Restriction map for
pFN21AB8636
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 120 GCGG CCGC(-3/-1)
AlwI 122 GGATC GGATC(4/5)
AlwNI 394 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 511 GAGCTC G[AT]GC[AT]^C
BanII 511 GAGCTC G[GA]GC[CT]^C
BbvI 684 GCTGC GCAGC(8/12)
BlpI 513 GCTCAGC GC^T[ACGT]AGC
BmyI 511 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 511 GAGCTC G[AT]GC[AT]^C
BsmAI 827 GAGAC GTCTC(1/5)
BsmFI 430 GTCCC GGGAC(10/14)
BsmI 659 GAATGC GAATGC(1/-1)
Bsp1286I 511 GAGCTC G[AGT]GC[ACT]^C
BstEII 165 GGTCACC G^GT[ACGT]ACC
BstF5I 999 GGATG GGATG(2/0)
BstPI 165 GGTCACC G^GT[ACGT]ACC
BstYI 122 GGATCT [GA]^GATC[CT]
CelII 513 GCTCAGC GC^T[ACGT]AGC
EcoO65I 165 GGTCACC G^GT[ACGT]ACC
EcoRI 298 GAATTC G^AATTC
EcoT14I 434 CCAAGG C^C[AT][AT]GG
EcoT22I 786 ATGCAT ATGCA^T
Fnu4HI 684 GCTGC GC^[ACGT]GC
FokI 999 GGATG GGATG(9/13)
HaeIII 904 GGCC GG^CC
HindIII 141 AAGCTT A^AGCTT
HpaI 481 GTTAAC GTT^AAC
ItaI 684 GCTGC GC^[ACGT]GC
MaeII 676 ACGT A^CGT
MflI 122 GGATCT [GA]^GATC[CT]
MwoI 28 GCTGTCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 786 ATGCAT ATGCA^T
PflMI 367 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 511 GAGCTC GAGCT^C
SapI 944 GAAGAGC GCTCTTC(1/4)
SmlI 601 CTTGAG C^T[CT][GA]AG
StyI 434 CCAAGG C^C[AT][AT]GG
TaqI 342 TCGA T^CGA
TseI 684 GCTGC G^C[AT]GC
TthHB8I 342 TCGA T^CGA
Van91I 367 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 122 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 20 GTAC GT^AC
743 GTAC
Asp700I 397 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
634 GAACAACTTC
BbsI 79 GAAGAC GAAGAC(2/6)
216 GAAGAC
BpuAI 79 GAAGAC GAAGAC(2/6)
216 GAAGAC
BssKI 170 CCAGG ^CC[ACGT]GG
367 CCAGG
BstNI 170 CCAGG CC^[AT]GG
367 CCAGG
EcoRII 170 CCAGG ^CC[AT]GG
367 CCAGG
MaeIII 166 GTCAC ^GT[ACGT]AC
936 GTCAC
MseI 145 TTAA T^TAA
482 TTAA
MvaI 170 CCAGG CC^[AT]GG
367 CCAGG
NspI 590 ACATGC [GA]CATG^[CT]
703 ACATGC
PleI 185 GAGTC GAGTC(4/5)
934 GAGTC
RsaI 20 GTAC GT^AC
743 GTAC
ScrFI 170 CCAGG CC^[ACGT]GG
367 CCAGG
SfcI 253 CTACAG C^T[GA][CT]AG
650 CTATAG
Tru9I 145 TTAA T^TAA
482 TTAA
Tsp45I 166 GTCAC ^GT[GC]AC
936 GTCAC
TspRI 836 CACTG CA[GC]TG[ACGT][ACGT]^
864 CAGTG
XmnI 397 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
634 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsaJI
BsiYI BslI BsrI Cac8I DdeI
DpnII EarI HincII HindII HinfI
HphI Ksp632I MaeI MboI MboII
MnlI NdeII NlaIII Sau3AI TfiI
Tsp509I