Restriction map for
pFN21AB8678
(Linear) (Six-base) MAPSORT of: KIBB8678.seq Check: 2289 from: 1 to: 1128
KIBB8678.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AatII G_ACGT'C
Cuts at: 0 569 1128
Size: 569 559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1128
Size: 771 357
AleI CACnn'nnGTG
Cuts at: 0 134 463 1128
Size: 134 329 665
Fragments arranged by size:
665 329 134
ApaI G_GGCC'C
Cuts at: 0 774 1128
Size: 774 354
BamHI G'GATC_C
Cuts at: 0 49 1128
Size: 49 1079
BanI G'GyrC_C
Cuts at: 0 336 1099 1128
Size: 336 763 29
Fragments arranged by size:
763 336 29
BanII G_rGCy'C
Cuts at: 0 153 774 1033 1128
Size: 153 621 259 95
Fragments arranged by size:
621 259 153 95
BbeI G_GCGC'C
Cuts at: 0 340 1128
Size: 340 788
BbsI GAAGACnn'nnnn_
Cuts at: 0 1100 1128
Size: 1100 28
BbvCI CC'TCA_GC
Cuts at: 0 185 1128
Size: 185 943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 534 568 1128
Size: 534 34 560
Fragments arranged by size:
560 534 34
BclI T'GATC_A
Cuts at: 0 81 1128
Size: 81 1047
Bme1580I G_kGCm'C
Cuts at: 0 404 506 774 1104 1128
Size: 404 102 268 330 24
Fragments arranged by size:
404 330 268 102 24
Bpu10I CC'TnA_GC
Cuts at: 0 185 1128
Size: 185 943
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 1077 1128
Size: 382 695 51
Fragments arranged by size:
695 382 51
BsaAI yAC'GTr
Cuts at: 0 1019 1128
Size: 1019 109
BsaHI Gr'CG_yC
Cuts at: 0 337 566 1128
Size: 337 229 562
Fragments arranged by size:
562 337 229
BsaWI w'CCGG_w
Cuts at: 0 52 1128
Size: 52 1076
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 994 1024 1128
Size: 994 30 104
Fragments arranged by size:
994 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 325 823 1128
Size: 202 123 498 305
Fragments arranged by size:
498 305 202 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1128
Size: 977 151
BsiHKAI G_wGCw'C
Cuts at: 0 194 326 1128
Size: 194 132 802
Fragments arranged by size:
802 194 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 688 1128
Size: 573 115 440
Fragments arranged by size:
573 440 115
Bsp1286I G_dGCh'C
Cuts at: 0 153 194 326 404 506 774 1033 1104
Size: 153 41 132 78 102 268 259 71
Cuts at: 1104 1128
Size: 24
Fragments arranged by size:
268 259 153 132 102 78 71 41
24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 734 875 1128
Size: 599 135 141 253
Fragments arranged by size:
599 253 141 135
BsrBI CCG'CTC
Cuts at: 0 406 1128
Size: 406 722
BssSI C'ACGA_G
Cuts at: 0 826 1128
Size: 826 302
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 1128
Size: 321 807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1128
Size: 465 663
BstYI r'GATC_y
Cuts at: 0 49 86 1128
Size: 49 37 1042
Fragments arranged by size:
1042 49 37
Bsu36I CC'TnA_GG
Cuts at: 0 998 1128
Size: 998 130
BtgI C'CryG_G
Cuts at: 0 221 1128
Size: 221 907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 537 1128
Size: 537 591
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 95 130 1005 1040 1128
Size: 95 35 875 35 88
Fragments arranged by size:
875 95 88 35 35
DraI TTT'AAA
Cuts at: 0 344 1128
Size: 344 784
EarI CTCTTCn'nnn_
Cuts at: 0 790 1128
Size: 790 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1128
Size: 771 357
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 595 1128
Size: 331 264 533
Fragments arranged by size:
533 331 264
EcoO109I rG'GnC_Cy
Cuts at: 0 771 831 1128
Size: 771 60 297
Fragments arranged by size:
771 297 60
EcoRV GAT'ATC
Cuts at: 0 989 1128
Size: 989 139
FspI TGC'GCA
Cuts at: 0 544 1128
Size: 544 584
FspAI rTGC'GCAy
Cuts at: 0 544 1128
Size: 544 584
HaeII r_GCGC'y
Cuts at: 0 340 1128
Size: 340 788
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 601 633 1128
Size: 34 32 535 32 495
Fragments arranged by size:
535 495 34 32 32
KasI G'GCGC_C
Cuts at: 0 336 1128
Size: 336 792
MslI CAynn'nnrTG
Cuts at: 0 134 418 463 1128
Size: 134 284 45 665
Fragments arranged by size:
665 284 134 45
MspA1I CmG'CkG
Cuts at: 0 294 1128
Size: 294 834
NarI GG'CG_CC
Cuts at: 0 337 1128
Size: 337 791
NcoI C'CATG_G
Cuts at: 0 221 1128
Size: 221 907
PmlI CAC'GTG
Cuts at: 0 1019 1128
Size: 1019 109
PpuMI rG'GwC_Cy
Cuts at: 0 831 1128
Size: 831 297
PspOMI G'GGCC_C
Cuts at: 0 770 1128
Size: 770 358
PstI C_TGCA'G
Cuts at: 0 299 1128
Size: 299 829
PvuII CAG'CTG
Cuts at: 0 294 1128
Size: 294 834
SapI GCTCTTCn'nnn_
Cuts at: 0 790 1128
Size: 790 338
ScaI AGT'ACT
Cuts at: 0 721 1128
Size: 721 407
SfcI C'TryA_G
Cuts at: 0 295 446 1128
Size: 295 151 682
Fragments arranged by size:
682 295 151
SfoI GGC'GCC
Cuts at: 0 338 1128
Size: 338 790
StyI C'CwwG_G
Cuts at: 0 221 1128
Size: 221 907
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 1128
Size: 508 620
TatI w'GTAC_w
Cuts at: 0 429 719 1128
Size: 429 290 409
Fragments arranged by size:
429 409 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 41 73 1128
Size: 41 32 1055
Fragments arranged by size:
1055 41 32
ZraI GAC'GTC
Cuts at: 0 567 1128
Size: 567 561
Enzymes that do cut:
AatII AcuI AleI ApaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI Bme1580I Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI CspCI DraI EarI Eco57MI EcoNI EcoO109I EcoRV
FspI FspAI HaeII Hin4I KasI MslI MspA1I NarI
NcoI PmlI PpuMI PspOMI PstI PvuII SapI ScaI
SfcI SfoI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaBI
BseYI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I BtsI ClaI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRI FalI FseI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI