Restriction map for
pFN21AB8667
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstF5I BstPI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 436 GTATAC GT^[AC][GT]AC
AfaI 38 GTAC GT^AC
AlwNI 178 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1224 GGGCCC GGGCC^C
Asp700I 1125 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 869 GTCTTC GAAGAC(2/6)
BcgI 1053 GCAGATTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 194 CCGGG CC^[GC]GG
BpmI 1157 CTCCAG CTGGAG(16/14)
BpuAI 869 GTCTTC GAAGAC(2/6)
BsgI 812 GTGCAG GTGCAG(16/14)
BsiEI 157 CGACCG CG[GA][CT]^CG
BsmAI 797 GAGAC GTCTC(1/5)
BsmBI 797 GAGACG CGTCTC(1/5)
BsmI 566 GAATGC GAATGC(1/-1)
BspMI 16 ACCTGC ACCTGC(4/8)
BsrBI 1182 CCGCTC CCGCTC(-3/-3)
BsrDI 144 CATTGC GCAATG(2/0)
Bst1107I 436 GTATAC GTA^TAC
BstXI 1214 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 436 GTATAC GTA^TAC
DraI 1111 TTTAAA TTT^AAA
DraII 96 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 864 CCGTGG C^C[GA][CT]GG
EaeI 1244 TGGCCG [CT]^GGCC[GA]
EarI 292 CTCTTC CTCTTC(1/4)
EcoO109I 96 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 767 GAATTC G^AATTC
EcoT22I 554 ATGCAT ATGCA^T
FauI 148 GCGGG CCCGC(4/6)
Ksp632I 292 CTCTTC CTCTTC(1/4)
MaeII 74 ACGT A^CGT
NciI 194 CCGGG CC^[GC]GG
NsiI 554 ATGCAT ATGCA^T
PflMI 1228 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 96 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 134 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 47 CTGCAG CTGCA^G
PvuII 178 CAGCTG CAG^CTG
RsaI 38 GTAC GT^AC
SapI 291 GCTCTTC GCTCTTC(1/4)
ScaI 37 AGTACT AGT^ACT
SfcI 47 CTGCAG C^T[GA][CT]AG
SmlI 1185 CTCAAG C^T[CT][GA]AG
TaqI 132 TCGA T^CGA
TthHB8I 132 TCGA T^CGA
Van91I 1228 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 213 CCAGCTCTTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1125 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 33 CGCG CG^CG
155 CGCG
AcsI 421 GAATTT [GA]^AATT[CT]
767 GAATTC
ApoI 421 GAATTT [GA]^AATT[CT]
767 GAATTC
AvaII 97 GGTCC G^G[AT]CC
517 GGACC
BamHI 360 GGATCC G^GATCC
724 GGATCC
BanII 196 GGGCTC G[GA]GC[CT]^C
1224 GGGCCC
BbvI 180 GCTGC GCAGC(8/12)
678 GCTGC
BfaI 101 CTAG C^TAG
208 CTAG
BseRI 90 GAGGAG GAGGAG(10/8)
259 GAGGAG
BstUI 33 CGCG CG^CG
155 CGCG
HapII 194 CCGG C^CGG
1247 CCGG
HpaII 194 CCGG C^CGG
1247 CCGG
MaeI 101 CTAG C^TAG
208 CTAG
MspA1I 123 CAGCGG C[AC]G^C[GT]G
178 CAGCTG
MspI 194 CCGG C^CGG
1247 CCGG
MvnI 33 CGCG CG^CG
155 CGCG
SspI 274 AATATT AAT^ATT
463 AATATT
TseI 180 GCTGC G^C[AT]GC
678 GCTGC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
Tsp45I Tsp509I TspRI XhoII