 3978 5134 1790 Size: 2188 1156 2573 Fragments arranged by size: 2573 2188 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2688 4577 1239 Size: 830 619 1889 2579 Fragments arranged by size: 2579 1889 830 619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 2154 5911 2154 Size: 3757 2160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2104 1186 Size: 900 18 4999 Fragments arranged by size: 4999 900 18 BseYI C'CCAG_C Cuts at: 2789 5120 5616 2789 Size: 2331 496 3090 Fragments arranged by size: 3090 2331 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2775 1163 Size: 573 1039 4305 Fragments arranged by size: 4305 1039 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 3367 4128 5156 1540 Size: 275 186 112 1254 761 1028 2301 Fragments arranged by size: 2301 1254 1028 761 275 186 112 BsiHKAI G_wGCw'C Cuts at: 2138 2695 3978 4063 5134 2138 Size: 557 1283 85 1071 2921 Fragments arranged by size: 2921 1283 1071 557 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2695 3236 3978 4063 5134 1391 Size: 399 348 557 541 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 541 399 348 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 3272 844 Size: 1227 1201 3489 Fragments arranged by size: 3489 1227 1201 BsrBI CCG'CTC Cuts at: 1394 1823 2053 2094 3804 1394 Size: 429 230 41 1710 3507 Fragments arranged by size: 3507 1710 429 230 41 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4412 4586 65 Size: 1183 1623 1541 174 1396 Fragments arranged by size: 1623 1541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 2153 4721 2153 Size: 2568 3349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2793 3247 3614 3999 4016 5468 5554 5566 Size: 821 454 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 821 454 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4426 Fragments arranged by size: 4426 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2966 3433 4227 4247 2966 Size: 467 794 20 4636 Fragments arranged by size: 4636 794 467 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2110 3198 3364 Size: 54 1425 50 275 18 280 1088 166 Cuts at: 3364 4253 5882 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1088 889 280 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2110 3364 1537 Size: 275 298 1254 4090 Fragments arranged by size: 4090 1254 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2988 2994 3051 3847 1353 Size: 1635 6 57 796 3423 Fragments arranged by size: 3423 1635 796 57 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4504 4878 5024 387 Size: 1064 138 435 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2136 2136 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2688 3120 3980 4577 5364 5632 Size: 393 437 619 432 860 597 787 268 Cuts at: 5632 1239 Size: 1524 Fragments arranged by size: 1524 860 787 619 597 437 432 393 268 EcoO109I rG'GnC_Cy Cuts at: 2083 2083 Size: 5917 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2026 5064 1678 Size: 348 3038 2531 Fragments arranged by size: 3038 2531 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 4558 4590 4632 Size: 32 135 32 1454 32 1500 32 42 Cuts at: 4632 4664 1373 Size: 32 2626 Fragments arranged by size: 2626 1500 1454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3261 3507 669 Size: 988 281 1221 102 246 3079 Fragments arranged by size: 3079 1221 988 281 246 102 HindIII A'AGCT_T Cuts at: 748 3181 748 Size: 2433 3484 HpaI GTT'AAC Cuts at: 3159 3507 3159 Size: 348 5569 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2383 5031 5215 1305 Size: 1078 2648 184 2007 Fragments arranged by size: 2648 2007 1078 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3200 10 Size: 54 1425 343 1368 2727 Fragments arranged by size: 2727 1425 1368 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3875 4234 4393 518 Size: 648 2709 359 159 2042 Fragments arranged by size: 2709 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 3333 4010 5158 5403 1606 Size: 429 1298 677 1148 245 2120 Fragments arranged by size: 2120 1298 1148 677 429 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4426 Fragments arranged by size: 4426 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 4978 PfoI T'CCnGG_A Cuts at: 2040 2665 2040 Size: 625 5292 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 5917 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 2660 3487 2660 Size: 827 5090 PstI C_TGCA'G Cuts at: 830 2031 2082 3269 830 Size: 1201 51 1187 3478 Fragments arranged by size: 3478 1201 1187 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 SacI G_AGCT'C Cuts at: 2138 2138 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SfcI C'TryA_G Cuts at: 826 1045 2027 2078 2161 3265 4400 5081 5272 Size: 219 982 51 83 1104 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1471 1135 1104 982 681 219 191 83 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4021 4922 5184 820 Size: 197 926 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 2719 3841 5 Size: 47 2667 1122 2081 Fragments arranged by size: 2667 2081 1122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2004 2851 3356 513 Size: 552 939 847 505 3074 Fragments arranged by size: 3074 939 847 552 505 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 3944 3961 4114 4299 4744 325 Size: 1255 1188 1176 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1188 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2440 2800 4163 Size: 274 80 33 51 1443 463 360 1363 Cuts at: 4163 5875 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1363 463 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3128 5877 5909 3096 Size: 32 2749 32 3104 Fragments arranged by size: 3104 2749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2779 2779 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 2472 4046 2472 Size: 1574 4343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI