Restriction map for
pFN21AB8641
(Linear) (Six-base) MAPSORT of: KIBB8641.seq Check: 6849 from: 1 to: 2577
KIBB8641.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 681 2390 2577
Size: 681 1709 187
Fragments arranged by size:
1709 681 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 566 893 2383 2577
Size: 347 219 327 1490 194
Fragments arranged by size:
1490 347 327 219 194
AflIII A'CryG_T
Cuts at: 0 2297 2577
Size: 2297 280
AleI CACnn'nnGTG
Cuts at: 0 1534 2021 2577
Size: 1534 487 556
Fragments arranged by size:
1534 556 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1238 2577
Size: 1238 1339
AlwNI CAG_nnn'CTG
Cuts at: 0 2269 2397 2577
Size: 2269 128 180
Fragments arranged by size:
2269 180 128
ApoI r'AATT_y
Cuts at: 0 49 349 2577
Size: 49 300 2228
Fragments arranged by size:
2228 300 49
AseI AT'TA_AT
Cuts at: 0 1351 2577
Size: 1351 1226
AvaI C'yCGr_G
Cuts at: 0 1187 1943 2036 2577
Size: 1187 756 93 541
Fragments arranged by size:
1187 756 541 93
BanI G'GyrC_C
Cuts at: 0 516 669 1397 2577
Size: 516 153 728 1180
Fragments arranged by size:
1180 728 516 153
BanII G_rGCy'C
Cuts at: 0 1454 1817 2577
Size: 1454 363 760
Fragments arranged by size:
1454 760 363
BbsI GAAGACnn'nnnn_
Cuts at: 0 538 1916 2300 2524 2577
Size: 538 1378 384 224 53
Fragments arranged by size:
1378 538 384 224 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 1766 2435 2577
Size: 1766 669 142
Fragments arranged by size:
1766 669 142
BclI T'GATC_A
Cuts at: 0 555 1031 2087 2577
Size: 555 476 1056 490
Fragments arranged by size:
1056 555 490 476
BfrBI ATG'CAT
Cuts at: 0 889 2577
Size: 889 1688
BlpI GC'TnA_GC
Cuts at: 0 1448 2577
Size: 1448 1129
Bme1580I G_kGCm'C
Cuts at: 0 521 2577
Size: 521 2056
BmrI ACTGGGnnnn_n'
Cuts at: 0 923 2577
Size: 923 1654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 394 842 983 1316 1909 2296 2577
Size: 180 214 448 141 333 593 387 281
Fragments arranged by size:
593 448 387 333 281 214 180 141
Bpu10I CC'TnA_GC
Cuts at: 0 368 1393 2577
Size: 368 1025 1184
Fragments arranged by size:
1184 1025 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2101 2577
Size: 2101 476
BsaI GGTCTCn'nnnn_
Cuts at: 0 180 820 1687 2577
Size: 180 640 867 890
Fragments arranged by size:
890 867 640 180
BsaAI yAC'GTr
Cuts at: 0 1996 2577
Size: 1996 581
BsaWI w'CCGG_w
Cuts at: 0 408 1421 2577
Size: 408 1013 1156
Fragments arranged by size:
1156 1013 408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 956 986 1592 1622 1774 1804
Size: 256 30 670 30 606 30 152 30
Cuts at: 1804 2577
Size: 773
Fragments arranged by size:
773 670 606 256 152 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 271 274 277 785 1084 1807 2577
Size: 268 3 3 3 508 299 723 770
Fragments arranged by size:
770 723 508 299 268 3 3 3
BseYI C'CCAG_C
Cuts at: 0 415 2390 2577
Size: 415 1975 187
Fragments arranged by size:
1975 415 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 2170 2224 2577
Size: 623 1547 54 353
Fragments arranged by size:
1547 623 353 54
BsiHKAI G_wGCw'C
Cuts at: 0 505 618 1454 2577
Size: 505 113 836 1123
Fragments arranged by size:
1123 836 505 113
BsmI GAATG_Cn'
Cuts at: 0 789 2577
Size: 789 1788
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1675 2577
Size: 1675 902
Bsp1286I G_dGCh'C
Cuts at: 0 505 521 618 1454 1817 2577
Size: 505 16 97 836 363 760
Fragments arranged by size:
836 760 505 363 97 16
BspEI T'CCGG_A
Cuts at: 0 408 2577
Size: 408 2169
BspHI T'CATG_A
Cuts at: 0 552 2577
Size: 552 2025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 681 2390 2577
Size: 681 1709 187
Fragments arranged by size:
1709 681 187
BsrDI GCAATG_nn'
Cuts at: 0 889 2577
Size: 889 1688
BsrFI r'CCGG_y
Cuts at: 0 603 1337 2577
Size: 603 734 1240
Fragments arranged by size:
1240 734 603
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1296 2269 2577
Size: 1296 973 308
Fragments arranged by size:
1296 973 308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1584 2023 2577
Size: 1584 439 554
Fragments arranged by size:
1584 554 439
BstYI r'GATC_y
Cuts at: 0 363 1700 2577
Size: 363 1337 877
Fragments arranged by size:
1337 877 363
Bsu36I CC'TnA_GG
Cuts at: 0 2078 2577
Size: 2078 499
BtgI C'CryG_G
Cuts at: 0 1673 2577
Size: 1673 904
BtsI GCAGTG_nn'
Cuts at: 0 503 775 922 2577
Size: 503 272 147 1655
Fragments arranged by size:
1655 503 272 147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1916 1951 2577
Size: 1916 35 626
Fragments arranged by size:
1916 626 35
EaeI y'GGCC_r
Cuts at: 0 1196 1229 2577
Size: 1196 33 1348
Fragments arranged by size:
1348 1196 33
EarI CTCTTCn'nnn_
Cuts at: 0 208 245 772 2577
Size: 208 37 527 1805
Fragments arranged by size:
1805 527 208 37
EcoICRI GAG'CTC
Cuts at: 0 1452 2577
Size: 1452 1125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 347 394 566 842 893 983 1316
Size: 180 167 47 172 276 51 90 333
Cuts at: 1316 1909 2296 2383 2577
Size: 593 387 87 194
Fragments arranged by size:
593 387 333 276 194 180 172 167
90 87 51 47
EcoO109I rG'GnC_Cy
Cuts at: 0 585 1018 1989 2577
Size: 585 433 971 588
Fragments arranged by size:
971 588 585 433
EcoRV GAT'ATC
Cuts at: 0 1833 2133 2577
Size: 1833 300 444
Fragments arranged by size:
1833 444 300
HaeII r_GCGC'y
Cuts at: 0 1133 2577
Size: 1133 1444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 256 288 412 444 841 873
Size: 4 32 220 32 124 32 397 32
Cuts at: 873 956 988 1086 1118 1170 1202 1740 1772
Size: 83 32 98 32 52 32 538 32
Cuts at: 1772 2577
Size: 805
Fragments arranged by size:
805 538 397 220 124 98 83 52
32 32 32 32 32 32 32 32
4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1097 2317 2577
Size: 467 630 1220 260
Fragments arranged by size:
1220 630 467 260
MscI TGG'CCA
Cuts at: 0 1231 2577
Size: 1231 1346
MslI CAynn'nnrTG
Cuts at: 0 748 1220 1534 2021 2167 2577
Size: 748 472 314 487 146 410
Fragments arranged by size:
748 487 472 410 314 146
MspA1I CmG'CkG
Cuts at: 0 1461 2269 2577
Size: 1461 808 308
Fragments arranged by size:
1461 808 308
NaeI GCC'GGC
Cuts at: 0 1339 2577
Size: 1339 1238
NgoMIV G'CCGG_C
Cuts at: 0 1337 2577
Size: 1337 1240
NsiI A_TGCA'T
Cuts at: 0 891 2577
Size: 891 1686
NspI r_CATG'y
Cuts at: 0 1855 1975 2301 2577
Size: 1855 120 326 276
Fragments arranged by size:
1855 326 276 120
PacI TTA_AT'TAA
Cuts at: 0 1355 2577
Size: 1355 1222
PasI CC'CwG_GG
Cuts at: 0 381 2577
Size: 381 2196
PciI A'CATG_T
Cuts at: 0 2297 2577
Size: 2297 280
PpuMI rG'GwC_Cy
Cuts at: 0 585 1989 2577
Size: 585 1404 588
Fragments arranged by size:
1404 588 585
PspXI vC'TCGA_Gb
Cuts at: 0 2036 2577
Size: 2036 541
PvuII CAG'CTG
Cuts at: 0 1461 2269 2577
Size: 1461 808 308
Fragments arranged by size:
1461 808 308
SacI G_AGCT'C
Cuts at: 0 1454 2577
Size: 1454 1123
SexAI A'CCwGG_T
Cuts at: 0 437 1985 2577
Size: 437 1548 592
Fragments arranged by size:
1548 592 437
SmlI C'TyrA_G
Cuts at: 0 1187 2036 2116 2577
Size: 1187 849 80 461
Fragments arranged by size:
1187 849 461 80
SphI G_CATG'C
Cuts at: 0 1855 2577
Size: 1855 722
StuI AGG'CCT
Cuts at: 0 2054 2577
Size: 2054 523
StyI C'CwwG_G
Cuts at: 0 512 665 699 1643 2577
Size: 512 153 34 944 934
Fragments arranged by size:
944 934 512 153 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 657 2577
Size: 234 423 1920
Fragments arranged by size:
1920 423 234
TatI w'GTAC_w
Cuts at: 0 1736 2563 2577
Size: 1736 827 14
Fragments arranged by size:
1736 827 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 2371 2403 2577
Size: 662 32 1677 32 174
Fragments arranged by size:
1677 662 174 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1191 1340 1530 2577
Size: 1191 149 190 1047
Fragments arranged by size:
1191 1047 190 149
XhoI C'TCGA_G
Cuts at: 0 1187 2036 2577
Size: 1187 849 541
Fragments arranged by size:
1187 849 541
XmnI GAAnn'nnTTC
Cuts at: 0 1726 2577
Size: 1726 851
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbsI BciVI BclI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV NsiI NspI PacI PasI PciI PpuMI
PspXI PvuII SacI SexAI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BglI BglII BmgBI BmtI BplI
BsaBI BsaHI BsiEI BsiWI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoNI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XmaI ZraI