(Circular) (Six-base) MAPSORT of: pFN21AB8641.seq Check: 2273 from: 1 to: 7282 pFN21AB8641 7282 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2686 4395 2686 Size: 1709 5573 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5092 278 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4625 1937 Size: 2688 4594 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2571 2898 4388 5345 6729 6997 1632 Size: 720 219 327 1490 957 1384 268 1917 Fragments arranged by size: 1917 1490 1384 957 720 327 268 219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 4302 6181 7158 1203 Size: 3099 1879 977 1327 Fragments arranged by size: 3099 1879 1327 977 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 3539 4026 1166 Size: 2373 487 4422 Fragments arranged by size: 4422 2373 487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3243 1184 Size: 2059 5223 AlwNI CAG_nnn'CTG Cuts at: 4274 4402 5025 6597 4274 Size: 128 623 1572 4959 Fragments arranged by size: 4959 1572 623 128 ApaLI G'TGCA_C Cuts at: 5339 6495 5339 Size: 1156 6126 ApoI r'AATT_y Cuts at: 1493 2054 2354 4591 4817 4969 7078 1493 Size: 561 300 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 561 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 3356 5836 160 Size: 872 2324 2480 1606 Fragments arranged by size: 2480 2324 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 3192 3948 4041 4607 1870 Size: 73 1249 756 93 566 4545 Fragments arranged by size: 4545 1249 756 566 93 73 BamHI G'GATC_C Cuts at: 4612 4979 4612 Size: 367 6915 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2521 2674 3402 4603 6058 Size: 325 217 627 734 153 728 1201 1455 Cuts at: 6058 618 Size: 1842 Fragments arranged by size: 1842 1455 1201 734 728 627 325 217 153 BanII G_rGCy'C Cuts at: 1391 3459 3822 4601 1391 Size: 2068 363 779 4072 Fragments arranged by size: 4072 2068 779 363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2543 3921 4305 4529 928 Size: 971 644 1378 384 224 3681 Fragments arranged by size: 3681 1378 971 644 384 224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5473 5507 1903 Size: 34 3536 34 3678 Fragments arranged by size: 3678 3536 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3771 4440 5174 6384 3771 Size: 669 734 1210 4669 Fragments arranged by size: 4669 1210 734 669 BclI T'GATC_A Cuts at: 1548 2560 3036 4092 1548 Size: 1012 476 1056 4738 Fragments arranged by size: 4738 1056 1012 476 BfrBI ATG'CAT Cuts at: 2894 2894 Size: 7282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5893 136 Size: 107 122 71 631 605 4221 1525 Fragments arranged by size: 4221 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7277 7277 Size: 7282 BlpI GC'TnA_GC Cuts at: 3453 4699 3453 Size: 1246 6036 Bme1580I G_kGCm'C Cuts at: 1790 2526 5343 6499 1790 Size: 736 2817 1156 2573 Fragments arranged by size: 2817 2573 1156 736 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 5013 5971 Size: 416 53 83 399 24 1511 2085 958 Cuts at: 5971 442 Size: 1753 Fragments arranged by size: 2085 1753 1511 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2185 2399 2847 2988 3321 3914 4301 5942 Size: 946 214 448 141 333 593 387 1641 Cuts at: 5942 1239 Size: 2579 Fragments arranged by size: 2579 1641 946 593 448 387 333 214 141 Bpu10I CC'TnA_GC Cuts at: 2373 3398 2373 Size: 1025 6257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4106 5407 6272 6570 4106 Size: 1301 865 298 4818 Fragments arranged by size: 4818 1301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2185 2825 3692 5945 882 Size: 617 686 640 867 2253 2219 Fragments arranged by size: 2253 2219 867 686 640 617 BsaAI yAC'GTr Cuts at: 493 1204 4001 5073 493 Size: 711 2797 1072 2702 Fragments arranged by size: 2797 2702 1072 711 BsaBI GATnn'nnATC Cuts at: 7276 7276 Size: 7282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5089 5471 6106 275 Size: 53 83 186 936 3556 382 635 1451 Fragments arranged by size: 3556 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2413 3426 5715 6113 6387 6534 6945 2413 Size: 1013 2289 398 274 147 411 2750 Fragments arranged by size: 2750 2289 1013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 2961 2991 3597 Size: 30 165 30 858 30 670 30 606 Cuts at: 3597 3627 3779 3809 1178 Size: 30 152 30 4651 Fragments arranged by size: 4651 858 670 606 165 152 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2276 2279 2282 2790 3089 3812 1186 Size: 1087 3 3 3 508 299 723 4656 Fragments arranged by size: 4656 1087 723 508 299 3 3 3 BseYI C'CCAG_C Cuts at: 2420 4395 6485 6981 2420 Size: 1975 2090 496 2721 Fragments arranged by size: 2721 2090 1975 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 4175 4229 1163 Size: 573 892 1547 54 4216 Fragments arranged by size: 4216 1547 892 573 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4732 5493 6521 1540 Size: 275 186 2731 761 1028 2301 Fragments arranged by size: 2731 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2510 2623 3459 5343 5428 6499 2510 Size: 113 836 1884 85 1071 3293 Fragments arranged by size: 3293 1884 1071 836 113 85 BsmI GAATG_Cn' Cuts at: 2794 4793 4886 2794 Size: 1999 93 5190 Fragments arranged by size: 5190 1999 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3680 3680 Size: 7282 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 2526 2623 3459 3822 4601 5343 Size: 399 720 16 97 836 363 779 742 Cuts at: 5343 5428 6499 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 836 779 742 720 399 363 97 85 16 BspEI T'CCGG_A Cuts at: 2413 6945 2413 Size: 4532 2750 BspHI T'CATG_A Cuts at: 2557 5171 6901 2557 Size: 2614 1730 2938 Fragments arranged by size: 2938 2614 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2686 4395 4637 844 Size: 1842 1709 242 3489 Fragments arranged by size: 3489 1842 1709 242 BsrBI CCG'CTC Cuts at: 1394 1823 5169 1394 Size: 429 3346 3507 Fragments arranged by size: 3507 3346 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2894 5777 5951 65 Size: 1183 1646 2883 174 1396 Fragments arranged by size: 2883 1646 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2608 3342 5926 6113 1700 Size: 110 798 734 2584 187 2869 Fragments arranged by size: 2869 2584 798 734 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7282 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 5342 6354 5342 Size: 1012 6270 BstAPI GCAn_nnn'nTGC Cuts at: 794 3301 4274 794 Size: 2507 973 3802 Fragments arranged by size: 3802 2507 973 BstBI TT'CG_AA Cuts at: 6086 6086 Size: 7282 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7282 BstXI CCAn_nnnn'nTGG Cuts at: 3589 4028 3589 Size: 439 6843 BstYI r'GATC_y Cuts at: 1972 2368 3705 4612 4979 5364 5381 6833 6919 Size: 396 1337 907 367 385 17 1452 86 Cuts at: 6919 6931 7277 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1337 907 396 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4083 4083 Size: 7282 BtgI C'CryG_G Cuts at: 513 1065 3678 513 Size: 552 2613 4117 Fragments arranged by size: 4117 2613 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6543 Fragments arranged by size: 6543 683 56 BtsI GCAGTG_nn' Cuts at: 2508 2780 2927 4798 5592 5612 2508 Size: 272 147 1871 794 20 4178 Fragments arranged by size: 4178 1871 794 272 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3921 3956 529 Size: 35 3357 35 3855 Fragments arranged by size: 3855 3357 35 35 DraI TTT'AAA Cuts at: 4586 4586 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6289 809 Size: 507 4973 1802 Fragments arranged by size: 4973 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3201 3234 4729 Size: 54 1425 50 275 18 1371 33 1495 Cuts at: 4729 5618 7247 8 Size: 889 1629 43 Fragments arranged by size: 1629 1495 1425 1371 889 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4729 1537 Size: 275 2917 4090 Fragments arranged by size: 4090 2917 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2250 2777 5212 1353 Size: 860 37 527 2435 3423 Fragments arranged by size: 3423 2435 860 527 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5869 6243 6389 387 Size: 1064 138 4280 374 146 1280 Fragments arranged by size: 4280 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3457 3457 Size: 7282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2352 2399 2571 2847 2898 2988 Size: 393 553 167 47 172 276 51 90 Cuts at: 2988 3321 3914 4301 4388 5345 5942 6729 6997 Size: 333 593 387 87 957 597 787 268 Cuts at: 6997 1239 Size: 1524 Fragments arranged by size: 1524 957 787 597 593 553 393 387 333 276 268 172 167 90 87 51 47 EcoO109I rG'GnC_Cy Cuts at: 2590 3023 3994 2590 Size: 433 971 5878 Fragments arranged by size: 5878 971 433 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 EcoRV GAT'ATC Cuts at: 3838 4138 3838 Size: 300 6982 FspI TGC'GCA Cuts at: 5788 5788 Size: 7282 HaeII r_GCGC'y Cuts at: 1678 3138 6429 1678 Size: 1460 3291 2531 Fragments arranged by size: 3291 2531 1460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2261 2293 2417 Size: 32 135 32 437 32 220 32 124 Cuts at: 2417 2449 2846 2878 2961 2993 3091 3123 3175 Size: 32 397 32 83 32 98 32 52 Cuts at: 3175 3207 3745 3777 5923 5955 5997 6029 1373 Size: 32 538 32 2146 32 42 32 2626 Fragments arranged by size: 2626 2146 538 437 397 220 135 124 98 83 52 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4626 4872 669 Size: 988 281 2688 246 3079 Fragments arranged by size: 3079 2688 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7282 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 4881 4881 Size: 7282 MluI A'CGCG_T Cuts at: 7158 7158 Size: 7282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 3102 4322 6396 6580 1305 Size: 1167 630 1220 2074 184 2007 Fragments arranged by size: 2074 2007 1220 1167 630 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3236 10 Size: 54 1425 343 1404 4056 Fragments arranged by size: 4056 1425 1404 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2753 3225 3539 4026 4172 5240 5599 Size: 648 1587 472 314 487 146 1068 359 Cuts at: 5599 5758 518 Size: 159 2042 Fragments arranged by size: 2042 1587 1068 648 487 472 359 314 159 146 MspA1I CmG'CkG Cuts at: 1606 3466 4274 4698 5375 6523 6768 1606 Size: 1860 808 424 677 1148 245 2120 Fragments arranged by size: 2120 1860 1148 808 677 424 245 NaeI GCC'GGC Cuts at: 3344 3344 Size: 7282 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6730 NdeI CA'TA_TG Cuts at: 387 387 Size: 7282 NgoMIV G'CCGG_C Cuts at: 3342 3342 Size: 7282 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7282 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NsiI A_TGCA'T Cuts at: 2896 2896 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 3860 3980 4306 4640 6185 1132 Size: 97 2631 120 326 334 1545 2229 Fragments arranged by size: 2631 2229 1545 334 326 120 97 PacI TTA_AT'TAA Cuts at: 3360 3360 Size: 7282 PasI CC'CwG_GG Cuts at: 1348 2386 1348 Size: 1038 6244 PciI A'CATG_T Cuts at: 4302 6181 4302 Size: 1879 5403 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5351 5383 6864 6896 5351 Size: 32 1481 32 5737 Fragments arranged by size: 5737 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2590 3994 2590 Size: 1404 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7282 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PspXI vC'TCGA_Gb Cuts at: 4041 4041 Size: 7282 PstI C_TGCA'G Cuts at: 830 4634 830 Size: 3804 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 PvuII CAG'CTG Cuts at: 3466 4274 3466 Size: 808 6474 SacI G_AGCT'C Cuts at: 3459 3459 Size: 7282 SalI G'TCGA_C Cuts at: 1936 4624 1936 Size: 2688 4594 SbfI CC_TGCA'GG Cuts at: 4634 4634 Size: 7282 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 2442 3990 2442 Size: 1548 5734 SfcI C'TryA_G Cuts at: 826 1045 4630 5765 6446 6637 826 Size: 219 3585 1135 681 191 1471 Fragments arranged by size: 3585 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7282 SmaI CCC'GGG Cuts at: 1872 4609 1872 Size: 2737 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3192 4041 4121 5386 6287 6549 Size: 197 926 1249 849 80 1265 901 262 Cuts at: 6549 820 Size: 1553 Fragments arranged by size: 1553 1265 1249 926 901 849 262 197 80 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 3860 4640 1132 Size: 2728 780 3774 Fragments arranged by size: 3774 2728 780 SspI AAT'ATT Cuts at: 5 52 5206 5 Size: 47 5154 2081 Fragments arranged by size: 5154 2081 47 StuI AGG'CCT Cuts at: 1517 4059 1517 Size: 2542 4740 StyI C'CwwG_G Cuts at: 513 1065 2517 2670 2704 3648 4721 513 Size: 552 1452 153 34 944 1073 3074 Fragments arranged by size: 3074 1452 1073 944 552 153 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 2662 5309 5326 5479 5664 6109 Size: 1255 659 423 2647 17 153 185 445 Cuts at: 6109 325 Size: 1498 Fragments arranged by size: 2647 1498 1255 659 445 423 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3741 4568 5528 Size: 274 80 33 51 1443 1764 827 960 Cuts at: 5528 7240 96 Size: 1712 138 Fragments arranged by size: 1764 1712 1443 960 827 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 4376 4408 7242 7274 2667 Size: 32 1677 32 2834 32 2675 Fragments arranged by size: 2834 2675 1677 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7282 XbaI T'CTAG_A Cuts at: 4618 4618 Size: 7282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3196 3345 3535 3196 Size: 149 190 6943 Fragments arranged by size: 6943 190 149 XhoI C'TCGA_G Cuts at: 1943 3192 4041 1943 Size: 1249 849 5184 Fragments arranged by size: 5184 1249 849 XmaI C'CCGG_G Cuts at: 1870 4607 1870 Size: 2737 4545 XmnI GAAnn'nnTTC Cuts at: 3731 5411 3731 Size: 1680 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5090 276 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NarI NruI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI