(Circular) (Six-base) MAPSORT of: pFN21AB8680.seq Check: 4909 from: 1 to: 6604 pFN21AB8680 6604 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3813 4414 278 Size: 53 83 186 936 2277 601 2468 Fragments arranged by size: 2468 2277 936 601 186 83 53 AccI GT'mk_AC Cuts at: 1937 2880 3947 1937 Size: 943 1067 4594 Fragments arranged by size: 4594 1067 943 Acc65I G'GTAC_C Cuts at: 3925 3925 Size: 6604 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 3033 3267 3717 4667 6051 6319 1632 Size: 1143 258 234 450 950 1384 268 1917 Fragments arranged by size: 1917 1384 1143 950 450 268 258 234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 3078 3583 3901 5503 6480 1203 Size: 1875 505 318 1602 977 1327 Fragments arranged by size: 1875 1602 1327 977 505 318 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 2823 1166 Size: 1657 4947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3242 3467 1184 Size: 2058 225 4321 Fragments arranged by size: 4321 2058 225 AlwNI CAG_nnn'CTG Cuts at: 2141 4347 5919 2141 Size: 2206 1572 2826 Fragments arranged by size: 2826 2206 1572 ApaLI G'TGCA_C Cuts at: 2079 4661 5817 2079 Size: 2582 1156 2866 Fragments arranged by size: 2866 2582 1156 ApoI r'AATT_y Cuts at: 1493 3913 4139 4291 6400 1493 Size: 2420 226 152 2109 1697 Fragments arranged by size: 2420 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5158 160 Size: 872 4126 1606 Fragments arranged by size: 4126 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 2429 3929 1870 Size: 73 486 1500 4545 Fragments arranged by size: 4545 1500 486 73 BamHI G'GATC_C Cuts at: 3934 4301 3934 Size: 367 6237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3864 3925 5380 618 Size: 325 217 627 2077 61 1455 1842 Fragments arranged by size: 2077 1842 1455 627 325 217 61 BanII G_rGCy'C Cuts at: 1391 2430 2448 3923 1391 Size: 1039 18 1475 4072 Fragments arranged by size: 4072 1475 1039 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2361 2877 3420 928 Size: 971 462 516 543 4112 Fragments arranged by size: 4112 971 543 516 462 BbvCI CC'TCA_GC Cuts at: 2384 2439 2619 2384 Size: 55 180 6369 Fragments arranged by size: 6369 180 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4795 4829 1903 Size: 34 50 34 2774 34 3678 Fragments arranged by size: 3678 2774 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4496 5706 4496 Size: 1210 5394 BclI T'GATC_A Cuts at: 1548 2956 3172 1548 Size: 1408 216 4980 Fragments arranged by size: 4980 1408 216 BfrBI ATG'CAT Cuts at: 2934 2934 Size: 6604 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5215 136 Size: 107 122 71 631 605 3543 1525 Fragments arranged by size: 3543 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6599 6599 Size: 6604 BlpI GC'TnA_GC Cuts at: 2657 3215 3440 4021 2657 Size: 558 225 581 5240 Fragments arranged by size: 5240 581 558 225 Bme1580I G_kGCm'C Cuts at: 1790 2083 4665 5821 1790 Size: 293 2582 1156 2573 Fragments arranged by size: 2582 2573 1156 293 BmgBI CAC'GTC Cuts at: 1318 3604 3829 1318 Size: 2286 225 4093 Fragments arranged by size: 4093 2286 225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2488 4335 5293 Size: 416 53 83 399 24 1071 1847 958 Cuts at: 5293 442 Size: 1753 Fragments arranged by size: 1847 1753 1071 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2804 5264 1239 Size: 1298 267 2460 2579 Fragments arranged by size: 2579 2460 1298 267 Bpu10I CC'TnA_GC Cuts at: 2384 2439 2447 2619 2384 Size: 55 8 172 6369 Fragments arranged by size: 6369 172 55 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2292 4729 5594 5892 2292 Size: 2437 865 298 3004 Fragments arranged by size: 3004 2437 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5267 882 Size: 617 3768 2219 Fragments arranged by size: 3768 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4395 493 Size: 711 3191 2702 Fragments arranged by size: 3191 2702 711 BsaBI GATnn'nnATC Cuts at: 6598 6598 Size: 6604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3810 4411 4793 5428 Size: 53 83 186 936 2277 601 382 635 Cuts at: 5428 275 Size: 1451 Fragments arranged by size: 2277 1451 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2198 2569 5037 5435 5709 5856 6267 2198 Size: 371 2468 398 274 147 411 2535 Fragments arranged by size: 2535 2468 411 398 371 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2586 2616 1178 Size: 30 165 30 1183 30 5166 Fragments arranged by size: 5166 1183 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2456 2552 2717 2801 3032 1186 Size: 1252 18 96 165 84 231 4758 Fragments arranged by size: 4758 1252 231 165 96 84 18 BseYI C'CCAG_C Cuts at: 2250 5807 6303 2250 Size: 3557 496 2551 Fragments arranged by size: 3557 2551 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2765 1163 Size: 573 1029 5002 Fragments arranged by size: 5002 1029 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4815 5843 1540 Size: 275 186 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2083 2430 2448 3110 4665 4750 5821 2083 Size: 347 18 662 1555 85 1071 2866 Fragments arranged by size: 2866 1555 1071 662 347 85 18 BsmI GAATG_Cn' Cuts at: 4115 4208 4115 Size: 93 6511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2430 2448 3110 3923 4665 4750 Size: 399 293 347 18 662 813 742 85 Cuts at: 4750 5821 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 813 742 662 399 347 293 85 18 BspEI T'CCGG_A Cuts at: 2569 6267 2569 Size: 3698 2906 BspHI T'CATG_A Cuts at: 2908 2959 4493 6223 2908 Size: 51 1534 1730 3289 Fragments arranged by size: 3289 1730 1534 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 844 Size: 3115 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2395 2535 4491 1394 Size: 429 572 140 1956 3507 Fragments arranged by size: 3507 1956 572 429 140 BsrDI GCAATG_nn' Cuts at: 65 1248 3344 5099 5273 65 Size: 1183 2096 1755 174 1396 Fragments arranged by size: 2096 1755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 2331 5248 5435 1700 Size: 110 376 145 2917 187 2869 Fragments arranged by size: 2917 2869 376 187 145 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6604 BstBI TT'CG_AA Cuts at: 5408 5408 Size: 6604 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6604 BstYI r'GATC_y Cuts at: 1972 2128 3934 4301 4686 4703 6155 6241 6253 Size: 156 1806 367 385 17 1452 86 12 Cuts at: 6253 6599 1972 Size: 346 1977 Fragments arranged by size: 1977 1806 1452 385 367 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2772 3471 2772 Size: 699 5905 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6052 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5865 Fragments arranged by size: 5865 683 56 BtsI GCAGTG_nn' Cuts at: 4120 4914 4934 4120 Size: 794 20 5790 Fragments arranged by size: 5790 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2804 2839 529 Size: 35 2240 35 4294 Fragments arranged by size: 4294 2240 35 35 DraI TTT'AAA Cuts at: 3908 3908 Size: 6604 DraIII CAC_nnn'GTG Cuts at: 2102 2102 Size: 6604 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5611 809 Size: 507 4295 1802 Fragments arranged by size: 4295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 4051 4940 Size: 54 1425 50 275 18 495 1726 889 Cuts at: 4940 6569 8 Size: 1629 43 Fragments arranged by size: 1726 1629 1425 889 495 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 1537 Size: 275 2239 4090 Fragments arranged by size: 4090 2239 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2158 2403 3009 3399 3684 4534 1353 Size: 805 245 606 390 285 850 3423 Fragments arranged by size: 3423 850 805 606 390 285 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5191 5565 5711 387 Size: 1064 138 3602 374 146 1280 Fragments arranged by size: 3602 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2428 2446 2428 Size: 18 6586 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2775 2804 3033 3267 3717 4667 Size: 393 905 238 29 229 234 450 950 Cuts at: 4667 5264 6051 6319 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 950 905 787 597 450 393 268 238 234 229 29 EcoO109I rG'GnC_Cy Cuts at: 2051 2557 2599 2051 Size: 506 42 6056 Fragments arranged by size: 6056 506 42 EcoRI G'AATT_C Cuts at: 3913 4291 3913 Size: 378 6226 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2432 2432 Size: 6604 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 5751 1678 Size: 4073 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2586 2618 2888 2920 3326 Size: 32 135 32 1014 32 270 32 406 Cuts at: 3326 3358 5245 5277 5319 5351 1373 Size: 32 1887 32 42 32 2626 Fragments arranged by size: 2626 1887 1014 406 270 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2881 3948 4194 669 Size: 988 281 943 1067 246 3079 Fragments arranged by size: 3079 1067 988 943 281 246 HindIII A'AGCT_T Cuts at: 748 2414 748 Size: 1666 4938 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3929 3929 Size: 6604 MfeI C'AATT_G Cuts at: 4203 4203 Size: 6604 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2156 2856 2901 5718 5902 1305 Size: 851 700 45 2817 184 2007 Fragments arranged by size: 2817 2007 851 700 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 4287 Fragments arranged by size: 4287 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2823 3168 4562 4921 5080 518 Size: 648 1657 345 1394 359 159 2042 Fragments arranged by size: 2042 1657 1394 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 2254 2614 4020 4697 5845 6090 1606 Size: 648 360 1406 677 1148 245 2120 Fragments arranged by size: 2120 1406 1148 677 648 360 245 NaeI GCC'GGC Cuts at: 2188 2188 Size: 6604 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6052 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NgoMIV G'CCGG_C Cuts at: 2186 2186 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NsiI A_TGCA'T Cuts at: 2936 2936 Size: 6604 NspI r_CATG'y Cuts at: 1132 1229 3082 3761 3962 5507 1132 Size: 97 1853 679 201 1545 2229 Fragments arranged by size: 2229 1853 1545 679 201 97 PasI CC'CwG_GG Cuts at: 1348 2590 2736 1348 Size: 1242 146 5216 Fragments arranged by size: 5216 1242 146 PciI A'CATG_T Cuts at: 3078 5503 3078 Size: 2425 4179 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6604 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 2599 Size: 6604 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 4174 4174 Size: 6604 PstI C_TGCA'G Cuts at: 830 3006 3956 830 Size: 2176 950 3478 Fragments arranged by size: 3478 2176 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 2254 2614 2254 Size: 360 6244 SacI G_AGCT'C Cuts at: 2430 2448 2430 Size: 18 6586 SalI G'TCGA_C Cuts at: 1936 2879 3946 1936 Size: 943 1067 4594 Fragments arranged by size: 4594 1067 943 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 6604 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 4852 6564 4852 Size: 1712 4892 SfcI C'TryA_G Cuts at: 826 1045 3002 3325 3952 5087 5768 5959 826 Size: 219 1957 323 627 1135 681 191 1471 Fragments arranged by size: 1957 1471 1135 681 627 323 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3931 1872 Size: 2059 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 4708 5609 5871 820 Size: 197 926 364 2401 901 262 1553 Fragments arranged by size: 2401 1553 926 901 364 262 197 SnaBI TAC'GTA Cuts at: 493 4395 493 Size: 3902 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6604 SphI G_CATG'C Cuts at: 1132 3962 1132 Size: 2830 3774 SspI AAT'ATT Cuts at: 5 52 4528 5 Size: 47 4476 2081 Fragments arranged by size: 4476 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6604 StyI C'CwwG_G Cuts at: 513 1065 2192 3361 3418 4043 513 Size: 552 1127 1169 57 625 3074 Fragments arranged by size: 3074 1169 1127 625 552 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4631 4648 4801 4986 5431 325 Size: 1255 3051 17 153 185 445 1498 Fragments arranged by size: 3051 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2716 3774 4850 Size: 274 80 33 51 1443 739 1058 1076 Cuts at: 4850 6562 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1076 1058 739 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6564 6596 6564 Size: 32 6572 Tth111I GACn'n_nGTC Cuts at: 1316 2876 1316 Size: 1560 5044 XbaI T'CTAG_A Cuts at: 2224 3940 2224 Size: 1716 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6604 XmaI C'CCGG_G Cuts at: 1870 3929 1870 Size: 2059 4545 XmnI GAAnn'nnTTC Cuts at: 4733 4733 Size: 6604 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3811 4412 276 Size: 53 83 186 936 2277 601 2468 Fragments arranged by size: 2468 2277 936 601 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI