Restriction map for
pFN21AB8670
(Linear) (Six-base) MAPSORT of: KIBB8670.seq Check: 7790 from: 1 to: 1392
KIBB8670.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AclI AA'CG_TT
Cuts at: 0 851 1392
Size: 851 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 570 1392
Size: 180 390 822
Fragments arranged by size:
822 390 180
AflIII A'CryG_T
Cuts at: 0 1065 1392
Size: 1065 327
AleI CACnn'nnGTG
Cuts at: 0 967 1392
Size: 967 425
ApaI G_GGCC'C
Cuts at: 0 251 257 1392
Size: 251 6 1135
Fragments arranged by size:
1135 251 6
ApoI r'AATT_y
Cuts at: 0 641 1088 1392
Size: 641 447 304
Fragments arranged by size:
641 447 304
AvaI C'yCGr_G
Cuts at: 0 204 250 1392
Size: 204 46 1142
Fragments arranged by size:
1142 204 46
BamHI G'GATC_C
Cuts at: 0 284 1392
Size: 284 1108
BanI G'GyrC_C
Cuts at: 0 132 234 265 1271 1392
Size: 132 102 31 1006 121
Fragments arranged by size:
1006 132 121 102 31
BanII G_rGCy'C
Cuts at: 0 30 251 257 482 552 1392
Size: 30 221 6 225 70 840
Fragments arranged by size:
840 225 221 70 30 6
BbeI G_GCGC'C
Cuts at: 0 269 1392
Size: 269 1123
BbsI GAAGACnn'nnnn_
Cuts at: 0 174 576 1392
Size: 174 402 816
Fragments arranged by size:
816 402 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 179 1392
Size: 145 34 1213
Fragments arranged by size:
1213 145 34
BlpI GC'TnA_GC
Cuts at: 0 46 1392
Size: 46 1346
Bme1580I G_kGCm'C
Cuts at: 0 74 137 239 251 257 299 980 1392
Size: 74 63 102 12 6 42 681 412
Fragments arranged by size:
681 412 102 74 63 42 12 6
BmgBI CAC'GTC
Cuts at: 0 1344 1392
Size: 1344 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1312 1392
Size: 328 984 80
Fragments arranged by size:
984 328 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 540 827 1392
Size: 540 287 565
Fragments arranged by size:
565 540 287
BsaAI yAC'GTr
Cuts at: 0 150 1392
Size: 150 1242
BsaHI Gr'CG_yC
Cuts at: 0 266 1392
Size: 266 1126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 836 866 1285 1315 1392
Size: 836 30 419 30 77
Fragments arranged by size:
836 419 77 30 30
BseYI C'CCAG_C
Cuts at: 0 22 33 60 213 238 825 1392
Size: 22 11 27 153 25 587 567
Fragments arranged by size:
587 567 153 27 25 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1205 1392
Size: 1064 141 187
Fragments arranged by size:
1064 187 141
BsiEI CG_ry'CG
Cuts at: 0 177 274 546 1287 1392
Size: 177 97 272 741 105
Fragments arranged by size:
741 272 177 105 97
BsiHKAI G_wGCw'C
Cuts at: 0 552 1392
Size: 552 840
BsmBI CGTCTCn'nnnn_
Cuts at: 0 495 1392
Size: 495 897
Bsp1286I G_dGCh'C
Cuts at: 0 30 74 137 239 251 257 299 482
Size: 30 44 63 102 12 6 42 183
Cuts at: 482 552 980 1392
Size: 70 428 412
Fragments arranged by size:
428 412 183 102 70 63 44 42
30 12 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 522 944 1392
Size: 522 422 448
Fragments arranged by size:
522 448 422
BsrBI CCG'CTC
Cuts at: 0 50 1392
Size: 50 1342
BsrDI GCAATG_nn'
Cuts at: 0 619 1392
Size: 619 773
BsrFI r'CCGG_y
Cuts at: 0 101 262 1392
Size: 101 161 1130
Fragments arranged by size:
1130 161 101
BssHII G'CGCG_C
Cuts at: 0 159 1392
Size: 159 1233
BstEII G'GTnAC_C
Cuts at: 0 1368 1392
Size: 1368 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1392
Size: 825 567
BstYI r'GATC_y
Cuts at: 0 284 1392
Size: 284 1108
Bsu36I CC'TnA_GG
Cuts at: 0 512 1392
Size: 512 880
BtgI C'CryG_G
Cuts at: 0 137 666 1309 1392
Size: 137 529 643 83
Fragments arranged by size:
643 529 137 83
BtsI GCAGTG_nn'
Cuts at: 0 355 1052 1392
Size: 355 697 340
Fragments arranged by size:
697 355 340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 810 845 1094 1129 1134 1169 1392
Size: 810 35 249 35 5 35 223
Fragments arranged by size:
810 249 223 35 35 35 5
DraIII CAC_nnn'GTG
Cuts at: 0 1368 1392
Size: 1368 24
EaeI y'GGCC_r
Cuts at: 0 83 543 687 887 1376 1392
Size: 83 460 144 200 489 16
Fragments arranged by size:
489 460 200 144 83 16
EagI C'GGCC_G
Cuts at: 0 543 1392
Size: 543 849
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 885 1392
Size: 142 743 507
Fragments arranged by size:
743 507 142
EcoICRI GAG'CTC
Cuts at: 0 550 1392
Size: 550 842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 328 570 1312 1392
Size: 180 148 242 742 80
Fragments arranged by size:
742 242 180 148 80
EcoO109I rG'GnC_Cy
Cuts at: 0 254 1392
Size: 254 1138
EcoRI G'AATT_C
Cuts at: 0 1088 1392
Size: 1088 304
HaeII r_GCGC'y
Cuts at: 0 7 93 214 269 1392
Size: 7 86 121 55 1123
Fragments arranged by size:
1123 121 86 55 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1190 1222 1392
Size: 1190 32 170
Fragments arranged by size:
1190 170 32
HincII GTy'rAC
Cuts at: 0 566 1392
Size: 566 826
KasI G'GCGC_C
Cuts at: 0 265 1392
Size: 265 1127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1392
Size: 455 937
MscI TGG'CCA
Cuts at: 0 689 1378 1392
Size: 689 689 14
Fragments arranged by size:
689 689 14
MslI CAynn'nnrTG
Cuts at: 0 967 1392
Size: 967 425
MspA1I CmG'CkG
Cuts at: 0 942 983 1392
Size: 942 41 409
Fragments arranged by size:
942 409 41
NaeI GCC'GGC
Cuts at: 0 103 264 1392
Size: 103 161 1128
Fragments arranged by size:
1128 161 103
NarI GG'CG_CC
Cuts at: 0 266 1392
Size: 266 1126
NcoI C'CATG_G
Cuts at: 0 1309 1392
Size: 1309 83
NgoMIV G'CCGG_C
Cuts at: 0 101 262 1392
Size: 101 161 1130
Fragments arranged by size:
1130 161 101
NspI r_CATG'y
Cuts at: 0 1028 1069 1352 1392
Size: 1028 41 283 40
Fragments arranged by size:
1028 283 41 40
PciI A'CATG_T
Cuts at: 0 1065 1392
Size: 1065 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 1392
Size: 150 1242
PfoI T'CCnGG_A
Cuts at: 0 94 1392
Size: 94 1298
PspOMI G'GGCC_C
Cuts at: 0 247 253 1392
Size: 247 6 1139
Fragments arranged by size:
1139 247 6
PstI C_TGCA'G
Cuts at: 0 533 941 1392
Size: 533 408 451
Fragments arranged by size:
533 451 408
PvuI CG_AT'CG
Cuts at: 0 177 1392
Size: 177 1215
PvuII CAG'CTG
Cuts at: 0 942 1392
Size: 942 450
SacI G_AGCT'C
Cuts at: 0 552 1392
Size: 552 840
SfcI C'TryA_G
Cuts at: 0 529 937 1392
Size: 529 408 455
Fragments arranged by size:
529 455 408
SfoI GGC'GCC
Cuts at: 0 267 1392
Size: 267 1125
SgrAI Cr'CCGG_yG
Cuts at: 0 262 1392
Size: 262 1130
SmaI CCC'GGG
Cuts at: 0 252 1392
Size: 252 1140
SphI G_CATG'C
Cuts at: 0 1028 1392
Size: 1028 364
SrfI GCCC'GGGC
Cuts at: 0 252 1392
Size: 252 1140
StyI C'CwwG_G
Cuts at: 0 1309 1392
Size: 1309 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 1158 1273 1392
Size: 817 341 115 119
Fragments arranged by size:
817 341 119 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 1392
Size: 611 32 749
Fragments arranged by size:
749 611 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 706 1392
Size: 706 686
XmaI C'CCGG_G
Cuts at: 0 250 1392
Size: 250 1142
XmnI GAAnn'nnTTC
Cuts at: 0 600 1136 1392
Size: 600 536 256
Fragments arranged by size:
600 536 256
Enzymes that do cut:
AclI AcuI AflIII AleI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BlpI Bme1580I BmgBI
BpmI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PspOMI
PstI PvuI PvuII SacI SfcI SfoI SgrAI SmaI
SphI SrfI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI BclI BfrBI BglI BglII BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BseRI BsiWI
BsmI BspEI BspHI BsrGI BssSI BstAPI BstBI BstZ17I
BtgZI ClaI DraI DrdI EarI EcoNI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SspI StuI SwaI TatI
Tth111I XbaI XhoI ZraI