(Circular) (Six-base) MAPSORT of: pFN21AB8670.seq Check: 6199 from: 1 to: 6097 pFN21AB8670 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 2856 4224 4597 1563 Size: 1293 1368 373 3063 Fragments arranged by size: 3063 1368 1293 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2185 2575 4160 5544 5812 1632 Size: 553 390 1585 1384 268 1917 Fragments arranged by size: 1917 1585 1384 553 390 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 3070 4996 5973 1203 Size: 1867 1926 977 1327 Fragments arranged by size: 1926 1867 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 2972 1166 Size: 1806 4291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaI G_GGCC'C Cuts at: 2256 2262 2256 Size: 6 6091 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2646 3093 3406 3632 3784 5893 1493 Size: 1153 447 313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1153 447 313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2209 2255 3422 1870 Size: 73 266 46 1167 4545 Fragments arranged by size: 4545 1167 266 73 46 BamHI G'GATC_C Cuts at: 2289 3427 3794 2289 Size: 1138 367 4592 Fragments arranged by size: 4592 1138 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2239 2270 3276 3418 Size: 325 217 627 350 102 31 1006 142 Cuts at: 3418 4873 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1006 627 350 325 217 142 102 31 BanII G_rGCy'C Cuts at: 1391 2035 2256 2262 2487 2557 3416 1391 Size: 644 221 6 225 70 859 4072 Fragments arranged by size: 4072 859 644 225 221 70 6 BbeI G_GCGC'C Cuts at: 2274 2274 Size: 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2179 2581 928 Size: 971 280 402 4444 Fragments arranged by size: 4444 971 402 280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2150 2184 4288 4322 1903 Size: 34 213 34 2104 34 3678 Fragments arranged by size: 3678 2104 213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 2051 3514 2051 Size: 1463 4634 Bme1580I G_kGCm'C Cuts at: 1790 2079 2142 2244 2256 2262 2304 2985 4158 Size: 289 63 102 12 6 42 681 1173 Cuts at: 4158 5314 1790 Size: 1156 2573 Fragments arranged by size: 2573 1173 1156 681 289 102 63 42 12 6 BmgBI CAC'GTC Cuts at: 1318 3349 1318 Size: 2031 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 3317 4757 1239 Size: 1094 984 1440 2579 Fragments arranged by size: 2579 1440 1094 984 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2545 2832 4760 882 Size: 617 1046 287 1928 2219 Fragments arranged by size: 2219 1928 1046 617 287 BsaAI yAC'GTr Cuts at: 493 1204 2155 3888 493 Size: 711 951 1733 2702 Fragments arranged by size: 2702 1733 951 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 3904 4286 4921 Size: 53 83 186 936 738 1633 382 635 Cuts at: 4921 275 Size: 1451 Fragments arranged by size: 1633 1451 936 738 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2841 2871 3290 3320 1178 Size: 30 165 30 1438 30 419 30 3955 Fragments arranged by size: 3955 1438 419 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6097 BseYI C'CCAG_C Cuts at: 2027 2038 2065 2218 2243 2830 5300 5796 2027 Size: 11 27 153 25 587 2470 496 2328 Fragments arranged by size: 2470 2328 587 496 153 27 25 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3069 3210 1163 Size: 573 1333 141 4050 Fragments arranged by size: 4050 1333 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2182 2279 2551 3292 3547 4308 Size: 275 186 181 97 272 741 255 761 Cuts at: 4308 5336 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 741 275 272 255 186 181 97 BsiHKAI G_wGCw'C Cuts at: 2557 4158 4243 5314 2557 Size: 1601 85 1071 3340 Fragments arranged by size: 3340 1601 1071 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 BsmBI CGTCTCn'nnnn_ Cuts at: 2500 2500 Size: 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2079 2142 2244 2256 2262 2304 Size: 399 245 44 63 102 12 6 42 Cuts at: 2304 2487 2557 2985 3416 4158 4243 5314 1391 Size: 183 70 428 431 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 428 399 245 183 102 85 70 63 44 42 12 6 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2527 2949 3452 844 Size: 1683 422 503 3489 Fragments arranged by size: 3489 1683 503 422 BsrBI CCG'CTC Cuts at: 1394 1823 2055 3984 1394 Size: 429 232 1929 3507 Fragments arranged by size: 3507 1929 429 232 BsrDI GCAATG_nn' Cuts at: 65 1248 2624 4592 4766 65 Size: 1183 1376 1968 174 1396 Fragments arranged by size: 1968 1396 1376 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 2267 4741 4928 1700 Size: 110 296 161 2474 187 2869 Fragments arranged by size: 2869 2474 296 187 161 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 2164 1925 Size: 239 5858 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 3373 1184 Size: 2189 3908 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2830 2011 Size: 819 5278 BstYI r'GATC_y Cuts at: 1972 2289 3427 3794 4179 4196 5648 5734 5746 Size: 317 1138 367 385 17 1452 86 12 Cuts at: 5746 6092 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 317 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2517 2517 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2671 3314 513 Size: 552 939 138 529 643 3296 Fragments arranged by size: 3296 939 643 552 529 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2360 3057 3613 4407 4427 2360 Size: 697 556 794 20 4030 Fragments arranged by size: 4030 794 697 556 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2815 2850 3099 3134 3139 3174 529 Size: 35 2251 35 249 35 5 35 3452 Fragments arranged by size: 3452 2251 249 35 35 35 35 5 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DraIII CAC_nnn'GTG Cuts at: 3373 3373 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2548 2692 Size: 54 1425 50 275 18 258 460 144 Cuts at: 2692 2892 3381 3544 4433 6062 8 Size: 200 489 163 889 1629 43 Fragments arranged by size: 1629 1425 889 489 460 275 258 200 163 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2548 3544 1537 Size: 275 736 996 4090 Fragments arranged by size: 4090 996 736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 1353 Size: 2674 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2147 2890 4684 5058 5204 387 Size: 1064 138 558 743 1794 374 146 1280 Fragments arranged by size: 1794 1280 1064 743 558 374 146 138 EcoICRI GAG'CTC Cuts at: 2555 2555 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2333 2575 3317 4160 4757 5544 Size: 393 553 148 242 742 843 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 843 787 742 597 553 393 268 242 148 EcoO109I rG'GnC_Cy Cuts at: 2259 2259 Size: 6097 EcoRI G'AATT_C Cuts at: 3093 3406 3784 3093 Size: 313 378 5406 Fragments arranged by size: 5406 378 313 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2012 2098 2219 2274 5244 1678 Size: 334 86 121 55 2970 2531 Fragments arranged by size: 2970 2531 334 121 86 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3195 3227 4738 4770 4812 Size: 32 135 32 1623 32 1511 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1623 1511 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2571 3441 3687 669 Size: 988 281 633 870 246 3079 Fragments arranged by size: 3079 988 870 633 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2270 2270 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 5211 5395 1305 Size: 1155 2751 184 2007 Fragments arranged by size: 2751 2007 1155 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2694 3383 10 Size: 54 1425 343 862 689 2724 Fragments arranged by size: 2724 1425 862 689 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2972 4055 4414 4573 518 Size: 648 1806 1083 359 159 2042 Fragments arranged by size: 2042 1806 1083 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2947 2988 3513 4190 5338 5583 1606 Size: 1341 41 525 677 1148 245 2120 Fragments arranged by size: 2120 1341 1148 677 525 245 41 NaeI GCC'GGC Cuts at: 2108 2269 2108 Size: 161 5936 NarI GG'CG_CC Cuts at: 2271 2271 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 2004 3314 513 Size: 552 939 1310 3296 Fragments arranged by size: 3296 1310 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NgoMIV G'CCGG_C Cuts at: 2106 2267 2106 Size: 161 5936 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3033 3074 3357 3455 5000 1132 Size: 97 1804 41 283 98 1545 2229 Fragments arranged by size: 2229 1804 1545 283 98 97 41 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 3070 4996 3070 Size: 1926 4171 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 1108 Size: 1047 5050 PfoI T'CCnGG_A Cuts at: 2099 2099 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2252 2258 2252 Size: 6 6091 PstI C_TGCA'G Cuts at: 830 2538 2946 3449 830 Size: 1708 408 503 3478 Fragments arranged by size: 3478 1708 503 408 PvuI CG_AT'CG Cuts at: 2001 2182 2001 Size: 181 5916 PvuII CAG'CTG Cuts at: 2947 2947 Size: 6097 SacI G_AGCT'C Cuts at: 2557 2557 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2534 2942 3445 4580 5261 5452 826 Size: 219 1489 408 503 1135 681 191 1471 Fragments arranged by size: 1489 1471 1135 681 503 408 219 191 SfoI GGC'GCC Cuts at: 2272 2272 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 2267 1700 Size: 567 5530 SmaI CCC'GGG Cuts at: 1872 2257 3424 1872 Size: 385 1167 4545 Fragments arranged by size: 4545 1167 385 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3033 3455 1132 Size: 1901 422 3774 Fragments arranged by size: 3774 1901 422 SrfI GCCC'GGGC Cuts at: 2257 2257 Size: 6097 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 3314 3536 513 Size: 552 939 1310 222 3074 Fragments arranged by size: 3074 1310 939 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2822 3163 3278 4124 4141 4294 4479 Size: 1255 1242 341 115 846 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1242 846 445 341 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 6057 6089 2616 Size: 32 3409 32 2624 Fragments arranged by size: 3409 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2711 2011 Size: 700 5397 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 2255 3422 1870 Size: 385 1167 4545 Fragments arranged by size: 4545 1167 385 XmnI GAAnn'nnTTC Cuts at: 2605 3141 4226 2605 Size: 536 1085 4476 Fragments arranged by size: 4476 1085 536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI