Restriction map for
pFN21AB8663
(Linear) (Six-base) MAPSORT of: KIBB8663.seq Check: 9455 from: 1 to: 1674
KIBB8663.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 488 1322 1674
Size: 118 370 834 352
Fragments arranged by size:
834 370 352 118
ApoI r'AATT_y
Cuts at: 0 1374 1393 1674
Size: 1374 19 281
Fragments arranged by size:
1374 281 19
BanI G'GyrC_C
Cuts at: 0 554 902 1674
Size: 554 348 772
Fragments arranged by size:
772 554 348
BbsI GAAGACnn'nnnn_
Cuts at: 0 1328 1674
Size: 1328 346
BclI T'GATC_A
Cuts at: 0 1197 1674
Size: 1197 477
BfrBI ATG'CAT
Cuts at: 0 695 1674
Size: 695 979
BglII A'GATC_T
Cuts at: 0 534 616 1674
Size: 534 82 1058
Fragments arranged by size:
1058 534 82
Bme1580I G_kGCm'C
Cuts at: 0 893 1674
Size: 893 781
BmrI ACTGGGnnnn_n'
Cuts at: 0 1205 1495 1674
Size: 1205 290 179
Fragments arranged by size:
1205 290 179
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1482 1674
Size: 1482 192
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 814 971 1536 1674
Size: 106 708 157 565 138
Fragments arranged by size:
708 565 157 138 106
BsaWI w'CCGG_w
Cuts at: 0 1010 1674
Size: 1010 664
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1674
Size: 482 1192
BsmI GAATG_Cn'
Cuts at: 0 695 1674
Size: 695 979
Bsp1286I G_dGCh'C
Cuts at: 0 893 1674
Size: 893 781
BstAPI GCAn_nnn'nTGC
Cuts at: 0 205 1674
Size: 205 1469
BstEII G'GTnAC_C
Cuts at: 0 869 1674
Size: 869 805
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1629 1674
Size: 1629 45
BstYI r'GATC_y
Cuts at: 0 534 616 1411 1674
Size: 534 82 795 263
Fragments arranged by size:
795 534 263 82
Bsu36I CC'TnA_GG
Cuts at: 0 1550 1674
Size: 1550 124
BtsI GCAGTG_nn'
Cuts at: 0 768 1562 1674
Size: 768 794 112
Fragments arranged by size:
794 768 112
DraIII CAC_nnn'GTG
Cuts at: 0 877 1674
Size: 877 797
EaeI y'GGCC_r
Cuts at: 0 1619 1674
Size: 1619 55
EarI CTCTTCn'nnn_
Cuts at: 0 575 1063 1226 1247 1674
Size: 575 488 163 21 427
Fragments arranged by size:
575 488 427 163 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 488 1322 1674
Size: 118 370 834 352
Fragments arranged by size:
834 370 352 118
EcoO109I rG'GnC_Cy
Cuts at: 0 192 1674
Size: 192 1482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1674
Size: 100 32 1542
Fragments arranged by size:
1542 100 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 1674
Size: 1439 235
MscI TGG'CCA
Cuts at: 0 1621 1674
Size: 1621 53
NdeI CA'TA_TG
Cuts at: 0 1259 1674
Size: 1259 415
NsiI A_TGCA'T
Cuts at: 0 697 1674
Size: 697 977
NspI r_CATG'y
Cuts at: 0 512 1674
Size: 512 1162
PstI C_TGCA'G
Cuts at: 0 802 1674
Size: 802 872
SapI GCTCTTCn'nnn_
Cuts at: 0 1247 1674
Size: 1247 427
SfcI C'TryA_G
Cuts at: 0 798 1674
Size: 798 876
SspI AAT'ATT
Cuts at: 0 1159 1674
Size: 1159 515
StyI C'CwwG_G
Cuts at: 0 1093 1674
Size: 1093 581
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 857 1674
Size: 857 817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1674
Size: 279 1395
XmnI GAAnn'nnTTC
Cuts at: 0 131 835 1674
Size: 131 704 839
Fragments arranged by size:
839 704 131
Enzymes that do cut:
AcuI ApoI BanI BbsI BclI BfrBI BglII Bme1580I
BmrI BplI BsaI BsaWI BsgI BsmI Bsp1286I BstAPI
BstEII BstXI BstYI Bsu36I BtsI DraIII EaeI EarI
Eco57MI EcoO109I Hin4I MmeI MscI NdeI NsiI NspI
PstI SapI SfcI SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BciVI BglI BlpI BmgBI BmtI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI