Restriction map for
pFN21AB8627
(Circular) (Six-base) MAPSORT of: pFN21AB8627.seq Check: 7697 from: 1 to: 7027
pFN21AB8627 7027 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4837 278
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3643 4370 1937
Size: 1706 727 4594
Fragments arranged by size:
4594 1706 727
Acc65I G'GTAC_C
Cuts at: 4348 4348
Size: 7027
AclI AA'CG_TT
Cuts at: 1563 2600 5154 5527 1563
Size: 1037 2554 373 3063
Fragments arranged by size:
3063 2554 1037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2501 2778 3233 3412 3587 5090 6474
Size: 602 267 277 455 179 175 1503 1384
Cuts at: 6474 6742 1632
Size: 268 1917
Fragments arranged by size:
1917 1503 1384 602 455 277 268 267
179 175
AfeI AGC'GCT
Cuts at: 4306 4306
Size: 7027
AflII C'TTAA_G
Cuts at: 820 1017 3143 820
Size: 197 2126 4704
Fragments arranged by size:
4704 2126 197
AflIII A'CryG_T
Cuts at: 1203 5926 6903 1203
Size: 4723 977 1327
Fragments arranged by size:
4723 1327 977
AgeI A'CCGG_T
Cuts at: 5858 5858
Size: 7027
AhdI GACnn_n'nnGTC
Cuts at: 5756 5756
Size: 7027
AleI CACnn'nnGTG
Cuts at: 1166 3767 1166
Size: 2601 4426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3740 4056 1184
Size: 2556 316 4155
Fragments arranged by size:
4155 2556 316
AlwNI CAG_nnn'CTG
Cuts at: 3329 4132 4770 6342 3329
Size: 803 638 1572 4014
Fragments arranged by size:
4014 1572 803 638
ApaLI G'TGCA_C
Cuts at: 5084 6240 5084
Size: 1156 5871
ApoI r'AATT_y
Cuts at: 1493 2384 3941 4336 4562 4714 6823 1493
Size: 891 1557 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 1557 891 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2132 3315 4207 4352 1870
Size: 73 174 15 1183 892 145 4545
Fragments arranged by size:
4545 1183 892 174 145 73 15
BamHI G'GATC_C
Cuts at: 4357 4724 4357
Size: 367 6660
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 4032 4348 5803 618
Size: 325 217 627 472 1773 316 1455 1842
Fragments arranged by size:
1842 1773 1455 627 472 325 316 217
BanII G_rGCy'C
Cuts at: 1391 3565 3747 3861 4346 1391
Size: 2174 182 114 485 4072
Fragments arranged by size:
4072 2174 485 182 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2354 928
Size: 971 455 5601
Fragments arranged by size:
5601 971 455
BbvCI CC'TCA_GC
Cuts at: 2091 2091
Size: 7027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5218 5252 1903
Size: 34 3281 34 3678
Fragments arranged by size:
3678 3281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4919 6129 4919
Size: 1210 5817
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7027
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3674 3968 5638
Size: 107 122 71 631 605 2002 294 1670
Cuts at: 5638 136
Size: 1525
Fragments arranged by size:
2002 1670 1525 631 605 294 122 107
71
BglII A'GATC_T
Cuts at: 2458 3901 7022 2458
Size: 1443 3121 2463
Fragments arranged by size:
3121 2463 1443
BlpI GC'TnA_GC
Cuts at: 4444 4444
Size: 7027
Bme1580I G_kGCm'C
Cuts at: 1790 3964 4208 4214 5088 6244 1790
Size: 2174 244 6 874 1156 2573
Fragments arranged by size:
2573 2174 1156 874 244 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2852 3587 4758
Size: 416 53 83 399 24 1435 735 1171
Cuts at: 4758 5716 442
Size: 958 1753
Fragments arranged by size:
1753 1435 1171 958 735 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 3840 4189 5687 1239
Size: 1236 1365 349 1498 2579
Fragments arranged by size:
2579 1498 1365 1236 349
Bpu10I CC'TnA_GC
Cuts at: 2091 2091
Size: 7027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3413 5152 6017 6315 3017
Size: 396 1739 865 298 3729
Fragments arranged by size:
3729 1739 865 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5690 882
Size: 617 4191 2219
Fragments arranged by size:
4191 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4818 493
Size: 711 3614 2702
Fragments arranged by size:
3614 2702 711
BsaBI GATnn'nnATC
Cuts at: 2946 3497 7021 2946
Size: 551 3524 2952
Fragments arranged by size:
3524 2952 551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4834 5216 5851 275
Size: 53 83 186 936 3301 382 635 1451
Fragments arranged by size:
3301 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5460 5858 6132 6279 6690 5460
Size: 398 274 147 411 5797
Fragments arranged by size:
5797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6802
Fragments arranged by size:
6802 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 3491 1186
Size: 949 1356 4722
Fragments arranged by size:
4722 1356 949
BseYI C'CCAG_C
Cuts at: 2252 3530 3669 3963 6230 6726 2252
Size: 1278 139 294 2267 496 2553
Fragments arranged by size:
2553 2267 1278 496 294 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3746 1163
Size: 573 2010 4444
Fragments arranged by size:
4444 2010 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4477 5238 6266 1540
Size: 275 186 2476 761 1028 2301
Fragments arranged by size:
2476 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2959 3747 4250 5088 5173 6244 2959
Size: 788 503 838 85 1071 3742
Fragments arranged by size:
3742 1071 838 788 503 85
BsmI GAATG_Cn'
Cuts at: 2372 3281 4538 4631 2372
Size: 909 1257 93 4768
Fragments arranged by size:
4768 1257 909 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2959 3565 3747 3861 3964 4208 4214
Size: 399 1169 606 182 114 103 244 6
Cuts at: 4214 4250 4346 5088 5173 6244 1391
Size: 36 96 742 85 1071 2174
Fragments arranged by size:
2174 1169 1071 742 606 399 244 182
114 103 96 85 36 6
BspEI T'CCGG_A
Cuts at: 6690 6690
Size: 7027
BspHI T'CATG_A
Cuts at: 3091 4916 6646 3091
Size: 1825 1730 3472
Fragments arranged by size:
3472 1825 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4382 844
Size: 3538 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4914 1394
Size: 429 3091 3507
Fragments arranged by size:
3507 3091 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5522 5696 65
Size: 1183 4274 174 1396
Fragments arranged by size:
4274 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 5671 5858 1700
Size: 110 295 3566 187 2869
Fragments arranged by size:
3566 2869 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 2881 96
Size: 2785 4242
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 3767 5087 6099 3767
Size: 1320 1012 4695
Fragments arranged by size:
4695 1320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3549 4218 794
Size: 2755 669 3603
Fragments arranged by size:
3603 2755 669
BstBI TT'CG_AA
Cuts at: 2365 5831 2365
Size: 3466 3561
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 7027
BstYI r'GATC_y
Cuts at: 1972 2113 2458 2947 3901 4357 4724 5109 5126
Size: 141 345 489 954 456 367 385 17
Cuts at: 5126 6578 6664 6676 7022 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 954 489 456 385 367 346
345 141 86 17 12
BstZ17I GTA'TAC
Cuts at: 3644 3644
Size: 7027
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5536
Fragments arranged by size:
5536 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2111 487
Size: 683 56 885 5403
Fragments arranged by size:
5403 885 683 56
BtsI GCAGTG_nn'
Cuts at: 2900 3964 4543 5337 5357 2900
Size: 1064 579 794 20 4570
Fragments arranged by size:
4570 1064 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6992
DraI TTT'AAA
Cuts at: 3580 4331 3580
Size: 751 6276
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6034 809
Size: 507 4718 1802
Fragments arranged by size:
4718 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3675 4474 5363
Size: 54 1425 50 275 18 1845 799 889
Cuts at: 5363 6992 8
Size: 1629 43
Fragments arranged by size:
1845 1629 1425 889 799 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4474 1537
Size: 275 2662 4090
Fragments arranged by size:
4090 2662 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3563 4957 1353
Size: 2210 1394 3423
Fragments arranged by size:
3423 2210 1394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5614 5988 6134 387
Size: 1064 138 4025 374 146 1280
Fragments arranged by size:
4025 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3745 3745
Size: 7027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2475 2501 2778 3233 3412 3587
Size: 393 602 241 26 277 455 179 175
Cuts at: 3587 3840 4189 5090 5687 6474 6742 1239
Size: 253 349 901 597 787 268 1524
Fragments arranged by size:
1524 901 787 602 597 455 393 349
277 268 253 241 179 175 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3473 3473
Size: 7027
EcoO109I rG'GnC_Cy
Cuts at: 2065 2480 3970 2065
Size: 415 1490 5122
Fragments arranged by size:
5122 1490 415
EcoRI G'AATT_C
Cuts at: 4336 4714 4336
Size: 378 6649
EcoRV GAT'ATC
Cuts at: 3516 3516
Size: 7027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2921 3407 2921
Size: 486 6541
FspI TGC'GCA
Cuts at: 5533 5533
Size: 7027
HaeII r_GCGC'y
Cuts at: 1678 4168 4308 6174 1678
Size: 2490 140 1866 2531
Fragments arranged by size:
2531 2490 1866 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3015 3047 3396 3428 5668
Size: 32 135 32 1443 32 349 32 2240
Cuts at: 5668 5700 5742 5774 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2240 1443 349 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4371 4617 669
Size: 988 281 2433 246 3079
Fragments arranged by size:
3079 2433 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3299 3583 748
Size: 2551 284 4192
Fragments arranged by size:
4192 2551 284
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KpnI G_GTAC'C
Cuts at: 4352 4352
Size: 7027
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 6903 6903
Size: 7027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6141 6325 1305
Size: 4836 184 2007
Fragments arranged by size:
4836 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3677 10
Size: 54 1425 343 1845 3360
Fragments arranged by size:
3360 1845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2976 3722 3767 4038 4985 5344 5503
Size: 648 1810 746 45 271 947 359 159
Cuts at: 5503 518
Size: 2042
Fragments arranged by size:
2042 1810 947 746 648 359 271 159
45
MspA1I CmG'CkG
Cuts at: 1606 2090 2200 2256 3673 4443 5120 6268 6513
Size: 484 110 56 1417 770 677 1148 245
Cuts at: 6513 1606
Size: 2120
Fragments arranged by size:
2120 1417 1148 770 677 484 245 110
56
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5536
Fragments arranged by size:
5536 939 552
NdeI CA'TA_TG
Cuts at: 387 2977 387
Size: 2590 4437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7027
NotI GC'GGCC_GC
Cuts at: 4474 4474
Size: 7027
NspI r_CATG'y
Cuts at: 1132 1229 4019 4385 5930 1132
Size: 97 2790 366 1545 2229
Fragments arranged by size:
2790 2229 1545 366 97
PasI CC'CwG_GG
Cuts at: 1348 4082 1348
Size: 2734 4293
PciI A'CATG_T
Cuts at: 5926 5926
Size: 7027
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7027
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 6609 6641 5096
Size: 32 1481 32 5482
Fragments arranged by size:
5482 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2679 1613
Size: 1066 5961
PsiI TTA'TAA
Cuts at: 4597 4597
Size: 7027
PstI C_TGCA'G
Cuts at: 830 2313 4379 830
Size: 1483 2066 3478
Fragments arranged by size:
3478 2066 1483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 2090 2200 2256 3673 2090
Size: 110 56 1417 5444
Fragments arranged by size:
5444 1417 110 56
SacI G_AGCT'C
Cuts at: 3747 3747
Size: 7027
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 3246 5275 6987 3246
Size: 2029 1712 3286
Fragments arranged by size:
3286 2029 1712
SfcI C'TryA_G
Cuts at: 826 1045 2309 2667 4375 5510 6191 6382 826
Size: 219 1264 358 1708 1135 681 191 1471
Fragments arranged by size:
1708 1471 1264 1135 681 358 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 3317 4209 4354 1872
Size: 1445 892 145 4545
Fragments arranged by size:
4545 1445 892 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 3032 3143 3392 5131 6032
Size: 197 926 174 915 111 249 1739 901
Cuts at: 6032 6294 820
Size: 262 1553
Fragments arranged by size:
1739 1553 926 915 901 262 249 197
174 111
SnaBI TAC'GTA
Cuts at: 493 4818 493
Size: 4325 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7027
SphI G_CATG'C
Cuts at: 1132 4019 4385 1132
Size: 2887 366 3774
Fragments arranged by size:
3774 2887 366
SrfI GCCC'GGGC
Cuts at: 4209 4209
Size: 7027
SspI AAT'ATT
Cuts at: 5 52 4951 5
Size: 47 4899 2081
Fragments arranged by size:
4899 2081 47
StuI AGG'CCT
Cuts at: 1517 3455 3733 4121 4317 1517
Size: 1938 278 388 196 4227
Fragments arranged by size:
4227 1938 388 278 196
StyI C'CwwG_G
Cuts at: 513 1065 2004 2721 3687 3799 4466 513
Size: 552 939 717 966 112 667 3074
Fragments arranged by size:
3074 966 939 717 667 552 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3270 5054 5071 5224 5409 5854
Size: 1255 462 1228 1784 17 153 185 445
Cuts at: 5854 325
Size: 1498
Fragments arranged by size:
1784 1498 1255 1228 462 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2881 3244 5273
Size: 274 80 33 51 1443 904 363 2029
Cuts at: 5273 6985 96
Size: 1712 138
Fragments arranged by size:
2029 1712 1443 904 363 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 6987 7019 3056
Size: 32 3899 32 3064
Fragments arranged by size:
3899 3064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3087 1316
Size: 1771 5256
XbaI T'CTAG_A
Cuts at: 3178 4363 3178
Size: 1185 5842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2253 3052 3685 2253
Size: 799 633 5595
Fragments arranged by size:
5595 799 633
XhoI C'TCGA_G
Cuts at: 1943 2117 1943
Size: 174 6853
XmaI C'CCGG_G
Cuts at: 1870 3315 4207 4352 1870
Size: 1445 892 145 4545
Fragments arranged by size:
4545 1445 892 145
XmnI GAAnn'nnTTC
Cuts at: 3246 5156 3246
Size: 1910 5117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4835 276
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SwaI