(Circular) (Six-base) MAPSORT of: pFN21AB8676.seq Check: 4305 from: 1 to: 5971 pFN21AB8676 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2314 2314 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3202 3292 3202 Size: 90 5881 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2367 3009 4034 5418 5686 1632 Size: 735 642 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1025 735 642 268 AflII C'TTAA_G Cuts at: 820 1017 2264 820 Size: 197 1247 4527 Fragments arranged by size: 4527 1247 197 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2883 2915 2883 Size: 32 5939 AlwNI CAG_nnn'CTG Cuts at: 3054 3714 5286 3054 Size: 660 1572 3739 Fragments arranged by size: 3739 1572 660 ApaI G_GGCC'C Cuts at: 2385 2385 Size: 5971 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2723 3280 3506 3658 5767 1493 Size: 1230 557 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 557 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2838 3296 1870 Size: 73 895 458 4545 Fragments arranged by size: 4545 895 458 73 AvrII C'CTAG_G Cuts at: 2213 2213 Size: 5971 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2966 2999 2966 Size: 33 5938 BamHI G'GATC_C Cuts at: 2943 3301 3668 2943 Size: 358 367 5246 Fragments arranged by size: 5246 367 358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 3202 3292 4747 618 Size: 325 217 627 539 876 90 1455 1842 Fragments arranged by size: 1842 1455 876 627 539 325 217 90 BanII G_rGCy'C Cuts at: 1391 2385 2469 3290 1391 Size: 994 84 821 4072 Fragments arranged by size: 4072 994 821 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2651 928 Size: 971 752 4248 Fragments arranged by size: 4248 971 752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2994 3863 5073 2994 Size: 869 1210 3892 Fragments arranged by size: 3892 1210 869 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2316 2799 3388 2316 Size: 483 589 4899 Fragments arranged by size: 4899 589 483 Bme1580I G_kGCm'C Cuts at: 1790 2385 4032 5188 1790 Size: 595 1647 1156 2573 Fragments arranged by size: 2573 1647 1156 595 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2418 3042 3702 Size: 416 53 83 399 24 1001 624 660 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 1001 958 660 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2916 1056 Size: 1860 4111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 4631 1239 Size: 1136 2256 2579 Fragments arranged by size: 2579 2256 1136 Bpu10I CC'TnA_GC Cuts at: 2222 2222 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2172 2426 2858 4096 4961 5259 2172 Size: 254 432 1238 865 298 2884 Fragments arranged by size: 2884 1238 865 432 298 254 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2899 3037 4634 882 Size: 617 1400 138 1597 2219 Fragments arranged by size: 2219 1597 1400 617 138 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2007 2037 2057 2087 2235 Size: 30 165 30 604 30 20 30 148 Cuts at: 2235 2265 2883 2913 1178 Size: 30 618 30 4236 Fragments arranged by size: 4236 618 604 165 148 30 30 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2842 1186 Size: 1656 4315 BseYI C'CCAG_C Cuts at: 2226 2462 5174 5670 2226 Size: 236 2712 496 2527 Fragments arranged by size: 2712 2527 496 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2539 2558 3040 1163 Size: 573 803 19 482 4094 Fragments arranged by size: 4094 803 573 482 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2977 4032 4117 5188 2977 Size: 1055 85 1071 3760 Fragments arranged by size: 3760 1071 1055 85 BsmI GAATG_Cn' Cuts at: 2036 3482 3575 2036 Size: 1446 93 4432 Fragments arranged by size: 4432 1446 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2469 2977 3290 4032 4117 5188 Size: 399 595 84 508 313 742 85 1071 Cuts at: 5188 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 595 508 399 313 85 84 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3036 3078 3860 5590 3036 Size: 42 782 1730 3417 Fragments arranged by size: 3417 1730 782 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 2616 2695 2917 3326 844 Size: 1470 302 79 222 409 3489 Fragments arranged by size: 3489 1470 409 302 222 79 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 4466 4640 65 Size: 1183 945 2273 174 1396 Fragments arranged by size: 2273 1396 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3227 4615 4802 1700 Size: 110 1417 1388 187 2869 Fragments arranged by size: 2869 1417 1388 187 110 BsrGI T'GTAC_A Cuts at: 96 3069 96 Size: 2973 2998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2943 3301 3668 4053 4070 5522 5608 5620 Size: 971 358 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 971 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 2330 2749 513 Size: 552 939 273 53 419 3735 Fragments arranged by size: 3735 939 552 419 273 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2374 2793 3487 4281 4301 2374 Size: 419 694 794 20 4044 Fragments arranged by size: 4044 794 694 419 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 2451 3275 2451 Size: 824 5147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3225 3418 4307 Size: 54 1425 50 275 18 1395 193 889 Cuts at: 4307 5936 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1395 889 275 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3035 3254 3901 1353 Size: 1682 219 647 3423 Fragments arranged by size: 3423 1682 647 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2367 2375 3009 4034 4631 5418 5686 Size: 393 735 8 634 1025 597 787 268 Cuts at: 5686 1239 Size: 1524 Fragments arranged by size: 1524 1025 787 735 634 597 393 268 8 EcoO109I rG'GnC_Cy Cuts at: 2218 2413 2605 2707 2218 Size: 195 192 102 5482 Fragments arranged by size: 5482 195 192 102 EcoRI G'AATT_C Cuts at: 2723 3280 3658 2723 Size: 557 378 5036 Fragments arranged by size: 5036 557 378 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2720 3097 5118 1678 Size: 1042 377 2021 2531 Fragments arranged by size: 2531 2021 1042 377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2832 2864 2913 2918 2945 Size: 32 135 32 1260 32 49 5 27 Cuts at: 2945 2950 2982 3101 3133 4612 4644 4686 4718 Size: 5 32 119 32 1479 32 42 32 Cuts at: 4718 1373 Size: 2626 Fragments arranged by size: 2626 1479 1260 135 119 49 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3206 3296 3206 Size: 90 5881 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 5085 5269 1305 Size: 1260 2520 184 2007 Fragments arranged by size: 2520 2007 1260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2754 3236 3929 4288 4447 518 Size: 648 843 745 482 693 359 159 2042 Fragments arranged by size: 2042 843 745 693 648 482 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 2462 2542 3221 3387 4064 5212 5457 Size: 726 130 80 679 166 677 1148 245 Cuts at: 5457 1606 Size: 2120 Fragments arranged by size: 2120 1148 726 679 677 245 166 130 80 NaeI GCC'GGC Cuts at: 3229 3229 Size: 5971 NcoI C'CATG_G Cuts at: 513 1065 2004 2749 513 Size: 552 939 745 3735 Fragments arranged by size: 3735 939 745 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NgoMIV G'CCGG_C Cuts at: 3227 3227 Size: 5971 NheI G'CTAG_C Cuts at: 1052 2912 1052 Size: 1860 4111 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3268 3329 4874 1132 Size: 97 2039 61 1545 2229 Fragments arranged by size: 2229 2039 1545 97 61 PasI CC'CwG_GG Cuts at: 1348 2599 1348 Size: 1251 4720 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PfoI T'CCnGG_A Cuts at: 2025 2061 2025 Size: 36 5935 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2883 2915 4040 4072 5553 5585 2883 Size: 32 1125 32 1481 32 3269 Fragments arranged by size: 3269 1481 1125 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2413 2605 2707 2218 Size: 195 192 102 5482 Fragments arranged by size: 5482 195 192 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2381 2381 Size: 5971 PstI C_TGCA'G Cuts at: 830 2325 2733 3166 3323 830 Size: 1495 408 433 157 3478 Fragments arranged by size: 3478 1495 433 408 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2462 2542 3221 2462 Size: 80 679 5212 Fragments arranged by size: 5212 679 80 SacII CC_GC'GG Cuts at: 2333 2333 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SanDI GG'GwC_CC Cuts at: 2413 2413 Size: 5971 SbfI CC_TGCA'GG Cuts at: 2733 3323 2733 Size: 590 5381 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SexAI A'CCwGG_T Cuts at: 2532 2532 Size: 5971 SfcI C'TryA_G Cuts at: 826 1045 2321 2729 3162 3259 3319 4454 5135 Size: 219 1276 408 433 97 60 1135 681 Cuts at: 5135 5326 826 Size: 191 1471 Fragments arranged by size: 1471 1276 1135 681 433 408 219 191 97 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2187 2264 2441 2873 4075 4976 Size: 197 926 244 77 177 432 1202 901 Cuts at: 4976 5238 820 Size: 262 1553 Fragments arranged by size: 1553 1202 926 901 432 262 244 197 177 77 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3268 3329 1132 Size: 2136 61 3774 Fragments arranged by size: 3774 2136 61 SspI AAT'ATT Cuts at: 5 52 2773 3895 5 Size: 47 2721 1122 2081 Fragments arranged by size: 2721 2081 1122 47 StuI AGG'CCT Cuts at: 1517 2817 1517 Size: 1300 4671 StyI C'CwwG_G Cuts at: 513 1065 2004 2213 2749 3410 513 Size: 552 939 209 536 661 3074 Fragments arranged by size: 3074 939 661 552 536 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3069 4217 5929 Size: 274 80 33 51 1443 1092 1148 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1712 1443 1148 1092 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 2235 2267 5931 5963 2007 Size: 32 196 32 3664 32 2015 Fragments arranged by size: 3664 2015 196 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 2308 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2286 2895 4100 2286 Size: 609 1205 4157 Fragments arranged by size: 4157 1205 609 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI