Restriction map for
pFN21AB8662
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BlnI BpuAI BsaAI BsaHI BsaI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeI
MaeIII MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 370 CGCG CG^CG
AccIII 926 TCCGGA T^CCGGA
AcsI 410 AAATTC [GA]^AATT[CT]
AfaI 310 GTAC GT^AC
ApoI 410 AAATTC [GA]^AATT[CT]
AspHI 94 GAGCTC G[AT]GC[AT]^C
BalI 497 TGGCCA TGG^CCA
BanI 951 GGCACC G^G[CT][GA]CC
BcgI 431 CGAGAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 96 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 96 GCTCAGC GC^T[ACGT]AGC
BsaBI 245 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 926 TCCGGA [AT]^CCGG[AT]
BseAI 926 TCCGGA T^CCGGA
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BspEI 926 TCCGGA T^CCGGA
BsrDI 799 GCAATG GCAATG(2/0)
BsrI 159 ACTGG ACTGG(1/-1)
BssSI 385 CACGAG CACGAG(-5/-1)
BstAPI 214 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 370 CGCG CG^CG
CelII 96 GCTCAGC GC^T[ACGT]AGC
DraII 644 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 270 CCGTGG C^C[GA][CT]GG
EcoO109I 644 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 80 GCGGG CCCGC(4/6)
HgaI 23 GCGTC GACGC(5/10)
HindIII 787 AAGCTT A^AGCTT
MamI 245 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 497 TGGCCA TGG^CCA
MroI 926 TCCGGA T^CCGGA
MscI 497 TGGCCA TGG^CCA
MslI 1011 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 370 CGCG CG^CG
PshAI 966 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 310 GTAC GT^AC
SacI 94 GAGCTC GAGCT^C
SapI 1005 GAAGAGC GCTCTTC(1/4)
SspI 146 AATATT AAT^ATT
StuI 279 AGGCCT AGG^CCT
TspRI 911 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 245 GATCC GGATC(4/5)
171 GGATC
BanII 94 GAGCTC G[GA]GC[CT]^C
163 GAGCCC
BglII 830 AGATCT A^GATCT
983 AGATCT
BsmAI 127 GAGAC GTCTC(1/5)
700 GAGAC
BspMI 374 ACCTGC ACCTGC(4/8)
762 GCAGGT
BstF5I 336 GGATG GGATG(2/0)
1016 GGATG
BstNI 167 CCAGG CC^[AT]GG
999 CCTGG
EaeI 497 TGGCCA [CT]^GGCC[GA]
938 TGGCCG
EarI 631 GAAGAG CTCTTC(1/4)
1005 GAAGAG
EcoRII 167 CCAGG ^CC[AT]GG
999 CCTGG
FokI 336 GGATG GGATG(9/13)
1016 GGATG
HphI 636 GGTGA GGTGA(8/7)
960 GGTGA
Ksp632I 631 GAAGAG CTCTTC(1/4)
1005 GAAGAG
MaeII 567 ACGT A^CGT
971 ACGT
MfeI 616 CAATTG C^AATTG
704 CAATTG
MspA1I 73 CAGCTG C[AC]G^C[GT]G
934 CAGCTG
MunI 616 CAATTG C^AATTG
704 CAATTG
MvaI 167 CCAGG CC^[AT]GG
999 CCTGG
PleI 34 GACTC GAGTC(4/5)
13 GAGTC
PstI 376 CTGCAG CTGCA^G
760 CTGCAG
PvuII 73 CAGCTG CAG^CTG
934 CAGCTG
SfaNI 381 GCATC GCATC(5/9)
1010 GCATC
SfcI 376 CTGCAG C^T[GA][CT]AG
760 CTGCAG
SmlI 301 CTTGAG C^T[CT][GA]AG
697 CTTGAG
TfiI 717 GAATC G^A[AT]TC
1077 GAATC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstYI Cac8I
Cfr13I DdeI DpnII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MflI MnlI MseI MspI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI Tsp509I TthHB8I XhoII