Restriction map for
pFN21AB8663
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MspA1I MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1634 CGCG CG^CG
AocI 1549 CCTGAGG CC^T[ACGT]AGG
AvaII 1104 GGACC G^G[AT]CC
BalI 1619 TGGCCA TGG^CCA
BbsI 1335 GTCTTC GAAGAC(2/6)
BclI 1197 TGATCA T^GATCA
BcnI 10 CCGGG CC^[GC]GG
BmyI 889 GGGCAC G[AGT]GC[ACT]^C
BpuAI 1335 GTCTTC GAAGAC(2/6)
BsaJI 1093 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 1010 ACCGGA [AT]^CCGG[AT]
BsgI 497 CTGCAC GTGCAG(16/14)
BsiYI 132 CCTTCAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 132 CCTTCAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1103 GGGAC GGGAC(10/14)
BsmI 695 GCATTC GAATGC(1/-1)
Bsp1286I 889 GGGCAC G[AGT]GC[ACT]^C
BstAPI 199 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 869 GGTCACC G^GT[ACGT]ACC
BstPI 869 GGTCACC G^GT[ACGT]ACC
BstUI 1634 CGCG CG^CG
BstXI 1622 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1549 CCTGAGG CC^T[ACGT]AGG
CfoI 1633 GCGC GCG^C
DraII 191 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 872 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1619 TGGCCA [CT]^GGCC[GA]
Eco81I 1549 CCTGAGG CC^T[ACGT]AGG
EcoO109I 191 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 869 GGTCACC G^GT[ACGT]ACC
EcoT14I 1093 CCAAGG C^C[AT][AT]GG
EcoT22I 693 ATGCAT ATGCA^T
FauI 1635 GCGGG CCCGC(4/6)
FbaI 1197 TGATCA T^GATCA
HhaI 1633 GCGC GCG^C
HinP1I 1633 GCGC G^CGC
MaeII 925 ACGT A^CGT
MluNI 1619 TGGCCA TGG^CCA
MscI 1619 TGGCCA TGG^CCA
MvnI 1634 CGCG CG^CG
NciI 10 CCGGG CC^[GC]GG
NdeI 1258 CATATG CA^TATG
NsiI 693 ATGCAT ATGCA^T
NspI 508 GCATGT [GA]CATG^[CT]
PstI 798 CTGCAG CTGCA^G
SapI 1252 GAAGAGC GCTCTTC(1/4)
SfcI 798 CTGCAG C^T[GA][CT]AG
SspI 1157 AATATT AAT^ATT
StyI 1093 CCAAGG C^C[AT][AT]GG
XcmI 272 CCAAATTGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 1374 AAATTC [GA]^AATT[CT]
1393 AAATTT
AlwI 1412 GATCC GGATC(4/5)
916 GGATC
ApoI 1374 AAATTC [GA]^AATT[CT]
1393 AAATTT
Asp700I 127 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
831 GAATGACTTC
BanI 554 GGCACC G^G[CT][GA]CC
902 GGCACC
BglII 534 AGATCT A^GATCT
616 AGATCT
BstF5I 516 GGATG GGATG(2/0)
768 GGATG
BstNI 899 CCAGG CC^[AT]GG
1622 CCAGG
Cac8I 14 GCAGGC GC[ACGT]^[ACGT]GC
156 GCAGGC
Cfr13I 192 GGCCC G^G[ACGT]CC
1104 GGACC
EcoRII 899 CCAGG ^CC[AT]GG
1622 CCAGG
FokI 516 GGATG GGATG(9/13)
768 GGATG
HapII 10 CCGG C^CGG
1011 CCGG
HpaII 10 CCGG C^CGG
1011 CCGG
HphI 577 GGTGA GGTGA(8/7)
871 TCACC
MseI 562 TTAA T^TAA
1640 TTAA
MspI 10 CCGG C^CGG
1011 CCGG
MvaI 899 CCAGG CC^[AT]GG
1622 CCAGG
PleI 1502 GACTC GAGTC(4/5)
852 GAGTC
Sau96I 192 GGCCC G^G[ACGT]CC
1104 GGACC
TaqI 749 TCGA T^CGA
928 TCGA
Tru9I 562 TTAA T^TAA
1640 TTAA
TthHB8I 749 TCGA T^CGA
928 TCGA
XmnI 127 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
831 GAATGACTTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsaI BsmAI BsrI BssKI BstYI
DdeI DpnII EarI Fnu4HI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI TfiI TseI Tsp45I
Tsp509I TspRI XhoII