(Circular) (Six-base) MAPSORT of: pFN21AB8633.seq Check: 5408 from: 1 to: 6211 pFN21AB8633 6211 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3178 3532 3178 Size: 354 5857 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 4274 5658 5926 1632 Size: 824 1818 1384 268 1917 Fragments arranged by size: 1917 1818 1384 824 268 AfeI AGC'GCT Cuts at: 2945 2945 Size: 6211 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 2157 5110 6087 1203 Size: 954 2953 977 1327 Fragments arranged by size: 2953 1327 977 954 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 4953 AlwNI CAG_nnn'CTG Cuts at: 3107 3954 5526 3107 Size: 847 1572 3792 Fragments arranged by size: 3792 1572 847 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 2546 3520 3746 3898 6007 1493 Size: 1053 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 1053 974 226 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3536 1870 Size: 73 1593 4545 Fragments arranged by size: 4545 1593 73 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2956 3178 3288 3532 Size: 325 217 627 224 945 222 110 244 Cuts at: 3532 4987 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 945 627 325 244 224 222 217 110 BanII G_rGCy'C Cuts at: 1391 2847 3038 3214 3530 1391 Size: 1456 191 176 316 4072 Fragments arranged by size: 4072 1456 316 191 176 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2642 2643 928 Size: 971 743 1 4496 Fragments arranged by size: 4496 971 743 1 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4402 4436 1903 Size: 34 2465 34 3678 Fragments arranged by size: 3678 2465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 2680 1548 Size: 1132 5079 BfrBI ATG'CAT Cuts at: 2891 2891 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4822 136 Size: 107 122 71 631 605 337 2813 1525 Fragments arranged by size: 2813 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 2430 3628 2430 Size: 1198 5013 Bme1580I G_kGCm'C Cuts at: 1790 3293 4272 5428 1790 Size: 1503 979 1156 2573 Fragments arranged by size: 2573 1503 1156 979 BmgBI CAC'GTC Cuts at: 1318 2160 2740 1318 Size: 842 580 4789 Fragments arranged by size: 4789 842 580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 3128 3942 Size: 416 53 83 399 24 750 961 814 Cuts at: 3942 4900 442 Size: 958 1753 Fragments arranged by size: 1753 961 958 814 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3114 1056 Size: 2058 4153 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3233 3233 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 3194 3211 4871 1239 Size: 1331 624 17 1660 2579 Fragments arranged by size: 2579 1660 1331 624 17 Bpu10I CC'TnA_GC Cuts at: 3096 3096 Size: 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 2948 4336 5201 5499 2333 Size: 615 1388 865 298 3045 Fragments arranged by size: 3045 1388 865 615 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 4018 4400 5035 Size: 53 83 186 936 479 2006 382 635 Cuts at: 5035 275 Size: 1451 Fragments arranged by size: 2006 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5986 Fragments arranged by size: 5986 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2958 3214 1186 Size: 1772 256 4183 Fragments arranged by size: 4183 1772 256 BseYI C'CCAG_C Cuts at: 2322 2769 3066 3361 5414 5910 2322 Size: 447 297 295 2053 496 2623 Fragments arranged by size: 2623 2053 496 447 297 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2082 1163 Size: 573 346 5292 Fragments arranged by size: 5292 573 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 3214 3506 4272 4357 5428 2357 Size: 857 292 766 85 1071 3140 Fragments arranged by size: 3140 1071 857 766 292 85 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 2847 3038 3214 3293 3506 3530 Size: 399 567 490 191 176 79 213 24 Cuts at: 3530 4272 4357 5428 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 490 399 213 191 176 85 79 24 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 4100 5830 4100 Size: 1730 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2267 3566 844 Size: 1423 1299 3489 Fragments arranged by size: 3489 1423 1299 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2831 4706 4880 65 Size: 1183 1583 1875 174 1396 Fragments arranged by size: 1875 1583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 3400 3400 Size: 6211 BstYI r'GATC_y Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206 Size: 1569 367 385 17 1452 86 12 346 Cuts at: 6206 1972 Size: 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2600 2600 Size: 6211 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4720 Fragments arranged by size: 4720 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 2742 3390 3494 3727 4521 4541 2742 Size: 648 104 233 794 20 4412 Fragments arranged by size: 4412 794 648 233 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2732 5218 809 Size: 507 1416 2486 1802 Fragments arranged by size: 2486 1802 1416 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2477 3658 4547 Size: 54 1425 50 275 18 647 1181 889 Cuts at: 4547 6176 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1181 889 647 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3079 4141 1353 Size: 774 952 1062 3423 Fragments arranged by size: 3423 1062 952 774 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3212 3212 Size: 6211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2570 3194 3211 4274 4871 5658 Size: 393 824 114 624 17 1063 597 787 Cuts at: 5658 5926 1239 Size: 268 1524 Fragments arranged by size: 1524 1063 824 787 624 597 393 268 114 17 EcoO109I rG'GnC_Cy Cuts at: 2085 2085 Size: 6211 EcoRI G'AATT_C Cuts at: 2546 3520 3898 2546 Size: 974 378 4859 Fragments arranged by size: 4859 974 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2693 2461 Size: 232 5979 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 2015 2947 3360 5358 1678 Size: 337 932 413 1998 2531 Fragments arranged by size: 2531 1998 932 413 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 3025 3057 3167 Size: 32 135 32 902 32 519 32 110 Cuts at: 3167 3199 3254 3286 4852 4884 4926 4958 1373 Size: 32 55 32 1566 32 42 32 2626 Fragments arranged by size: 2626 1566 902 519 135 110 55 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3088 3555 3801 669 Size: 988 281 1150 467 246 3079 Fragments arranged by size: 3079 1150 988 467 281 246 HindIII A'AGCT_T Cuts at: 748 2367 3253 748 Size: 1619 886 3706 Fragments arranged by size: 3706 1619 886 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6211 KpnI G_GTAC'C Cuts at: 3182 3536 3182 Size: 354 5857 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5325 5509 1305 Size: 4020 184 2007 Fragments arranged by size: 4020 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2479 10 Size: 54 1425 343 647 3742 Fragments arranged by size: 3742 1425 647 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4169 4528 4687 518 Size: 648 3003 359 159 2042 Fragments arranged by size: 3003 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 3428 3627 4304 5452 5697 1606 Size: 501 1321 199 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 501 245 199 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6211 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4720 Fragments arranged by size: 4720 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 3110 1052 Size: 2058 4153 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NsiI A_TGCA'T Cuts at: 2893 2893 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 2820 3569 5114 1132 Size: 97 1591 749 1545 2229 Fragments arranged by size: 2229 1591 1545 749 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6211 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6211 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2085 2085 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PstI C_TGCA'G Cuts at: 830 3563 830 Size: 2733 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2107 3428 2107 Size: 1321 4890 SacI G_AGCT'C Cuts at: 3214 3214 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SanDI GG'GwC_CC Cuts at: 2085 2085 Size: 6211 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 3559 4694 5375 5566 826 Size: 219 2514 1135 681 191 1471 Fragments arranged by size: 2514 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 2927 4315 5216 5478 820 Size: 197 926 405 579 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 579 405 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 3480 4135 5 Size: 47 3428 655 2081 Fragments arranged by size: 3428 2081 655 47 StuI AGG'CCT Cuts at: 1517 2925 1517 Size: 1408 4803 StyI C'CwwG_G Cuts at: 513 1065 2004 3293 3650 513 Size: 552 939 1289 357 3074 Fragments arranged by size: 3074 1289 939 552 357 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 3286 4238 4255 4408 4593 5038 Size: 1255 934 772 952 17 153 185 445 Cuts at: 5038 325 Size: 1498 Fragments arranged by size: 1498 1255 952 934 772 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2450 4457 6169 Size: 274 80 33 51 1443 473 2007 1712 Cuts at: 6169 96 Size: 138 Fragments arranged by size: 2007 1712 1443 473 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3485 3517 6171 6203 3485 Size: 32 2654 32 3493 Fragments arranged by size: 3493 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2180 2881 2180 Size: 701 5510 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 3470 4340 3470 Size: 870 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI