Restriction map for
pFN21AB8638
(Linear) (Six-base) MAPSORT of: KIBB8638.seq Check: 2973 from: 1 to: 1122
KIBB8638.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1055 1122
Size: 764 291 67
Fragments arranged by size:
764 291 67
AfeI AGC'GCT
Cuts at: 0 850 1122
Size: 850 272
AflIII A'CryG_T
Cuts at: 0 101 1122
Size: 101 1021
AlwNI CAG_nnn'CTG
Cuts at: 0 829 1122
Size: 829 293
ApoI r'AATT_y
Cuts at: 0 91 301 307 403 585 628 911 1122
Size: 91 210 6 96 182 43 283 211
Fragments arranged by size:
283 211 210 182 96 91 43 6
BbvCI CC'TCA_GC
Cuts at: 0 1049 1122
Size: 1049 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 20 54 1122
Size: 20 34 1068
Fragments arranged by size:
1068 34 20
Bme1580I G_kGCm'C
Cuts at: 0 1084 1122
Size: 1084 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 1122
Size: 399 723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1122
Size: 865 257
Bpu10I CC'TnA_GC
Cuts at: 0 1049 1122
Size: 1049 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 994 1122
Size: 994 128
BsaWI w'CCGG_w
Cuts at: 0 666 1122
Size: 666 456
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 1122
Size: 715 407
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1122
Size: 959 163
BsmI GAATG_Cn'
Cuts at: 0 327 1122
Size: 327 795
Bsp1286I G_dGCh'C
Cuts at: 0 1084 1122
Size: 1084 38
BsrBI CCG'CTC
Cuts at: 0 1061 1122
Size: 1061 61
BsrFI r'CCGG_y
Cuts at: 0 551 619 1122
Size: 551 68 503
Fragments arranged by size:
551 503 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 531 1122
Size: 348 183 591
Fragments arranged by size:
591 348 183
BtgI C'CryG_G
Cuts at: 0 893 1122
Size: 893 229
BtsI GCAGTG_nn'
Cuts at: 0 968 1122
Size: 968 154
DraIII CAC_nnn'GTG
Cuts at: 0 1044 1122
Size: 1044 78
EaeI y'GGCC_r
Cuts at: 0 513 621 923 1122
Size: 513 108 302 199
Fragments arranged by size:
513 302 199 108
EarI CTCTTCn'nnn_
Cuts at: 0 475 1122
Size: 475 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 865 1055 1122
Size: 764 101 190 67
Fragments arranged by size:
764 190 101 67
EcoO109I rG'GnC_Cy
Cuts at: 0 1093 1122
Size: 1093 29
EcoRI G'AATT_C
Cuts at: 0 585 628 1122
Size: 585 43 494
Fragments arranged by size:
585 494 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 1122
Size: 666 456
HaeII r_GCGC'y
Cuts at: 0 852 1122
Size: 852 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 710 742 1122
Size: 235 32 443 32 380
Fragments arranged by size:
443 380 235 32 32
HincII GTy'rAC
Cuts at: 0 358 504 1122
Size: 358 146 618
Fragments arranged by size:
618 358 146
HindIII A'AGCT_T
Cuts at: 0 460 1122
Size: 460 662
MscI TGG'CCA
Cuts at: 0 515 1122
Size: 515 607
MslI CAynn'nnrTG
Cuts at: 0 529 1122
Size: 529 593
MspA1I CmG'CkG
Cuts at: 0 275 826 1122
Size: 275 551 296
Fragments arranged by size:
551 296 275
NaeI GCC'GGC
Cuts at: 0 553 621 1122
Size: 553 68 501
Fragments arranged by size:
553 501 68
NgoMIV G'CCGG_C
Cuts at: 0 551 619 1122
Size: 551 68 503
Fragments arranged by size:
551 503 68
NspI r_CATG'y
Cuts at: 0 105 1122
Size: 105 1017
PciI A'CATG_T
Cuts at: 0 101 1122
Size: 101 1021
PflMI CCAn_nnn'nTGG
Cuts at: 0 1044 1122
Size: 1044 78
PpuMI rG'GwC_Cy
Cuts at: 0 1093 1122
Size: 1093 29
PstI C_TGCA'G
Cuts at: 0 883 909 1122
Size: 883 26 213
Fragments arranged by size:
883 213 26
PvuII CAG'CTG
Cuts at: 0 275 826 1122
Size: 275 551 296
Fragments arranged by size:
551 296 275
SanDI GG'GwC_CC
Cuts at: 0 1093 1122
Size: 1093 29
SfcI C'TryA_G
Cuts at: 0 879 905 1122
Size: 879 26 217
Fragments arranged by size:
879 217 26
StyI C'CwwG_G
Cuts at: 0 326 1122
Size: 326 796
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 998 1122
Size: 133 865 124
Fragments arranged by size:
865 133 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 1122
Size: 748 32 342
Fragments arranged by size:
748 342 32
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI BbvCI BcgI Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BseRI BsgI BsmI
Bsp1286I BsrBI BsrFI BstXI BtgI BtsI DraIII EaeI
EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MscI MslI MspA1I NaeI NgoMIV NspI PciI
PflMI PpuMI PstI PvuII SanDI SfcI StyI TaqII
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI