(Circular) (Six-base) MAPSORT of: pFN21AB8667.seq Check: 2464 from: 1 to: 5977 pFN21AB8667 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2442 3320 1937 Size: 505 878 4594 Fragments arranged by size: 4594 878 505 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 4040 5424 5692 1632 Size: 1040 1368 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1040 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2188 3720 5292 2188 Size: 1532 1572 2873 Fragments arranged by size: 2873 1572 1532 ApaI G_GGCC'C Cuts at: 3233 3233 Size: 5977 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2426 2772 3286 3512 3664 5773 1493 Size: 933 346 514 226 152 2109 1697 Fragments arranged by size: 2109 1697 933 514 346 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 BamHI G'GATC_C Cuts at: 2365 2729 3307 3674 2365 Size: 364 578 367 4668 Fragments arranged by size: 4668 578 367 364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2205 3233 3296 1391 Size: 814 1028 63 4072 Fragments arranged by size: 4072 1028 814 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 928 Size: 971 968 4038 Fragments arranged by size: 4038 971 968 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3047 3081 4168 4202 1903 Size: 34 1110 34 1087 34 3678 Fragments arranged by size: 3678 1110 1087 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BfrBI ATG'CAT Cuts at: 2561 2561 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4588 136 Size: 107 122 71 631 605 337 2579 1525 Fragments arranged by size: 2579 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2159 3233 4038 5194 1790 Size: 369 1074 805 1156 2573 Fragments arranged by size: 2573 1156 1074 805 369 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 3708 4666 Size: 416 53 83 399 24 638 1653 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1653 958 638 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2904 2904 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3147 4637 1239 Size: 1908 1490 2579 Fragments arranged by size: 2579 1908 1490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3175 4102 4967 5265 3175 Size: 927 865 298 3887 Fragments arranged by size: 3887 927 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 2279 1186 Size: 924 169 4884 Fragments arranged by size: 4884 924 169 BseYI C'CCAG_C Cuts at: 2206 3226 5180 5676 2206 Size: 1020 1954 496 2507 Fragments arranged by size: 2507 1954 1020 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2838 1163 Size: 573 1102 4302 Fragments arranged by size: 4302 1102 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2165 3427 4188 5216 1540 Size: 275 186 164 1262 761 1028 2301 Fragments arranged by size: 2301 1262 1028 761 275 186 164 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 2577 3488 3581 2577 Size: 911 93 4973 Fragments arranged by size: 4973 911 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2796 2796 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2205 3233 3296 4038 4123 5194 Size: 399 369 46 1028 63 742 85 1071 Cuts at: 5194 1391 Size: 2174 Fragments arranged by size: 2174 1071 1028 742 399 369 85 63 46 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 3332 844 Size: 1186 1302 3489 Fragments arranged by size: 3489 1302 1186 BsrBI CCG'CTC Cuts at: 1394 1823 3189 3864 1394 Size: 429 1366 675 3507 Fragments arranged by size: 3507 1366 675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2148 4472 4646 65 Size: 1183 900 2324 174 1396 Fragments arranged by size: 2324 1396 1183 900 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 3226 3226 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2365 2729 3202 3307 3674 4059 4076 5528 Size: 393 364 473 105 367 385 17 1452 Cuts at: 5528 5614 5626 5972 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 473 393 385 367 364 346 105 86 17 12 BstZ17I GTA'TAC Cuts at: 2443 2443 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2004 2869 513 Size: 552 939 865 3621 Fragments arranged by size: 3621 939 865 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2019 2061 3493 4287 4307 2019 Size: 42 1432 794 20 3689 Fragments arranged by size: 3689 1432 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3118 3281 3118 Size: 163 5814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3249 3424 4313 Size: 54 1425 50 275 18 1419 175 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1419 889 275 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2303 3907 1353 Size: 950 1604 3423 Fragments arranged by size: 3423 1604 950 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3205 4564 4938 5084 387 Size: 1064 138 1616 1359 374 146 1280 Fragments arranged by size: 1616 1359 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2672 3147 4040 4637 5424 5692 1239 Size: 393 1040 475 893 597 787 268 1524 Fragments arranged by size: 1524 1040 893 787 597 475 393 268 EcoO109I rG'GnC_Cy Cuts at: 2102 2102 Size: 5977 EcoRI G'AATT_C Cuts at: 2772 3286 3664 2772 Size: 514 378 5085 Fragments arranged by size: 5085 514 378 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2615 2647 4618 Size: 32 135 32 514 32 497 32 1971 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1971 514 497 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 5091 5275 1305 Size: 919 2867 184 2007 Fragments arranged by size: 2867 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 2794 2909 3935 4294 4453 518 Size: 648 1387 241 115 1026 359 159 2042 Fragments arranged by size: 2042 1387 1026 648 359 241 159 115 MspA1I CmG'CkG Cuts at: 1606 2130 2185 3393 4070 5218 5463 1606 Size: 524 55 1208 677 1148 245 2120 Fragments arranged by size: 2120 1208 1148 677 524 245 55 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NsiI A_TGCA'T Cuts at: 2563 2563 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3335 4880 1132 Size: 97 2106 1545 2229 Fragments arranged by size: 2229 2106 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 3239 1108 Size: 2131 3846 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2102 2102 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 2143 1613 Size: 530 5447 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 3229 3229 Size: 5977 PstI C_TGCA'G Cuts at: 830 2056 3329 830 Size: 1226 1273 3478 Fragments arranged by size: 3478 1273 1226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2185 2185 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2303 2303 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 2044 4225 5937 2044 Size: 2181 1712 2084 Fragments arranged by size: 2181 2084 1712 SfcI C'TryA_G Cuts at: 826 1045 2052 3325 4460 5141 5332 826 Size: 219 1007 1273 1135 681 191 1471 Fragments arranged by size: 1471 1273 1135 1007 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4081 4982 5244 820 Size: 197 926 1247 891 901 262 1553 Fragments arranged by size: 1553 1247 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 2281 2470 3901 5 Size: 47 2229 189 1431 2081 Fragments arranged by size: 2229 2081 1431 189 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 3416 513 Size: 552 939 1412 3074 Fragments arranged by size: 3074 1412 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2042 4223 5935 Size: 274 80 33 51 1443 65 2181 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 2181 1712 1443 274 138 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2910 2942 5937 5969 2910 Size: 32 2995 32 2918 Fragments arranged by size: 2995 2918 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2225 2011 Size: 214 5763 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 3134 4106 3134 Size: 972 5005 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI