(Circular) (Six-base) MAPSORT of: pFN21AB8634.seq Check: 3692 from: 1 to: 5728 pFN21AB8634 5728 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2357 3071 1937 Size: 420 714 4594 Fragments arranged by size: 4594 714 420 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 3791 5175 5443 1632 Size: 534 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1384 534 268 AfeI AGC'GCT Cuts at: 2972 2972 Size: 5728 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 4627 5604 1203 Size: 3424 977 1327 Fragments arranged by size: 3424 1327 977 AgeI A'CCGG_T Cuts at: 2520 4559 2520 Size: 2039 3689 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 2582 1166 Size: 1416 4312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2284 2316 2284 Size: 32 5696 AlwNI CAG_nnn'CTG Cuts at: 2304 2725 3471 5043 2304 Size: 421 746 1572 2989 Fragments arranged by size: 2989 1572 746 421 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 2171 3037 3263 3415 5524 1493 Size: 678 866 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 678 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2193 3053 1870 Size: 73 250 860 4545 Fragments arranged by size: 4545 860 250 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3049 4504 618 Size: 325 217 627 1262 1455 1842 Fragments arranged by size: 1842 1455 1262 627 325 217 BanII G_rGCy'C Cuts at: 1391 2883 2902 3047 1391 Size: 1492 19 145 4072 Fragments arranged by size: 4072 1492 145 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 2550 2974 3145 2550 Size: 424 171 5133 Fragments arranged by size: 5133 424 171 Bme1580I G_kGCm'C Cuts at: 1790 3789 4945 1790 Size: 1999 1156 2573 Fragments arranged by size: 2573 1999 1156 BmgBI CAC'GTC Cuts at: 1318 2233 1318 Size: 915 4813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3459 4417 442 Size: 416 53 83 399 24 2042 958 1753 Fragments arranged by size: 2042 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2935 2935 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2259 4388 1239 Size: 854 166 2129 2579 Fragments arranged by size: 2579 2129 854 166 Bpu10I CC'TnA_GC Cuts at: 2099 2864 2885 2099 Size: 765 21 4942 Fragments arranged by size: 4942 765 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 2930 3853 4718 5016 2336 Size: 594 923 865 298 3048 Fragments arranged by size: 3048 923 865 594 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 2445 2621 5722 2445 Size: 176 3101 2451 Fragments arranged by size: 3101 2451 176 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2049 2520 4161 4559 4833 4980 5391 2049 Size: 471 1641 398 274 147 411 2386 Fragments arranged by size: 2386 1641 471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5503 Fragments arranged by size: 5503 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5728 BseYI C'CCAG_C Cuts at: 3013 4931 5427 3013 Size: 1918 496 3314 Fragments arranged by size: 3314 1918 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3003 1163 Size: 573 1267 3888 Fragments arranged by size: 3888 1267 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2143 3178 3939 4967 1540 Size: 275 186 142 1035 761 1028 2301 Fragments arranged by size: 2301 1035 1028 761 275 186 142 BsiHKAI G_wGCw'C Cuts at: 3789 3874 4945 3789 Size: 85 1071 4572 Fragments arranged by size: 4572 1071 85 BsmI GAATG_Cn' Cuts at: 2062 3239 3332 2062 Size: 1177 93 4458 Fragments arranged by size: 4458 1177 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2883 2902 3047 3789 3874 4945 1391 Size: 399 1093 19 145 742 85 1071 2174 Fragments arranged by size: 2174 1093 1071 742 399 145 85 19 BspEI T'CCGG_A Cuts at: 2049 5391 2049 Size: 3342 2386 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 3083 844 Size: 1289 950 3489 Fragments arranged by size: 3489 1289 950 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 2520 4372 4559 1700 Size: 110 272 438 1852 187 2869 Fragments arranged by size: 2869 1852 438 272 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 2020 3788 4800 2020 Size: 1768 1012 2948 Fragments arranged by size: 2948 1768 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5728 BstBI TT'CG_AA Cuts at: 2462 4532 2462 Size: 2070 3658 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstYI r'GATC_y Cuts at: 1972 2721 2957 3058 3425 3810 3827 5279 5365 Size: 749 236 101 367 385 17 1452 86 Cuts at: 5365 5377 5723 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 749 385 367 346 236 101 86 17 12 BstZ17I GTA'TAC Cuts at: 2358 2358 Size: 5728 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2631 3175 Size: 54 1425 50 275 18 254 547 544 Cuts at: 3175 4064 5693 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 547 544 275 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 3658 1353 Size: 908 1397 3423 Fragments arranged by size: 3423 1397 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2166 2259 3791 4388 5175 5443 Size: 393 461 73 93 1532 597 787 268 Cuts at: 5443 1239 Size: 1524 Fragments arranged by size: 1532 1524 787 597 461 393 268 93 73 EcoNI CCTnn'n_nnAGG Cuts at: 2930 2930 Size: 5728 EcoO109I rG'GnC_Cy Cuts at: 2407 2947 2407 Size: 540 5188 EcoRI G'AATT_C Cuts at: 2171 3037 3415 2171 Size: 866 378 4484 Fragments arranged by size: 4484 866 378 EcoRV GAT'ATC Cuts at: 2623 2623 Size: 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5728 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 2974 4875 1678 Size: 1296 1901 2531 Fragments arranged by size: 2531 1901 1296 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4369 4401 4443 4475 1373 Size: 32 135 32 2797 32 42 32 2626 Fragments arranged by size: 2797 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2342 3072 3318 669 Size: 988 281 404 730 246 3079 Fragments arranged by size: 3079 988 730 404 281 246 HindIII A'AGCT_T Cuts at: 748 2906 748 Size: 2158 3570 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3906 Fragments arranged by size: 3906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 2738 3686 4045 4204 518 Size: 648 1416 156 948 359 159 2042 Fragments arranged by size: 2042 1416 948 648 359 159 156 MspA1I CmG'CkG Cuts at: 1606 2554 3144 3821 4969 5214 1606 Size: 948 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 590 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 3086 4631 1132 Size: 97 1857 1545 2229 Fragments arranged by size: 2229 1857 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 4627 4627 Size: 5728 PflMI CCAn_nnn'nTGG Cuts at: 1108 2682 1108 Size: 1574 4154 PfoI T'CCnGG_A Cuts at: 2275 2275 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2003 2035 3797 3829 5310 5342 2003 Size: 32 1762 32 1481 32 2389 Fragments arranged by size: 2389 1762 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 2947 2407 Size: 540 5188 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PstI C_TGCA'G Cuts at: 830 2130 2787 3080 830 Size: 1300 657 293 3478 Fragments arranged by size: 3478 1300 657 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2554 2554 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SanDI GG'GwC_CC Cuts at: 2407 2407 Size: 5728 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2126 2783 3076 4211 4892 5083 826 Size: 219 1081 657 293 1135 681 191 1471 Fragments arranged by size: 1471 1135 1081 681 657 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2909 3832 4733 4995 820 Size: 197 926 408 558 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 558 408 262 197 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 2208 3652 5 Size: 47 2156 1444 2081 Fragments arranged by size: 2156 2081 1444 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2004 2676 2874 3167 513 Size: 552 939 672 198 293 3074 Fragments arranged by size: 3074 939 672 552 293 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3755 3772 3925 4110 4555 325 Size: 1255 2175 17 153 185 445 1498 Fragments arranged by size: 2175 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2940 3974 5686 Size: 274 80 33 51 1443 963 1034 1712 Cuts at: 5686 96 Size: 138 Fragments arranged by size: 1712 1443 1034 963 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5688 5720 5688 Size: 32 5696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 2578 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 2448 3857 2448 Size: 1409 4319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI