(Circular) (Six-base) MAPSORT of: pFN21AB8650.seq Check: 9541 from: 1 to: 5695 pFN21AB8650 5695 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 3038 1937 Size: 454 647 4594 Fragments arranged by size: 4594 647 454 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2022 3758 5142 5410 1632 Size: 390 1736 1384 268 1917 Fragments arranged by size: 1917 1736 1384 390 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 2491 4424 2491 Size: 1933 3762 AleI CACnn'nnGTG Cuts at: 1166 2514 1166 Size: 1348 4347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2644 3438 5010 2644 Size: 794 1572 3329 Fragments arranged by size: 3329 1572 794 ApaI G_GGCC'C Cuts at: 2833 2833 Size: 5695 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 2149 2407 3004 3230 3382 5491 1493 Size: 656 258 597 226 152 2109 1697 Fragments arranged by size: 2109 1697 656 597 258 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2451 2832 2991 3020 1870 Size: 73 508 381 159 29 4545 Fragments arranged by size: 4545 508 381 159 73 29 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2738 2980 3016 4471 618 Size: 325 217 627 951 242 36 1455 1842 Fragments arranged by size: 1842 1455 951 627 325 242 217 36 BanII G_rGCy'C Cuts at: 1391 2267 2833 3014 1391 Size: 876 566 181 4072 Fragments arranged by size: 4072 876 566 181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 928 Size: 971 564 4160 Fragments arranged by size: 4160 971 564 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BfrBI ATG'CAT Cuts at: 2776 2776 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2706 4306 136 Size: 107 122 71 631 605 1034 1600 1525 Fragments arranged by size: 1600 1525 1034 631 605 122 107 71 BglII A'GATC_T Cuts at: 2430 5690 2430 Size: 3260 2435 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2145 2833 3756 4912 1790 Size: 355 688 923 1156 2573 Fragments arranged by size: 2573 1156 923 688 355 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2789 3426 4384 Size: 416 53 83 399 24 1372 637 958 Cuts at: 4384 442 Size: 1753 Fragments arranged by size: 1753 1372 958 637 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 2401 2789 2879 4355 1239 Size: 849 313 388 90 1476 2579 Fragments arranged by size: 2579 1476 849 388 313 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2977 3486 493 Size: 711 1773 509 2702 Fragments arranged by size: 2702 1773 711 509 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 2553 4128 4526 4800 4947 5358 2487 Size: 66 1575 398 274 147 411 2824 Fragments arranged by size: 2824 1575 411 398 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2718 1186 Size: 928 604 4163 Fragments arranged by size: 4163 928 604 BseYI C'CCAG_C Cuts at: 2629 2637 2872 2953 4898 5394 2629 Size: 8 235 81 1945 496 2930 Fragments arranged by size: 2930 1945 496 235 81 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 2927 1163 Size: 573 1083 108 3931 Fragments arranged by size: 3931 1083 573 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3145 3906 4934 1540 Size: 275 186 937 207 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 207 186 BsiHKAI G_wGCw'C Cuts at: 2267 3756 3841 4912 2267 Size: 1489 85 1071 3050 Fragments arranged by size: 3050 1489 1071 85 BsmI GAATG_Cn' Cuts at: 2767 3206 3299 2767 Size: 439 93 5163 Fragments arranged by size: 5163 439 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2267 2833 3014 3756 3841 4912 Size: 399 355 122 566 181 742 85 1071 Cuts at: 4912 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 566 399 355 181 122 85 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3050 844 Size: 2206 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2741 2933 4339 4526 1700 Size: 110 931 192 1406 187 2869 Fragments arranged by size: 2869 1406 931 192 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2465 1184 Size: 1281 4414 BstXI CCAn_nnnn'nTGG Cuts at: 2928 2977 2928 Size: 49 5646 BstYI r'GATC_y Cuts at: 1972 2430 3025 3392 3777 3794 5246 5332 5344 Size: 458 595 367 385 17 1452 86 12 Cuts at: 5344 5690 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 595 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2758 3211 4005 4025 2031 Size: 727 453 794 20 3701 Fragments arranged by size: 3701 794 727 453 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2084 2119 529 Size: 35 1520 35 4105 Fragments arranged by size: 4105 1520 35 35 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2935 3142 4031 Size: 54 1425 50 275 18 1105 207 889 Cuts at: 4031 5660 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1105 889 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 3142 1537 Size: 275 1123 207 4090 Fragments arranged by size: 4090 1123 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 2966 3625 1353 Size: 1613 659 3423 Fragments arranged by size: 3423 1613 659 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 4282 4656 4802 387 Size: 1064 138 540 2153 374 146 1280 Fragments arranged by size: 2153 1280 1064 540 374 146 138 EcoICRI GAG'CTC Cuts at: 2265 2265 Size: 5695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2088 2401 2789 2879 3758 4355 Size: 393 390 66 313 388 90 879 597 Cuts at: 4355 5142 5410 1239 Size: 787 268 1524 Fragments arranged by size: 1524 879 787 597 393 390 388 313 268 90 66 EcoO109I rG'GnC_Cy Cuts at: 2493 2854 2902 2493 Size: 361 48 5286 Fragments arranged by size: 5286 361 48 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 EcoRV GAT'ATC Cuts at: 2669 2669 Size: 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2636 2840 4842 1678 Size: 958 204 2002 2531 Fragments arranged by size: 2531 2002 958 204 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2520 2552 4336 Size: 32 135 32 905 32 11 32 1784 Cuts at: 4336 4368 4410 4442 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1784 905 135 42 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2544 2563 2940 4809 4993 1305 Size: 1239 19 377 1869 184 2007 Fragments arranged by size: 2007 1869 1239 377 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2514 2926 3653 4012 4171 518 Size: 648 1135 213 412 727 359 159 2042 Fragments arranged by size: 2042 1135 727 648 412 359 213 159 MspA1I CmG'CkG Cuts at: 1606 2644 2886 3111 3788 4936 5181 1606 Size: 1038 242 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 1038 677 245 242 225 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NsiI A_TGCA'T Cuts at: 2778 2778 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 2270 2497 1348 Size: 922 227 4546 Fragments arranged by size: 4546 922 227 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2861 2893 3764 3796 5277 5309 2861 Size: 32 871 32 1481 32 3247 Fragments arranged by size: 3247 1481 871 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2493 2902 2493 Size: 409 5286 PshAI GACnn'nnGTC Cuts at: 1613 2463 1613 Size: 850 4845 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2829 2829 Size: 5695 PstI C_TGCA'G Cuts at: 830 2592 2649 2676 3047 830 Size: 1762 57 27 371 3478 Fragments arranged by size: 3478 1762 371 57 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2644 2644 Size: 5695 SacI G_AGCT'C Cuts at: 2267 2267 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2398 2588 2645 2672 3043 4178 4859 Size: 219 1353 190 57 27 371 1135 681 Cuts at: 4859 5050 826 Size: 191 1471 Fragments arranged by size: 1471 1353 1135 681 371 219 191 190 57 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2834 3022 1872 Size: 962 188 4545 Fragments arranged by size: 4545 962 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4700 4962 820 Size: 197 926 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SrfI GCCC'GGGC Cuts at: 2834 2834 Size: 5695 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2190 1517 Size: 673 5022 StyI C'CwwG_G Cuts at: 513 1065 2004 3134 513 Size: 552 939 1130 3074 Fragments arranged by size: 3074 1130 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2998 3722 3739 3892 4077 4522 325 Size: 1255 1418 724 17 153 185 445 1498 Fragments arranged by size: 1498 1418 1255 724 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2832 3020 1870 Size: 962 188 4545 Fragments arranged by size: 4545 962 188 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI