(Circular) (Six-base) MAPSORT of: pFN21AB8675.seq Check: 4418 from: 1 to: 6571 pFN21AB8675 6571 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2796 2287 Size: 509 6062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2481 2699 4381 278 Size: 53 83 186 936 945 218 1682 2468 Fragments arranged by size: 2468 1682 945 936 218 186 83 53 AccI GT'mk_AC Cuts at: 1937 3333 3914 1937 Size: 1396 581 4594 Fragments arranged by size: 4594 1396 581 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3155 4634 6018 6286 1632 Size: 1523 1479 1384 268 1917 Fragments arranged by size: 1917 1523 1479 1384 268 AfeI AGC'GCT Cuts at: 2174 2174 Size: 6571 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 3695 1184 Size: 1017 1494 4060 Fragments arranged by size: 4060 1494 1017 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 2489 Size: 32 6539 AlwNI CAG_nnn'CTG Cuts at: 2086 2287 2329 2362 4314 5886 2086 Size: 201 42 33 1952 1572 2771 Fragments arranged by size: 2771 1952 1572 201 42 33 ApaI G_GGCC'C Cuts at: 3084 3376 3084 Size: 292 6279 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3880 4106 4258 6367 1493 Size: 2387 226 152 2109 1697 Fragments arranged by size: 2387 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2393 3077 3572 3896 1870 Size: 73 450 684 495 324 4545 Fragments arranged by size: 4545 684 495 450 324 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 3384 3545 3704 3892 Size: 325 217 627 276 1321 161 159 188 Cuts at: 3892 5347 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1321 627 325 276 217 188 161 159 BanII G_rGCy'C Cuts at: 1391 2531 3084 3376 3720 3890 1391 Size: 1140 553 292 344 170 4072 Fragments arranged by size: 4072 1140 553 344 292 170 BbeI G_GCGC'C Cuts at: 3388 3388 Size: 6571 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2688 2699 928 Size: 971 555 234 11 4800 Fragments arranged by size: 4800 971 555 234 11 BbvCI CC'TCA_GC Cuts at: 3024 3024 Size: 6571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5673 4463 Size: 1210 5361 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 5182 136 Size: 107 122 71 631 605 448 3062 1525 Fragments arranged by size: 3062 1525 631 605 448 122 107 71 BglII A'GATC_T Cuts at: 3060 6566 3060 Size: 3506 3065 BlpI GC'TnA_GC Cuts at: 2039 2907 2984 3988 2039 Size: 868 77 1004 4622 Fragments arranged by size: 4622 1004 868 77 Bme1580I G_kGCm'C Cuts at: 1790 2066 2116 2929 3055 3084 3376 3707 4632 Size: 276 50 813 126 29 292 331 925 Cuts at: 4632 5788 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 925 813 331 292 276 126 50 29 BmgBI CAC'GTC Cuts at: 1318 3580 1318 Size: 2262 4309 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2834 4302 5260 Size: 416 53 83 399 24 1417 1468 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 1753 1468 1417 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2179 3226 2179 Size: 1047 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2708 2951 3077 3813 3814 5231 1239 Size: 869 600 243 126 736 1 1417 2579 Fragments arranged by size: 2579 1417 869 736 600 243 126 1 Bpu10I CC'TnA_GC Cuts at: 3024 3024 Size: 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4696 5561 5859 3643 Size: 1053 865 298 4355 Fragments arranged by size: 4355 1053 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3155 3507 3809 5234 882 Size: 617 1656 352 302 1425 2219 Fragments arranged by size: 2219 1656 1425 617 352 302 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2305 2451 2478 2696 Size: 53 83 186 936 772 146 27 218 Cuts at: 2696 3385 4378 4760 5395 275 Size: 689 993 382 635 1451 Fragments arranged by size: 1451 993 936 772 689 635 382 218 186 146 83 53 27 BsaWI w'CCGG_w Cuts at: 5004 5402 5676 5823 6234 5004 Size: 398 274 147 411 5341 Fragments arranged by size: 5341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 2541 2571 2681 Size: 30 165 30 906 30 202 30 110 Cuts at: 2681 2711 3152 3182 1178 Size: 30 441 30 4567 Fragments arranged by size: 4567 906 441 202 165 110 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2115 2351 2411 2414 2480 2562 3041 Size: 859 70 236 60 3 66 82 479 Cuts at: 3041 3266 1186 Size: 225 4491 Fragments arranged by size: 4491 859 479 236 225 82 70 66 60 3 BseYI C'CCAG_C Cuts at: 2059 2218 2388 2530 2839 3539 3786 5774 6270 Size: 159 170 142 309 700 247 1988 496 Cuts at: 6270 2059 Size: 2360 Fragments arranged by size: 2360 1988 700 496 309 247 170 159 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 2591 1163 Size: 573 597 258 5143 Fragments arranged by size: 5143 597 573 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4632 4717 5788 4632 Size: 85 1071 5415 Fragments arranged by size: 5415 1071 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2116 2531 2929 3055 3084 3376 Size: 399 276 50 415 398 126 29 292 Cuts at: 3376 3707 3720 3890 4632 4717 5788 1391 Size: 331 13 170 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 398 331 292 276 170 126 85 50 29 13 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2421 3193 4460 6190 2421 Size: 772 1267 1730 2802 Fragments arranged by size: 2802 1730 1267 772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 2796 3926 844 Size: 1443 509 1130 3489 Fragments arranged by size: 3489 1443 1130 509 BsrBI CCG'CTC Cuts at: 1394 1823 3256 4458 1394 Size: 429 1433 1202 3507 Fragments arranged by size: 3507 1433 1202 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2144 3381 5215 5402 1700 Size: 110 334 1237 1834 187 2869 Fragments arranged by size: 2869 1834 1237 334 187 110 BsrGI T'GTAC_A Cuts at: 96 3168 96 Size: 3072 3499 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 2287 2362 2413 794 Size: 1493 75 51 4952 Fragments arranged by size: 4952 1493 75 51 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstXI CCAn_nnnn'nTGG Cuts at: 3838 3838 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2430 2583 2934 3060 3177 3901 4268 4653 Size: 458 153 351 126 117 724 367 385 Cuts at: 4653 4670 6122 6208 6220 6566 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 724 458 385 367 351 346 153 126 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2139 3662 2139 Size: 1523 5048 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 2382 2844 3837 513 Size: 552 939 20 358 462 993 3247 Fragments arranged by size: 3247 993 939 552 462 358 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 2377 3505 3619 3679 4087 4881 4901 2377 Size: 1128 114 60 408 794 20 4047 Fragments arranged by size: 4047 1128 794 408 114 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2805 2973 Size: 54 1425 50 275 18 177 798 168 Cuts at: 2973 3099 4018 4907 6536 8 Size: 126 919 889 1629 43 Fragments arranged by size: 1629 1425 919 889 798 275 177 168 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3771 4501 1353 Size: 2418 730 3423 Fragments arranged by size: 3423 2418 730 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2708 2951 3077 3155 3813 3814 Size: 393 476 600 243 126 78 658 1 Cuts at: 3814 4634 5231 6018 6286 1239 Size: 820 597 787 268 1524 Fragments arranged by size: 1524 820 787 658 600 597 476 393 268 243 126 78 1 EcoNI CCTnn'n_nnAGG Cuts at: 2541 3137 2541 Size: 596 5975 EcoO109I rG'GnC_Cy Cuts at: 2947 3372 3373 3584 2947 Size: 425 1 211 5934 Fragments arranged by size: 5934 425 211 1 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 6571 FspI TGC'GCA Cuts at: 3362 5077 3362 Size: 1715 4856 HaeII r_GCGC'y Cuts at: 1678 2176 3280 3291 3388 5718 1678 Size: 498 1104 11 97 2330 2531 Fragments arranged by size: 2531 2330 1104 498 97 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 5212 5244 5286 Size: 32 135 32 1109 32 2499 32 42 Cuts at: 5286 5318 1373 Size: 32 2626 Fragments arranged by size: 2626 2499 1109 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3349 3915 4161 669 Size: 988 281 1411 566 246 3079 Fragments arranged by size: 3079 1411 988 566 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KasI G'GCGC_C Cuts at: 3384 3384 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 5685 5869 1305 Size: 1116 3264 184 2007 Fragments arranged by size: 3264 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2807 2975 3101 10 Size: 54 1425 343 177 798 168 126 3480 Fragments arranged by size: 3480 1425 798 343 177 168 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2015 3239 4529 4888 5047 518 Size: 648 843 6 1224 1290 359 159 2042 Fragments arranged by size: 2042 1290 1224 843 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2026 3312 3593 3633 3987 4664 5812 6057 Size: 420 1286 281 40 354 677 1148 245 Cuts at: 6057 1606 Size: 2120 Fragments arranged by size: 2120 1286 1148 677 420 354 281 245 40 NaeI GCC'GGC Cuts at: 2146 2146 Size: 6571 NarI GG'CG_CC Cuts at: 3385 3385 Size: 6571 NcoI C'CATG_G Cuts at: 513 1065 2004 3837 513 Size: 552 939 1833 3247 Fragments arranged by size: 3247 1833 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NgoMIV G'CCGG_C Cuts at: 2144 2144 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3929 5474 1132 Size: 97 2700 1545 2229 Fragments arranged by size: 2700 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 1108 Size: 978 5593 PfoI T'CCnGG_A Cuts at: 2811 2937 3651 2811 Size: 126 714 5731 Fragments arranged by size: 5731 714 126 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 3584 2947 Size: 637 5934 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 3080 3372 3080 Size: 292 6279 PspXI vC'TCGA_Gb Cuts at: 2393 3572 2393 Size: 1179 5392 PstI C_TGCA'G Cuts at: 830 2367 3699 3923 830 Size: 1537 1332 224 3478 Fragments arranged by size: 3478 1537 1332 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 3312 3593 3312 Size: 281 6290 SacII CC_GC'GG Cuts at: 2027 2027 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SapI GCTCTTCn'nnn_ Cuts at: 3771 3771 Size: 6571 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SexAI A'CCwGG_T Cuts at: 2613 2613 Size: 6571 SfcI C'TryA_G Cuts at: 826 1045 2265 2363 3588 3695 3919 5054 5735 Size: 219 1220 98 1225 107 224 1135 681 Cuts at: 5735 5926 826 Size: 191 1471 Fragments arranged by size: 1471 1225 1220 1135 681 224 219 191 107 98 SfoI GGC'GCC Cuts at: 3386 3386 Size: 6571 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3572 3622 4675 5576 5838 Size: 197 926 450 1179 50 1053 901 262 Cuts at: 5838 820 Size: 1553 Fragments arranged by size: 1553 1179 1053 926 901 450 262 197 50 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 3038 4495 5 Size: 47 2986 1457 2081 Fragments arranged by size: 2986 2081 1457 47 StuI AGG'CCT Cuts at: 1517 3323 1517 Size: 1806 4765 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 3471 3837 4010 513 Size: 552 939 63 1404 366 173 3074 Fragments arranged by size: 3074 1404 939 552 366 173 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2853 3546 4598 4615 4768 4953 5398 Size: 1255 1273 693 1052 17 153 185 445 Cuts at: 5398 325 Size: 1498 Fragments arranged by size: 1498 1273 1255 1052 693 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 2895 3168 Size: 274 80 33 51 1443 792 126 273 Cuts at: 3168 4817 6529 96 Size: 1649 1712 138 Fragments arranged by size: 1712 1649 1443 792 274 273 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 2541 2573 2919 2951 6531 6563 2309 Size: 32 200 32 346 32 3580 32 2317 Fragments arranged by size: 3580 2317 346 200 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2491 3115 2011 Size: 480 624 5467 Fragments arranged by size: 5467 624 480 XhoI C'TCGA_G Cuts at: 1943 2393 3572 1943 Size: 450 1179 4942 Fragments arranged by size: 4942 1179 450 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2479 2697 4379 276 Size: 53 83 186 936 945 218 1682 2468 Fragments arranged by size: 2468 1682 945 936 218 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SfiI SrfI SwaI