Restriction map for
pFN21AB8662
(Linear) (Six-base) MAPSORT of: KIBB8662.seq Check: 9421 from: 1 to: 1131
KIBB8662.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
ApoI r'AATT_y
Cuts at: 0 410 1131
Size: 410 721
BanI G'GyrC_C
Cuts at: 0 951 1131
Size: 951 180
BanII G_rGCy'C
Cuts at: 0 98 167 1131
Size: 98 69 964
Fragments arranged by size:
964 98 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 420 454 1131
Size: 420 34 677
Fragments arranged by size:
677 420 34
BglII A'GATC_T
Cuts at: 0 830 983 1131
Size: 830 153 148
Fragments arranged by size:
830 153 148
BlpI GC'TnA_GC
Cuts at: 0 97 1131
Size: 97 1034
Bme1580I G_kGCm'C
Cuts at: 0 954 1131
Size: 954 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 181 1021 1131
Size: 76 105 840 110
Fragments arranged by size:
840 110 105 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 718 1131
Size: 322 396 413
Fragments arranged by size:
413 396 322
BsaBI GATnn'nnATC
Cuts at: 0 249 1131
Size: 249 882
BsaWI w'CCGG_w
Cuts at: 0 926 1131
Size: 926 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 415 901 1131
Size: 106 309 486 230
Fragments arranged by size:
486 309 230 106
BseYI C'CCAG_C
Cuts at: 0 731 1123 1131
Size: 731 392 8
Fragments arranged by size:
731 392 8
BsiHKAI G_wGCw'C
Cuts at: 0 98 1131
Size: 98 1033
Bsp1286I G_dGCh'C
Cuts at: 0 98 167 954 1131
Size: 98 69 787 177
Fragments arranged by size:
787 177 98 69
BspEI T'CCGG_A
Cuts at: 0 926 1131
Size: 926 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 753 1131
Size: 383 370 378
Fragments arranged by size:
383 378 370
BsrDI GCAATG_nn'
Cuts at: 0 806 1131
Size: 806 325
BssSI C'ACGA_G
Cuts at: 0 385 1131
Size: 385 746
BstAPI GCAn_nnn'nTGC
Cuts at: 0 220 1131
Size: 220 911
BstYI r'GATC_y
Cuts at: 0 244 830 983 1131
Size: 244 586 153 148
Fragments arranged by size:
586 244 153 148
BtgI C'CryG_G
Cuts at: 0 270 1131
Size: 270 861
BtsI GCAGTG_nn'
Cuts at: 0 917 1131
Size: 917 214
EaeI y'GGCC_r
Cuts at: 0 497 938 1131
Size: 497 441 193
Fragments arranged by size:
497 441 193
EarI CTCTTCn'nnn_
Cuts at: 0 626 1000 1131
Size: 626 374 131
Fragments arranged by size:
626 374 131
EcoICRI GAG'CTC
Cuts at: 0 96 1131
Size: 96 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 181 1021 1131
Size: 76 105 840 110
Fragments arranged by size:
840 110 105 76
EcoO109I rG'GnC_Cy
Cuts at: 0 645 1131
Size: 645 486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 849 868 1131
Size: 357 492 19 263
Fragments arranged by size:
492 357 263 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1131
Size: 30 1101
HindIII A'AGCT_T
Cuts at: 0 787 1131
Size: 787 344
MfeI C'AATT_G
Cuts at: 0 616 704 1131
Size: 616 88 427
Fragments arranged by size:
616 427 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 56 1131
Size: 29 27 1075
Fragments arranged by size:
1075 29 27
MscI TGG'CCA
Cuts at: 0 499 1131
Size: 499 632
MslI CAynn'nnrTG
Cuts at: 0 1015 1131
Size: 1015 116
MspA1I CmG'CkG
Cuts at: 0 75 936 1131
Size: 75 861 195
Fragments arranged by size:
861 195 75
PasI CC'CwG_GG
Cuts at: 0 167 1131
Size: 167 964
PfoI T'CCnGG_A
Cuts at: 0 253 974 1131
Size: 253 721 157
Fragments arranged by size:
721 253 157
PshAI GACnn'nnGTC
Cuts at: 0 970 1131
Size: 970 161
PstI C_TGCA'G
Cuts at: 0 380 764 1131
Size: 380 384 367
Fragments arranged by size:
384 380 367
PvuII CAG'CTG
Cuts at: 0 75 936 1131
Size: 75 861 195
Fragments arranged by size:
861 195 75
SacI G_AGCT'C
Cuts at: 0 98 1131
Size: 98 1033
SapI GCTCTTCn'nnn_
Cuts at: 0 1000 1131
Size: 1000 131
SfcI C'TryA_G
Cuts at: 0 376 760 1131
Size: 376 384 371
Fragments arranged by size:
384 376 371
SmlI C'TyrA_G
Cuts at: 0 301 697 1131
Size: 301 396 434
Fragments arranged by size:
434 396 301
SspI AAT'ATT
Cuts at: 0 148 1131
Size: 148 983
StuI AGG'CCT
Cuts at: 0 281 1131
Size: 281 850
Enzymes that do cut:
ApoI BanI BanII BcgI BglII BlpI Bme1580I BpmI
BpuEI BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspMI BsrDI BssSI BstAPI BstYI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII MfeI
MmeI MscI MslI MspA1I PasI PfoI PshAI PstI
PvuII SacI SapI SfcI SmlI SspI StuI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI