(Circular) (Six-base) MAPSORT of: pFN21AB8683.seq Check: 5095 from: 1 to: 6721 pFN21AB8683 6721 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 2938 4042 2938 Size: 1104 5617 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 2114 2198 2225 2870 2895 2927 4784 Size: 463 19 84 27 645 25 32 1857 Cuts at: 4784 6168 6436 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1857 1384 645 463 268 84 32 27 25 19 AflII C'TTAA_G Cuts at: 820 1017 2446 820 Size: 197 1429 5095 Fragments arranged by size: 5095 1429 197 AflIII A'CryG_T Cuts at: 1203 3147 5620 6597 1203 Size: 1944 2473 977 1327 Fragments arranged by size: 2473 1944 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 3364 5450 3364 Size: 2086 4635 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3333 1184 Size: 2149 4572 AlwNI CAG_nnn'CTG Cuts at: 2575 4464 6036 2575 Size: 1889 1572 3260 Fragments arranged by size: 3260 1889 1572 ApaLI G'TGCA_C Cuts at: 4778 5934 4778 Size: 1156 5565 ApoI r'AATT_y Cuts at: 1493 2087 2348 3128 3834 3881 4030 4256 4408 Size: 594 261 780 706 47 149 226 152 Cuts at: 4408 6517 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 780 706 594 261 226 152 149 47 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 3599 4046 1870 Size: 73 1656 447 4545 Fragments arranged by size: 4545 1656 447 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3454 3487 3454 Size: 33 6688 BamHI G'GATC_C Cuts at: 4051 4418 4051 Size: 367 6354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2642 2938 4042 5497 618 Size: 325 217 627 855 296 1104 1455 1842 Fragments arranged by size: 1842 1455 1104 855 627 325 296 217 BanII G_rGCy'C Cuts at: 1391 2529 2586 3091 3600 3689 4040 1391 Size: 1138 57 505 509 89 351 4072 Fragments arranged by size: 4072 1138 509 505 351 89 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 2634 2876 3197 928 Size: 971 665 70 242 321 4452 Fragments arranged by size: 4452 971 665 321 242 70 BbvCI CC'TCA_GC Cuts at: 2214 2333 2487 2214 Size: 119 154 6448 Fragments arranged by size: 6448 154 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2695 4613 5823 2695 Size: 1918 1210 3593 Fragments arranged by size: 3593 1918 1210 BclI T'GATC_A Cuts at: 1548 2634 1548 Size: 1086 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3248 5332 136 Size: 107 122 71 631 605 1576 2084 1525 Fragments arranged by size: 2084 1576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6716 6716 Size: 6721 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 4782 5938 1790 Size: 2992 1156 2573 Fragments arranged by size: 2992 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3959 4452 5410 Size: 416 53 83 399 24 2542 493 958 Cuts at: 5410 442 Size: 1753 Fragments arranged by size: 2542 1753 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2441 2740 2858 3065 3206 3416 5381 Size: 971 231 299 118 207 141 210 1965 Cuts at: 5381 1239 Size: 2579 Fragments arranged by size: 2579 1965 971 299 231 210 207 141 118 Bpu10I CC'TnA_GC Cuts at: 2214 2333 2487 2214 Size: 119 154 6448 Fragments arranged by size: 6448 154 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 2813 4846 5711 6009 2531 Size: 282 2033 865 298 3243 Fragments arranged by size: 3243 2033 865 298 282 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 5384 882 Size: 617 519 3366 2219 Fragments arranged by size: 3366 2219 617 519 BsaAI yAC'GTr Cuts at: 493 1204 2590 3150 3502 4512 493 Size: 711 1386 560 352 1010 2702 Fragments arranged by size: 2702 1386 1010 711 560 352 BsaBI GATnn'nnATC Cuts at: 6715 6715 Size: 6721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4528 4910 5545 275 Size: 53 83 186 936 2995 382 635 1451 Fragments arranged by size: 2995 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2757 5154 5552 5826 5973 6384 2757 Size: 2397 398 274 147 411 3094 Fragments arranged by size: 3094 2397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6496 Fragments arranged by size: 6496 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3302 3595 1186 Size: 2116 293 4312 Fragments arranged by size: 4312 2116 293 BseYI C'CCAG_C Cuts at: 2236 2475 3001 3273 3688 5924 6420 2236 Size: 239 526 272 415 2236 496 2537 Fragments arranged by size: 2537 2236 526 496 415 272 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6148 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 4171 4932 5960 1540 Size: 275 186 1216 954 761 1028 2301 Fragments arranged by size: 2301 1216 1028 954 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 4782 4867 5938 2586 Size: 2196 85 1071 3369 Fragments arranged by size: 3369 2196 1071 85 BsmI GAATG_Cn' Cuts at: 2361 2992 3322 4232 4325 2361 Size: 631 330 910 93 4757 Fragments arranged by size: 4757 910 631 330 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 2586 3091 3600 3689 4040 4782 Size: 399 739 57 505 509 89 351 742 Cuts at: 4782 4867 5938 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 509 505 399 351 89 85 57 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 2679 4610 6340 2679 Size: 1931 1730 3060 Fragments arranged by size: 3060 1931 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4076 844 Size: 3232 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2752 4608 1394 Size: 429 245 684 1856 3507 Fragments arranged by size: 3507 1856 684 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 5216 5390 65 Size: 1183 3968 174 1396 Fragments arranged by size: 3968 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 4540 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 3206 4781 5793 3206 Size: 1575 1012 4134 Fragments arranged by size: 4134 1575 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6721 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 2419 3260 3328 3502 2419 Size: 841 68 174 5638 Fragments arranged by size: 5638 841 174 68 BstYI r'GATC_y Cuts at: 1972 2198 3162 4051 4418 4803 4820 6272 6358 Size: 226 964 889 367 385 17 1452 86 Cuts at: 6358 6370 6716 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 964 889 385 367 346 226 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2283 513 Size: 552 1218 4951 Fragments arranged by size: 4951 1218 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 2875 3284 3623 4237 5031 5051 2875 Size: 409 339 614 794 20 4545 Fragments arranged by size: 4545 794 614 409 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 4025 4025 Size: 6721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3214 4168 Size: 54 1425 50 275 18 225 1159 954 Cuts at: 4168 5057 6686 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1159 954 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 4168 1537 Size: 275 1402 954 4090 Fragments arranged by size: 4090 1402 954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 3814 3928 4651 1353 Size: 1575 886 114 723 3423 Fragments arranged by size: 3423 1575 886 723 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2555 5308 5682 5828 387 Size: 1064 138 966 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 966 374 146 138 EcoICRI GAG'CTC Cuts at: 2584 2584 Size: 6721 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2114 2198 2210 2225 2441 2740 Size: 393 463 19 84 12 15 216 299 Cuts at: 2740 2858 2870 2895 2927 3065 3206 3416 4784 Size: 118 12 25 32 138 141 210 1368 Cuts at: 4784 5381 6168 6436 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1368 787 597 463 393 299 268 216 210 141 138 118 84 32 25 19 15 12 12 EcoO109I rG'GnC_Cy Cuts at: 2434 2671 3040 3391 3754 2434 Size: 237 369 351 363 5401 Fragments arranged by size: 5401 369 363 351 237 EcoRI G'AATT_C Cuts at: 2348 3881 4030 4408 2348 Size: 1533 149 378 4661 Fragments arranged by size: 4661 1533 378 149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2424 2900 2424 Size: 476 6245 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 5868 1678 Size: 4190 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 3065 3097 3350 Size: 32 135 32 754 32 707 32 253 Cuts at: 3350 3382 5362 5394 5436 5468 1373 Size: 32 1980 32 42 32 2626 Fragments arranged by size: 2626 1980 754 707 253 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4065 4311 669 Size: 988 281 2127 246 3079 Fragments arranged by size: 3079 2127 988 281 246 HindIII A'AGCT_T Cuts at: 748 3407 3534 748 Size: 2659 127 3935 Fragments arranged by size: 3935 2659 127 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 2942 4046 2942 Size: 1104 5617 MfeI C'AATT_G Cuts at: 4320 4320 Size: 6721 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2941 5835 6019 1305 Size: 684 952 2894 184 2007 Fragments arranged by size: 2894 2007 952 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4899 Fragments arranged by size: 4899 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2849 4679 5038 5197 518 Size: 648 1683 1830 359 159 2042 Fragments arranged by size: 2042 1830 1683 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3219 4137 4814 5962 6207 1606 Size: 1613 918 677 1148 245 2120 Fragments arranged by size: 2120 1613 1148 918 677 245 NcoI C'CATG_G Cuts at: 513 1065 2283 513 Size: 552 1218 4951 Fragments arranged by size: 4951 1218 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6721 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 4079 5624 1132 Size: 97 2850 1545 2229 Fragments arranged by size: 2850 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 1108 Size: 1467 5254 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PmlI CAC'GTG Cuts at: 3150 3150 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4790 4822 6303 6335 4790 Size: 32 1481 32 5176 Fragments arranged by size: 5176 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 2671 3040 2434 Size: 237 369 6115 Fragments arranged by size: 6115 369 237 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6721 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PstI C_TGCA'G Cuts at: 830 3027 3684 4073 830 Size: 2197 657 389 3478 Fragments arranged by size: 3478 2197 657 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 SacI G_AGCT'C Cuts at: 2586 2586 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SfcI C'TryA_G Cuts at: 826 1045 3023 3680 4069 5204 5885 6076 826 Size: 219 1978 657 389 1135 681 191 1471 Fragments arranged by size: 1978 1471 1135 681 657 389 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3248 3248 Size: 6721 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 4048 1872 Size: 2176 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2446 2546 2828 4825 5726 5988 Size: 197 926 503 100 282 1997 901 262 Cuts at: 5988 820 Size: 1553 Fragments arranged by size: 1997 1553 926 901 503 282 262 197 100 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 4079 1132 Size: 2947 3774 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 2338 2540 3242 3847 1517 Size: 821 202 702 605 4391 Fragments arranged by size: 4391 821 702 605 202 StyI C'CwwG_G Cuts at: 513 1065 2283 2304 2517 3054 3450 3750 4160 Size: 552 1218 21 213 537 396 300 410 Cuts at: 4160 513 Size: 3074 Fragments arranged by size: 3074 1218 552 537 410 396 300 213 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4748 4765 4918 5103 5548 325 Size: 1255 3168 17 153 185 445 1498 Fragments arranged by size: 3168 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 4967 6679 Size: 274 80 33 51 1443 300 2690 1712 Cuts at: 6679 96 Size: 138 Fragments arranged by size: 2690 1712 1443 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 6681 6713 3290 Size: 32 3359 32 3298 Fragments arranged by size: 3359 3298 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 4057 4057 Size: 6721 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3328 3733 3328 Size: 405 6316 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 4046 1870 Size: 2176 4545 XmnI GAAnn'nnTTC Cuts at: 2404 4850 2404 Size: 2446 4275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI