(Linear) (Six-base) MAPSORT of: KIBB8621.seq Check: 7415 from: 1 to: 2610 KIBB8621.orf With 182 enzymes: SgfI * March 22, 2012 15:43 .. Acc65I G'GTAC_C Cuts at: 0 708 2610 Size: 708 1902 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 832 2610 Size: 832 1778 AflIII A'CryG_T Cuts at: 0 1971 2610 Size: 1971 639 AleI CACnn'nnGTG Cuts at: 0 790 876 1054 2610 Size: 790 86 178 1556 Fragments arranged by size: 1556 790 178 86 AlwNI CAG_nnn'CTG Cuts at: 0 1962 2610 Size: 1962 648 ApoI r'AATT_y Cuts at: 0 3 222 726 747 1296 1314 1328 1433 Size: 3 219 504 21 549 18 14 105 Cuts at: 1433 1469 2179 2467 2610 Size: 36 710 288 143 Fragments arranged by size: 710 549 504 288 219 143 105 36 21 18 14 3 AseI AT'TA_AT Cuts at: 0 2534 2610 Size: 2534 76 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2268 2301 2610 Size: 2268 33 309 Fragments arranged by size: 2268 309 33 BanI G'GyrC_C Cuts at: 0 516 708 2610 Size: 516 192 1902 Fragments arranged by size: 1902 516 192 BbsI GAAGACnn'nnnn_ Cuts at: 0 660 2064 2178 2610 Size: 660 1404 114 432 Fragments arranged by size: 1404 660 432 114 BciVI GTATCCnnnnn_n' Cuts at: 0 442 2296 2610 Size: 442 1854 314 Fragments arranged by size: 1854 442 314 BclI T'GATC_A Cuts at: 0 1562 2610 Size: 1562 1048 BfrBI ATG'CAT Cuts at: 0 949 2610 Size: 949 1661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 481 2610 Size: 216 265 2129 Fragments arranged by size: 2129 265 216 Bpu10I CC'TnA_GC Cuts at: 0 1115 2610 Size: 1115 1495 BsaI GGTCTCn'nnnn_ Cuts at: 0 787 1193 1946 2610 Size: 787 406 753 664 Fragments arranged by size: 787 753 664 406 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 614 644 2610 Size: 614 30 1966 Fragments arranged by size: 1966 614 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1726 2610 Size: 1726 884 BseYI C'CCAG_C Cuts at: 0 1463 2610 Size: 1463 1147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 900 2610 Size: 900 1710 BsiEI CG_ry'CG Cuts at: 0 2394 2610 Size: 2394 216 BsmI GAATG_Cn' Cuts at: 0 148 2577 2610 Size: 148 2429 33 Fragments arranged by size: 2429 148 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 24 2610 Size: 24 2586 BsrDI GCAATG_nn' Cuts at: 0 32 1256 2610 Size: 32 1224 1354 Fragments arranged by size: 1354 1224 32 BsrGI T'GTAC_A Cuts at: 0 1349 2610 Size: 1349 1261 BstXI CCAn_nnnn'nTGG Cuts at: 0 2053 2610 Size: 2053 557 Bsu36I CC'TnA_GG Cuts at: 0 1032 2610 Size: 1032 1578 BtgI C'CryG_G Cuts at: 0 1787 2394 2610 Size: 1787 607 216 Fragments arranged by size: 1787 607 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 663 2610 Size: 663 1947 BtsI GCAGTG_nn' Cuts at: 0 1806 2610 Size: 1806 804 DraIII CAC_nnn'GTG Cuts at: 0 1683 2610 Size: 1683 927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 481 832 2610 Size: 216 265 351 1778 Fragments arranged by size: 1778 351 265 216 EcoRI G'AATT_C Cuts at: 0 3 1469 2179 2467 2610 Size: 3 1466 710 288 143 Fragments arranged by size: 1466 710 288 143 3 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2398 2610 Size: 2398 212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 614 646 974 1006 1173 1205 2566 2598 Size: 614 32 328 32 167 32 1361 32 Cuts at: 2598 2610 Size: 12 Fragments arranged by size: 1361 614 328 167 32 32 32 32 12 HincII GTy'rAC Cuts at: 0 2254 2610 Size: 2254 356 HindIII A'AGCT_T Cuts at: 0 329 464 2610 Size: 329 135 2146 Fragments arranged by size: 2146 329 135 KpnI G_GTAC'C Cuts at: 0 712 2610 Size: 712 1898 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1302 1422 2414 2469 2574 2610 Size: 1302 120 992 55 105 36 Fragments arranged by size: 1302 992 120 105 55 36 MslI CAynn'nnrTG Cuts at: 0 790 876 1054 1621 2610 Size: 790 86 178 567 989 Fragments arranged by size: 989 790 567 178 86 NcoI C'CATG_G Cuts at: 0 1787 2610 Size: 1787 823 NdeI CA'TA_TG Cuts at: 0 1128 1235 2610 Size: 1128 107 1375 Fragments arranged by size: 1375 1128 107 NsiI A_TGCA'T Cuts at: 0 951 2610 Size: 951 1659 NspI r_CATG'y Cuts at: 0 1975 2610 Size: 1975 635 PciI A'CATG_T Cuts at: 0 1971 2610 Size: 1971 639 PflMI CCAn_nnn'nTGG Cuts at: 0 1683 2610 Size: 1683 927 PfoI T'CCnGG_A Cuts at: 0 2471 2610 Size: 2471 139 PsiI TTA'TAA Cuts at: 0 1027 2610 Size: 1027 1583 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1925 1957 2610 Size: 1925 32 653 Fragments arranged by size: 1925 653 32 PstI C_TGCA'G Cuts at: 0 21 1591 2610 Size: 21 1570 1019 Fragments arranged by size: 1570 1019 21 ScaI AGT'ACT Cuts at: 0 833 2482 2610 Size: 833 1649 128 Fragments arranged by size: 1649 833 128 SexAI A'CCwGG_T Cuts at: 0 2354 2610 Size: 2354 256 SfcI C'TryA_G Cuts at: 0 17 62 455 545 971 1362 1587 1942 Size: 17 45 393 90 426 391 225 355 Cuts at: 1942 2610 Size: 668 Fragments arranged by size: 668 426 393 391 355 225 90 45 17 StuI AGG'CCT Cuts at: 0 1385 2610 Size: 1385 1225 StyI C'CwwG_G Cuts at: 0 1787 2610 Size: 1787 823 TatI w'GTAC_w Cuts at: 0 273 500 831 1349 2480 2610 Size: 273 227 331 518 1131 130 Fragments arranged by size: 1131 518 331 273 227 130 XmnI GAAnn'nnTTC Cuts at: 0 728 2610 Size: 728 1882 Enzymes that do cut: Acc65I AcuI AflIII AleI AlwNI ApoI AseI BaeI BanI BbsI BciVI BclI BfrBI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiEI BsmI BspMI BsrDI BsrGI BstXI Bsu36I BtgI BtgZI BtsI DraIII Eco57MI EcoRI FalI Hin4I HincII HindIII KpnI MmeI MslI NcoI NdeI NsiI NspI PciI PflMI PfoI PsiI PsrI PstI ScaI SexAI SfcI StuI StyI TatI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI