Restriction map for
pFN21AB8632
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FseI FspI Hin1I
HindIII HpaI HphI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1263 CGCG CG^CG
AgeI 1682 ACCGGT A^CCGGT
AocI 2494 CCTGAGG CC^T[ACGT]AGG
AseI 1809 ATTAAT AT^TAAT
AsnI 1809 ATTAAT AT^TAAT
AvaI 1889 CCCGAG C^[CT]CG[GA]G
BanI 553 GGCACC G^G[CT][GA]CC
BbsI 699 GTCTTC GAAGAC(2/6)
BclI 1638 TGATCA T^GATCA
BlpI 2455 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2455 GCTAAGC GC^T[ACGT]AGC
BpuAI 699 GTCTTC GAAGAC(2/6)
BsaWI 1682 ACCGGT [AT]^CCGG[AT]
BsgI 1727 GTGCAG GTGCAG(16/14)
BsoBI 1889 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1403 TGTACA T^GTACA
BspHI 1839 TCATGA T^CATGA
BsrFI 1682 ACCGGT [GA]^CCGG[CT]
BsrGI 1403 TGTACA T^GTACA
BstUI 1263 CGCG CG^CG
BstXI 1583 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2494 CCTGAGG CC^T[ACGT]AGG
CelII 2455 GCTAAGC GC^T[ACGT]AGC
CfoI 320 GCGC GCG^C
Cfr10I 1682 ACCGGT [GA]^CCGG[CT]
DraIII 2063 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2494 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1448 ATGCAT ATGCA^T
FbaI 1638 TGATCA T^GATCA
HaeII 319 GGCGCT [GA]GCGC^[CT]
HgaI 697 GCGTC GACGC(5/10)
HhaI 320 GCGC GCG^C
HinP1I 320 GCGC G^CGC
HincII 1108 GTTGAC GT[CT]^[GA]AC
HindII 1108 GTTGAC GT[CT]^[GA]AC
MaeII 2210 ACGT A^CGT
MslI 2030 CATTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1263 CGCG CG^CG
NdeI 2034 CATATG CA^TATG
NsiI 1448 ATGCAT ATGCA^T
PflMI 234 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1682 ACCGGT A^CCGGT
PshAI 723 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1809 ATTAAT AT^TAAT
PstI 1749 CTGCAG CTGCA^G
RcaI 1839 TCATGA T^CATGA
SapI 1612 GAAGAGC GCTCTTC(1/4)
SpeI 522 ACTAGT A^CTAGT
SspBI 1403 TGTACA T^GTACA
Van91I 234 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1954 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1266 GTAC GT^AC
1404 GTAC
AlwNI 1232 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1730 CAGAGTCTG
ApaI 386 GGGCCC GGGCC^C
1094 GGGCCC
Asp700I 541 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1871 GAAGTGCTTC
AspI 159 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACGAGGTC
BamHI 1258 GGATCC G^GATCC
1827 GGATCC
BanII 386 GGGCCC G[GA]GC[CT]^C
1094 GGGCCC
BbvCI 1273 GCTGAGG CCTCAGC(-5/-2)
1435 GCTGAGG
BcnI 1692 CCGGG CC^[GC]GG
2162 CCGGG
BsrDI 202 GCAATG GCAATG(2/0)
1858 GCAATG
MwoI 1264 GCGTACTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1635 GCCTGATCAGC
NciI 1692 CCGGG CC^[GC]GG
2162 CCGGG
PflFI 159 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACGAGGTC
RsaI 1266 GTAC GT^AC
1404 GTAC
SexAI 760 ACCAGGT A^CC[AT]GGT
1497 ACCTGGT
SfcI 692 CTACAG C^T[GA][CT]AG
1749 CTGCAG
SspI 2286 AATATT AAT^ATT
2326 AATATT
StuI 650 AGGCCT AGG^CCT
1373 AGGCCT
TaqI 1701 TCGA T^CGA
1902 TCGA
Tsp45I 417 GTCAC ^GT[GC]AC
1480 GTCAC
Tth111I 159 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACGAGGTC
TthHB8I 1701 TCGA T^CGA
1902 TCGA
XmnI 541 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1871 GAAGTGCTTC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BalI BbvI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
NdeII NlaIII NlaIV PleI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI XhoII