(Circular) (Six-base) MAPSORT of: pFN21AB8663.seq Check: 4719 from: 1 to: 6379 pFN21AB8663 6379 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2493 3327 4442 5826 6094 1632 Size: 491 370 834 1115 1384 268 1917 Fragments arranged by size: 1917 1384 1115 834 491 370 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 5278 6255 1203 Size: 4075 977 1327 Fragments arranged by size: 4075 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6379 AlwNI CAG_nnn'CTG Cuts at: 4122 5694 4122 Size: 1572 4807 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 3379 3398 3688 3914 4066 6175 1493 Size: 1886 19 290 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 290 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 3704 1870 Size: 73 1761 4545 Fragments arranged by size: 4545 1761 73 BamHI G'GATC_C Cuts at: 3709 4076 3709 Size: 367 6012 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 2907 3700 5155 618 Size: 325 217 627 772 348 793 1455 1842 Fragments arranged by size: 1842 1455 793 772 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 3698 1391 Size: 2307 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3333 928 Size: 971 1434 3974 Fragments arranged by size: 3974 1434 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4271 5481 4271 Size: 1210 5169 BclI T'GATC_A Cuts at: 1548 3202 1548 Size: 1654 4725 BfrBI ATG'CAT Cuts at: 2700 2700 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4990 136 Size: 107 122 71 631 605 3318 1525 Fragments arranged by size: 3318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2539 2621 6374 2539 Size: 82 3753 2544 Fragments arranged by size: 3753 2544 82 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 2898 4440 5596 1790 Size: 1108 1542 1156 2573 Fragments arranged by size: 2573 1542 1156 1108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 3500 4110 Size: 416 53 83 399 24 1793 290 610 Cuts at: 4110 5068 442 Size: 958 1753 Fragments arranged by size: 1793 1753 958 610 416 399 290 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3487 3487 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5039 1239 Size: 3800 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4504 5369 5667 4504 Size: 865 298 5216 Fragments arranged by size: 5216 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 2819 2976 3541 5042 882 Size: 617 612 708 157 565 1501 2219 Fragments arranged by size: 2219 1501 708 617 612 565 157 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3015 4812 5210 5484 5631 6042 3015 Size: 1797 398 274 147 411 3352 Fragments arranged by size: 3352 1797 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6154 Fragments arranged by size: 6154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6379 BseYI C'CCAG_C Cuts at: 5582 6078 5582 Size: 496 5883 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2487 1163 Size: 573 751 5055 Fragments arranged by size: 5055 751 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4440 4525 5596 4440 Size: 85 1071 5223 Fragments arranged by size: 5223 1071 85 BsmI GAATG_Cn' Cuts at: 2700 3890 3983 2700 Size: 1190 93 5096 Fragments arranged by size: 5096 1190 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2898 3698 4440 4525 5596 1391 Size: 399 1108 800 742 85 1071 2174 Fragments arranged by size: 2174 1108 1071 800 742 399 85 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 844 Size: 2890 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4874 5048 65 Size: 1183 3626 174 1396 Fragments arranged by size: 3626 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2210 794 Size: 1416 4963 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 2874 1184 Size: 1690 4689 BstXI CCAn_nnnn'nTGG Cuts at: 3634 3634 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2539 2621 3416 3709 4076 4461 4478 5930 Size: 567 82 795 293 367 385 17 1452 Cuts at: 5930 6016 6028 6374 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 795 567 385 367 346 293 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 3555 3555 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2773 3567 3895 4689 4709 2773 Size: 794 328 794 20 4443 Fragments arranged by size: 4443 794 794 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DraIII CAC_nnn'GTG Cuts at: 2882 2882 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3624 3826 4715 Size: 54 1425 50 275 18 1794 202 889 Cuts at: 4715 6344 8 Size: 1629 43 Fragments arranged by size: 1794 1629 1425 889 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 3068 3231 3252 4309 1353 Size: 1227 488 163 21 1057 3423 Fragments arranged by size: 3423 1227 1057 488 163 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2493 3327 4442 5039 5826 6094 Size: 393 491 370 834 1115 597 787 268 Cuts at: 6094 1239 Size: 1524 Fragments arranged by size: 1524 1115 834 787 597 491 393 370 268 EcoO109I rG'GnC_Cy Cuts at: 2197 2197 Size: 6379 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 5020 5052 5094 Size: 32 135 32 533 32 2883 32 42 Cuts at: 5094 5126 1373 Size: 32 2626 Fragments arranged by size: 2883 2626 533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3723 3969 669 Size: 988 281 1785 246 3079 Fragments arranged by size: 3079 1785 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3444 5493 5677 1305 Size: 2139 2049 184 2007 Fragments arranged by size: 2139 2049 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3626 10 Size: 54 1425 343 1794 2763 Fragments arranged by size: 2763 1794 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4337 4696 4855 518 Size: 648 3171 359 159 2042 Fragments arranged by size: 3171 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3795 4472 5620 5865 1606 Size: 2189 677 1148 245 2120 Fragments arranged by size: 2189 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 NdeI CA'TA_TG Cuts at: 387 3264 387 Size: 2877 3502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NsiI A_TGCA'T Cuts at: 2702 2702 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2517 3737 5282 1132 Size: 97 1288 1220 1545 2229 Fragments arranged by size: 2229 1545 1288 1220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6379 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PstI C_TGCA'G Cuts at: 830 2807 3731 830 Size: 1977 924 3478 Fragments arranged by size: 3478 1977 924 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SapI GCTCTTCn'nnn_ Cuts at: 3252 3252 Size: 6379 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 2803 3727 4862 5543 5734 826 Size: 219 1758 924 1135 681 191 1471 Fragments arranged by size: 1758 1471 1135 924 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4483 5384 5646 820 Size: 197 926 2540 901 262 1553 Fragments arranged by size: 2540 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 3164 4303 5 Size: 47 3112 1139 2081 Fragments arranged by size: 3112 2081 1139 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2004 3098 3818 513 Size: 552 939 1094 720 3074 Fragments arranged by size: 3074 1094 939 720 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2862 4406 4423 4576 4761 5206 325 Size: 1255 1282 1544 17 153 185 445 1498 Fragments arranged by size: 1544 1498 1282 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4625 6337 96 Size: 274 80 33 51 1443 2648 1712 138 Fragments arranged by size: 2648 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6339 6371 6339 Size: 32 6347 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 2136 2840 4508 2136 Size: 704 1668 4007 Fragments arranged by size: 4007 1668 704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI