(Circular) (Six-base) MAPSORT of: pFN21AB8672.seq Check: 9407 from: 1 to: 6418 pFN21AB8672 6418 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2193 3761 1937 Size: 256 1568 4594 Fragments arranged by size: 4594 1568 256 Acc65I G'GTAC_C Cuts at: 3739 3739 Size: 6418 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3576 4481 5865 6133 1632 Size: 1944 905 1384 268 1917 Fragments arranged by size: 1944 1917 1384 905 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6221 AflIII A'CryG_T Cuts at: 1203 3297 5317 6294 1203 Size: 2094 2020 977 1327 Fragments arranged by size: 2094 2020 1327 977 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6418 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2630 2662 2630 Size: 32 6386 AlwNI CAG_nnn'CTG Cuts at: 2216 3013 3442 4161 5733 2216 Size: 797 429 719 1572 2901 Fragments arranged by size: 2901 1572 797 719 429 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 2078 2434 2752 3727 3953 4105 6214 1493 Size: 585 356 318 975 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 585 356 318 226 152 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 3743 1870 Size: 73 1800 4545 Fragments arranged by size: 4545 1800 73 BamHI G'GATC_C Cuts at: 2048 3748 4115 2048 Size: 1700 367 4351 Fragments arranged by size: 4351 1700 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3739 5194 618 Size: 325 217 627 1952 1455 1842 Fragments arranged by size: 1952 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2163 2877 3737 1391 Size: 772 714 860 4072 Fragments arranged by size: 4072 860 772 714 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 928 Size: 971 606 4841 Fragments arranged by size: 4841 971 606 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2656 4310 5520 2656 Size: 1654 1210 3554 Fragments arranged by size: 3554 1654 1210 BclI T'GATC_A Cuts at: 1548 2409 1548 Size: 861 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 5029 136 Size: 107 122 71 631 605 901 2456 1525 Fragments arranged by size: 2456 1525 901 631 605 122 107 71 BglII A'GATC_T Cuts at: 6413 6413 Size: 6418 BlpI GC'TnA_GC Cuts at: 2871 3835 2871 Size: 964 5454 Bme1580I G_kGCm'C Cuts at: 1790 3357 4479 5635 1790 Size: 1567 1122 1156 2573 Fragments arranged by size: 2573 1567 1156 1122 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2257 2600 4149 Size: 416 53 83 399 24 840 343 1549 Cuts at: 4149 5107 442 Size: 958 1753 Fragments arranged by size: 1753 1549 958 840 416 399 343 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 5078 1239 Size: 1565 2274 2579 Fragments arranged by size: 2579 2274 1565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4543 5408 5706 4543 Size: 865 298 5255 Fragments arranged by size: 5255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2908 5081 882 Size: 617 1409 2173 2219 Fragments arranged by size: 2219 2173 1409 617 BsaAI yAC'GTr Cuts at: 493 1204 4209 493 Size: 711 3005 2702 Fragments arranged by size: 3005 2702 711 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3387 4225 4607 5242 Size: 53 83 186 936 1854 838 382 635 Cuts at: 5242 275 Size: 1451 Fragments arranged by size: 1854 1451 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4851 5249 5523 5670 6081 4851 Size: 398 274 147 411 5188 Fragments arranged by size: 5188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6193 Fragments arranged by size: 6193 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6418 BseYI C'CCAG_C Cuts at: 2695 5621 6117 2695 Size: 2926 496 2996 Fragments arranged by size: 2996 2926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5845 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 4479 4564 5635 2163 Size: 2316 85 1071 2946 Fragments arranged by size: 2946 2316 1071 85 BsmI GAATG_Cn' Cuts at: 2266 2530 3929 4022 2266 Size: 264 1399 93 4662 Fragments arranged by size: 4662 1399 264 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2877 3357 3737 4479 4564 5635 Size: 399 373 714 480 380 742 85 1071 Cuts at: 5635 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 714 480 399 380 373 85 BspEI T'CCGG_A Cuts at: 6081 6081 Size: 6418 BspHI T'CATG_A Cuts at: 4307 6037 4307 Size: 1730 4688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3773 844 Size: 2929 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 3606 4913 5087 65 Size: 1183 2142 216 1307 174 1396 Fragments arranged by size: 2142 1396 1307 1183 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5062 5249 1700 Size: 110 3252 187 2869 Fragments arranged by size: 3252 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3487 96 Size: 3391 3027 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 4478 5490 4478 Size: 1012 5406 BstAPI GCAn_nnn'nTGC Cuts at: 794 2429 794 Size: 1635 4783 BstBI TT'CG_AA Cuts at: 5222 5222 Size: 6418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2048 3748 4115 4500 4517 5969 6055 6067 Size: 76 1700 367 385 17 1452 86 12 Cuts at: 6067 6413 1972 Size: 346 1977 Fragments arranged by size: 1977 1700 1452 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2377 2571 513 Size: 552 1312 194 4360 Fragments arranged by size: 4360 1312 552 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5679 Fragments arranged by size: 5679 683 56 BtsI GCAGTG_nn' Cuts at: 3934 4728 4748 3934 Size: 794 20 5604 Fragments arranged by size: 5604 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2439 2581 Size: 54 1425 50 275 18 225 384 142 Cuts at: 2581 3865 4754 6383 8 Size: 1284 889 1629 43 Fragments arranged by size: 1629 1425 1284 889 384 275 225 142 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 4348 1353 Size: 1368 1627 3423 Fragments arranged by size: 3423 1627 1368 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2161 2161 Size: 6418 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2804 3576 4481 5078 5865 6133 1239 Size: 393 1172 772 905 597 787 268 1524 Fragments arranged by size: 1524 1172 905 787 772 597 393 268 EcoRI G'AATT_C Cuts at: 3727 4105 3727 Size: 378 6040 EcoRV GAT'ATC Cuts at: 3226 3226 Size: 6418 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2735 2735 Size: 6418 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 5565 1678 Size: 3887 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2223 2255 2373 2405 3215 Size: 32 135 32 651 32 118 32 810 Cuts at: 3215 3247 5059 5091 5133 5165 1373 Size: 32 1812 32 42 32 2626 Fragments arranged by size: 2626 1812 810 651 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 3762 4008 669 Size: 988 281 615 1209 246 3079 Fragments arranged by size: 3079 1209 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 3558 748 Size: 2810 3608 HpaI GTT'AAC Cuts at: 2553 4008 2553 Size: 1455 4963 KpnI G_GTAC'C Cuts at: 3743 3743 Size: 6418 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5532 5716 1305 Size: 4227 184 2007 Fragments arranged by size: 4227 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 2583 10 Size: 54 1425 343 609 142 3845 Fragments arranged by size: 3845 1425 609 343 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2355 2654 4376 4735 4894 518 Size: 648 1167 22 299 1722 359 159 2042 Fragments arranged by size: 2042 1722 1167 648 359 299 159 22 MspA1I CmG'CkG Cuts at: 1606 2749 3834 4511 5659 5904 1606 Size: 1143 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1143 1085 677 245 NcoI C'CATG_G Cuts at: 513 1065 2377 2571 513 Size: 552 1312 194 4360 Fragments arranged by size: 4360 1312 552 194 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 3301 3776 5321 1132 Size: 97 2072 475 1545 2229 Fragments arranged by size: 2229 2072 1545 475 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6418 PciI A'CATG_T Cuts at: 3297 5317 3297 Size: 2020 4398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6418 PfoI T'CCnGG_A Cuts at: 3330 3330 Size: 6418 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4487 4519 6000 6032 4487 Size: 32 1481 32 4873 Fragments arranged by size: 4873 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2189 1613 Size: 576 5842 PsiI TTA'TAA Cuts at: 3479 3988 3479 Size: 509 5909 PstI C_TGCA'G Cuts at: 830 3770 830 Size: 2940 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 PvuII CAG'CTG Cuts at: 2749 2749 Size: 6418 SacI G_AGCT'C Cuts at: 2163 2163 Size: 6418 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 2446 4666 6378 2446 Size: 2220 1712 2486 Fragments arranged by size: 2486 2220 1712 SfcI C'TryA_G Cuts at: 826 1045 3075 3766 4901 5582 5773 826 Size: 219 2030 691 1135 681 191 1471 Fragments arranged by size: 2030 1471 1135 691 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4522 5423 5685 820 Size: 197 926 2579 901 262 1553 Fragments arranged by size: 2579 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 2428 3325 4342 5 Size: 47 2376 897 1017 2081 Fragments arranged by size: 2376 2081 1017 897 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6418 StyI C'CwwG_G Cuts at: 513 1065 2052 2092 2377 2571 3857 513 Size: 552 987 40 285 194 1286 3074 Fragments arranged by size: 3074 1286 987 552 285 194 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 2792 3395 4445 4462 4615 4800 Size: 1255 659 553 603 1050 17 153 185 Cuts at: 4800 5245 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1050 659 603 553 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2444 3487 4664 Size: 274 80 33 51 1443 467 1043 1177 Cuts at: 4664 6376 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1177 1043 467 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6378 6410 6378 Size: 32 6386 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 4547 4547 Size: 6418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI