Restriction map for
pFN21AB8670
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BseRI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
EcoNI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AclI 850 AACGTT AA^CGTT
AcyI 265 GGCGCC G[GA]^CG[CT]C
AflIII 1065 ACATGT A^C[GA][CT]GT
AocI 511 CCTCAGG CC^T[ACGT]AGG
AspHI 548 GAGCTC G[AT]GC[AT]^C
BamHI 284 GGATCC G^GATCC
BbeI 265 GGCGCC GGCGC^C
BcgI 156 CGAGCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1111 CTAG C^TAG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BsaAI 148 TACGTG [CT]AC^GT[GA]
BsaHI 265 GGCGCC G[GA]^CG[CT]C
BsiHKAI 548 GAGCTC G[AT]GC[AT]^C
BsmBI 489 CGTCTC CGTCTC(1/5)
BspLU11I 1065 ACATGT A^CATGT
BsrBI 48 GAGCGG CCGCTC(-3/-3)
BsrDI 612 GCAATG GCAATG(2/0)
BssHII 159 GCGCGC G^CGCGC
BstEII 1368 GGTGACC G^GT[ACGT]ACC
BstPI 1368 GGTGACC G^GT[ACGT]ACC
BstXI 818 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 284 GGATCC [GA]^GATC[CT]
Bsu36I 511 CCTCAGG CC^T[ACGT]AGG
CelII 45 GCTGAGC GC^T[ACGT]AGC
DraII 253 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1363 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 543 CGGCCG C^GGCCG
EclXI 543 CGGCCG C^GGCCG
Eco52I 543 CGGCCG C^GGCCG
Eco81I 511 CCTCAGG CC^T[ACGT]AGG
EcoO109I 253 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1368 GGTGACC G^GT[ACGT]ACC
EcoRI 1088 GAATTC G^AATTC
EcoT14I 1309 CCATGG C^C[AT][AT]GG
HgaI 179 GCGTC GACGC(5/10)
Hin1I 265 GGCGCC G[GA]^CG[CT]C
HincII 564 GTTGAC GT[CT]^[GA]AC
HindII 564 GTTGAC GT[CT]^[GA]AC
KasI 265 GGCGCC G^GCGCC
MaeI 1111 CTAG C^TAG
MflI 284 GGATCC [GA]^GATC[CT]
MseI 1051 TTAA T^TAA
MslI 963 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 265 GGCGCC GG^CGCC
NcoI 1309 CCATGG C^CATGG
PflMI 144 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 850 AACGTT AA^CGTT
PvuI 174 CGATCG CGAT^CG
PvuII 940 CAGCTG CAG^CTG
SacI 548 GAGCTC GAGCT^C
SfoI 265 GGCGCC GGC^GCC
SgrAI 261 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 250 CCCGGG CCC^GGG
SphI 1024 GCATGC GCATG^C
StyI 1309 CCATGG C^C[AT][AT]GG
Tru9I 1051 TTAA T^TAA
Van91I 144 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 699 CCAGGTCGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 284 GGATCC [GA]^GATC[CT]
XmaCI 250 CCCGGG C^CCGGG
XmaI 250 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 641 AAATTT [GA]^AATT[CT]
1088 GAATTC
AfaI 147 GTAC GT^AC
933 GTAC
ApaI 247 GGGCCC GGGCC^C
253 GGGCCC
ApoI 641 AAATTT [GA]^AATT[CT]
1088 GAATTC
Asp700I 596 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAAATGATTC
AvaI 204 CTCGGG C^[CT]CG[GA]G
250 CCCGGG
BalI 687 TGGCCA TGG^CCA
1376 TGGCCA
BbsI 181 GTCTTC GAAGAC(2/6)
583 GTCTTC
BpmI 307 CTGGAG CTGGAG(16/14)
1291 CTGGAG
BpuAI 181 GTCTTC GAAGAC(2/6)
583 GTCTTC
BsaI 833 GAGACC GGTCTC(1/5)
534 GGTCTC
BsgI 1220 CTGCAC GTGCAG(16/14)
1043 GTGCAG
BsmFI 402 GGGAC GGGAC(10/14)
1126 GTCCC
BsoBI 204 CTCGGG C^[CT]CG[GA]G
250 CCCGGG
BspMI 935 ACCTGC ACCTGC(4/8)
531 GCAGGT
BsrFI 101 GCCGGC [GA]^CCGG[CT]
262 GCCGGC
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
262 GCCGGC
MluNI 687 TGGCCA TGG^CCA
1376 TGGCCA
MscI 687 TGGCCA TGG^CCA
1376 TGGCCA
MspA1I 940 CAGCTG C[AC]G^C[GT]G
981 CCGCTG
NaeI 101 GCCGGC GCC^GGC
262 GCCGGC
NgoMIV 101 GCCGGC G^CCGGC
262 GCCGGC
PstI 529 CTGCAG CTGCA^G
937 CTGCAG
RsaI 147 GTAC GT^AC
933 GTAC
SfcI 529 CTGCAG C^T[GA][CT]AG
937 CTGCAG
TaqI 632 TCGA T^CGA
683 TCGA
TthHB8I 632 TCGA T^CGA
683 TCGA
XmnI 596 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiEI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp45I Tsp509I TspRI