################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 708 GGTACC G^GTACC AflIII 1971 ACATGT A^C[GA][CT]GT AlwI 760 GGATC GGATC(4/5) AlwNI 1957 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1031 CCTCAGG CC^T[ACGT]AGG AseI 2533 ATTAAT AT^TAAT AsnI 2533 ATTAAT AT^TAAT Asp700I 724 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 708 GGTACC G^GTACC BclI 1562 TGATCA T^GATCA BcnI 2472 CCGGG CC^[GC]GG BseRI 1711 GAGGAG GAGGAG(10/8) BsgI 879 GTGCAG GTGCAG(16/14) BsiEI 2391 CGACCG CG[GA][CT]^CG BsmFI 621 GGGAC GGGAC(10/14) Bsp1407I 1349 TGTACA T^GTACA BspLU11I 1971 ACATGT A^CATGT BspMI 15 ACCTGC ACCTGC(4/8) BsrGI 1349 TGTACA T^GTACA BstXI 2046 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1031 CCTCAGG CC^T[ACGT]AGG DraIII 1678 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1031 CCTCAGG CC^T[ACGT]AGG EcoT14I 1787 CCATGG C^C[AT][AT]GG EcoT22I 947 ATGCAT ATGCA^T FbaI 1562 TGATCA T^GATCA HapII 2472 CCGG C^CGG HincII 2252 GTCAAC GT[CT]^[GA]AC HindII 2252 GTCAAC GT[CT]^[GA]AC HpaII 2472 CCGG C^CGG KpnI 708 GGTACC GGTAC^C MaeII 1076 ACGT A^CGT MspI 2472 CCGG C^CGG NciI 2472 CCGGG CC^[GC]GG NcoI 1787 CCATGG C^CATGG NsiI 947 ATGCAT ATGCA^T NspI 1971 ACATGT [GA]CATG^[CT] PflMI 1677 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2533 ATTAAT AT^TAAT SexAI 2354 ACCTGGT A^CC[AT]GGT SspBI 1349 TGTACA T^GTACA StuI 1383 AGGCCT AGG^CCT StyI 1787 CCATGG C^C[AT][AT]GG Van91I 1677 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 724 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 299 GGTCC G^G[AT]CC 1110 GGACC BanI 516 GGCACC G^G[CT][GA]CC 708 GGTACC BciVI 448 GGATAC GTATCC(6/5) 2285 GTATCC BfaI 217 CTAG C^TAG 1390 CTAG BpmI 231 CTCCAG CTGGAG(16/14) 496 CTCCAG BsmI 142 GAATGC GAATGC(1/-1) 2577 GCATTC BsrDI 33 CATTGC GCAATG(2/0) 1257 CATTGC Cac8I 1603 GCTGGC GC[ACGT]^[ACGT]GC 2363 GCTGGC DsaI 1787 CCATGG C^C[GA][CT]GG 2394 CCGTGG HindIII 329 AAGCTT A^AGCTT 464 AAGCTT MaeI 217 CTAG C^TAG 1390 CTAG NdeI 1127 CATATG CA^TATG 1234 CATATG PstI 17 CTGCAG CTGCA^G 1587 CTGCAG ScaI 831 AGTACT AGT^ACT 2480 AGTACT SfaNI 677 GCATC GCATC(5/9) 1592 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AcsI AfaI AluI ApoI BbsI BbvI BpuAI BsaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I