(Circular) (Six-base) MAPSORT of: pFN21AB8625.seq Check: 2738 from: 1 to: 5698 pFN21AB8625 5698 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 2997 3825 4198 1563 Size: 1434 828 373 3063 Fragments arranged by size: 3063 1434 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2501 2898 2963 3761 5145 5413 1632 Size: 454 415 397 65 798 1384 268 1917 Fragments arranged by size: 1917 1384 798 454 415 397 268 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2015 2047 2015 Size: 32 5666 AlwNI CAG_nnn'CTG Cuts at: 2536 3441 5013 2536 Size: 905 1572 3221 Fragments arranged by size: 3221 1572 905 ApaI G_GGCC'C Cuts at: 2310 2310 Size: 5698 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 2821 2990 3007 3233 3385 5494 1493 Size: 1328 169 17 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 169 152 17 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 AvrII C'CTAG_G Cuts at: 2491 2491 Size: 5698 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2619 3019 4474 618 Size: 325 217 627 832 400 1455 1842 Fragments arranged by size: 1842 1455 832 627 400 325 217 BanII G_rGCy'C Cuts at: 1391 2230 2310 2637 2670 2699 3017 1391 Size: 839 80 327 33 29 318 4072 Fragments arranged by size: 4072 839 327 318 80 33 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2213 928 Size: 971 314 4413 Fragments arranged by size: 4413 971 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2428 3590 4800 2428 Size: 1162 1210 3326 Fragments arranged by size: 3326 1210 1162 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2276 4309 136 Size: 107 122 71 631 605 604 2033 1525 Fragments arranged by size: 2033 1525 631 605 604 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2058 3115 2058 Size: 1057 4641 Bme1580I G_kGCm'C Cuts at: 1790 2310 3759 4915 1790 Size: 520 1449 1156 2573 Fragments arranged by size: 2573 1449 1156 520 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2759 4358 1239 Size: 1520 1599 2579 Fragments arranged by size: 2579 1599 1520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 2621 3823 4688 4986 2384 Size: 237 1202 865 298 3096 Fragments arranged by size: 3096 1202 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 4361 882 Size: 617 792 2070 2219 Fragments arranged by size: 2219 2070 792 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 2961 5692 2961 Size: 2731 2967 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2965 4131 4529 4803 4950 5361 2965 Size: 1166 398 274 147 411 3302 Fragments arranged by size: 3302 1166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 2684 2714 1178 Size: 30 165 30 612 30 639 30 4162 Fragments arranged by size: 4162 639 612 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2039 2154 2465 2705 1186 Size: 850 3 115 311 240 4179 Fragments arranged by size: 4179 850 311 240 115 3 BseYI C'CCAG_C Cuts at: 2394 4901 5397 2394 Size: 2507 496 2695 Fragments arranged by size: 2695 2507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 1163 Size: 573 363 4762 Fragments arranged by size: 4762 573 363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2670 2699 3759 3844 4915 2670 Size: 29 1060 85 1071 3453 Fragments arranged by size: 3453 1071 1060 85 29 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2230 2310 2637 2670 2699 3017 3759 Size: 399 440 80 327 33 29 318 742 Cuts at: 3759 3844 4915 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 327 318 85 80 33 29 BspEI T'CCGG_A Cuts at: 2965 5361 2965 Size: 2396 3302 BspHI T'CATG_A Cuts at: 2386 3587 5317 2386 Size: 1201 1730 2767 Fragments arranged by size: 2767 1730 1201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3053 844 Size: 1673 536 3489 Fragments arranged by size: 3489 1673 536 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2245 2263 3585 1394 Size: 429 206 216 18 1322 3507 Fragments arranged by size: 3507 1322 429 216 206 18 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 2066 2698 3758 4770 2066 Size: 632 1060 1012 2994 Fragments arranged by size: 2994 1060 1012 632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2302 2302 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2898 513 Size: 552 939 48 846 3313 Fragments arranged by size: 3313 939 846 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2555 4705 809 Size: 507 1239 2150 1802 Fragments arranged by size: 2150 1802 1239 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2268 2277 Size: 54 1425 50 275 18 219 219 9 Cuts at: 2277 3145 4034 5663 8 Size: 868 889 1629 43 Fragments arranged by size: 1629 1425 889 868 275 219 219 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2328 3628 1353 Size: 863 112 1300 3423 Fragments arranged by size: 3423 1300 863 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2025 2273 4285 4659 4805 387 Size: 1064 138 436 248 2012 374 146 1280 Fragments arranged by size: 2012 1280 1064 436 374 248 146 138 EcoICRI GAG'CTC Cuts at: 2668 2697 2668 Size: 29 5669 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2501 2759 2898 2963 3761 4358 Size: 393 454 415 258 139 65 798 597 Cuts at: 4358 5145 5413 1239 Size: 787 268 1524 Fragments arranged by size: 1524 798 787 597 454 415 393 268 258 139 65 EcoO109I rG'GnC_Cy Cuts at: 2306 2307 2306 Size: 1 5697 EcoRI G'AATT_C Cuts at: 2821 3007 3385 2821 Size: 186 378 5134 Fragments arranged by size: 5134 378 186 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2719 2751 4339 4371 4413 Size: 32 135 32 1147 32 1588 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 1588 1147 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4284 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 4812 4996 1305 Size: 1215 2292 184 2007 Fragments arranged by size: 2292 2007 1215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2051 2279 10 Size: 54 1425 343 219 228 3429 Fragments arranged by size: 3429 1425 343 228 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 3656 4015 4174 518 Size: 648 1434 1056 359 159 2042 Fragments arranged by size: 2042 1434 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2523 2614 3114 3791 4939 5184 1606 Size: 591 326 91 500 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 591 500 326 245 91 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 2898 513 Size: 552 939 48 846 3313 Fragments arranged by size: 3313 939 846 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2044 3056 4601 1132 Size: 97 815 1012 1545 2229 Fragments arranged by size: 2229 1545 1012 815 97 PasI CC'CwG_GG Cuts at: 1348 2423 1348 Size: 1075 4623 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2015 2047 3767 3799 5280 5312 2015 Size: 32 1720 32 1481 32 2401 Fragments arranged by size: 2401 1720 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2893 1613 Size: 1280 4418 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2306 2306 Size: 5698 PstI C_TGCA'G Cuts at: 830 2352 3050 830 Size: 1522 698 3478 Fragments arranged by size: 3478 1522 698 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2197 2197 Size: 5698 SacI G_AGCT'C Cuts at: 2670 2699 2670 Size: 29 5669 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2348 3046 4181 4862 5053 826 Size: 219 1303 698 1135 681 191 1471 Fragments arranged by size: 1471 1303 1135 698 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2276 2276 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2636 3802 4703 4965 820 Size: 197 926 456 237 1166 901 262 1553 Fragments arranged by size: 1553 1166 926 901 456 262 237 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2280 2491 2898 2982 3137 Size: 552 939 48 228 211 407 84 155 Cuts at: 3137 513 Size: 3074 Fragments arranged by size: 3074 939 552 407 228 211 155 84 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 5658 5690 2684 Size: 32 2942 32 2692 Fragments arranged by size: 2942 2692 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2536 1316 Size: 1220 4478 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 2096 2948 3827 2096 Size: 852 879 3967 Fragments arranged by size: 3967 879 852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI