Restriction map for
pFN21AB8632
(Linear) (Six-base) MAPSORT of: KIBB8632.seq Check: 5295 from: 1 to: 2514
KIBB8632.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1010 1193 1349 1615 1805 2514
Size: 428 582 183 156 266 190 709
Fragments arranged by size:
709 582 428 266 190 183 156
AgeI A'CCGG_T
Cuts at: 0 1682 2514
Size: 1682 832
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 1735 2514
Size: 1237 498 779
Fragments arranged by size:
1237 779 498
ApaI G_GGCC'C
Cuts at: 0 390 1098 2514
Size: 390 708 1416
Fragments arranged by size:
1416 708 390
ApoI r'AATT_y
Cuts at: 0 397 1489 1544 1697 1798 1820 2229 2440
Size: 397 1092 55 153 101 22 409 211
Cuts at: 2440 2514
Size: 74
Fragments arranged by size:
1092 409 397 211 153 101 74 55
22
AseI AT'TA_AT
Cuts at: 0 1810 2514
Size: 1810 704
AvaI C'yCGr_G
Cuts at: 0 1889 2514
Size: 1889 625
BamHI G'GATC_C
Cuts at: 0 1258 1827 2514
Size: 1258 569 687
Fragments arranged by size:
1258 687 569
BanI G'GyrC_C
Cuts at: 0 553 2514
Size: 553 1961
BanII G_rGCy'C
Cuts at: 0 390 1098 2514
Size: 390 708 1416
Fragments arranged by size:
1416 708 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 692 2514
Size: 692 1822
BbvCI CC'TCA_GC
Cuts at: 0 1274 1436 2514
Size: 1274 162 1078
Fragments arranged by size:
1274 1078 162
BclI T'GATC_A
Cuts at: 0 1638 2514
Size: 1638 876
BfrBI ATG'CAT
Cuts at: 0 1450 2514
Size: 1450 1064
BlpI GC'TnA_GC
Cuts at: 0 2456 2514
Size: 2456 58
Bme1580I G_kGCm'C
Cuts at: 0 30 390 1098 2514
Size: 30 360 708 1416
Fragments arranged by size:
1416 708 360 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 146 1278 2514
Size: 14 132 1132 1236
Fragments arranged by size:
1236 1132 132 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 2514
Size: 669 1845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 734 1046 1440 2514
Size: 374 360 312 394 1074
Fragments arranged by size:
1074 394 374 360 312
Bpu10I CC'TnA_GC
Cuts at: 0 952 1274 1436 2514
Size: 952 322 162 1078
Fragments arranged by size:
1078 952 322 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1591 2101 2434 2514
Size: 254 1337 510 333 80
Fragments arranged by size:
1337 510 333 254 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 679 2302 2514
Size: 151 528 1623 212
Fragments arranged by size:
1623 528 212 151
BsaWI w'CCGG_w
Cuts at: 0 1682 2514
Size: 1682 832
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 2514
Size: 859 30 1625
Fragments arranged by size:
1625 859 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 661 943 1159 1255 1453 2461 2514
Size: 613 48 282 216 96 198 1008 53
Fragments arranged by size:
1008 613 282 216 198 96 53 48
BseYI C'CCAG_C
Cuts at: 0 52 70 2514
Size: 52 18 2444
Fragments arranged by size:
2444 52 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1748 2514
Size: 1748 766
Bsp1286I G_dGCh'C
Cuts at: 0 30 390 1098 2514
Size: 30 360 708 1416
Fragments arranged by size:
1416 708 360 30
BspHI T'CATG_A
Cuts at: 0 1839 2514
Size: 1839 675
BsrDI GCAATG_nn'
Cuts at: 0 209 1865 2514
Size: 209 1656 649
Fragments arranged by size:
1656 649 209
BsrFI r'CCGG_y
Cuts at: 0 1682 2514
Size: 1682 832
BsrGI T'GTAC_A
Cuts at: 0 1403 2514
Size: 1403 1111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1590 2514
Size: 1590 924
BstYI r'GATC_y
Cuts at: 0 811 847 1068 1258 1827 2514
Size: 811 36 221 190 569 687
Fragments arranged by size:
811 687 569 221 190 36
Bsu36I CC'TnA_GG
Cuts at: 0 2495 2514
Size: 2495 19
BtsI GCAGTG_nn'
Cuts at: 0 419 467 2514
Size: 419 48 2047
Fragments arranged by size:
2047 419 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2273 2308 2514
Size: 2273 35 206
Fragments arranged by size:
2273 206 35
DraIII CAC_nnn'GTG
Cuts at: 0 2068 2514
Size: 2068 446
EaeI y'GGCC_r
Cuts at: 0 457 464 1301 2514
Size: 457 7 837 1213
Fragments arranged by size:
1213 837 457 7
EarI CTCTTCn'nnn_
Cuts at: 0 53 713 743 1025 1304 1328 1547 1607
Size: 53 660 30 282 279 24 219 60
Cuts at: 1607 1781 2306 2514
Size: 174 525 208
Fragments arranged by size:
660 525 282 279 219 208 174 60
53 30 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1271 2514
Size: 1271 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 428 734 1010 1046 1193 1349 1440
Size: 374 54 306 276 36 147 156 91
Cuts at: 1440 1615 1805 2514
Size: 175 190 709
Fragments arranged by size:
709 374 306 276 190 175 156 147
91 54 36
EcoO109I rG'GnC_Cy
Cuts at: 0 122 501 1094 1163 1349 1460 2514
Size: 122 379 593 69 186 111 1054
Fragments arranged by size:
1054 593 379 186 122 111 69
EcoRI G'AATT_C
Cuts at: 0 1489 1544 1798 2514
Size: 1489 55 254 716
Fragments arranged by size:
1489 716 254 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1819 2406 2514
Size: 1819 587 108
Fragments arranged by size:
1819 587 108
HaeII r_GCGC'y
Cuts at: 0 323 2514
Size: 323 2191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 466 498 509 541 673 705
Size: 148 32 286 32 11 32 132 32
Cuts at: 705 826 858 1267 1299 1606 1638 1729 1761
Size: 121 32 409 32 307 32 91 32
Cuts at: 1761 2514
Size: 753
Fragments arranged by size:
753 409 307 286 148 132 121 91
32 32 32 32 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 1110 2514
Size: 1110 1404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 2514
Size: 867 1647
MscI TGG'CCA
Cuts at: 0 459 466 1303 2514
Size: 459 7 837 1211
Fragments arranged by size:
1211 837 459 7
MslI CAynn'nnrTG
Cuts at: 0 2034 2514
Size: 2034 480
MspA1I CmG'CkG
Cuts at: 0 1435 1754 2088 2514
Size: 1435 319 334 426
Fragments arranged by size:
1435 426 334 319
NdeI CA'TA_TG
Cuts at: 0 2035 2514
Size: 2035 479
NsiI A_TGCA'T
Cuts at: 0 1452 2514
Size: 1452 1062
PasI CC'CwG_GG
Cuts at: 0 558 635 743 2514
Size: 558 77 108 1771
Fragments arranged by size:
1771 558 108 77
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 2514
Size: 240 2274
PfoI T'CCnGG_A
Cuts at: 0 1691 2514
Size: 1691 823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 2514
Size: 652 32 1830
Fragments arranged by size:
1830 652 32
PpuMI rG'GwC_Cy
Cuts at: 0 122 1163 1349 2514
Size: 122 1041 186 1165
Fragments arranged by size:
1165 1041 186 122
PshAI GACnn'nnGTC
Cuts at: 0 727 2514
Size: 727 1787
PsiI TTA'TAA
Cuts at: 0 2371 2514
Size: 2371 143
PspOMI G'GGCC_C
Cuts at: 0 386 1094 2514
Size: 386 708 1420
Fragments arranged by size:
1420 708 386
PstI C_TGCA'G
Cuts at: 0 1753 2514
Size: 1753 761
PvuII CAG'CTG
Cuts at: 0 1435 1754 2088 2514
Size: 1435 319 334 426
Fragments arranged by size:
1435 426 334 319
SapI GCTCTTCn'nnn_
Cuts at: 0 1607 2514
Size: 1607 907
SexAI A'CCwGG_T
Cuts at: 0 760 1497 2514
Size: 760 737 1017
Fragments arranged by size:
1017 760 737
SfcI C'TryA_G
Cuts at: 0 692 1749 2514
Size: 692 1057 765
Fragments arranged by size:
1057 765 692
SmlI C'TyrA_G
Cuts at: 0 269 1570 2080 2413 2514
Size: 269 1301 510 333 101
Fragments arranged by size:
1301 510 333 269 101
SpeI A'CTAG_T
Cuts at: 0 522 2514
Size: 522 1992
SspI AAT'ATT
Cuts at: 0 2288 2328 2514
Size: 2288 40 186
Fragments arranged by size:
2288 186 40
StuI AGG'CCT
Cuts at: 0 652 1375 2514
Size: 652 723 1139
Fragments arranged by size:
1139 723 652
StyI C'CwwG_G
Cuts at: 0 113 341 365 2514
Size: 113 228 24 2149
Fragments arranged by size:
2149 228 113 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 571 2514
Size: 232 339 1943
Fragments arranged by size:
1943 339 232
TatI w'GTAC_w
Cuts at: 0 1403 2514
Size: 1403 1111
Tth111I GACn'n_nGTC
Cuts at: 0 162 1477 2514
Size: 162 1315 1037
Fragments arranged by size:
1315 1037 162
XbaI T'CTAG_A
Cuts at: 0 1954 2514
Size: 1954 560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 133 205 241 397 583 878 2514
Size: 133 72 36 156 186 295 1636
Fragments arranged by size:
1636 295 186 156 133 72 36
XmnI GAAnn'nnTTC
Cuts at: 0 545 1875 2514
Size: 545 1330 639
Fragments arranged by size:
1330 639 545
Enzymes that do cut:
AcuI AgeI AlwNI ApaI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI Bsp1286I BspHI BsrDI BsrFI BsrGI
BstXI BstYI Bsu36I BtsI CspCI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NdeI NsiI PasI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
SapI SexAI SfcI SmlI SpeI SspI StuI StyI
TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII
BaeI BbeI BcgI BciVI BglI BglII BmgBI BmtI
BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI ClaI DraI DrdI EagI EcoICRI
EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PciI PmeI PmlI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI TstI XhoI XmaI ZraI