Restriction map for
pFN21AB8633
(Linear) (Six-base) MAPSORT of: KIBB8633.seq Check: 6623 from: 1 to: 1506
KIBB8633.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
Acc65I G'GTAC_C
Cuts at: 0 1173 1506
Size: 1173 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1506
Size: 451 1055
AfeI AGC'GCT
Cuts at: 0 940 1506
Size: 940 566
AflIII A'CryG_T
Cuts at: 0 152 1506
Size: 152 1354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 1506
Size: 437 1069
AlwNI CAG_nnn'CTG
Cuts at: 0 1102 1506
Size: 1102 404
ApoI r'AATT_y
Cuts at: 0 541 1506
Size: 541 965
BanI G'GyrC_C
Cuts at: 0 6 951 1173 1283 1506
Size: 6 945 222 110 223
Fragments arranged by size:
945 223 222 110 6
BanII G_rGCy'C
Cuts at: 0 842 1033 1209 1506
Size: 842 191 176 297
Fragments arranged by size:
842 297 191 176
BbeI G_GCGC'C
Cuts at: 0 10 1506
Size: 10 1496
BbsI GAAGACnn'nnnn_
Cuts at: 0 637 638 1506
Size: 637 1 868
Fragments arranged by size:
868 637 1
BclI T'GATC_A
Cuts at: 0 675 1506
Size: 675 831
BfrBI ATG'CAT
Cuts at: 0 886 1506
Size: 886 620
BlpI GC'TnA_GC
Cuts at: 0 425 1506
Size: 425 1081
Bme1580I G_kGCm'C
Cuts at: 0 1288 1506
Size: 1288 218
BmgBI CAC'GTC
Cuts at: 0 155 735 1506
Size: 155 580 771
Fragments arranged by size:
771 580 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 1123 1506
Size: 162 961 383
Fragments arranged by size:
961 383 162
BmtI G_CTAG'C
Cuts at: 0 1109 1506
Size: 1109 397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1228 1506
Size: 1228 278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1189 1206 1506
Size: 565 624 17 300
Fragments arranged by size:
624 565 300 17
Bpu10I CC'TnA_GC
Cuts at: 0 1091 1506
Size: 1091 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 943 1506
Size: 328 615 563
Fragments arranged by size:
615 563 328
BsaHI Gr'CG_yC
Cuts at: 0 7 1506
Size: 7 1499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 953 1209 1506
Size: 953 256 297
Fragments arranged by size:
953 297 256
BseYI C'CCAG_C
Cuts at: 0 317 764 1061 1356 1506
Size: 317 447 297 295 150
Fragments arranged by size:
447 317 297 295 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1506
Size: 77 1429
BsiHKAI G_wGCw'C
Cuts at: 0 352 1209 1501 1506
Size: 352 857 292 5
Fragments arranged by size:
857 352 292 5
Bsp1286I G_dGCh'C
Cuts at: 0 352 842 1033 1209 1288 1501 1506
Size: 352 490 191 176 79 213 5
Fragments arranged by size:
490 352 213 191 176 79 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 262 1506
Size: 262 1244
BsrDI GCAATG_nn'
Cuts at: 0 826 1506
Size: 826 680
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 1506
Size: 1395 111
Bsu36I CC'TnA_GG
Cuts at: 0 595 1506
Size: 595 911
BtsI GCAGTG_nn'
Cuts at: 0 737 1385 1489 1506
Size: 737 648 104 17
Fragments arranged by size:
737 648 104 17
DrdI GACnn_nn'nnGTC
Cuts at: 0 727 1506
Size: 727 779
EaeI y'GGCC_r
Cuts at: 0 472 1506
Size: 472 1034
EarI CTCTTCn'nnn_
Cuts at: 0 122 1074 1506
Size: 122 952 432
Fragments arranged by size:
952 432 122
EcoICRI GAG'CTC
Cuts at: 0 1207 1506
Size: 1207 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 565 1189 1206 1506
Size: 451 114 624 17 300
Fragments arranged by size:
624 451 300 114 17
EcoO109I rG'GnC_Cy
Cuts at: 0 80 1506
Size: 80 1426
EcoRI G'AATT_C
Cuts at: 0 541 1506
Size: 541 965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 688 1506
Size: 456 232 818
Fragments arranged by size:
818 456 232
HaeII r_GCGC'y
Cuts at: 0 10 942 1355 1506
Size: 10 932 413 151
Fragments arranged by size:
932 413 151 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1020 1052 1162 1194 1249 1281
Size: 469 32 519 32 110 32 55 32
Cuts at: 1281 1506
Size: 225
Fragments arranged by size:
519 469 225 110 55 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1083 1506
Size: 1083 423
HindIII A'AGCT_T
Cuts at: 0 362 1248 1506
Size: 362 886 258
Fragments arranged by size:
886 362 258
KasI G'GCGC_C
Cuts at: 0 6 1506
Size: 6 1500
KpnI G_GTAC'C
Cuts at: 0 1177 1506
Size: 1177 329
MscI TGG'CCA
Cuts at: 0 474 1506
Size: 474 1032
MspA1I CmG'CkG
Cuts at: 0 102 1423 1506
Size: 102 1321 83
Fragments arranged by size:
1321 102 83
NarI GG'CG_CC
Cuts at: 0 7 1506
Size: 7 1499
NheI G'CTAG_C
Cuts at: 0 1105 1506
Size: 1105 401
NsiI A_TGCA'T
Cuts at: 0 888 1506
Size: 888 618
NspI r_CATG'y
Cuts at: 0 815 1506
Size: 815 691
PpuMI rG'GwC_Cy
Cuts at: 0 80 1506
Size: 80 1426
PvuII CAG'CTG
Cuts at: 0 102 1423 1506
Size: 102 1321 83
Fragments arranged by size:
1321 102 83
SacI G_AGCT'C
Cuts at: 0 1209 1506
Size: 1209 297
SanDI GG'GwC_CC
Cuts at: 0 80 1506
Size: 80 1426
SfoI GGC'GCC
Cuts at: 0 8 1506
Size: 8 1498
SmlI C'TyrA_G
Cuts at: 0 343 922 1506
Size: 343 579 584
Fragments arranged by size:
584 579 343
SspI AAT'ATT
Cuts at: 0 1475 1506
Size: 1475 31
StuI AGG'CCT
Cuts at: 0 920 1506
Size: 920 586
StyI C'CwwG_G
Cuts at: 0 1288 1506
Size: 1288 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 1281 1506
Size: 509 772 225
Fragments arranged by size:
772 509 225
TatI w'GTAC_w
Cuts at: 0 445 1506
Size: 445 1061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1480 1506
Size: 1480 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 876 1506
Size: 175 701 630
Fragments arranged by size:
701 630 175
XmnI GAAnn'nnTTC
Cuts at: 0 1465 1506
Size: 1465 41
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlfI AlwNI ApoI BanI
BanII BbeI BbsI BclI BfrBI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstXI Bsu36I
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI KpnI MscI
MspA1I NarI NheI NsiI NspI PpuMI PvuII SacI
SanDI SfoI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BglI BglII BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoNI EcoRV FseI FspI
FspAI HpaI MfeI MluI MmeI MslI NaeI NcoI
NdeI NgoMIV NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI
ZraI