(Circular) (Six-base) MAPSORT of: pFN21AB8621.seq Check: 8014 from: 1 to: 7315 pFN21AB8621 7315 bp With 182 enzymes: SgfI * March 28, 2012 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5125 278 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4658 1937 Size: 2721 4594 Acc65I G'GTAC_C Cuts at: 2713 4636 2713 Size: 1923 5392 AclI AA'CG_TT Cuts at: 1563 5442 5815 1563 Size: 3879 373 3063 Fragments arranged by size: 3879 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2837 5378 6762 7030 1632 Size: 1205 2541 1384 268 1917 Fragments arranged by size: 2541 1917 1384 1205 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7118 AflIII A'CryG_T Cuts at: 1203 3976 6214 7191 1203 Size: 2773 2238 977 1327 Fragments arranged by size: 2773 2238 1327 977 AgeI A'CCGG_T Cuts at: 6146 6146 Size: 7315 AhdI GACnn_n'nnGTC Cuts at: 6044 6044 Size: 7315 AleI CACnn'nnGTG Cuts at: 1166 2795 2881 3059 1166 Size: 1629 86 178 5422 Fragments arranged by size: 5422 1629 178 86 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7315 AlwNI CAG_nnn'CTG Cuts at: 3967 5058 6630 3967 Size: 1091 1572 4652 Fragments arranged by size: 4652 1572 1091 ApaLI G'TGCA_C Cuts at: 5372 6528 5372 Size: 1156 6159 ApoI r'AATT_y Cuts at: 1493 2008 2227 2731 2752 3301 3319 3333 3438 Size: 515 219 504 21 549 18 14 105 Cuts at: 3438 3474 4184 4472 4624 4850 5002 7111 1493 Size: 36 710 288 152 226 152 2109 1697 Fragments arranged by size: 2109 1697 710 549 515 504 288 226 219 152 152 105 36 21 18 14 AseI AT'TA_AT Cuts at: 160 1032 4539 5869 160 Size: 872 3507 1330 1606 Fragments arranged by size: 3507 1606 1330 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 AvaI C'yCGr_G Cuts at: 1870 1943 4640 1870 Size: 73 2697 4545 Fragments arranged by size: 4545 2697 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4273 4306 4273 Size: 33 7282 BamHI G'GATC_C Cuts at: 4645 5012 4645 Size: 367 6948 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2521 2713 4636 6091 618 Size: 325 217 627 734 192 1923 1455 1842 Fragments arranged by size: 1923 1842 1455 734 627 325 217 192 BanII G_rGCy'C Cuts at: 1391 4634 1391 Size: 3243 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 4069 4183 928 Size: 971 766 1404 114 4060 Fragments arranged by size: 4060 1404 971 766 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5506 5540 1903 Size: 34 3569 34 3678 Fragments arranged by size: 3678 3569 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2447 4301 5207 6417 2447 Size: 1854 906 1210 3345 Fragments arranged by size: 3345 1854 1210 906 BclI T'GATC_A Cuts at: 1548 3567 1548 Size: 2019 5296 BfrBI ATG'CAT Cuts at: 2954 2954 Size: 7315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5926 136 Size: 107 122 71 631 605 4254 1525 Fragments arranged by size: 4254 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7310 7310 Size: 7315 BlpI GC'TnA_GC Cuts at: 4732 4732 Size: 7315 Bme1580I G_kGCm'C Cuts at: 1790 5376 6532 1790 Size: 3586 1156 2573 Fragments arranged by size: 3586 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5046 6004 442 Size: 416 53 83 399 24 3629 958 1753 Fragments arranged by size: 3629 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2486 5975 1239 Size: 982 265 3489 2579 Fragments arranged by size: 3489 2579 982 265 Bpu10I CC'TnA_GC Cuts at: 3120 3120 Size: 7315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5440 6305 6603 5440 Size: 865 298 6152 Fragments arranged by size: 6152 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2792 3198 3951 5978 882 Size: 617 1293 406 753 2027 2219 Fragments arranged by size: 2219 2027 1293 753 617 406 BsaAI yAC'GTr Cuts at: 493 1204 5106 493 Size: 711 3902 2702 Fragments arranged by size: 3902 2702 711 BsaBI GATnn'nnATC Cuts at: 7309 7309 Size: 7315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5122 5504 6139 275 Size: 53 83 186 936 3589 382 635 1451 Fragments arranged by size: 3589 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5748 6146 6420 6567 6978 5748 Size: 398 274 147 411 6085 Fragments arranged by size: 6085 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 1178 Size: 30 165 30 1216 30 5844 Fragments arranged by size: 5844 1216 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3731 1186 Size: 2545 4770 BseYI C'CCAG_C Cuts at: 3468 6518 7014 3468 Size: 3050 496 3769 Fragments arranged by size: 3769 3050 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2905 1163 Size: 573 1169 5573 Fragments arranged by size: 5573 1169 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 4765 5526 6554 1540 Size: 275 186 2398 366 761 1028 2301 Fragments arranged by size: 2398 2301 1028 761 366 275 186 BsiHKAI G_wGCw'C Cuts at: 5376 5461 6532 5376 Size: 85 1071 6159 Fragments arranged by size: 6159 1071 85 BsmI GAATG_Cn' Cuts at: 2153 4582 4826 4919 2153 Size: 2429 244 93 4549 Fragments arranged by size: 4549 2429 244 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4634 5376 5461 6532 1391 Size: 399 2844 742 85 1071 2174 Fragments arranged by size: 2844 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6978 6978 Size: 7315 BspHI T'CATG_A Cuts at: 5204 6934 5204 Size: 1730 5585 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 4670 844 Size: 1185 2641 3489 Fragments arranged by size: 3489 2641 1185 BsrBI CCG'CTC Cuts at: 1394 1823 5202 1394 Size: 429 3379 3507 Fragments arranged by size: 3507 3379 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 3261 5810 5984 65 Size: 1183 789 1224 2549 174 1396 Fragments arranged by size: 2549 1396 1224 1183 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5959 6146 1700 Size: 110 4149 187 2869 Fragments arranged by size: 4149 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3354 96 Size: 3258 4057 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7315 BssSI C'ACGA_G Cuts at: 5375 6387 5375 Size: 1012 6303 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7315 BstBI TT'CG_AA Cuts at: 6119 6119 Size: 7315 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7315 BstXI CCAn_nnnn'nTGG Cuts at: 4058 4058 Size: 7315 BstYI r'GATC_y Cuts at: 1972 4645 5012 5397 5414 6866 6952 6964 7310 Size: 2673 367 385 17 1452 86 12 346 Cuts at: 7310 1972 Size: 1977 Fragments arranged by size: 2673 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3037 3037 Size: 7315 BtgI C'CryG_G Cuts at: 513 1065 3792 4399 513 Size: 552 2727 607 3429 Fragments arranged by size: 3429 2727 607 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2668 487 Size: 683 56 1442 5134 Fragments arranged by size: 5134 1442 683 56 BtsI GCAGTG_nn' Cuts at: 3811 4831 5625 5645 3811 Size: 1020 794 20 5481 Fragments arranged by size: 5481 1020 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7280 DraI TTT'AAA Cuts at: 4619 4619 Size: 7315 DraIII CAC_nnn'GTG Cuts at: 3688 3688 Size: 7315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6322 809 Size: 507 5006 1802 Fragments arranged by size: 5006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4762 5651 7280 Size: 54 1425 50 275 18 2932 889 1629 Cuts at: 7280 8 Size: 43 Fragments arranged by size: 2932 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4762 1537 Size: 275 2950 4090 Fragments arranged by size: 4090 2950 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5245 1353 Size: 3892 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5902 6276 6422 387 Size: 1064 138 4313 374 146 1280 Fragments arranged by size: 4313 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2486 2837 5378 5975 6762 7030 Size: 393 589 265 351 2541 597 787 268 Cuts at: 7030 1239 Size: 1524 Fragments arranged by size: 2541 1524 787 597 589 393 351 268 265 EcoRI G'AATT_C Cuts at: 2008 3474 4184 4472 4624 5002 2008 Size: 1466 710 288 152 378 4321 Fragments arranged by size: 4321 1466 710 378 288 152 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4403 4403 Size: 7315 FspI TGC'GCA Cuts at: 5821 5821 Size: 7315 HaeII r_GCGC'y Cuts at: 1678 6462 1678 Size: 4784 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2619 2651 2979 3011 3178 Size: 32 135 32 1047 32 328 32 167 Cuts at: 3178 3210 4571 4603 5956 5988 6030 6062 1373 Size: 32 1361 32 1353 32 42 32 2626 Fragments arranged by size: 2626 1361 1353 1047 328 167 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4259 4659 4905 669 Size: 988 281 2321 400 246 3079 Fragments arranged by size: 3079 2321 988 400 281 246 HindIII A'AGCT_T Cuts at: 748 2334 2469 748 Size: 1586 135 5594 Fragments arranged by size: 5594 1586 135 HpaI GTT'AAC Cuts at: 4905 4905 Size: 7315 KpnI G_GTAC'C Cuts at: 2717 4640 2717 Size: 1923 5392 MfeI C'AATT_G Cuts at: 4914 4914 Size: 7315 MluI A'CGCG_T Cuts at: 7191 7191 Size: 7315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3307 3427 4419 4474 4579 6429 6613 1305 Size: 2002 120 992 55 105 1850 184 2007 Fragments arranged by size: 2007 2002 1850 992 184 120 105 55 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5493 Fragments arranged by size: 5493 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2795 2881 3059 3626 5273 5632 5791 Size: 648 1629 86 178 567 1647 359 159 Cuts at: 5791 518 Size: 2042 Fragments arranged by size: 2042 1647 1629 648 567 359 178 159 86 MspA1I CmG'CkG Cuts at: 1606 4731 5408 6556 6801 1606 Size: 3125 677 1148 245 2120 Fragments arranged by size: 3125 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3792 513 Size: 552 2727 4036 Fragments arranged by size: 4036 2727 552 NdeI CA'TA_TG Cuts at: 387 3133 3240 387 Size: 2746 107 4462 Fragments arranged by size: 4462 2746 107 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7315 NotI GC'GGCC_GC Cuts at: 4762 4762 Size: 7315 NsiI A_TGCA'T Cuts at: 2956 2956 Size: 7315 NspI r_CATG'y Cuts at: 1132 1229 3980 4673 6218 1132 Size: 97 2751 693 1545 2229 Fragments arranged by size: 2751 2229 1545 693 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7315 PciI A'CATG_T Cuts at: 3976 6214 3976 Size: 2238 5077 PflMI CCAn_nnn'nTGG Cuts at: 1108 3688 1108 Size: 2580 4735 PfoI T'CCnGG_A Cuts at: 4476 4476 Size: 7315 PmeI GTTT'AAAC Cuts at: 4619 4619 Size: 7315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5384 5416 6897 6929 5384 Size: 32 1481 32 5770 Fragments arranged by size: 5770 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7315 PsiI TTA'TAA Cuts at: 3032 4885 3032 Size: 1853 5462 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3930 3962 3930 Size: 32 7283 PstI C_TGCA'G Cuts at: 830 2026 3596 4667 830 Size: 1196 1570 1071 3478 Fragments arranged by size: 3478 1570 1196 1071 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7315 SalI G'TCGA_C Cuts at: 1936 4657 1936 Size: 2721 4594 SbfI CC_TGCA'GG Cuts at: 4667 4667 Size: 7315 ScaI AGT'ACT Cuts at: 2838 4487 5563 7275 2838 Size: 1649 1076 1712 2878 Fragments arranged by size: 2878 1712 1649 1076 SexAI A'CCwGG_T Cuts at: 4359 4359 Size: 7315 SfcI C'TryA_G Cuts at: 826 1045 2022 2067 2460 2550 2976 3367 3592 Size: 219 977 45 393 90 426 391 225 Cuts at: 3592 3947 4663 5798 6479 6670 826 Size: 355 716 1135 681 191 1471 Fragments arranged by size: 1471 1135 977 716 681 426 393 391 355 225 219 191 90 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7315 SmaI CCC'GGG Cuts at: 1872 4642 1872 Size: 2770 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5419 6320 6582 820 Size: 197 926 3476 901 262 1553 Fragments arranged by size: 3476 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5106 493 Size: 4613 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7315 SphI G_CATG'C Cuts at: 1132 4673 1132 Size: 3541 3774 SspI AAT'ATT Cuts at: 5 52 5239 5 Size: 47 5187 2081 Fragments arranged by size: 5187 2081 47 StuI AGG'CCT Cuts at: 1517 3390 1517 Size: 1873 5442 StyI C'CwwG_G Cuts at: 513 1065 3792 4754 513 Size: 552 2727 962 3074 Fragments arranged by size: 3074 2727 962 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5342 5359 5512 5697 6142 325 Size: 1255 3762 17 153 185 445 1498 Fragments arranged by size: 3762 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 2505 2836 Size: 274 80 33 51 1443 301 227 331 Cuts at: 2836 3354 4485 5561 7273 96 Size: 518 1131 1076 1712 138 Fragments arranged by size: 1712 1443 1131 1076 518 331 301 274 227 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7275 7307 7275 Size: 32 7283 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7315 XbaI T'CTAG_A Cuts at: 4651 4651 Size: 7315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7315 XmaI C'CCGG_G Cuts at: 1870 4640 1870 Size: 2770 4545 XmnI GAAnn'nnTTC Cuts at: 2733 5444 2733 Size: 2711 4604 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5123 276 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI