Restriction map for
pFN21AB8666
(Circular) (Six-base) MAPSORT of: pFN21AB8666.seq Check: 3057 from: 1 to: 5791
pFN21AB8666 5791 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2774 3854 5238 5506 1632
Size: 815 327 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1080 815 327 268
AfeI AGC'GCT
Cuts at: 2351 2351
Size: 5791
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 2974 4690 5667 1203
Size: 1771 1716 977 1327
Fragments arranged by size:
1771 1716 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2425 2860 3534 5106 2425
Size: 435 674 1572 3110
Fragments arranged by size:
3110 1572 674 435
ApaI G_GGCC'C
Cuts at: 2551 2551
Size: 5791
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 2402 2672 3100 3326 3478 5587 1493
Size: 909 270 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 428 270 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 3116 1870
Size: 73 586 587 4545
Fragments arranged by size:
4545 587 586 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2551 2745 3110 1391
Size: 1160 194 365 4072
Fragments arranged by size:
4072 1160 365 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 2453 928
Size: 971 333 221 4266
Fragments arranged by size:
4266 971 333 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2216 2551 3852 5008 1790
Size: 426 335 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 426 335
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2614 4451 1239
Size: 1375 1837 2579
Fragments arranged by size:
2579 1837 1375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2864 4454 882
Size: 617 1365 1590 2219
Fragments arranged by size:
2219 1590 1365 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3598 3980 4615 275
Size: 53 83 186 936 2065 382 635 1451
Fragments arranged by size:
2065 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2587 2617 2629 2659 2867
Size: 30 165 30 1184 30 12 30 208
Cuts at: 2867 2897 1178
Size: 30 4072
Fragments arranged by size:
4072 1184 208 165 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3023 1186
Size: 1837 3954
BseYI C'CCAG_C
Cuts at: 4994 5490 4994
Size: 496 5295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2873 2952 1163
Size: 573 1137 79 4002
Fragments arranged by size:
4002 1137 573 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2720 2745 2885 3852 3937 5008 2720
Size: 25 140 967 85 1071 3503
Fragments arranged by size:
3503 1071 967 140 85 25
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2551 2720 2745 2885 3110 3852
Size: 399 426 335 169 25 140 225 742
Cuts at: 3852 3937 5008 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 335 225 169
140 85 25
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3146 844
Size: 2302 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 3678 1394
Size: 429 1237 618 3507
Fragments arranged by size:
3507 1237 618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 2876 3851 4863 2876
Size: 975 1012 3804
Fragments arranged by size:
3804 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2054 2715 2860 794
Size: 1260 661 145 3725
Fragments arranged by size:
3725 1260 661 145
BstBI TT'CG_AA
Cuts at: 2098 4595 2098
Size: 2497 3294
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2893 2953 2893
Size: 60 5731
BstYI r'GATC_y
Cuts at: 1972 3121 3488 3873 3890 5342 5428 5440 5786
Size: 1149 367 385 17 1452 86 12 346
Cuts at: 5786 1972
Size: 1977
Fragments arranged by size:
1977 1452 1149 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2418 2630 2418
Size: 212 5579
BtgI C'CryG_G
Cuts at: 513 1065 3018 513
Size: 552 1953 3286
Fragments arranged by size:
3286 1953 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 4588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2769 2812 3238
Size: 54 1425 50 275 18 939 43 426
Cuts at: 3238 4127 5756 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 939 889 426 275 54 50
43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 2823 3721 1353
Size: 1380 90 898 3423
Fragments arranged by size:
3423 1380 898 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 2743
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2614 2774 3854 4451 5238 5506
Size: 393 815 167 160 1080 597 787 268
Cuts at: 5506 1239
Size: 1524
Fragments arranged by size:
1524 1080 815 787 597 393 268 167
160
EcoO109I rG'GnC_Cy
Cuts at: 2547 2547
Size: 5791
EcoRI G'AATT_C
Cuts at: 2402 3100 3478 2402
Size: 698 378 4715
Fragments arranged by size:
4715 698 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2477 2477
Size: 5791
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2353 4938 1678
Size: 675 2585 2531
Fragments arranged by size:
2585 2531 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 4432 4464 4506
Size: 32 135 32 566 32 2262 32 42
Cuts at: 4506 4538 1373
Size: 32 2626
Fragments arranged by size:
2626 2262 566 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2271 3135 3381 669
Size: 988 281 333 864 246 3079
Fragments arranged by size:
3079 988 864 333 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 4905 5089 1305
Size: 1473 2127 184 2007
Fragments arranged by size:
2127 2007 1473 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 2814 10
Size: 54 1425 343 939 43 2987
Fragments arranged by size:
2987 1425 939 343 54 43
MslI CAynn'nnrTG
Cuts at: 518 1166 3749 4108 4267 518
Size: 648 2583 359 159 2042
Fragments arranged by size:
2583 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2909 3207 3884 5032 5277 1606
Size: 1303 298 677 1148 245 2120
Fragments arranged by size:
2120 1303 1148 677 298 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5239
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2460 2978 3149 4694 1132
Size: 97 1231 518 171 1545 2229
Fragments arranged by size:
2229 1545 1231 518 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 2974 4690 2974
Size: 1716 4075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspOMI G'GGCC_C
Cuts at: 2547 2547
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SacI G_AGCT'C
Cuts at: 2745 2745
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SapI GCTCTTCn'nnn_
Cuts at: 2733 2733
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2506 3139 4274 4955 5146 826
Size: 219 1461 633 1135 681 191 1471
Fragments arranged by size:
1471 1461 1135 681 633 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 3031 1517
Size: 1514 4277
StyI C'CwwG_G
Cuts at: 513 1065 3230 513
Size: 552 2165 3074
Fragments arranged by size:
3074 2165 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3818 3835 3988 4173 4618 325
Size: 1255 1338 900 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 900 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2134 4037 5749
Size: 274 80 33 51 1443 157 1903 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1903 1712 1443 274 157 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2587 2619 2867 2899 2964 2996 5751 5783 2587
Size: 32 248 32 65 32 2755 32 2595
Fragments arranged by size:
2755 2595 248 65 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 2104 2244 3920 2104
Size: 140 1676 3975
Fragments arranged by size:
3975 1676 140
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI