(Circular) (Six-base) MAPSORT of: pFN21AB8632.seq Check: 5909 from: 1 to: 7219 pFN21AB8632 7219 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5029 278 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4562 1937 Size: 2625 4594 Acc65I G'GTAC_C Cuts at: 4540 4540 Size: 7219 AclI AA'CG_TT Cuts at: 1563 5346 5719 1563 Size: 3783 373 3063 Fragments arranged by size: 3783 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 3015 3198 3354 3620 3810 5282 6666 Size: 801 582 183 156 266 190 1472 1384 Cuts at: 6666 6934 1632 Size: 268 1917 Fragments arranged by size: 1917 1472 1384 801 582 268 266 190 183 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7022 AflIII A'CryG_T Cuts at: 1203 6118 7095 1203 Size: 4915 977 1327 Fragments arranged by size: 4915 1327 977 AgeI A'CCGG_T Cuts at: 3687 6050 3687 Size: 2363 4856 AhdI GACnn_n'nnGTC Cuts at: 5948 5948 Size: 7219 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7219 AlwNI CAG_nnn'CTG Cuts at: 3242 3740 4962 6534 3242 Size: 498 1222 1572 3927 Fragments arranged by size: 3927 1572 1222 498 ApaI G_GGCC'C Cuts at: 2395 3103 2395 Size: 708 6511 ApaLI G'TGCA_C Cuts at: 5276 6432 5276 Size: 1156 6063 ApoI r'AATT_y Cuts at: 1493 2402 3494 3549 3702 3803 3825 4234 4445 Size: 909 1092 55 153 101 22 409 211 Cuts at: 4445 4528 4754 4906 7015 1493 Size: 83 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 909 409 226 211 153 152 101 83 55 22 AseI AT'TA_AT Cuts at: 160 1032 3815 5773 160 Size: 872 2783 1958 1606 Fragments arranged by size: 2783 1958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 AvaI C'yCGr_G Cuts at: 1870 1943 3894 4544 1870 Size: 73 1951 650 4545 Fragments arranged by size: 4545 1951 650 73 BamHI G'GATC_C Cuts at: 3263 3832 4549 4916 3263 Size: 569 717 367 5566 Fragments arranged by size: 5566 717 569 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2558 4540 5995 618 Size: 325 217 627 771 1982 1455 1842 Fragments arranged by size: 1982 1842 1455 771 627 325 217 BanII G_rGCy'C Cuts at: 1391 2395 3103 4538 1391 Size: 1004 708 1435 4072 Fragments arranged by size: 4072 1435 1004 708 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2697 928 Size: 971 798 5450 Fragments arranged by size: 5450 971 798 BbvCI CC'TCA_GC Cuts at: 3279 3441 3279 Size: 162 7057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5410 5444 1903 Size: 34 3473 34 3678 Fragments arranged by size: 3678 3473 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5111 6321 5111 Size: 1210 6009 BclI T'GATC_A Cuts at: 1548 3643 1548 Size: 2095 5124 BfrBI ATG'CAT Cuts at: 3455 3455 Size: 7219 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5830 136 Size: 107 122 71 631 605 4158 1525 Fragments arranged by size: 4158 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7214 7214 Size: 7219 BlpI GC'TnA_GC Cuts at: 4461 4636 4461 Size: 175 7044 Bme1580I G_kGCm'C Cuts at: 1790 2035 2395 3103 5280 6436 1790 Size: 245 360 708 2177 1156 2573 Fragments arranged by size: 2573 2177 1156 708 360 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 2151 3283 Size: 416 53 83 399 24 602 132 1132 Cuts at: 3283 4950 5908 442 Size: 1667 958 1753 Fragments arranged by size: 1753 1667 1132 958 602 416 399 132 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 7219 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 2739 3051 3445 5879 1239 Size: 1140 360 312 394 2434 2579 Fragments arranged by size: 2579 2434 1140 394 360 312 Bpu10I CC'TnA_GC Cuts at: 2957 3279 3441 2957 Size: 322 162 6735 Fragments arranged by size: 6735 322 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2259 3596 4106 4439 5344 6209 6507 2259 Size: 1337 510 333 905 865 298 2971 Fragments arranged by size: 2971 1337 905 865 510 333 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 2684 4307 5882 882 Size: 617 657 528 1623 1575 2219 Fragments arranged by size: 2219 1623 1575 657 617 528 BsaAI yAC'GTr Cuts at: 493 1204 5010 493 Size: 711 3806 2702 Fragments arranged by size: 3806 2702 711 BsaBI GATnn'nnATC Cuts at: 7213 7213 Size: 7219 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5026 5408 6043 275 Size: 53 83 186 936 3493 382 635 1451 Fragments arranged by size: 3493 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3687 5652 6050 6324 6471 6882 3687 Size: 1965 398 274 147 411 4024 Fragments arranged by size: 4024 1965 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 1178 Size: 30 165 30 1461 30 5503 Fragments arranged by size: 5503 1461 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2618 2666 2948 3164 3260 3458 4466 1186 Size: 1432 48 282 216 96 198 1008 3939 Fragments arranged by size: 3939 1432 1008 282 216 198 96 48 BseYI C'CCAG_C Cuts at: 2057 2075 6422 6918 2057 Size: 18 4347 496 2358 Fragments arranged by size: 4347 2358 496 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3753 1163 Size: 573 2017 4629 Fragments arranged by size: 4629 2017 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4669 5430 6458 1540 Size: 275 186 2668 761 1028 2301 Fragments arranged by size: 2668 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5280 5365 6436 5280 Size: 85 1071 6063 Fragments arranged by size: 6063 1071 85 BsmI GAATG_Cn' Cuts at: 4730 4823 4730 Size: 93 7126 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2395 3103 4538 5280 5365 6436 Size: 399 245 360 708 1435 742 85 1071 Cuts at: 6436 1391 Size: 2174 Fragments arranged by size: 2174 1435 1071 742 708 399 360 245 85 BspEI T'CCGG_A Cuts at: 6882 6882 Size: 7219 BspHI T'CATG_A Cuts at: 3844 5108 6838 3844 Size: 1264 1730 4225 Fragments arranged by size: 4225 1730 1264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4574 844 Size: 3730 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5106 1394 Size: 429 3283 3507 Fragments arranged by size: 3507 3283 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2214 3870 5714 5888 65 Size: 1183 966 1656 1844 174 1396 Fragments arranged by size: 1844 1656 1396 1183 966 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3687 5863 6050 1700 Size: 110 1877 2176 187 2869 Fragments arranged by size: 2869 2176 1877 187 110 BsrGI T'GTAC_A Cuts at: 96 3408 96 Size: 3312 3907 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7219 BssSI C'ACGA_G Cuts at: 5279 6291 5279 Size: 1012 6207 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7219 BstBI TT'CG_AA Cuts at: 6023 6023 Size: 7219 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7219 BstXI CCAn_nnnn'nTGG Cuts at: 3595 3595 Size: 7219 BstYI r'GATC_y Cuts at: 1972 2816 2852 3073 3263 3832 4549 4916 5301 Size: 844 36 221 190 569 717 367 385 Cuts at: 5301 5318 6770 6856 6868 7214 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 844 717 569 385 367 346 221 190 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 4500 4500 Size: 7219 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6667 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6480 Fragments arranged by size: 6480 683 56 BtsI GCAGTG_nn' Cuts at: 2424 2472 4735 5529 5549 2424 Size: 48 2263 794 20 4094 Fragments arranged by size: 4094 2263 794 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7219 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4278 4313 529 Size: 35 3714 35 3435 Fragments arranged by size: 3714 3435 35 35 DraI TTT'AAA Cuts at: 4523 4523 Size: 7219 DraIII CAC_nnn'GTG Cuts at: 4073 4073 Size: 7219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6226 809 Size: 507 4910 1802 Fragments arranged by size: 4910 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2462 2469 3306 Size: 54 1425 50 275 18 632 7 837 Cuts at: 3306 4666 5555 7184 8 Size: 1360 889 1629 43 Fragments arranged by size: 1629 1425 1360 889 837 632 275 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 4666 1537 Size: 275 2854 4090 Fragments arranged by size: 4090 2854 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2718 2748 3030 3309 3333 3552 3612 Size: 705 660 30 282 279 24 219 60 Cuts at: 3612 3786 4311 5149 1353 Size: 174 525 838 3423 Fragments arranged by size: 3423 838 705 660 525 282 279 219 174 60 30 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3276 5806 6180 6326 387 Size: 1064 138 1687 2530 374 146 1280 Fragments arranged by size: 2530 1687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2433 2739 3015 3051 3198 3354 Size: 393 747 54 306 276 36 147 156 Cuts at: 3354 3445 3620 3810 5282 5879 6666 6934 1239 Size: 91 175 190 1472 597 787 268 1524 Fragments arranged by size: 1524 1472 787 747 597 393 306 276 268 190 175 156 147 91 54 36 EcoO109I rG'GnC_Cy Cuts at: 2127 2506 3099 3168 3354 3465 2127 Size: 379 593 69 186 111 5881 Fragments arranged by size: 5881 593 379 186 111 69 EcoRI G'AATT_C Cuts at: 3494 3549 3803 4528 4906 3494 Size: 55 254 725 378 5807 Fragments arranged by size: 5807 725 378 254 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3824 4411 3824 Size: 587 6632 FspI TGC'GCA Cuts at: 5725 5725 Size: 7219 HaeII r_GCGC'y Cuts at: 1678 2328 6366 1678 Size: 650 4038 2531 Fragments arranged by size: 4038 2531 650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2471 2503 2514 Size: 32 135 32 581 32 286 32 11 Cuts at: 2514 2546 2678 2710 2831 2863 3272 3304 3611 Size: 32 132 32 121 32 409 32 307 Cuts at: 3611 3643 3734 3766 5860 5892 5934 5966 1373 Size: 32 91 32 2094 32 42 32 2626 Fragments arranged by size: 2626 2094 581 409 307 286 135 132 121 91 42 32 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3115 4563 4809 669 Size: 988 281 1177 1448 246 3079 Fragments arranged by size: 3079 1448 1177 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7219 HpaI GTT'AAC Cuts at: 4809 4809 Size: 7219 KpnI G_GTAC'C Cuts at: 4544 4544 Size: 7219 MfeI C'AATT_G Cuts at: 4818 4818 Size: 7219 MluI A'CGCG_T Cuts at: 7095 7095 Size: 7219 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2872 6333 6517 1305 Size: 1567 3461 184 2007 Fragments arranged by size: 3461 2007 1567 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2464 2471 3308 10 Size: 54 1425 343 632 7 837 3921 Fragments arranged by size: 3921 1425 837 632 343 54 7 MslI CAynn'nnrTG Cuts at: 518 1166 4039 5177 5536 5695 518 Size: 648 2873 1138 359 159 2042 Fragments arranged by size: 2873 2042 1138 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3440 3759 4093 4635 5312 6460 6705 1606 Size: 1834 319 334 542 677 1148 245 2120 Fragments arranged by size: 2120 1834 1148 677 542 334 319 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6667 NdeI CA'TA_TG Cuts at: 387 4040 387 Size: 3653 3566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7219 NotI GC'GGCC_GC Cuts at: 4666 4666 Size: 7219 NsiI A_TGCA'T Cuts at: 3457 3457 Size: 7219 NspI r_CATG'y Cuts at: 1132 1229 4577 6122 1132 Size: 97 3348 1545 2229 Fragments arranged by size: 3348 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2563 2640 2748 1348 Size: 1215 77 108 5819 Fragments arranged by size: 5819 1215 108 77 PciI A'CATG_T Cuts at: 6118 6118 Size: 7219 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 6082 PfoI T'CCnGG_A Cuts at: 3696 3696 Size: 7219 PmeI GTTT'AAAC Cuts at: 4523 4523 Size: 7219 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2657 2689 5288 5320 6801 6833 2657 Size: 32 2599 32 1481 32 3043 Fragments arranged by size: 3043 2599 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2127 3168 3354 2127 Size: 1041 186 5992 Fragments arranged by size: 5992 1041 186 PshAI GACnn'nnGTC Cuts at: 1613 2732 1613 Size: 1119 6100 PsiI TTA'TAA Cuts at: 4376 4789 4376 Size: 413 6806 PspOMI G'GGCC_C Cuts at: 2391 3099 2391 Size: 708 6511 PstI C_TGCA'G Cuts at: 830 3758 4571 830 Size: 2928 813 3478 Fragments arranged by size: 3478 2928 813 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7219 PvuII CAG'CTG Cuts at: 3440 3759 4093 3440 Size: 319 334 6566 Fragments arranged by size: 6566 334 319 SalI G'TCGA_C Cuts at: 1936 4561 1936 Size: 2625 4594 SapI GCTCTTCn'nnn_ Cuts at: 3612 3612 Size: 7219 SbfI CC_TGCA'GG Cuts at: 4571 4571 Size: 7219 ScaI AGT'ACT Cuts at: 5467 7179 5467 Size: 1712 5507 SexAI A'CCwGG_T Cuts at: 2765 3502 2765 Size: 737 6482 SfcI C'TryA_G Cuts at: 826 1045 2697 3754 4567 5702 6383 6574 826 Size: 219 1652 1057 813 1135 681 191 1471 Fragments arranged by size: 1652 1471 1135 1057 813 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7219 SmaI CCC'GGG Cuts at: 1872 4546 1872 Size: 2674 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2274 3575 4085 4418 5323 6224 Size: 197 926 331 1301 510 333 905 901 Cuts at: 6224 6486 820 Size: 262 1553 Fragments arranged by size: 1553 1301 926 905 901 510 333 331 262 197 SnaBI TAC'GTA Cuts at: 493 5010 493 Size: 4517 2702 SpeI A'CTAG_T Cuts at: 152 2527 152 Size: 2375 4844 SphI G_CATG'C Cuts at: 1132 4577 1132 Size: 3445 3774 SspI AAT'ATT Cuts at: 5 52 4293 4333 5143 5 Size: 47 4241 40 810 2081 Fragments arranged by size: 4241 2081 810 47 40 StuI AGG'CCT Cuts at: 1517 2657 3380 1517 Size: 1140 723 5356 Fragments arranged by size: 5356 1140 723 StyI C'CwwG_G Cuts at: 513 1065 2118 2346 2370 4658 513 Size: 552 1053 228 24 2288 3074 Fragments arranged by size: 3074 2288 1053 552 228 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 2576 5246 5263 5416 5601 6046 Size: 1255 657 339 2670 17 153 185 445 Cuts at: 6046 325 Size: 1498 Fragments arranged by size: 2670 1498 1255 657 445 339 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3408 5465 7177 Size: 274 80 33 51 1443 1431 2057 1712 Cuts at: 7177 96 Size: 138 Fragments arranged by size: 2057 1712 1443 1431 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7179 7211 7179 Size: 32 7187 Tth111I GACn'n_nGTC Cuts at: 1316 2167 3482 1316 Size: 851 1315 5053 Fragments arranged by size: 5053 1315 851 XbaI T'CTAG_A Cuts at: 3959 4555 3959 Size: 596 6623 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2138 2210 2246 2402 2588 2883 2138 Size: 72 36 156 186 295 6474 Fragments arranged by size: 6474 295 186 156 72 36 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7219 XmaI C'CCGG_G Cuts at: 1870 4544 1870 Size: 2674 4545 XmnI GAAnn'nnTTC Cuts at: 2550 3880 5348 2550 Size: 1330 1468 4421 Fragments arranged by size: 4421 1468 1330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5027 276 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI