Restriction map for
pFN21AB8634
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsaI BseRI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BstAPI BstEII BstPI BstXI Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TfiI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 351 GTATAC GT^[AC][GT]AC
AccIII 44 TCCGGA T^CCGGA
AcsI 166 GAATTC [GA]^AATT[CT]
AgeI 515 ACCGGT A^CCGGT
Aor51HI 965 AGCGCT AGC^GCT
ApoI 166 GAATTC [GA]^AATT[CT]
Asp700I 439 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 188 CTCGGG C^[CT]CG[GA]G
BseAI 44 TCCGGA T^CCGGA
BsgI 977 GTGCAG GTGCAG(16/14)
BsiEI 135 CGACCG CG[GA][CT]^CG
BsmAI 248 GTCTC GTCTC(1/5)
BsmI 51 GAATGC GAATGC(1/-1)
BsoBI 188 CTCGGG C^[CT]CG[GA]G
BspEI 44 TCCGGA T^CCGGA
BspMI 119 ACCTGC ACCTGC(4/8)
BssSI 15 CTCGTG CACGAG(-5/-1)
Bst1107I 351 GTATAC GTA^TAC
BstBI 456 TTCGAA TT^CGAA
BstZ17I 351 GTATAC GTA^TAC
CfoI 966 GCGC GCG^C
EarI 250 CTCTTC CTCTTC(1/4)
Eco47III 965 AGCGCT AGC^GCT
EcoNI 921 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 166 GAATTC G^AATTC
EcoRV 616 GATATC GAT^ATC
HaeII 965 AGCGCT [GA]GCGC^[CT]
HhaI 966 GCGC GCG^C
HinP1I 966 GCGC G^CGC
HincII 335 GTCAAC GT[CT]^[GA]AC
HindII 335 GTCAAC GT[CT]^[GA]AC
HindIII 901 AAGCTT A^AGCTT
Ksp632I 250 CTCTTC CTCTTC(1/4)
MaeII 227 ACGT A^CGT
MaeIII 571 GTCAC ^GT[ACGT]AC
MroI 44 TCCGGA T^CCGGA
MspA1I 547 CAGCTG C[AC]G^C[GT]G
MwoI 556 GCTGAATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 456 TTCGAA TT^CGAA
PflMI 671 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 515 ACCGGT A^CCGGT
PvuII 547 CAGCTG CAG^CTG
SfaNI 417 GATGC GCATC(5/9)
SfuI 456 TTCGAA TT^CGAA
SspI 201 AATATT AAT^ATT
TaqI 457 TCGA T^CGA
Tsp45I 571 GTCAC ^GT[GC]AC
TthHB8I 457 TCGA T^CGA
Van91I 671 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 566 CCATCGTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 439 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 100 CGCG CG^CG
957 CGCG
AfaI 582 GTAC GT^AC
936 GTAC
AlwNI 294 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
715 CAGATCCTG
BanII 874 GAGCCC G[GA]GC[CT]^C
893 GGGCTC
BfaI 316 CTAG C^TAG
754 CTAG
BlpI 544 GCTCAGC GC^T[ACGT]AGC
968 GCTCAGC
BmyI 874 GAGCCC G[AGT]GC[ACT]^C
893 GGGCTC
BpmI 269 CTCCAG CTGGAG(16/14)
67 CTGGAG
Bpu1102I 544 GCTCAGC GC^T[ACGT]AGC
968 GCTCAGC
BsaBI 436 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
612 GATAGATATC
BsaWI 44 TCCGGA [AT]^CCGG[AT]
515 ACCGGT
Bsp1286I 874 GAGCCC G[AGT]GC[ACT]^C
893 GGGCTC
BsrFI 77 ACCGGC [GA]^CCGG[CT]
515 ACCGGT
BstUI 100 CGCG CG^CG
957 CGCG
BstYI 716 AGATCC [GA]^GATC[CT]
952 AGATCC
Cac8I 193 GCCAGC GC[ACGT]^[ACGT]GC
663 GCTGGC
CelII 544 GCTCAGC GC^T[ACGT]AGC
968 GCTCAGC
Cfr10I 77 ACCGGC [GA]^CCGG[CT]
515 ACCGGT
DraII 401 GGGACCC [GA]G^G[ACGT]CC[CT]
941 AGGACCT
EaeI 79 CGGCCA [CT]^GGCC[GA]
626 CGGCCA
EcoO109I 401 GGGACCC [GA]G^G[ACGT]CC[CT]
941 AGGACCT
EcoT14I 671 CCAAGG C^C[AT][AT]GG
869 CCTTGG
HphI 447 TCACC GGTGA(8/7)
572 TCACC
MaeI 316 CTAG C^TAG
754 CTAG
MamI 436 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
612 GATAGATATC
MflI 716 AGATCC [GA]^GATC[CT]
952 AGATCC
MslI 573 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATCACGGTG
MvnI 100 CGCG CG^CG
957 CGCG
NlaIII 162 CATG CATG^
744 CATG
PpuMI 401 GGGACCC [GA]G^G[AT]CC[CT]
941 AGGACCT
PstI 121 CTGCAG CTGCA^G
778 CTGCAG
RsaI 582 GTAC GT^AC
936 GTAC
SfcI 121 CTGCAG C^T[GA][CT]AG
778 CTGCAG
SmlI 346 CTCAAG C^T[CT][GA]AG
904 CTTGAG
StyI 671 CCAAGG C^C[AT][AT]GG
869 CCTTGG
XhoII 716 AGATCC [GA]^GATC[CT]
952 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MseI MspI
MvaI NciI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI