(Circular) (Six-base) MAPSORT of: pFN21AB8628.seq Check: 3789 from: 1 to: 7228 pFN21AB8628 7228 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5038 278 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2773 2782 4571 1937 Size: 836 9 1789 4594 Fragments arranged by size: 4594 1789 836 9 Acc65I G'GTAC_C Cuts at: 4549 4549 Size: 7228 AclI AA'CG_TT Cuts at: 1563 5355 5728 1563 Size: 3792 373 3063 Fragments arranged by size: 3792 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2826 3956 4286 4415 5291 6675 6943 Size: 995 199 1130 330 129 876 1384 268 Cuts at: 6943 1632 Size: 1917 Fragments arranged by size: 1917 1384 1130 995 876 330 268 199 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7031 AflIII A'CryG_T Cuts at: 1203 6127 7104 1203 Size: 4924 977 1327 Fragments arranged by size: 4924 1327 977 AgeI A'CCGG_T Cuts at: 6059 6059 Size: 7228 AhdI GACnn_n'nnGTC Cuts at: 5957 5957 Size: 7228 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7228 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7228 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3355 3387 3355 Size: 32 7196 AlwNI CAG_nnn'CTG Cuts at: 4309 4971 6543 4309 Size: 662 1572 4994 Fragments arranged by size: 4994 1572 662 ApaLI G'TGCA_C Cuts at: 5285 6441 5285 Size: 1156 6072 ApoI r'AATT_y Cuts at: 1493 2379 2420 3693 4238 4537 4763 4915 7024 Size: 886 41 1273 545 299 226 152 2109 Cuts at: 7024 1493 Size: 1697 Fragments arranged by size: 2109 1697 1273 886 545 299 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4412 5782 160 Size: 872 3380 1370 1606 Fragments arranged by size: 3380 1606 1370 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 AvaI C'yCGr_G Cuts at: 1870 1943 3650 4553 1870 Size: 73 1707 903 4545 Fragments arranged by size: 4545 1707 903 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3590 3623 3590 Size: 33 7195 BamHI G'GATC_C Cuts at: 2836 4558 4925 2836 Size: 1722 367 5139 Fragments arranged by size: 5139 1722 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4549 6004 618 Size: 325 217 627 2762 1455 1842 Fragments arranged by size: 2762 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4058 4384 4547 1391 Size: 2667 326 163 4072 Fragments arranged by size: 4072 2667 326 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 928 Size: 971 620 5637 Fragments arranged by size: 5637 971 620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5419 5453 1903 Size: 34 3482 34 3678 Fragments arranged by size: 3678 3482 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2386 3591 5120 6330 2386 Size: 1205 1529 1210 3284 Fragments arranged by size: 3284 1529 1210 1205 BclI T'GATC_A Cuts at: 1548 1548 Size: 7228 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5839 136 Size: 107 122 71 631 605 4167 1525 Fragments arranged by size: 4167 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2569 3073 7223 2569 Size: 504 4150 2574 Fragments arranged by size: 4150 2574 504 BlpI GC'TnA_GC Cuts at: 4344 4645 4344 Size: 301 6927 Bme1580I G_kGCm'C Cuts at: 1790 5289 6445 1790 Size: 3499 1156 2573 Fragments arranged by size: 3499 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2907 4424 1318 Size: 1589 1517 4122 Fragments arranged by size: 4122 1589 1517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4319 4959 5917 Size: 416 53 83 399 24 2902 640 958 Cuts at: 5917 442 Size: 1753 Fragments arranged by size: 2902 1753 958 640 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7228 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2547 3102 2547 Size: 555 6673 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 4334 4398 4535 5888 1239 Size: 816 2279 64 137 1353 2579 Fragments arranged by size: 2579 2279 1353 816 137 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3566 5353 6218 6516 3566 Size: 1787 865 298 4278 Fragments arranged by size: 4278 1787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3073 3308 5891 882 Size: 617 1574 235 2583 2219 Fragments arranged by size: 2583 2219 1574 617 235 BsaAI yAC'GTr Cuts at: 493 1204 5019 493 Size: 711 3815 2702 Fragments arranged by size: 3815 2702 711 BsaBI GATnn'nnATC Cuts at: 7222 7222 Size: 7228 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5035 5417 6052 275 Size: 53 83 186 936 3502 382 635 1451 Fragments arranged by size: 3502 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5661 6059 6333 6480 6891 5661 Size: 398 274 147 411 5998 Fragments arranged by size: 5998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7003 Fragments arranged by size: 7003 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3075 3404 1186 Size: 1889 329 5010 Fragments arranged by size: 5010 1889 329 BseYI C'CCAG_C Cuts at: 6431 6927 6431 Size: 496 6732 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3625 1163 Size: 573 1889 4766 Fragments arranged by size: 4766 1889 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4678 5439 6467 1540 Size: 275 186 2677 761 1028 2301 Fragments arranged by size: 2677 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 5289 5374 6445 2079 Size: 3210 85 1071 2862 Fragments arranged by size: 3210 2862 1071 85 BsmI GAATG_Cn' Cuts at: 4739 4832 4739 Size: 93 7135 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 4058 4384 4547 5289 5374 6445 Size: 399 289 1979 326 163 742 85 1071 Cuts at: 6445 1391 Size: 2174 Fragments arranged by size: 2174 1979 1071 742 399 326 289 163 85 BspEI T'CCGG_A Cuts at: 6891 6891 Size: 7228 BspHI T'CATG_A Cuts at: 5117 6847 5117 Size: 1730 5498 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4583 844 Size: 3739 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5115 1394 Size: 429 3292 3507 Fragments arranged by size: 3507 3292 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2594 3599 4338 5723 5897 65 Size: 1183 1346 1005 739 1385 174 1396 Fragments arranged by size: 1396 1385 1346 1183 1005 739 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5872 6059 1700 Size: 110 4062 187 2869 Fragments arranged by size: 4062 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4269 96 Size: 4173 3055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7228 BssSI C'ACGA_G Cuts at: 5288 6300 5288 Size: 1012 6216 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7228 BstBI TT'CG_AA Cuts at: 6032 6032 Size: 7228 BstEII G'GTnAC_C Cuts at: 1184 3263 3754 1184 Size: 2079 491 4658 Fragments arranged by size: 4658 2079 491 BstYI r'GATC_y Cuts at: 1972 2569 2638 2836 3073 4558 4925 5310 5327 Size: 597 69 198 237 1485 367 385 17 Cuts at: 5327 6779 6865 6877 7223 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1485 1452 597 385 367 346 237 198 86 69 17 12 BstZ17I GTA'TAC Cuts at: 2774 2774 Size: 7228 Bsu36I CC'TnA_GG Cuts at: 4328 4328 Size: 7228 BtgI C'CryG_G Cuts at: 513 1065 2401 4059 513 Size: 552 1336 1658 3682 Fragments arranged by size: 3682 1658 1336 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6489 Fragments arranged by size: 6489 683 56 BtsI GCAGTG_nn' Cuts at: 4744 5538 5558 4744 Size: 794 20 6414 Fragments arranged by size: 6414 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7228 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7193 DraI TTT'AAA Cuts at: 2505 2764 3402 4532 2505 Size: 259 638 1130 5201 Fragments arranged by size: 5201 1130 638 259 DraIII CAC_nnn'GTG Cuts at: 4051 4051 Size: 7228 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6235 809 Size: 507 4919 1802 Fragments arranged by size: 4919 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2626 3988 4675 Size: 54 1425 50 275 18 796 1362 687 Cuts at: 4675 5564 7193 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1362 889 796 687 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4675 1537 Size: 275 2863 4090 Fragments arranged by size: 4090 2863 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4101 4110 4383 5158 1353 Size: 2748 9 273 775 3423 Fragments arranged by size: 3423 2748 775 273 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5815 6189 6335 387 Size: 1064 138 4226 374 146 1280 Fragments arranged by size: 4226 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2627 2826 3956 4286 4334 4398 Size: 393 423 572 199 1130 330 48 64 Cuts at: 4398 4415 4535 5291 5888 6675 6943 1239 Size: 17 120 756 597 787 268 1524 Fragments arranged by size: 1524 1130 787 756 597 572 423 393 330 268 199 120 64 48 17 EcoO109I rG'GnC_Cy Cuts at: 2981 2981 Size: 7228 EcoRI G'AATT_C Cuts at: 2420 4537 4915 2420 Size: 2117 378 4733 Fragments arranged by size: 4733 2117 378 FspI TGC'GCA Cuts at: 5734 5734 Size: 7228 HaeII r_GCGC'y Cuts at: 1678 6375 1678 Size: 4697 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4247 4279 5869 5901 5943 Size: 32 135 32 2675 32 1590 32 42 Cuts at: 5943 5975 1373 Size: 32 2626 Fragments arranged by size: 2675 2626 1590 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 4572 4818 669 Size: 988 281 957 1677 246 3079 Fragments arranged by size: 3079 1677 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 2212 3064 748 Size: 1464 852 4912 Fragments arranged by size: 4912 1464 852 HpaI GTT'AAC Cuts at: 4818 4818 Size: 7228 KpnI G_GTAC'C Cuts at: 4553 4553 Size: 7228 MfeI C'AATT_G Cuts at: 2110 2671 4827 2110 Size: 561 2156 4511 Fragments arranged by size: 4511 2156 561 MluI A'CGCG_T Cuts at: 7104 7104 Size: 7228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2230 2628 2682 3562 6342 6526 1305 Size: 925 398 54 880 2780 184 2007 Fragments arranged by size: 2780 2007 925 880 398 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 3990 10 Size: 54 1425 343 2158 3248 Fragments arranged by size: 3248 2158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 5186 5545 5704 518 Size: 648 1081 2939 359 159 2042 Fragments arranged by size: 2939 2042 1081 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4644 5321 6469 6714 1606 Size: 3038 677 1148 245 2120 Fragments arranged by size: 3038 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2401 4059 513 Size: 552 1336 1658 3682 Fragments arranged by size: 3682 1658 1336 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7228 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7228 NotI GC'GGCC_GC Cuts at: 4675 4675 Size: 7228 NspI r_CATG'y Cuts at: 1132 1229 2713 2893 3295 3985 4490 4586 6131 Size: 97 1484 180 402 690 505 96 1545 Cuts at: 6131 1132 Size: 2229 Fragments arranged by size: 2229 1545 1484 690 505 402 180 97 96 PasI CC'CwG_GG Cuts at: 1348 3558 1348 Size: 2210 5018 PciI A'CATG_T Cuts at: 6127 6127 Size: 7228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 4088 1108 Size: 1299 1681 4248 Fragments arranged by size: 4248 1681 1299 PfoI T'CCnGG_A Cuts at: 2977 4375 4512 2977 Size: 1398 137 5693 Fragments arranged by size: 5693 1398 137 PmeI GTTT'AAAC Cuts at: 4532 4532 Size: 7228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5297 5329 6810 6842 5297 Size: 32 1481 32 5683 Fragments arranged by size: 5683 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2981 2981 Size: 7228 PshAI GACnn'nnGTC Cuts at: 1613 3957 1613 Size: 2344 4884 PsiI TTA'TAA Cuts at: 4798 4798 Size: 7228 PstI C_TGCA'G Cuts at: 830 3591 3883 4580 830 Size: 2761 292 697 3478 Fragments arranged by size: 3478 2761 697 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7228 SalI G'TCGA_C Cuts at: 1936 4570 1936 Size: 2634 4594 SapI GCTCTTCn'nnn_ Cuts at: 4383 4383 Size: 7228 SbfI CC_TGCA'GG Cuts at: 4580 4580 Size: 7228 ScaI AGT'ACT Cuts at: 5476 7188 5476 Size: 1712 5516 SexAI A'CCwGG_T Cuts at: 2935 3576 2935 Size: 641 6587 SfcI C'TryA_G Cuts at: 826 1045 3587 3879 4072 4576 5711 6392 6583 Size: 219 2542 292 193 504 1135 681 191 Cuts at: 6583 826 Size: 1471 Fragments arranged by size: 2542 1471 1135 681 504 292 219 193 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7228 SmaI CCC'GGG Cuts at: 1872 3652 4555 1872 Size: 1780 903 4545 Fragments arranged by size: 4545 1780 903 SmlI C'TyrA_G Cuts at: 820 1017 1943 3545 5332 6233 6495 820 Size: 197 926 1602 1787 901 262 1553 Fragments arranged by size: 1787 1602 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5019 493 Size: 4526 2702 SpeI A'CTAG_T Cuts at: 152 3740 152 Size: 3588 3640 SphI G_CATG'C Cuts at: 1132 4586 1132 Size: 3454 3774 SspI AAT'ATT Cuts at: 5 52 2302 2634 3124 3161 3508 3837 5152 Size: 47 2250 332 490 37 347 329 1315 Cuts at: 5152 5 Size: 2081 Fragments arranged by size: 2250 2081 1315 490 347 332 329 47 37 StuI AGG'CCT Cuts at: 1517 1517 Size: 7228 StyI C'CwwG_G Cuts at: 513 1065 2287 2335 2401 3298 4059 4427 4667 Size: 552 1222 48 66 897 761 368 240 Cuts at: 4667 513 Size: 3074 Fragments arranged by size: 3074 1222 897 761 552 368 240 66 48 SwaI ATTT'AAAT Cuts at: 3402 3402 Size: 7228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5255 5272 5425 5610 6055 325 Size: 1255 3675 17 153 185 445 1498 Fragments arranged by size: 3675 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3975 4269 4416 Size: 274 80 33 51 1443 1998 294 147 Cuts at: 4416 5474 7186 96 Size: 1058 1712 138 Fragments arranged by size: 1998 1712 1443 1058 294 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2187 2219 2959 2991 7188 7220 2187 Size: 32 740 32 4197 32 2195 Fragments arranged by size: 4197 2195 740 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2905 1316 Size: 1589 5639 XbaI T'CTAG_A Cuts at: 2566 4564 2566 Size: 1998 5230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7228 XmaI C'CCGG_G Cuts at: 1870 3650 4553 1870 Size: 1780 903 4545 Fragments arranged by size: 4545 1780 903 XmnI GAAnn'nnTTC Cuts at: 3177 5357 3177 Size: 2180 5048 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5036 276 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI XcmI