(Circular) (Six-base) MAPSORT of: pFN21AB8671.seq Check: 915 from: 1 to: 5893 pFN21AB8671 5893 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2682 3236 1937 Size: 745 554 4594 Fragments arranged by size: 4594 745 554 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2130 2444 3060 3956 5340 5608 1632 Size: 498 314 616 896 1384 268 1917 Fragments arranged by size: 1917 1384 896 616 498 314 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 2375 4792 5769 1203 Size: 1172 2417 977 1327 Fragments arranged by size: 2417 1327 1172 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 2320 3636 5208 2320 Size: 1316 1572 3005 Fragments arranged by size: 3005 1572 1316 ApaI G_GGCC'C Cuts at: 2490 2490 Size: 5893 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2253 3161 3202 3428 3580 5689 1493 Size: 760 908 41 226 152 2109 1697 Fragments arranged by size: 2109 1697 908 760 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3218 1870 Size: 73 1275 4545 Fragments arranged by size: 4545 1275 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2831 2864 2831 Size: 33 5860 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3214 4669 618 Size: 325 217 627 1427 1455 1842 Fragments arranged by size: 1842 1455 1427 627 325 217 BanII G_rGCy'C Cuts at: 1391 2305 2490 3212 1391 Size: 914 185 722 4072 Fragments arranged by size: 4072 914 722 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4922 BbvCI CC'TCA_GC Cuts at: 2832 2832 Size: 5893 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4084 4118 1903 Size: 34 50 34 2063 34 3678 Fragments arranged by size: 3678 2063 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 2490 3954 5110 1790 Size: 700 1464 1156 2573 Fragments arranged by size: 2573 1464 1156 700 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4553 1239 Size: 3314 2579 Bpu10I CC'TnA_GC Cuts at: 2832 2832 Size: 5893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2795 4018 4883 5181 2795 Size: 1223 865 298 3507 Fragments arranged by size: 3507 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2378 3684 493 Size: 711 1174 1306 2702 Fragments arranged by size: 2702 1306 1174 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 4082 4717 275 Size: 53 83 186 936 2167 382 635 1451 Fragments arranged by size: 2167 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4326 4724 4998 5145 5556 4326 Size: 398 274 147 411 4663 Fragments arranged by size: 4663 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2968 2998 1178 Size: 30 165 30 1565 30 4073 Fragments arranged by size: 4073 1565 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2538 2541 2849 2976 1186 Size: 1352 3 308 127 4103 Fragments arranged by size: 4103 1352 308 127 3 BseYI C'CCAG_C Cuts at: 2897 5096 5592 2897 Size: 2199 496 3198 Fragments arranged by size: 3198 2199 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2152 1163 Size: 573 416 4904 Fragments arranged by size: 4904 573 416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2305 3954 4039 5110 2305 Size: 1649 85 1071 3088 Fragments arranged by size: 3088 1649 1071 85 BsmI GAATG_Cn' Cuts at: 2332 3404 3497 2332 Size: 1072 93 4728 Fragments arranged by size: 4728 1072 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2305 2490 3212 3954 4039 5110 1391 Size: 399 515 185 722 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 722 515 399 185 85 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 2614 3782 5512 2614 Size: 1168 1730 2995 Fragments arranged by size: 2995 1730 1168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4388 4562 65 Size: 1183 3140 174 1396 Fragments arranged by size: 3140 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2422 3223 3590 3975 3992 5444 5530 5542 Size: 450 801 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 801 450 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2988 2988 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 2931 3409 4203 4223 2931 Size: 478 794 20 4601 Fragments arranged by size: 4601 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3064 3197 3064 Size: 133 5760 DraIII CAC_nnn'GTG Cuts at: 2873 2873 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858 Size: 54 1425 50 275 18 1510 889 1629 Cuts at: 5858 8 Size: 43 Fragments arranged by size: 1629 1510 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2448 3823 1353 Size: 1095 1375 3423 Fragments arranged by size: 3423 1375 1095 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2303 2303 Size: 5893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2444 3060 3956 4553 5340 5608 Size: 393 498 314 616 896 597 787 268 Cuts at: 5608 1239 Size: 1524 Fragments arranged by size: 1524 896 787 616 597 498 393 314 268 EcoO109I rG'GnC_Cy Cuts at: 2317 2799 2317 Size: 482 5411 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 EcoRV GAT'ATC Cuts at: 2398 2398 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2968 3000 3135 3167 4534 Size: 32 135 32 1396 32 135 32 1367 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1396 1367 135 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2130 3237 3483 669 Size: 988 281 192 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 192 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 2130 3483 2130 Size: 1353 4540 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2068 2938 5007 5191 1305 Size: 763 870 2069 184 2007 Fragments arranged by size: 2069 2007 870 763 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2346 3851 4210 4369 518 Size: 648 1180 1505 359 159 2042 Fragments arranged by size: 2042 1505 1180 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3309 3986 5134 5379 1606 Size: 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3251 4796 1132 Size: 97 2022 1545 2229 Fragments arranged by size: 2229 2022 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2051 2102 1108 Size: 943 51 4899 Fragments arranged by size: 4899 943 51 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PmlI CAC'GTG Cuts at: 2378 2378 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PspOMI G'GGCC_C Cuts at: 2486 2486 Size: 5893 PstI C_TGCA'G Cuts at: 830 2938 2998 3245 830 Size: 2108 60 247 3478 Fragments arranged by size: 3478 2108 247 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacI G_AGCT'C Cuts at: 2305 2305 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SapI GCTCTTCn'nnn_ Cuts at: 2448 2448 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2934 2994 3241 4376 5057 5248 826 Size: 219 1889 60 247 1135 681 191 1471 Fragments arranged by size: 1889 1471 1135 681 247 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 3997 4898 5160 820 Size: 197 926 867 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2802 3332 513 Size: 552 1737 530 3074 Fragments arranged by size: 3074 1737 552 530 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2743 3920 3937 4090 4275 4720 325 Size: 1255 1163 1177 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1177 1163 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3105 4139 5851 Size: 274 80 33 51 1443 1128 1034 1712 Cuts at: 5851 96 Size: 138 Fragments arranged by size: 1712 1443 1128 1034 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 5853 5885 2161 Size: 32 3660 32 2169 Fragments arranged by size: 3660 2169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 2457 4022 2457 Size: 1565 4328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI