(Circular) (Six-base) MAPSORT of: pFN21AB8662.seq Check: 6458 from: 1 to: 5836 pFN21AB8662 5836 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 3579 5151 3579 Size: 1572 4264 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2415 3145 3371 3523 5632 1493 Size: 922 730 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 730 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 3161 1870 Size: 73 1218 4545 Fragments arranged by size: 4545 1218 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 3157 4612 618 Size: 325 217 627 1169 201 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 201 BanII G_rGCy'C Cuts at: 1391 2103 2172 3155 1391 Size: 712 69 983 4072 Fragments arranged by size: 4072 983 712 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2425 2459 4027 4061 1903 Size: 34 488 34 1568 34 3678 Fragments arranged by size: 3678 1568 488 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2835 2988 5831 2835 Size: 153 2843 2840 Fragments arranged by size: 2843 2840 153 BlpI GC'TnA_GC Cuts at: 2102 3253 2102 Size: 1151 4685 Bme1580I G_kGCm'C Cuts at: 1790 2959 3897 5053 1790 Size: 1169 938 1156 2573 Fragments arranged by size: 2573 1169 1156 938 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2186 3026 4496 1239 Size: 842 105 840 1470 2579 Fragments arranged by size: 2579 1470 842 840 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 2723 3961 4826 5124 2327 Size: 396 1238 865 298 3039 Fragments arranged by size: 3039 1238 865 396 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 2254 5830 2254 Size: 3576 2260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2931 4269 4667 4941 5088 5499 2931 Size: 1338 398 274 147 411 3268 Fragments arranged by size: 3268 1338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5611 Fragments arranged by size: 5611 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2420 2906 1186 Size: 925 309 486 4116 Fragments arranged by size: 4116 925 486 309 BseYI C'CCAG_C Cuts at: 2736 3128 5039 5535 2736 Size: 392 1911 496 3037 Fragments arranged by size: 3037 1911 496 392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 3897 3982 5053 2103 Size: 1794 85 1071 2886 Fragments arranged by size: 2886 1794 1071 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2172 2959 3155 3897 3982 5053 Size: 399 313 69 787 196 742 85 1071 Cuts at: 5053 1391 Size: 2174 Fragments arranged by size: 2174 1071 787 742 399 313 196 85 69 BspEI T'CCGG_A Cuts at: 2931 5499 2931 Size: 2568 3268 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2758 3191 844 Size: 1544 370 433 3489 Fragments arranged by size: 3489 1544 433 370 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 4331 4505 65 Size: 1183 1563 1520 174 1396 Fragments arranged by size: 1563 1520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2390 3896 4908 2390 Size: 1506 1012 3318 Fragments arranged by size: 3318 1506 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2225 794 Size: 1431 4405 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2249 2835 2988 3166 3533 3918 3935 5387 Size: 277 586 153 178 367 385 17 1452 Cuts at: 5387 5473 5485 5831 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 586 385 367 346 277 178 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2275 513 Size: 552 1210 4074 Fragments arranged by size: 4074 1210 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2922 3352 4146 4166 2922 Size: 430 794 20 4592 Fragments arranged by size: 4592 794 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2502 2943 3283 Size: 54 1425 50 275 18 672 441 340 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 672 441 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 3005 3766 1353 Size: 1278 374 761 3423 Fragments arranged by size: 3423 1278 761 374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2101 2101 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2186 3026 3899 4496 5283 5551 Size: 393 449 105 840 873 597 787 268 Cuts at: 5551 1239 Size: 1524 Fragments arranged by size: 1524 873 840 787 597 449 393 268 105 EcoO109I rG'GnC_Cy Cuts at: 2650 2650 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 2854 2873 2362 Size: 492 19 5325 Fragments arranged by size: 5325 492 19 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 4477 4509 4551 Size: 32 135 32 431 32 2442 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 2442 431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 748 Size: 2044 3792 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 2621 2709 3435 2621 Size: 88 726 5022 Fragments arranged by size: 5022 726 88 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 2061 4950 5134 1305 Size: 729 27 2889 184 2007 Fragments arranged by size: 2889 2007 729 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2504 10 Size: 54 1425 343 672 3342 Fragments arranged by size: 3342 1425 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3794 4153 4312 518 Size: 648 1854 774 359 159 2042 Fragments arranged by size: 2042 1854 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2941 3252 3929 5077 5322 1606 Size: 474 861 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 861 677 474 311 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 2172 1348 Size: 824 5012 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 2258 2979 2258 Size: 721 5115 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2975 1613 Size: 1362 4474 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 2385 2769 3188 830 Size: 1555 384 419 3478 Fragments arranged by size: 3478 1555 419 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2080 2941 2080 Size: 861 4975 SacI G_AGCT'C Cuts at: 2103 2103 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 3005 3005 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2381 2765 3184 4319 5000 5191 826 Size: 219 1336 384 419 1135 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 419 384 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2702 3940 4841 5103 820 Size: 197 926 363 396 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 396 363 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 2153 3760 5 Size: 47 2101 1607 2081 Fragments arranged by size: 2101 2081 1607 47 StuI AGG'CCT Cuts at: 1517 2286 1517 Size: 769 5067 StyI C'CwwG_G Cuts at: 513 1065 3275 513 Size: 552 2210 3074 Fragments arranged by size: 3074 2210 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI